• Title/Summary/Keyword: Leaf Growth

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Growth Promoting Rhizospheric and Endophytic Bacteria from Curcuma longa L. as Biocontrol Agents against Rhizome Rot and Leaf Blight Diseases

  • Vinayarani, G.;Prakash, H.S.
    • The Plant Pathology Journal
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    • v.34 no.3
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    • pp.218-235
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    • 2018
  • Plant growth promoting rhizobacteria and endophytic bacteria were isolated from different varieties of turmeric (Curcuma longa L.) from South India. Totally 50 strains representing, 30 PGPR and 20 endophytic bacteria were identified based on biochemical assays and 16S rDNA sequence analysis. The isolates were screened for antagonistic activity against Pythium aphanidermatum (Edson) Fitzp., and Rhizoctonia solani Kuhn., causing rhizome rot and leaf blight diseases in turmeric, by dual culture and liquid culture assays. Results revealed that only five isolates of PGPR and four endophytic bacteria showed more than 70% suppression of test pathogens in both assays. The SEM studies of interaction zone showed significant ultrastructural changes of the hyphae like shriveling, breakage and desication of the pathogens by PGPR B. cereus (RBacDOB-S24) and endophyte P. aeruginosa (BacDOB-E19). Selected isolates showed multiple Plant growth promoting traits. The rhizome bacterization followed by soil application of B. cereus (RBacDOB-S24) showed lowest Percent Disease Incidence (PDI) of rhizome rot and leaf blight, 16.4% and 15.5% respectively. Similarly, P. aeruginosa (BacDOB-E19) recorded PDI of rhizome rot (17.5%) and leaf blight (17.7%). The treatment of these promising isolates exhibited significant increase in plant height and fresh rhizome yield/plant in comparison with untreated control under greenhouse condition. Thereby, these isolates can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.

Effect of Different Light Intensities on the Growth and Leaf Gas Exchanges in Miscanthus sinensis and Pennisetum purpurascens (참억새 및 수크령의 광도차에 따른 생육변화 및 가스교환에 미치는 영향)

  • Kwack, Hye Ran;Lee, Jong Suk
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.1
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    • pp.110-115
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    • 2004
  • This experiment was conducted to investigate the effect of light intensities on the growth responses, carbohydrate contents and the characteristics of leaf gas exchange in Miscanthus sinensis and Pennisetum purpurascens. The plant height and leaf length were increased to about 30% in the sun. However, those were reduced severely in the shade, and leaf necrosis was also observed. The representative growth index and the dry weight of 2 species were 50% higher than shade and the rate was reduced according to the decrease of light intensities. Total carbohydrate contents showed very similar changes to that of dry weight. However, any notable influences were observed at above the light intensities of 250~500${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in the half shade. The size of spikes and the earliest spiking appeared in the sun and the spike color was decolorized as decreased in light intensities, irrespective of species. Photosynthetic rate of 2 species was 2 times higher in the sun than those in the shade, and it showed the typical photoresponses of sun plant. Stomatal conductances and intercelluar $CO_2$ concetration showed similar changes to that of photosynthetic rate. On the contrary, vapor pressure deficit was increased more in the shade than in the sun.

Anticancer Effect of Bottled Mustard Leaf Kimchi during Fermentation (병 포장 갓김치의 항암성에 대한 연구)

  • Kim, Bog-Nam
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.6
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    • pp.1063-1071
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    • 2008
  • Mustard Leaf Kimchi (MLK) is a traditional fermented Korean vegetable food. This study investigated the anticancer effect of partial vacuum treatment of MLK packed in glass bottles during fermentation. Prepared vacuum treated mustard leaf Kimchi (VM) and non-vacuum treated mustard leaf Kimchi (CM) were fermented at $5^{\circ}C$ for 8 weeks. The initial pH and total acidity were approximately 5.7 and, 0.36%, respectively. During fermentation, pH decreased and total acidity increased. Initial contents of reducing sugar and salt were 2.1% and were 2.7 mg/g, respectively. Reducing sugar gradually decreased during fermentation. Growth of cells from mouse cancer cell lines (L12l0 and P338D1) and human cancer cell lines (HepG2 and WiDr) were all decreased by MLK. VM and CM did not affect growth. More potent growth inhibition effects were exhibited by water versus hexane extracts of MLK, and by MLK fermented for 3 weeks versus 6 weeks. However, when applied to control NIH/3T3 cells at the same concentrations, MLK exhibited no cytotoxicity, and cell growth was unimpeded.

