• Biomolecules & Therapeutics
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• v.8 no.3
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• pp.269-275
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• 2000

Silibinin(silybin) is the active component of silymarin from Silybum marianum and has hepato-protective effect. It is water-insoluble and has low bioavailability. To improve its bioavailability, self-micro-emulsifying drug delivery system (SMEDDS) has been developed by Hanmi Pharmaceutical Company (Silyma $n^{R}$ 140 soft capsule). In this study, the pharmacokinetic profiles of Silyma $n^{R}$ were examined and compared it with a reference preparation, L Caps140 of B Pharmaceutical Company. This study was approved by Yonsei University Severance Hospital IRB(approval No. CR0004) and followed the bioequivalence test guideline of Korean FDA. Eighteen healthy adult volunteers were allocated based on 2$\times$2 Latin square cross-over design. They were given 2 capsules (each contains silymarin 140 mg (60 mg as silibinin)) of either drug at each period and crossed over after a week of drug-free washout period. Blood concentration of silibinin was measured by HPLC. The $C_{max}$ and AUC of the Silyma $n^{R}$ were 1542.0 $\pm$ 402.7 ng/ml and 3323.3 $\pm$ 824.7 ng.h/ml, respectively, and were significantly higher than those of reference preparation. The Tmax was 0.8 $\pm$ 0.3 h and significantly shorter than reference preparation. The $K_{e}$ and $T_{1}$2/ of both drugs were comparable. Percent differences in means against reference preparation were +88.3% for AUC, +222.6% for $C_{max}$, and -61.1% for $T_{max}$./.>././.>./.

• Animal Bioscience
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• v.34 no.1
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• pp.66-73
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• 2021

Objective: Soy sauce oil, a byproduct of whole soybean processing by the soy sauce industry, was evaluated as a source of linoleic acid for dairy cows for the purpose of manipulating the composition of milk. Methods: Eight dairy Holstein cows fitted with rumen cannulas were used for ruminal administration of soy sauce oil for a 28-day period using a 4×4 Latin square study design with 4 doses (0, 200, 400, and 600 g soy sauce oil/d). Results: Although dry matter intake and milk yield were not affected by soy sauce oil administration, ruminal concentrations of total volatile fatty acids and acetate were decreased, specifically at 600 g/d administration. While milk fat percentage was decreased with administration of soy sauce oil, proportions of linoleic, vaccenic and conjugated linoleic acids in the rumen, blood and milk were increased with increasing soy sauce oil dose. Conclusion: These results suggest that soy sauce oil feeding could be useful for improving milk functionality without adverse effects on animal production performance when fed at less than 400 g/d.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.8
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• pp.1105-1116
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• 2017

Objective: A meta-analysis was conducted to evaluate the effects of supplemental fat containing saturated free fatty acids (FA) on milk performance of Holstein dairy cows. Methods: A database was developed from 21 studies published between 1991 and 2016 that included 502 dairy cows and a total of 29 to 30 comparisons between dietary treatment and control without fat supplementation. Only saturated free FA (>80% of total FA) was considered as the supplemental fat. Concentration of the supplemental fat was not higher than 3.5% of diet dry matter (DM). Dairy cows were offered total mixed ration, and fed individually. Statistical analysis was conducted using random- or mixed-effects models with Metafor package in R. Results: Sub-group analysis showed that there were no differences in studies between randomized block design and Latin square/crossover design for dry matter intake (DMI) and milk production responses to the supplemental fat (all response variables, $p{\geq}0.344$). The supplemental fat across all studies improved milk yield, milk fat concentration and yield, and milk protein yield by 1.684 kg/d (p<0.001), 0.095 percent unit (p = 0.003), 0.072 kg/d (p<0.001), and 0.036 kg/d (p<0.001), respectively, but tended to decrease milk protein concentration (mean difference = -0.022 percent unit; p = 0.063) while DMI (mean difference = 0.061 kg/d; p = 0.768) remained unchanged. The assessment of heterogeneity suggested that no substantial heterogeneity occurred among all studies for DMI and milk production responses to the supplemental fat (all response variables, $I^2{\leq}24.1%$; $p{\geq}0.166$). Conclusion: The effects of saturated free FA were quantitatively evaluated. Higher milk production and yields of milk fat and protein, with DMI remaining unchanged, indicated that saturated free FA, supplemented at ${\leq}3.5%$ dietary DM from commercially available fat sources, likely improved the efficiency of milk production. Nevertheless, more studies are needed to assess the variation of production responses to different saturated free FA, either C16:0 or C18:0 alone, or in combination with potentially optimal ratio, when supplemented in dairy cow diets.

