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Single-minded 1 Gene Mapping and Its Variants Association with Growth, Carcass Composition and Meat Quality Traits in the Pig

  • Zhao, X.F.;Xu, N.Y.;Chen, Z.;Wang, Q.;Guo, X.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.7
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    • pp.941-946
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    • 2008
  • Single-minded 1 gene (SIM1) is a homolog of Drosophila SIM1 gene which plays a key role in the midline cell lineage of the central nervous system and is implicated in the regulation of feeding behavior and obesity in the human and mouse. In this study, porcine SIM1 gene was firstly mapped to chromosome 1p13 using radiation hybrid (RH) mapping and two polymorphisms were detected at position 607 (A/G) in SIM1 intron7 and position 780 (C/T) in SIM1 exon8. The last substitution was genotyped in a 364 F2 animal-population and an association analysis of these genotypes was performed with growth, carcass and meat quality traits by the statistical animal model. The results showed the significant influence of the SIM1 genotype on growth (p<0.05): live weight at birth, later period of growth and average daily gain; and effects on carcass composition (p<0.05): weight of head and buck kneed foreleg, backfat depth, loin eye area, carcass leaf fat and ham fat weights; and traits related to intramuscular fat content (p<0.05).

Bioinspired superhydrophobic steel surfaces

  • Heo, Eun-Gyu;O, Gyu-Hwan;Lee, Gwang-Ryeol;Mun, Myeong-Un
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.509-509
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    • 2011
  • Superhydrophobic surfaces on alloyed steels were fabricated with a non-conventional method of plasma etching and subsequent water immersion procedure. High aspect ratio nanopatterns of nanoflake or nano-needle were created on the steels with various Cr content in its composition. With CF4 plasma treatment in radio-frequence chemical vapor deposition (r.-f. CVD) method, steel surfaces were etched and fluorinated by CF4 plasma, which induced the nanopattern evolution through the water immersion process. It was found that fluorine ion played a role as a catalyst to form nanopatterns in water elucidated with XPS and TEM analysis. The hierarchical patterns in micro- and nano scale leads to superhydrophobic properties on the surfaces by deposition of a hydrophobic coating with a-C:H:Si:O film deposited with a gas precursor of hexamethlydisiloxane (HMDSO) with its lower surface energy of 24.2 mN/m, similar to that of curticular wax covering lotus surfaces. Since this method is based on plasma dry etching & coating, precise patterning of surface texturing would be potential on steel or metal surfaces. Patterned hydrophobic steel surfaces were demonstrated by mimicking the Robinia pseudoacacia or acacia leaf, on which water was collected from the humid air using a patterned hydrophobicity on the steels. It is expected that this facile, non-toxic and fast technique would accelerate the large-scale production of superhydrophobic engineering materials with industrial applications.

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Coating rice with mulberry leaves rich in deoxynojirimycin ameliorates hyperglycemia and dyslipidemia in C57BL/KsJ db/db mice

  • Lee, Joomin;Lee, Hyun-Joo;Lee, Jae-Joon
    • Nutrition Research and Practice
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    • v.12 no.6
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    • pp.469-478
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    • 2018
  • BACKGROUND/OBJECTIVES: Mulberry leaf (ML) has been shown to have an inhibitory effect on ${\alpha}$-glucosidase, and suppresses postprandial hyperglycemia, which may be related to its deoxynojirimycin (DNJ) content. This study was conducted to investigate the hypoglycemic and dyslipidemic effects of rice coated with ML rich in DNJ in a type 2 diabetes mouse model. MATERIALS/METHODS: The mice were divided into four groups (n = 8 each): non-diabetic normal control (NC); diabetic control (DM-C), fed with 10% polished rice powder (DM-R); and fed with 10% polished rice powder coated with DNJ-rich ML (DM-DNJR). RESULTS: Supplementation with DNJR for six weeks decreased levels of fasting blood glucose, plasma insulin, triglyceride, total cholesterol, and blood glycosylated hemoglobin; conversely, levels of glucagon-like peptide-1 and high-density lipoprotein-cholesterol showed an increase in the same treatment. In addition, weights of mesenteric, epididymal, and total adipose tissues decreased with DNJR supplementation, when compared with diabetic control db/db mice, while maltase, lactase, and sucrase activity in the small intestine were inhibited. The anti-diabetic effects were marginally greater in the DM-DNJR group than in the DM-R group. CONCLUSIONS: These results suggest that rice coated with ML rich in DNJ can reduce hyperglycemia and hyperlipidemia in db/db mice, and may prove useful for individuals with diabetes.