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Effects of different day length and wind conditions to the seedling growth performance of Phragmites australis

  • Hong, Mun Gi;Nam, Bo Eun;Kim, Jae Geun
    • Journal of Ecology and Environment
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    • v.45 no.2
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    • pp.78-87
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    • 2021
  • Background: To understand shade and wind effects on seedling traits of common reed (Phragmites australis), we conducted a mesocosm experiment manipulating day length (10 h daytime a day as open canopy conditions or 6 h daytime a day as partially closed canopy conditions) and wind speed (0 m/s as windless conditions or 4 m/s as windy conditions). Results: Most values of functional traits of leaf blades, culms, and biomass production of P. australis were higher under long day length. In particular, we found sole positive effects of long day length in several functional traits such as internode and leaf blade lengths and the values of above-ground dry weight (DW), rhizome DW, and total DW. Wind-induced effects on functional traits were different depending on functional traits. Wind contributed to relatively low values of chlorophyll contents, angles between leaf blades, mean culm height, and maximum culm height. In contrast, wind contributed to relatively high values of culm density and below-ground DW. Conclusions: Although wind appeared to inhibit the vertical growth of P. australis through physiological and morphological changes in leaf blades, it seemed that P. australis might compensate the inhibited vertical growth with increased horizontal growth such as more numerous culms, indicating a highly adaptive characteristic of P. australis in terms of phenotypic plasticity under windy environments.

Leaf Characteristics and Growth Performance in Progenies of Hovenia dulcis var. koreana Nakai (헛개나무 선발개체 차대의 생장 및 엽특성)

  • Kim, Sea-Hyun;Chung, Hun-Gwan;Jang, Yong-Seok;Han, Jin-Gyu
    • Korean Journal of Plant Resources
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    • v.20 no.1
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    • pp.7-11
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    • 2007
  • This study was conducted by estimate of leaf characteristics and growth performance among selected 20 families progenies of Hovenia dulcis var. koreana Nakai. Mean seedling height and root-collar diameter in whole progenies showed 61.6cm, 6.98mm, respectively. Mean seedling height of KW 2-5 family was 79.3cm and it was higher than that of other families. JN 2-2 family had highest value, 9.16mm, in mean of root-collar diameter. Growth performances of KW 2-5 and JN 2-2 families showed 57% (in seedling height), 53% (in root-collar diameter) superior to those of lowest marked families. The selection level based on growth performances, which are seedling height over 75.0cm and root-collar diameter over 8.0mm, were applied on whole families, and two families selected (KW 2-5, JN 2-2). The selection effects from selected families was evaluated as 125% compared to the mean of whole families. The coefficient of variation (C.V) appeared wide range, $9.4{\sim}42.4%$, in estimated leaf characteristics. Particularly, C.V of petiole length (PL) and petiole diameter (PD) showed 42.4% and 28.7%, respectively. Their were higher value than other leaf characteristics. Analysis of variance for all leaf characteristics were significantly different among families. Also, there had positive correlation between most leaf characteristics except the correlation between leaf length (LL) and leaf morphological index (LMI, LW/LL). Particularly, the correlation between leaf length and leaf width (LW) showed the highest correlation.

Nitrogen Management with Split Application of Urea for Direct-Seeding Rice in Wet Paddy

  • Lee, Ho-Jin;Seo, Jun-Han;Lee, Jung-Sam;Jung, Yong-Sang;Fred E. Below
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.1
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    • pp.49-53
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    • 1998
  • Direct-seeding has major advantages such as labor and cost saving by eliminating preparation of seed bed and transplanting. But, it required increased input of fertilizers and pesticides because of the extended paddy period. Direct seeding in wet paddy (DSWP) gives faster growth and more uniform seedling emergence than direct-seeding in dry paddy. This research had an objective to develop an efficient N management practices for DSWP with split application of N fertilizer. A paddy field experiment was conducted to evaluate effects of starter N and N-topdressing which was delayed N application until 5-leaf stage, with comparison to transplanting (TP). Total amount of N application were two levels; 110kg and 77kg/ha. The N applications were split four times during rice growth stages; starter, topdressing at 5-leaf stage, top dressing at tillering stage, and topdressing at panicle initiation stage. DSWP had more tillers/$m^2$ than TP, but with the delayed heading. The DSWP plots which received N-topdressing at 5-leaf stage without starter N had higher leaf area index (LAI) and leaf greenness than the TP plot. Also, these DSWP plots had high leaf-N concentration at the heading stage, as calculated from leaf chlorophyll meter readings. Rice yield in DSWP with N-topdressing at 5-leaf stage was significantly higher than that in TP and in DSWP with starter N. Energy and N use efficiency were improved in DSWP with N-topdressing at 5-leaf stage. But, there were no significant differences in grain yield between the two levels of total amounts of N applications, 77kg and 110kg/ha. We concluded that starter N could not be used effectively by rice seedlings, but topdressing N at 5-leaf stage was an efficient N management for rice growth and yield in DSWP system.

