• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.134-142
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• 2008

This work aims to obtain basic information for seed propagation of Hydrangea serrata for. acuminata. The germination percentage of the seeds taken on 15 November, 30 December, and 23 January was $90.0{\pm}4.16%$, $84.4{\pm}5.52%$, and $88.9{\pm}2.40%$, respectively. This suggest that seeds of Hydrangea serrata for. acuminata are non-dormant seeds. The optimum temperature for germination was $25^{\circ}C$ and light was necessary. Most of the growth parameters (shoot and leaf length, stem diameter, root length, no. of roots, T/R ratio, and fresh and dry wts.) were significantly greater at $25/20^{\circ}C$ and $25^{\circ}C$ than at the other temperatures. Low T/R ratio at relatively cool temperatures (15 and $20^{\circ}C$) was caused by suppressed top growth. In light quality treatment, red light (RL) significantly enhanced stem elongation. The greatest photosynthetic pigments (total chl, chl a/b, and carotenoid) were observed in seedlings grown in blue light (BL), followed by seedlings grown in RL+BL. When blue light was added, higher pigment contents were found. Effect of plug cell size (50, 72, 128, 162 and 200 cells) on the growth of seedlings was investigated. The highest top growth was observed in seedlings grown in 50 cell trays, followed by seedlings grown in 72, 128, 162, and 200 cell trays. However, there was no significant differences between 162 and 200 cell trays. Especially, smaller size leaves were observed in seedlings grown in smaller cell trays (lower volume and high plant density).

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.196-196
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• 2017

Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.9
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• pp.1233-1242
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• 2011

The in vivo digestibility of mulberry leaves (Morus alba) and the effects of the partial replacement of cotton seed with fresh mulberry leaf in rations on the performance of growing Vietnamese cattle was investigated. For the in vivo digestibility trial, twenty castrated rams of Phanrang breed (a local prolific breed) with an initial weight of 23-25 kg, were first assigned to four groups according to weight and then randomly assigned to one of four dietary treatments to determine digestibility of nutrients in mulberry leaves (M. alba), natural Bermuda grass (Cynodon dactylon), elephant grass (Pennisetum purpureum) and buffalo grass (Panicum maximum cv. TD 58). All forages were cut and chopped daily before being offered (at 120% maintenance) to the sheep. In the feeding trial, 20 Laisind (Vietnam yellow cows${\times}$Red Sindhy bulls) crossbred bulls averaged 18 month old and 184 kg were used to investigate the effect of partial replacement of cottonseed in the diet by mulberry leaves on live weight gain and feed conversion rate. The experiment was a randomized complete block design with four levels of fresh mulberry leaves which varied from 0 to 15% of total dietary dry mater and five animals per treatment over an 84 day period. The in vivo digestion trial showed the superior quality of mulberry leaves compared with the grasses. Chemical analysis indicated that mulberry leaves had the highest CP and the lowest NDF contents (22.3 and 31.1% DM, respectively) among the four forages tested. Digestibility of DM and OM of the mulberry leaf (66.4 and 71.8%, respectively) was also the highest but that of CP (58.2%) and NDF (58.4%) was the lowest of the four forages evaluated (p<0.05). Consequently, the ME value and therefore net energy (NE) and unit feed for lactation (UFL) values of the mulberry leaves, which was estimated from chemical composition and digestibility values, were the highest among the forages investigated in the present study. Results of the feeding trial showed no treatment effect on average daily gain (ADG) of the cattle. The values were 554, 583, 565 and 568 g/d for animals in the diets of 0, 5, 10, and 15% mulberry leaves inclusion, respectively. Total DM intake of the animal was not affected by the treatment when expressed as kg/animal/d. However, when adjusted for metabolic weight of the animal the DM intake was reduced (p<0.05) as whole cottonseed was replaced by mulberry leaves in the ration. When the level of mulberry leaves in the ration increased from 5 to 15% of dietary DM at the expense of whole cottonseed, CP and ME intakes of the cattle were significantly decreased (p<0.05) and the feed to gain ratio reduced by 8 to 14% as compared with the control diet (p<0.05). Mulberry leaf is a good feed ingredient for ruminants because of its high level of crude protein and high digestibility of nutrients and energy. Mulberry leaves can be efficiently used as a source of protein supplement to replace cottonseed, a more expensive animal feeds ingredient, in the diet for Vietnamese cattle.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.100-106
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• 2014