Studies on Nutrio-physiological Response of Rice Plant to Root Environment (근부환경(根部環境)에 따른 수도(水稻)의 영양생리적(營養生理的) 반응(反應)에 관(關)한 연구(硏究))

  • Park, J.K.;Kim, Y.S.;Oh, W.K.;Park, H.;Yazawa, F.
    • Korean Journal of Soil Science and Fertilizer
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    • v.2 no.1
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    • pp.53-68
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    • 1969
  • The nutriophysiological response of rice plant to root environment was investigated with eye observation of root development and rhizosphere in situation. The results may be summarized as follows: 1) The quick decomposition of organic matter, added in low yield soil, caused that the origainal organic matter content was reached very quickly, in spite of it low value. In high yield soil the reverse was seen. 2) In low yield soil root development, root activity and T/R value were very low, whereas addition of organic matter lowered them still wore. This might be contributed to gas bubbles around the root by the decomposition of organic matter. 3) Varietal difference in the response to root environment was clear. Suwon 82 was more susceptible to growth-inhibitine conditions on low-yield soil than Norin 25. 4) Potassium uptake was mostly hindered by organic matter, while some factors in soil hindered mostly posphorus uptake. When the organic matter was added to such soil, the effect of them resulted in multiple interaction. 5) The root activity showed a correlation coeffieient of 0.839, 0.834 and 0.948 at 1% level with the number of root, yield of aerial part and root yield, respectively. At 5% level the root-activity showed correlation-coefficient of 0.751, 0.670 and 0.769 with the uptake of the aerial part of respectively. N, P and K and a correlation-coefficient of 0.729, 0.742 and 0.815 with the uptake of the root of respectively N.P. and K. So especially for K-uptake a high correlation with the root-activity was found. 6) The nitrogen content of the roots in low-yield soil was higher than in high-yield soil, while the content in the upper part showed the reverse. It may suggest ammonium toxicity in the root. In low-yield soil Potassium and Phosphorus content was low in both the root and aerial part, and in the latter particularly in the culm and leaf sheath. 7) The content of reducing sugar, non-recuding sugar, starh and eugar, total carbohydrates in the aerial part of plants in low yield soil was higher than in high yield soil. The content of them, especially of reducing sugar in the roots was lower. It may be caused by abnormal metabolic consumption of sugar in the root. 8) Sulfur content was very high in the aerial part, especially in leaf blade of plants on low yield soil and $P_2O_5/S$ value of the leaf blade was one fifth of that in high yield soil. It suggests a possible toxic effect of sulfate ion on photophosphorization. 9) The high value of $Fe/P_2O_5$ of the aerial part of plants in low yield soil suggests the possible formation of solid $Fe/PO_4$ as a mechanical hindrance for the translocation of nutrients. 10) Translocation of nutrients in the plant was very poor and most nutrients were accumulated in the root in low yield soil. That might contributed to the lack of energy sources and mechanical hindrance. 11) The amount of roots in high yield soil, was greater than that in low yield soil. The in high-yield soil was deep, distribution of the roots whereas in the low-yield soil the root-distribution was mainly in the top-layer. Without application of Nitrogen fertilizer the roots were mainly distributed in the upper 7cm. of topsoil. With 120 kg N/ha. root were more concentrated in the layer between 7cm. and 14cm. depth. The amount of roots increased with the amount of fertilizer applied.