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Linking Leaf Functional Traits with Plant Resource Utilization Strategy in an Evergreen Scrub Species Rhododendron caucasicum Pall. along Longitudinal Gradient in Georgia (The South Caucasus)

  • Ekhvaia, Jana;Bakhia, Arsena;Asanidze, Zezva;Beltadze, Tornike;Abdaladze, Otar
    • Journal of Forest and Environmental Science
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    • v.38 no.2
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    • pp.110-121
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    • 2022
  • Leaf functional traits widely have been used to understand the environmental controls of resource utilization strategy of plants along the environmental gradients. By using key leaf functional traits, we quantified the relationships between leaf traits and local climate throughout the distributional range of Rhododendron caucasicum Pall. in eastern and western Georgian mountains (the South Caucasus). Our results revealed, that all traits showed high levels of intraspecific variability across study locations and confirmed a strong phenotypic differentiation of leaf functional variation along the east-west longitudinal gradient in response to the local climate; out of the explored climatic variables, the moisture factors related to precipitation and number of precipitation and dry days for winter and growth seasons were more strongly related to leaf trait variation than the elevation and air temperature. Among studied leaf traits, the leaf specific area (SLA) showed the highest level of variability indicating the different resource utilization strategies of eastern and western-central Rh. caucasicum individuals. High SLA leaves for western-central Caucasian individuals work in relatively resource-rich environments (more humid in terms of precipitation amount and the number of precipitation days in winter) and could be explained by preferential allocation to photosynthesis and growth, while eastern Caucasian samples work in resource-poor environments (less humid in terms of precipitation amount and the number of precipitation days in winter) and the retention of captured resources is a higher priority appearing in a low SLA leaves. However, more evidence from a broader study of the species throughout its distribution range by including additional environmental factors and molecular markers are needed for firmer conclusions of intraspecific variability of Rh. caucasicum.

Influence of basal medium formulations and silver nanoparticles on in vitro plant growth in gerbera

  • Hyun Hee Kang;Aung Htay Naing;Junping Xu;Mi Young Chung;Su Young Lee;Jeung-Sul Han;Chang Kil Kim
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.183-189
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    • 2023
  • This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

Effect of Microorganism, Vitabio on Growth and Quality of Leaf Lettuce (Vitabio 土壤微生物劑 處理가 葉상치 收量 및 品贊에 미치는 영향)

  • Kim, Kyung-Je;Lee, Byung-Moo
    • Korean Journal of Organic Agriculture
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    • v.12 no.3
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    • pp.345-352
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    • 2004
  • This study was carried out to investigate the effect of soil-born microorganism, vitabio on growth of leaf lettuce in the vinyl house. Total weights of leaf lettuce treated with vitabio showed difference compared with untreated leaf lettuce. Sugar content was also increased. Mineral contents of leaf lettuce showed no difference between treated with vitabio and untreated with vitabio. Exchange Capacity (EC) and Organic Matter (OM) in chemical properties of soil treated with vitabio showed higher than soil in untreated vitabio. Vitabio treated soil contained much more microorganisms such as Bacteria, Actinomycetes, Hyphomycetes, Bacillus sp. Pseudomonas sp. after harvest.

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Effect of Physical Properties of Soil on Ginseng Seedling Growth in Nursery Bed (양식묘단 토양의 물리성이 묘삼생육 및 수량에 미치는 영향)

  • 이종철;변정수
    • Journal of Ginseng Research
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    • v.19 no.3
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    • pp.287-290
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    • 1995
  • This study was conducted to elucidate the effect of physical properties of soil in nursery bed with different densities on growth of ginseng seedling. Stem length, leaf length and leaf width of ginseng seedling showed the decreasing tendency with increasing the hardness of the nursery soil. Fresh root weight per seedling and number of available seedlings were increased significantly with decrease of the soil hardness. For solid, liquid phases, bulk density and hardness of soil, negative correlations were shown in stem length, leaf length, leaf width, root weight per seeding, and number t of available seedlings. On the other hand, gas phase, air permeability and porosity of soil had positive correlations with stem length, leaf length, leaf width, root weight per seedling and number of available seedlings. Key words Yang-Jik nursery, ginseng seedling, soil physical properties.

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