• 한국연초학회지
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• 제13권1호
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• pp.27-35
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• 1991

These studies were conducted to investigate the changes of starch, sugar and nicotine content in relation to maturity and cultural conditions and correlation between growth characteristics and chemical components in order to improve the leaf quality. The results were summarized as follows; 1. Nicotine content, total nitrogen content and the ratio of total nitrogen to nicotine of the green leaf were increased with heavier fertilizer, wider planting space and lower topping, whereas starch, total sugar and the ratio of sugar to nicotine were decreased. 2. Starch content, sugar content and the ratio of total sugar to nicotine(T-S/Nic) in the cured leaf were decreased with milder planting space, less application of MH and delaying harvesting date. 3. It was estimated that 58.1% of starch of the harvested leaf was converted into sugar of the cured leaf, and also they were significantly correlated each other.

• 한국연초학회지
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• 제8권2호
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• pp.3-8
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• 1986

This study was carried out to investigate the effect of different soil moisture contents on the growth and chemical constituents of burley tobacco and on the protein pattern in tobacco leaf. Height, stem diameter, and largest leaf length of tobacco droughted from 45 to 60 days after transplanting was not recovered by rewatered amount of water supply from 60 to 75 days after transplanting, but leaf width enlarged. Dry weight per unit leaf area and total nitrogen content showed high values in low soil moisture, but total alkaloid contents were not different according to soil moisture contents. Soil moisture content didn't effect on the protein pattern of middle and upper leaves, but lower leaves showed the mild color and fewer numbers of the protein bands than those of midd1e and upper leaves.

• BMB Reports
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• 제32권2호
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• pp.203-209
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• 1999

Three-week grown Arabidopsis thaliana leaves were wounded by cutting whole leaves with a razor blade into pieces (about$3\;mm\;{\times}\;3\;mm$) submerged in various solutions, and incubated in a growth chamber for 24 h. We measured and compared activities of several enzymes such as phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), thioredoxin, thioredoxin reductase, thioltransferase, glutathione reductase, and $NADP^+$ -malate dehydrogenase. PAL activity was decreased in $HgCl_2$-, $CdCl_2$-, and glyphosate-treated leaf slices, and could not be detected after treatment with $CdCl_2$. TAL activity was found to be maximal in the $CdCl_2$-treated leaf slices. Activity of thioredoxin, a small protein known as a cofactor of ribonucleotide reductase and a regulator of photosynthesis, was significantly increased in the $CdCl_2$-treated leaf slices, while thioredoxin reductase activity was maximal in the $HgCl_2$-treated leaf slices. Thioltransferase and glutathione reductase activities were significantly decreased in the $HgCl_2$-treated leaf slices. $NADP^+$ -malate dehydrogenase activity remained relatively constant after the chemical treatments. Our results strongly indicate that sulfhydryl-related and phenylpropanoid-synthesizing enzyme activities are affected by chemical treatments such as hydrogen peroxide, heavy metals, and glyphosate.

• 한국식품영양학회지
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• 제13권1호
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• pp.6-12
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• 2000

This study was performed to investigate the changes in chemical composition of Panax ginseng leaf by harvesting at July, August and September. The levels of crude protein of dried ginseng leaf were decreased from 17.12mg% to 14.26% by harvesting month, however, crude fat contents of dried ginseng leaf were increased slightly from 1.90% to 2.49%. Three kinds of free sugar, i.e. glucose, fructose and sucrose were found in dried ginseng leaf and maltose was not found. Free sugar contents were increased by delaying harvest, but free amino acid were decreased. Total free amino acid was decreased in delayed harvesting month, serine was revealed superior in free amino acid composition, and valine was revealed next order. In minerals, contents of Ca were from 1,306.1mg% to 1,923mg%, that of K were higher than others patricualy as 1,266.9∼1,216.0mg%. The contents of minerals were existence in order of Mg, P, Na, Fe, Mn, Zn and Cu, abundantly. Total vitamin C were present of 391.0∼336.1mg%, and the contents were decreased as delayed as harvesting period despite of the plentiful content. In fatty acid composition of ginseng leaf, the palmitic acid content was as 40% higher than other fatty acids, remarkably.

• 한국연초학회지
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• 제24권2호
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• pp.88-93
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• 2002

This study was carried out to investigate the changes of chromatic, chemical and organoleptic characteristics of green leaf tobaccos during storage in warehouse. Eight grades(flue-cured ; A2O, B2O, C2L and D2L, burley ; A2T, B2T, C2W and D2W) of green loaves produced in 2001 were stored during 8 months(Oct. 30, 2001 to Jun. 30, 2002) in warehouse of Kimcheon(flue-cured) and Namwon(burley) Leaf Tobacco Processing factory, respectively. Moisture contents of D2L in flue-cured and four grades in burley were significantly decreased during storage in warehouse. Redness(a) values of six grades except for D2L and D2W were significantly increased during storage. Lightness(L), yellow(b) and pH values, and the contents of nicotine, total nitrogen and total sugar were not changed during storage. Irritations of flue-cured tobacco were increased, whereas tastes were decreased during storage. Irritations and tastes of burley tobacco were little changed during storage. The result suggests that the flue-cured green leaf being processed as early as possible for minimizing the deterioration of smoke taste during storage in warehouse.