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Aboveground Biomass, N and P Distribution, and Litterfall in Pinus rigida and Larix leptolepis Plantations (리기다소나무와 낙엽송(落葉松) 인공조림지(人工造林地)의 지상부(地上部) 생체량(生體量), 질소(窒素)와 인(燐)의 분포(分布) 및 낙엽(落葉)에 관한 연구(硏究))

  • Kim, Jong-Sung;Son, Yowhan;Lim, Joo-Hoon;Kim, Zin-Suh
    • Journal of Korean Society of Forest Science
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    • v.85 no.3
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    • pp.416-425
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    • 1996
  • Aboveground biomass, distribution of N and P in tree components, forest floor and the top 20cm of mineral soil, and litterfall were determined in adjacent 37-year-old plantations of Pinus rigida Mill. and Larix leptolepis Gord. on a sandy clay loam soil in Yangpyeong, Kyonggi Province. Total above-ground tree biomass for P. rigida and L. leptolepis were 138.2 and 127.2 t/ha, respectively, and did not differ between the two species. Nitrogen concentrations in stembark and foliage were all greater in L. leptolepis. Understory biomass contributed less than 2.0% of the total aboveground biomass but contributed up to 12.0% of the aboveground nutrient contents. Soil below L. leptolepis had higher concentrations of N and P. Total ecosystem N content (kg/ha) was greater for L. leptolepis (5,579) than P. rigida (4,147). Litterfall dry mass (kg/ha/yr) was greater for P. rigida (6,020) than L. leptolepis (4,191) whereas N contents in leaf litterfall (kg/ha/yr) was almost twice as large in L. leptolepis (28) than P. rigida (16). This common garden experiment suggests a strong influence of tree species on nutrient distribution and cycling in a forest ecosystem.

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Shattering Grain Loss in Relation to the Impulse During Harvest and Year-Variation of Grain Tensile Strength in Rice (벼 수확시 충격에 의한 탈립 손실과 탈립성의 년차간 변이)

  • Yong Woong, Kwon;Jin Chul, Shin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.4
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    • pp.419-424
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    • 1983
  • Tensile strength and shattering losses of rice grains in a binder harvest and in a free falling test were examined. Indica$\times$Japonica varieties, Milyang 23, Hangangchal, and Taebaek, and a Japonica variety, Jinheung were used for this study. The average tensile strength varied with varieties, showing 0.743N (Newton) in Milyang 23, 1.078N in Hangangchal, 1.573 N in Taebaek, and 1.775 N in Jinheung. Extrapolation of the average tensile strength for the zero field loss of grain indicated about 1.7 N. Moisture content of straw at the cutting position in a binder harvest was 76.0 to 86.7% in culm and 76.7 to 77.8% in leaf sheath. The percent loss of grains occurring in binder barvest in the field was equivalent to the degree of shattering in the panicle-falling test onto the concrete floor at the height of about 40cm. The grain shattering (Y) was closely related to the impulse (X), the change in momentum: Y=-25.73+4958.4X, ($r^2$=0.987) for Milyang 23, Y=-12.97+2258.3X, ($r^2$=0.979) for Hanagangchal, Y=-5.24+840.4X, ($r^2$ =0.976) for Taebaek. The tensile strengths of grains and their variance varied singnificantly yearly even in the same variety. The magnitude of year-variance was somewhat smaller for the easily shattering variety.