In order to develop an efficient in vitro micropropagation technique for a rare plant species, Astragalus membranaceus Bunge var. alpinus N., shoot proliferation and in vitro or in vivo rootings were conducted and hyperhydrated leaf generated from cultures was histologically observed. During shoot induction, no distinct effect on multiple shoot induction was found between BA and kinetin treatment. BA enhanced the number of internodes, whereas kinetin stimulated shoot elongation. Hyperhydrated leaf composed of bigger cells and retarded palisade parenchyma and showed irregular cell arrangement compared to normal leaf. Especially starch content in hyperhydrated leaf was significantly reduced. The best rooting rate was achieved by B5 medium among three different medium (B5, MS and WPM) and 0.1mg/L IBA treatment induced the highest rooting ratio (80%). No statistical difference was induced by explant types (apical bud or axillary bud) in terms of rooting ratio. In vivo cutting induced rooting rate up to 65% by 0.5% IBA/Talc powder treatment. Although in vivo rooting rate was less efficient compared to in vitro rooting, better survival rate was observed after soil acclimatization. Present study suggested that above micropropagation techniques can be used for rapid multiplication as well as in vitro or in vivo conservation of the species.

• Korean Journal of Environmental Agriculture
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• v.15 no.1
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• pp.11-18
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• 1996

A greenhouse experiment on the Sr-90 uptake by peanut and sesame was conducted through pot cultures on a sandy loam soil of pH 6.35 treated with Sr-90 in 5.2 and 31.2Bq per gram. The rate of Sr-90 transfer from soil to each plant part, the ratio of Sr-90 concentration in the part to the concentration in soil, and the patterns of their temporal changes were not, on the whole, significantly different between the two treatments. About 0.7 and 0.5 % of Sr-90 in soil transferred to all the mature plants of peanut and sesame, respectively, with the radioactivities in their roots not counted. Only 4% and less than 15% of Sr-90 absorbed by peanut and sesame, respectively, translocated to their seeds. Both crops showed the highest Sr-90 concentration in the leaf and the lowest in the seed. At maturities, the concentration ratio in dry seed was 0.4 in peanut and 3.3 in sesame and that in dry leaf was 12.5 and 10.7, respectively. Sr-90 concentrations in the top 15 cm soil after harvests averaged about 80 % of the concentrations at starting. Sr-90 uptake resulted in neither growth inhibition nor yield decrease.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.267-271
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• 2010

A new black seed coat soybean cultivar, 'Daeheug', was developed at the National Institute of Crop Science (NICS) in 2007. The breeding goal for black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Daeheug' was selected from the cross between 'Daehwang' which had yellow seed coat, large seed size, and late maturity, and 'Milyang 79' which was lodging tolerant, and SMV resistant with medium seed size, black seed coat, and yellow cotyledon. The preliminary, advanced and regional yield trials for evaluation and selection of this cultivar were carried out from 2003 to 2007. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, spherical flattened seed shape, oval leaf shape and large seed size (34.3 g/100 seeds). In maturity, 'Daeheug' was 2 days later than the check cultivar 'Ilpumgeomjeongkong'. In the seed quality such as isoflavone contents ($635{\mu}g/g$), and anthocyanin contents ($11.2m{\ell}/g$ of seed coat), 'Daeheug' was better than the check cultivar. It has good adaptability for cooking with rice in ratio of water absorption and seed hardness. Specially, it has good processability for soybean tea with 2.13 absorbance at 530 nm, scavenge activity of DPPH radical was high, and contents of total phenolics was $1,263{\mu}g/m{\ell}$. The average yield of 'Daeheug' was 2.26 ton per hectare in the regional yield trials (RYT) carried out in six locations of Korea from 2005 to 2007.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.2
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• pp.94-99
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• 2013

Yanganok, a new single cross variety, was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2012. This hybrid, which has a high yield of grain was produced by crossing two inbred lines, KS164 and KS163. KS164 is the seed parent and KS163 is the pollen parent of Yanganok. It is a yellow-orange intermediate maize hybrid (Zea mays L.). After a preliminary yield trial and advanced yield trial of Yanganok (Suwon180) in Suwon for 2 years, a regional yield trial was carried out for its growth characteristics, and yields were determined at 4 different locations from 2009 to 2012, with the exception of 2010. It was named 'Yanganok'. The silking date of Yanganok is 2 days earlier than that of the check hybrid, Kwangpyeongok. Plant height of Yanganok is similar to Kwangpyeongok, but its ear height ratio is higher than that of Kwangpyeongok. It has resistance to lodging. Stay-green features of Yanganok are similar to those of Kwangpyeongok. The ear ratio of Yanganok is similar to Kwangpyeongok. It has moderate resistance to southern leaf blight (Bipolaris maydis) and strong resistance to northern leaf blight (Exserohilum turcicum). It has moderate resistance to black streaked dwarf virus (BSDV), ear lot and corn borer. The dry matter yield of Yanganok, 17.45 ton/ha, was similar to that of Kwangpyeongok. The total digestible nutrients (TDN) yield of Yanganok, 11.96 ton/ha, was similar to that of Kwangpyeongok. The grain yield of Yanganok, 8.32 ton/ha was similar to that of Jangdaok. Seed production of Yanganok went well due to a good match during crossing between the seed parent, KS164, and the pollen parent, KS163, in Yeongwol, and F1 seed yield was 1.79 ton/ha. Yanganok would be a suitable cultivar to all plain areas in the Korean peninsula.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.668-673
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• 2010