• Journal of Animal Science and Technology
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• v.59 no.6
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• pp.15.1-15.5
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• 2017

Background: The horse intestinal tract is sensitive and contains a highly complex microbial population. A shift in the microbial population can lead to various issues such as inflammation and colic. The use of nutraceuticals in the equine industry is on the rise and curcumin is thought to possess antimicrobial properties that may help to minimize the proliferation of opportunistic bacteria. Methods: Four cecally-cannulated horses were utilized to determine the optimal dose of liposomal-curcumin (LIPC) on reducing Streptococcus bovis/equinus complex (SBEC), Escherichia coli K-12, Escherichia coli general, Clostridium difficile, and Clostridium perfringens in the equine hindgut without adversely affecting cecal characteristics. In the first study cecal fluid was collected from each horse and composited for an in vitro, 24 h batch culture to examine LIPC at four different dosages (15, 20, 25, and 30 g) in a completely randomized design. A subsequent in vivo $4{\times}4$ Latin square design study was conducted to evaluate no LIPC (control, CON) or LIPC dosed at 15, 25, and 35 g per day (dosages determined from in vitro results) for 9 days on the efficacy of LIPC on selected bacterial strains, pH, and volatile fatty acids. Each period was 14 days with 9 d for acclimation and 5 d withdrawal period. Results: In the in vitro study dosage had no effect ($P{\geq}0.42$) on Clostridium strains, but as the dose increased SBEC concentrations increased (P = 0.001). Concentrations of the E. coli strain varied with dose. In vivo, LIPC's antimicrobial properties, at 15 g, significantly decreased (P = 0.02) SBEC when compared to 25 and 35 g dosages. C. perfringens decreased linearly (P = 0.03) as LIPC dose increased. Butyrate decreased linearly (P = 0.01) as LIPC dose increased. Conclusion: Further studies should be conducted with a longer dosing period to examine the antimicrobial properties of curcumin without adversely affecting cecal characteristics.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.803-811
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• 2009

The chemical composition and water extractable dry matter (DM) of foliages from Erythrina (Erythrina variegata), Fig (Ficus racemosa), Jackfruit (Artocarpus heterophyllus), Jujube (Ziziphus jujuba Mill), Kapok (Ceiba pentandra) and Mango (Mangifera indica) and the feed intake, digestibility and N retention when feeding these foliages were studied in two experiments. In Experiment 1, 12 male goats, 3.5 months old and weighing 14.6 kg, were randomly allocated to a diet consisting of one of the foliages in a change-over design with three periods. The foliages were offered ad libitum at the level of 130% of the average daily feed intake. The Erythrina foliage had a low content of DM and condensed tannins (CT) and a high concentration of crude protein (CP) in leaves plus petioles (193 g/kg DM) and stem, while the Mango foliage had a low CP (69 g/kg DM) and high DM content. The other foliages were intermediate. High content of CT was found in the leaves plus petioles of Jackfruit foliage and in the stem of Fig and Mango foliage. There was a difference in feed intake, nutrient intake, apparent digestibility and N retention between the foliages, with Erythrina, Jackfruit and Kapok foliage being significantly higher in these parameters than Fig, Jujube and Mango foliage. The water extractable DM could be used to estimate N retention, but not DM digestibility in this study. In Experiment 2, 4 male goats weighing 13.4 kg and 6 months old were allocated to a 4${\times}$4 Latin square design. The treatments were: water spinach ad libitum and Fig, Jujube or Mango foliage ad libitum +0.5% of BW as water spinach DM. Feed intake, apparent digestibility and N retention were not significantly different among the foliage diets, but higher than for water spinach alone (p<0.05). Supplementation with water spinach to a diet consisting of low quality foliages such as Fig, Jujube and Mango, increased DM and CP intake, apparent digestibility and N retention, compared to feeding these foliages as sole feeds.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.725-732
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• 2007

