• Title/Summary/Keyword: Layer-specific

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Targeted Protein Expression in Freshly Isolated Vascular Tissues by Using Adenoviral Vector (생체에서 분리된 혈관조직에서 아데노바이러스벡터를 이용한 특정 단백질의 발현)

  • Huh, Yang Hoon;Kim, Hak Rim
    • YAKHAK HOEJI
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    • v.57 no.4
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    • pp.265-271
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    • 2013
  • Treatments of vascular disease via modulating the expression of specific proteins by gene transfer have been attempted in various studies over the past few years. Among several methods to deliver genes, adenovirus currently has been used because of a number of positive aspects. In this study, we test adenoviral vector as a potential mediator in the treatment of vascular disease by using freshly isolated vascular tissues not cultured vascular cells. Freshly isolated vascular tissues were directly exposed to adenoviral vector pAd5CMVmcsIRESeGFPpA to check the possibility of GFP expression in different layer of vascular tissues. We found that the GFP expression by using adenoviral vector experiments is mainly focused on the adventitia and failed to detect GFP expression at endothelial layer or vascular smooth muscle layer in vascular tissues. However, we also found that several integrin receptors are robustly expressed in vascular smooth muscle, thus the limited expression of protein in vascular smooth muscle are not likely the lack of integrin receptors. In conclusion, adenovirus could not be a good tool for a specific protein expression in vascular smooth muscle cell. Thus, the application of adenovirus as a tool for gene therapy of vascular smooth muscle cells in clinical therapeutic trial need to be optimized further.

Characterization of Electric Double-Layer Capacitors with Carbon Nanotubes Directly Synthesized on a Copper Plate as a Current Collector (구리 집전판에 직접 합성한 탄소나노튜브의 전기이중층 커패시터 특성)

  • Jung, Dong-Won;Lee, Chang-Soo;Park, Soon;Oh, Eun-Souk
    • Korean Journal of Metals and Materials
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    • v.49 no.5
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    • pp.419-424
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    • 2011
  • Carbon nanotubes (CNTs) were directly synthesized on a copper (Cu) plate as a current collector by the catalytic thermal vapor deposition method for an electric double-layer capacitor (EDLC) electrode. The diameters of vertically aligned CNTs grown on the Cu plate were 20~30 nm. From cyclic voltammetry (CV) results, the CNTs/Cu electrode showed high specific capacitance with typical profiles of EDLCs. Rectangularshaped CV curves suggested that the CNTs/Cu electrode could be an excellent candidate for an EDLC electrode. The specific capacitances were in a range of 25~75 F/g with a scan rate of 10~100 mV/s and KOH electrolyte concentration 1~6 M, and were maintained up to 1000 charge/discharge cycles due to strong adhesion between the Cu substrate and the CNTs.

Analysis of electrochemical double-layer capacitors using a Natural Rubber-Zn based polymer electrolyte

  • Nanditha Rajapaksha;Kumudu S. Perera;Kamal P. Vidanapathirana
    • Advances in Energy Research
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    • v.8 no.1
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    • pp.41-57
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    • 2022
  • Electrochemical double-layer capacitors (EDLCs) based on solid polymer electrolytes (SPEs) have gained an immense recognition in the present world due to their unique properties. This study is about preparing and characterizing EDLCs using a natural rubber (NR) based SPE with natural graphite (NG) electrodes. NR electrolyte was consisted with 49% methyl grafted natural rubber (MG49) and zinc trifluoromethanesulfonate ((Zn(CF3SO3)2-ZnTF). It was characterized using electrochemical impedance spectroscopy (EIS) test, dc polarization test and linear sweep voltammetry (LSV) test. NG electrodes were made using a slurry of NG and acetone. EIS test, cyclic voltammetry (CV) test and galvanostatic charge discharge (GCD) test have been done to characterize the EDLC. Optimized electrolyte composition with NR: 0.6 ZnTF (weight basis) exhibited a conductivity of 0.6 x 10-4 Scm-1 at room temperature. Conductivity was predominantly due to ions. The electrochemical stability window was found to be from 0.25 V to 2.500 V. Electrolyte was sandwiched between two identical NG electrodes to fabricate an EDLC. Single electrode specific capacitance was about 2.26 Fg-1 whereas the single electrode discharge capacitance was about 1.17 Fg-1. The EDLC with this novel NR-ZnTF based SPE evidences its suitability to be used for different applications with further improvement.