A new black seed coat soybean cultivar, 'Heugseong' was developed at the National Institute of Crop Science (NICS) in 2008. The goal to breed the black seed coat soybean is to develop the cultivar with large seed size, high yield, lodging tolerance, and resistance to disease such as soybean mosaic virus (SMV), and bacterial pustule. 'Heugseong' was selected from the cross between 'Gnome85', showing yellow seed coat, lodging tolerance, and high yield, and 'Cheongja', showing green cotyledon, and black seed coat. The preliminary, advanced and regional yield trials for this cultivar were carried out from 2004 to 2008. It has determinate growth habit, purple flower, brown pubescence, brown pod color, black seed coat, yellow cotyledon, elongated seed shape, oval leaf shape and large seed size (29.2 grams per 100 seeds). It was 4 days later in maturity than the check cultivar 'Ilpumgeomjeongkong'. 'Heugseong' was better than the check cultivar in the seed quality of isoflavone contents(1,913 ${\mu}g/g$). It has good adaptability for cooking with rice in ratio of water absorption and seed hardness, for physics of black tofu, and for fermented black soybean paste. The average yield of 'Heugseong' was 2.37 ton per hectare in the regional yield trials carried out in six locations of Korea from 2006 to 2008.

• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.990-998
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• 2012

This study investigated the effects of the diet with Cucurbita moschata (CM), adlay seed (AS), and Cudrania tricuspidata leaf (CTL) mixed-powder on the visceral fat, fecal amount, and serum lipid profiles in rats fed a high-fat diet (HFD). The mixed ratio of CM, AS, and CTL as a functional food ingredient was 1:1:1 on a weight basis. Male Sprague-Dawley rats were fed a high-fat diet (D12492; 45 kcal fat) for 5 weeks during the first phase. In the second phase, which lasted 5 weeks, the rats were divided into six experimental groups. The groups were: a high-fat diet group as a control during the 10 weeks of experimental period; and a high-fat diet with 5, 10, 15, 20, and 30% mixed-powder supplement group. The adipose tissue (RFP, EFP) weights were significantly decreased in the 20% and 30% mixed-powder supplement groups than in the HFD group (p<0.05). The fecal weight produced by the 15%, 20%, and 30% mixed-powder supplement groups was significantly increased during the second phase compared to the first phase (p<0.05). In terms of serum lipids, TG (triglyceride) content was decreased in the 10% and 15% mixed-powder supplement groups. The blood glucose levels were significantly decreased in all experimental diet supplement groups than in the HFD group (p<0.05). The findings show that the high supplement groups could improve the visceral fat accumulation, fecal amount, and blood glucose content compared to the HFD group.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.86-92
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• 1992

Green buckwheat seedlings have been used as a pollution free vegetable and medicinal crop for a long time. Some of the reasons are the rapid growth rate, the high protein and rutincontent, and a more favorable ratio of leaf to stem than mature plants. Off-crop season cultivation techniques were developed for growing the young and green buckwheat vegetable of higher quality and yield, and for its increased value-added income. The effects of planting season, seeding rate, seed bed soil fertility and type, and seedling growth period on yield and rutin content were determined for vegetable and medicinal use. The young vegetable yields. in the off season culture ranged from 2.62t/ha to 22.7t /ha. The highest vegetable yield was 22.7t /ha for 25 days old seedlings grown in the polyethylene film tunnel from March 30 to April 25, 1991 where seedling rate was 360kg /ha. Buckwheat vegetable quality and income were dependent upon planting season, seeding rate, growing duration and temperature, and facilities of raising seedlings. Protein content of buck-wheat seedlings was from 21.5% to 17.2%. Rutin content of the vegetable was 53.9～31.7mg /100g for the whole plant in average. The protein and rutin content was significantly varied due to the different environmental conditions including fertility and type of seed bed soil, growing duration, temperature of the green house, and polyethylene film house and tunnel.