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Free Radical Scavenging, Cytotoxic Effects, and Flavonoid Content of Fractions from Leaves of Lycopus lucidus Turcz. (택란 잎 추출물의 라디칼 소거활성과 세포독성효과 및 플라보노이드 함량)

  • Na, Eun;Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.29 no.3
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    • pp.337-344
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    • 2019
  • The free radical scavenging, cytotoxic effects, and flavonoid content of fractions from Lycopus lucidus Turcz leaves were here investigated. The flavonoid contents of 85% methanol (MeOH) and n-butanol (BuOH) fractions of the leaves were 41.5 mg/g and 77.2 mg/g, respectively. In DPPH and ABTs+ assays, 85% MeOH and n-BuOH fractions from the L. lucidus Turcz leaves had a greater scavenging effect (p<0.05). The n-BuOH fraction (0.5 mg/ml concentration) had scavenging effects of 88% and 92% in the DPPH and ABTs+ assays, respectively (p<0.05). Cell viability tests showed that treatment with L. lucidus Turcz leaf fractions caused cytotoxicity in the growth of AGS, HT-29, and HT-1080 cancer cells. Of the different fractions, the 85% MeOH sample displayed the highest cytotoxic activity; the $IC_{50}$ values of this fraction against AGS, HT-1080, and HT-29 cancer cells were 0.03 mg/ml, 0.14 mg/ml, and 0.16 mg/ml, respectively. These biological results indicate that the n-BuOH fraction was more effective in anti-oxidant activity while the 85% MeOH fraction was stronger in cytotoxic effects, and they suggest that these two fractions from L. lucidus Turcz leaves may contain valuable bioactive compounds, such as flavonoids.

Effect of Young Barley Leaf on Lipid Contents and Hepatic Lipid-Regulating Enzyme Activities in Mice Fed High-Fat Diet (보리순이 고지방을 급여한 마우스의 지질 함량과 간조직의 지질대사 관련 효소활성에 미치는 영향)

  • Yang, Eun-Ju;Cho, Young-Sook;Choi, Myung-Sook;Woo, Myoung-Nam;Kim, Myung-Joo;Shon, Mi-Yae;Lee, Mi-Kyung
    • Journal of Nutrition and Health
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    • v.42 no.1
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    • pp.14-22
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    • 2009
  • This study was conducted to investigate the effects of powdered young barley leaf and its water extract on body weight and lipid metabolism in high-fat fed mice. Male mice were divided into normal group, high-fat (HF) group, high-fat group supplemented with powdered young barley leaf (HF-YBL) and high-fat group supplemented with water extract of the powdered young barley leaf (HF-WYBL). The powdered young barley leaf or its water extract was added to a standard diet based on 1% dried young barley leaf (1 g YBL/100 diet and 0.28 g WYBL/100 g diet) for 8 weeks. Supplementation of YBL and WYBL significantly reduced body weight and epididymal adipose tissue weight in high-fat fed mice. Food intake and daily energy intake were significantly lower in the YBL group than in the HF group. After 8 weeks, plasma triglyceride and cholesterol concentrations were significantly higher in the HF group than in the Normal group; however, both YBL and WYBL significantly lowered those of the high-fat fed mice. The ratio of HDL-cholesterol/total cholesterol of the YBL and WYBL groups were significantly elevated compared to that of HF group. Both YBL and WYBL significantly increased fecal excretion of triglyceride in high-fat fed mice, whereas they did not affect fecal cholesterol concentration. The triglyceride levels of liver, adipose tissue and heart were significantly lower in the YBL and WYBL groups than in the HF group. Supplementation of WYBL also lowered the kidney triglyceride and heart cholesterol concentrations compared to those of HF group. Hepatic lipid regulating enzyme activities, fatty acid synthase, HMG-CoA reductase and acyl-coenzyme A: cholesterol acyltransferase, were significantly lower in the YBL and WYBL groups than in the HF group. Accordingly, these results suggest that YBL and WYBL improve plasma and organ lipid levels partly by increasing fecal lipid excretion and inhibiting fatty acid and cholesterol biosynthesis in the liver.