Changes in the Specific Gravity of Pacific Cod Gadus macrocephalus, During the Early Life Stages (대구(Gadus macrocephalus)의 초기 발생시기의 비중변화)

  • Lee, Hwa Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.3
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    • pp.332-337
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    • 2018
  • The Pacific cod Gadus macrocepahlus, lays demersal eggs and the hatching larvae rise toward the surface layer of the ocean to feed. The change in the specific gravity of eggs and larvae was investigated to examine their vertical distribution and movement in the water column. The specific gravities of fertilized eggs and various size classes of larvae were measured using a density gradient apparatus. In total, the instantaneous specific gravity of 146 eggs and 225 larvae were measured. To prevent any disturbance in the gradient water column due to larval movement, 0.004% MS222 was used for anesthesia. Due to their high specific gravity, eggs spawned were deposited over the sea-bed of the spawning ground. The specific gravity of hatching larvae decreased abruptly. However, Pacific cod larvae still had a comparatively high specific gravity at hatching ($1.03655{\pm}0.00146g/cm3$, n=4, mean SL=3.62 mm) and their specific gravities tended to decrease as they grew. The specific gravity stabilized 6 days after hatching ($1.02590{\pm}0.00212g/cm3$, n=15, mean SL=4.67 mm) and the cod larvae were eventually able to float in the water column.

Preparation and Characterization of Carbon Nanotubes-Based Composite Electrodes for Electric Double Layer Capacitors

  • Seo, Min-Kang;Park, Soo-Jin
    • Bulletin of the Korean Chemical Society
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    • v.33 no.5
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    • pp.1523-1526
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    • 2012
  • In this work, we prepared activated multi-walled carbon nanotubes/polyacrylonitrile (A-MWCNTs/C) composites by film casting and activation method. Electrochemical properties of the composites were investigated in terms of serving as MWCNTs-based electrode materials for electric double layer capacitors (EDLCs). As a result, the A-MWCNTs/C composites had much higher BET specific surface area, and pore volume, and lower volume ratio of micropores than those of pristine MWCNTs/PAN ones. Furthermore, some functional groups were added on the surface of the A-MWCNTs/C composites. The specific capacitance of the A-MWCNTs/C composites was more than 4.5 times that of the pristine ones at 0.1 V discharging voltage owing to the changes of the structure and surface characteristics of the MWCNTs by activation process.

Degumming Characteristics and Color Stability of GoldenSilk Cocoon

  • Kweon, Hae-Yong;Lee, Kwang-Gill;Park, Kwang-Young;Kang, Seok-Woo;Seok, Young-Seek
    • International Journal of Industrial Entomology and Biomaterials
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    • v.24 no.1
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    • pp.1-5
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    • 2012
  • A newly developed silkworm variety, GoldenSilk makes bright yellow cocoon. In this article, the basic characteristics of GoldenSilk cocoon were examined through color analysis, degumming characteristics and UV spectrophotometer. The colorant of GoldenSilk cocoon was not distributed evenly throughout the cocoon layer but existed in the outer layer of the cocoon. The colorant has not good resistance against sunlight. The degumming loss percentage of GoldenSilk cocoon was 29.2%, which is relatively higher than that of the authorized one. UV spectrophotometer showed specific absorption band around 400 to 500 nm, which is the specific yellow colorant of the GoldenSilk cocoon extracted to the degumming solution during the degumming process.