• 한국연초학회지
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• 제23권2호
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• pp.149-155
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• 2001

This study was carried out to evaluate the correlations among degree of carbonization, chemical components and chromatic characteristics, and to establish the criterion of carbonizaion in leaf tobacco during aging storage. The flue-cured (B2O) and burley (B2T) leaf tobaccos were redried with conventional practices for each other type, and moisture contents of leaf tobaccos were adjusted to 13, 15, 17 and 19% after redrying. The leaf tobaccos were packed in carton box and stored for 8 months in the controlled-environment rooms($35^{\circ}C$, 65% R.H. and $40^{\circ}C$, 80% R.H.). Degree of carbonization, chemical components, and chromatic characteristics(L ; black-white, a ; red to green, b ; yellow to blue) were invetigated every month. Carbonization Index (C.I.) was established as (equation omitted) in accordance with degree of carbonization in two types. The C.I. value of flue-cured leaf(56.5) for slight carbonization was higher than that of burley leaf(48.0). C.I. and rate of usable leaf, L and b were positively correlated in two types. C.I. was significantly shown positive correlation with pH in two types. The correlations between the predicted and the observed values of slight carbonizating times(month after storage) using C.I. and pH were positive significant in two types. The results suggest that cabonization index may be useful to forecast and prevent the carbonization, and pH may be useful to estimate cabonization indirectly for flue-cured and burley leaves during aging storage.

• The Plant Pathology Journal
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• 제19권4호
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• pp.189-194
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• 2003

The effects of prochloraz on membrane permeability and germination of uredospores of Puccinia recondita were investigated to determine its potential mode of action on wheat leaf rust control activity. Disease control activity of ergosterol biosynthesis inhibitors (EBIs) and their activities on uredospore membrane permeability and germination were examined with wheat leaf rust pathogen, both in vitro and in vivo. While wheat leaf rust was not controlled by prochloraz, electrolytic leakage and spore germination of P. recondita uredospore was the highest with the use of prochloraz among the eight fungicides tested. Prochloraz stimulated uredospore of P. recondita to germinate at a higher ratio. Although certain EBIs, such as hexaconazole, showed excellent control activity, their effects on uredospore membrane permeability and germination was much inferior to prochloraz. Therefore, results of this study suggest that effects of EBIs on membrane permeability and germination of uredospore are not always correlated with their disease control activity.

• Applied Microscopy
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• 제46권2호
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• pp.76-82
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• 2016

Aloe vera has been used in the pharmaceutical, food and cosmetic industry for its therapeutic properties. However, there are not many current studies on the microstructure of A. vera compared to studies on the chemical constituents and health efficacy of A. vera. Therefore, we compared the morphology of an A. vera leaf using an optical microscope, a conventional scanning electron microscope (SEM) and a cryo-SEM. Especially, this study focused on observing the gel in the inner leaf of A. vera, which is challenging using standard imaging techniques. We found that cryo-SEM is most suitable method for the observation of highly hydrated biomaterials such as A. vera without removing moisture in samples. In addition, we found the optimal analytical conditions of cryo-SEM. The sublimation conditions of $-100^{\circ}C$ and 10 minutes possibly enable the surface of the inner leaf of A. vera to be observed in their "near life-like" state with retaining moisture. The experiment was repeated with A. arborescens and A. saponaria to confirm the feasibility of the conditions. The results of this study can be applied towards the basic research of aloe and further extend previous knowledge about the surface structures of the various succulent plants.

• 한국작물학회지
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• 제51권spc1호
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• pp.297-303
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• 2006

Cuticular waxes on tea (Camellia sinensis L.) loaves consisted mainly of alkanes, fatty acids, primary alcohols, triterpenes, and a group of unknown compounds, dominated by primary alcohols and triterpenes. Tea tree accessions used in this study were M-1, M-2, Sakimidori, and Yabukita. For all accessions, the alkane, fatty acid, and primary alcohol constituents consisted of a homologues series, and the major constituents of primary alcohol class were the C28 and C30 homologues. Triterpenes consisted of friedelin, $\beta-amyrin$, and three unidentified ones and friedelin was the most abundant. Leaf area and the total amounts of cuticular waxes per leaf increased with lower leaf position from the apical bud in Yabukita variety. With different leaf position, total wax amount per unit leaf area on the youngest leaves of P1 (the uppermost leaf position) showed the largest amount $(12.80{\mu}g/cm^2)$, and on mature loaves of P2 to P6 ranged from 7.08 to $7.77{\mu}g/cm^2$, and then on the oldest loaves of P7 (the lowest leaf position) remained at an increased level $(17.53{\mu}g/cm^2)$. During leaf development (lower leaf position), the amount of primary alcohols decreased from P1 to P6 and increased at P7, whereas that of triterpenes increased from P1 to P7. The percentage of each wax class in the total wax amount occurred a decrease in primary alcohol and an increase in triterpene, with leaf age.

• 한국의류학회지
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• 제28권3_4호
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• pp.444-451
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• 2004

This study was carried out to investigate the chemical and dyeing properties of Petasites japonicus leaf extract under the various extracting and dyeing conditions such as temperature, time, the ratio of water and pH, repetition of dyeing in silk and cotton fabrics with Petasites japonicus leaf. The results were as follows: 1. It was found that λ$_{\max}$ of color solution extracted by Petasites japonicus leaf has two peaks at 290nm and 323nm. 2. The optimum extracting conditions were studied at 10$0^{\circ}C$, 40min., pH 7 and 1 : 20(the ratio of water and Petasites japonicus leaf), the optimum dyeing temperature, dyeing time, dyeing pH and repetition of dyeing were 10$0^{\circ}C$, 60min., pH 7, repetitions of three times, respectively. 3. Silk and cotton fabrics dyed with Petasites japonicus leaf extract were colored yellowish orange. The colory Petasites japonicus leaf extract in silk and cotton fabrics were deeped by same-mordanting with aluminum potassium sufate and cupric sulfate. 4. Washing fastness of silk fabrics was 4∼5 grade, but cotton fabrics was 3∼4 grade, so washing fastness of silk fabrics washed with neutral detergent was excellent.