Physiological studies on the sudden wilting of JAPONICA/INDICA crossed rice varieties in Korea -I. The effects of plant nutritional status on the occurrence of sudden wilting (일(日). 인원연교잡(印遠緣交雜) 수도품종(水稻品種)의 급성위조증상(急性萎凋症狀) 발생(發生)에 관(關)한 영양생리학적(營養生理學的) 연구(硏究) -I. 수도(水稻)의 영양상태(營養狀態)가 급성위조증상(急性萎凋症狀) 발생(發生)에 미치는 영향(影響))

  • Kim, Yoo-Seob
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.3
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    • pp.316-338
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    • 1988
  • To identify the physiological phenomena on the sudden wilting of japonica/indica crossed varieties, Pot experiment was carried out under the heavy N application with various levels of potassium in Japan. The results obtained are as follows. 1. Sudden wilting was occurred in both varieties used, Yushin and Milyang 23. The former showed a higher degree than the latter. 2. Sudden wilting was occurred into two types, one at early ripening stage and the other at late ripening stage. The former type was found in the field with low potassium supply and the latter was seemed to be related to varietal wilting tolerence. 3. By the investigation of concerning the effective tillering rate and the change of dry weight of each organ at the heading stage, it was inferred that the growth status from young panicle formation stage to heading stage were related to sudden wilting tolerence. 4. Manganese content at heading stage, ratio of Fe/Mn and Fe. Fe/Mn in stern at late ripening stage and $K_2$ O/N ratio of stem at harvesting stage were recognized as the specific factors in connection with sudden wilting. Mn content in the sudden wilting rice plant was already in creased remarkably at heading stage. In relation to root age and absoption characteristics of Mn, the senility of root before heading stage was inferred as the cause of increase the value of Fe/Mn or Fe. Fe/Mn. 5. The $K_2$ O/N ratio of culm at harvesting stage was lower in upper node than lower node in relation to sudden wilting. And it was well accordance with the fact that the symptoms of sudden wilting proceeded from upper leaf to lower leaf. These phenomenon was different from the usual one that the effect of potassium deficiency was more remarkable in lower node than upper node. 6. All varieties which have a condition of potassium deficiency have a high degree of nitrogen content of leaves at heading stage and the $K_2$ O/N ratio of each organ was low, Especialy, $K_2$ O/N ratio is much lower in sheath and culm than leaves.

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Studies on Salt Injuries of Seedlings Growth-Sand Media Effect of Various Concentration of Added Salts upon 3 Kinds of Seedlings (수묘(樹苗)의 염해(鹽害)에 관(關)한 연구(硏究) -Nacl 농도(濃度)를 달리한 사경(砂耕)에서의 몇 수묘(樹苗)에 미치는 영향(影響))

  • Son, Won Ha
    • Journal of Korean Society of Forest Science
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    • v.8 no.1
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    • pp.3-10
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    • 1968
  • (1) The present work is a study on the tolerance of salt concentration, salt injuries and growth, under varied conditions, of salt concentration of five kind silvicultural species. (2) Pinus thunbergii, which is tolerant of stat, has shown both low fresh leaf's pH and low gravity, while Pinus densiflora, Abies bolophylla and Pinus koraiensis have respectively shown high fresh leaf's pH and high gravity. (3) Pinus thunbergii, which is tolerant otosalt, has shown high water content ind its lead, hbile such speciesras Pinus densiflora an Abies lophylla, which are intolerant of salt have shownlow water content in their leaves. (4) A signifficance has ibeen found, in the nonreatment fresh les's pH and n gravity, among Pinusthunbergiis Pinu rigida, Pinus koraiensis, Pinus densiflora and Abies holophya. (5) The order of thetlolerance of salt among the tested species is as foohws : Pinus thunbergii, P. Korea iensis, Abies holopylla. (6) The signs of salt injuries have been observed at the tops of the trees 2~3 hours after the application of 1.5% salt solution in the case of Abiihs holop hylla which are intolerant of salt, while they have been of served at the same position 4~6 hours after the 1.5% application in the case of Pinus Koraiensis and Pinus thunbergii.

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