Effect of Conducting Composite on Characteristics of Electric Double Layer Capacitor (전기이중층 캐패시터의 특성에 미치는 혼성 도전재의 영향)

  • Kim, Ick-Jun;Lee, Sun-Young;Do, Chil-Hoon;Moon, Seong-In;Choi, Sung-Ok;Son, Young-Mo;Kim, Kyung-Ho
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.07b
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    • pp.1140-1143
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    • 2002
  • This work describes the effect of conducting composite on the characteristics of electric double layer capacitor. The cell, which was fabricated with conducting composite consisted of 50 wt.% of SPB and 50 wt.% of VGCF, exhibits the higher specific capacitance, the lower resistance and the better rate capability than those of the cells fabricated with each single electronic conductor. These enhanced properties could be related with the dense structure of electrode.

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Laser cladding of Ni-base superalloy on low carbon steel (저탄소강에 대한 Ni기 초합금의 레이저 클래딩)

  • 이제훈;서무홍;김정오;한유희
    • Laser Solutions
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    • v.2 no.2
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    • pp.34-41
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    • 1999
  • A RS840 $CO_2$laser and a powder auto-feeding apparatus have been used to deposit single tracks of Ni-base superalloy on low carbon steel. In this paper, the effects of laser cladding parameters on clad geometry, dilution and microhardness are studied. As a results, the w/h ratio of the clad layer increases with decreasing powder feed rate and increasing laser scan speed. Increase of powder density and decrease of specific energy have little effect on dilution. It was found that the clad layer of the highest hardness has a structure in which fine and leaf like phases are dispersed in ${\gamma}$Ni matrix.

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Electrochemical Characteristics of Highly Porous Carbon Prepared by Chemical Activation Method for EDLC (화학적 활성법으로 제조된 EDLC용 고다공성 탄소전극의 전기화학 특성)

  • Eo, Soo-Mi;Kim, Han-Joo;Oh, Seung-Mo;Park, Soo-Gil
    • Proceedings of the KIEE Conference
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    • 2005.07c
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    • pp.2010-2012
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    • 2005
  • Activated carbon was activated with chemical treatment to attain high surface area with porous structure. We have been considered activated carbon is the ideal material for high voltage electric double layer capacitor due to their high specific surface area, good conductivity and chemical stability. In this study we found that increase in electrochemical capacitance due to activated carbon. Also chemically activated carbon and water treatment have resulted larger capacitance and also exhibits better electrochemical behavior, and is about 15% more than in untreated state. The structural change in activated carbon through chemical treatment activation was investigated by using SEM and XRD. In this study, the dependence of the activation behavior with KOH in the micro structure of host materials will be discussed. Furthermore, the relation to the electric double layer capacitance, especially the specific capacitance per unit area, is also discussed.

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Effects of Emulsifiers on the Properties of White Layer Cakes Prepared from Geurumil Flour (그루밀가루의 White Layer Cake 적성과 유화제 첨가 효과)

  • 경문식;장학길;이영택
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.877-881
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    • 2001
  • A domestic wheat variety (Geurumil flour) was evaluated for the properties of white layer cake, and compared to a commercial soft wheat flour. Geurumil flour contained more protein, ash, and lipid contents than commercial soft wheat flour. Very little difference in cake batter pH was observed between soft wheat flour and Geurumil flour, and the addition of emulsifiers tended to decrease the pH of Geurumil cake batter The specific gravity of the cake batter was lower in Geurumil flour than in soft wheat flour, and decreased effectively by the addition of emulsifiers due to batter aeration. Specific loaf volume was influenced by the addition of emulsifiers and demonstrated the highest values at the level of 1 ~ 2%. Addition of Ester-400 (monoglyceride) showed better cake properties in terms of volume, symmetry and uniformity index than sucrose-fatty acid ester did, and thus appeared to be more effective in improving baking performance. The changes in firmness of cakes during storage at $25^{\circ}C$ were observed, and Ester-400 showed some Positive effects on retarding cake staling.

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