The effects of supplementing Gamba grass (Andropogon gayanus) with varying levels of hay from cassava (Manihot esculenta Crantz) and dried cassava root chip on growth and diet digestibility were studied using local male goats with an average initial body weight of 14.0 kg. Thirty-two animals were allocated to a completely randomized $2{\times}2$ factorial design with eight animals per treatment. The factors were two levels of cassava hay (25% and 35% of an expected dry matter (DM) intake of 3% of body weight) and cassava root chips (0 or 1% of body weight) on an individual basis with grass offered ad libitum. Another four animals were assigned to a $4{\times}4$ Latin square design to study digestibility, and were given the same four diets as in the growth experiment. Total DM intake was significantly higher in the group fed diets with cassava hay and root while the DM intake of Gamba grass was not significantly different between treatments. The supplementation with cassava hay and root increased the apparent digestibility of DM, organic matter and N and resulted in a higher N-retention. The apparent digestibility of neutral detergent fibre and acid detergent fiber was not affected significantly. The average daily gain of animals fed diets supplemented with both cassava hay and root was significantly higher than for the animals supplemented with cassava hay alone. The highest daily gain recorded was 70 g/day. In conclusion, supplementing a basal diet of Gamba grass with cassava hay and root chips improved DM intake, digestibility, N-retention and weight gain. In order to minimize the waste of cassava hay, the inclusion level of cassava hay can be recommended to be 25% of expected DM intake, which would give acceptable intake and growth performance when cassava root is included in the diet.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.343-350
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• 2015

Cocoa pod is among the by-products of cocoa (Theobroma cacao) plantations. The aim of this study was to apply a number of treatments in order to improve nutritional quality of cocoa pod for feeding of ruminants. Cocoa pod was subjected to different treatments, i.e. C (cocoa pod without any treatment or control), CAm (cocoa pod+1.5% urea), CMo (cocoa pod+3% molasses), CRu (cocoa pod+3% rumen content) and CPh (cocoa pod+3% molasses+Phanerochaete chrysosporium inoculum). Analysis of proximate and Van Soest's fiber fraction were performed on the respective treatments. The pods were then subjected to an in vitro digestibility evaluation by incubation in rumen fluid-buffer medium, employing a randomized complete block design (n = 3 replicates). Further, an in vivo evaluation of the pods (35% inclusion level in total mixed ration) was conducted by feeding to young Holstein steers (average body weight of $145{\pm}3.6kg$) with a $5{\times}5$ latin square design arrangement (n = 5 replicates). Each experimental period lasted for 30 d; the first 20 d was for feed adaptation, the next 3 d was for sampling of rumen liquid, and the last 7 d was for measurements of digestibility and N balance. Results revealed that lignin content was reduced significantly when cocoa pod was treated with urea, molasses, rumen content or P. chrysosporium (p<0.01) with the following order of effectiveness: CPh>CAm>CRu>CMo. Among all treatments, CAm and CPh treatments significantly improved the in vitro dry matter and organic matter digestibility (p<0.05) of cocoa pod. Average daily gain of steers receiving CAm or CPh treatment was significantly higher than that of control (p<0.01) with an increase of 105% and 92%, respectively. Such higher daily gain was concomitant with higher N retention and proportion of N retention to N intake in CAm and CPh treatments than those of control (p<0.05). It can be concluded from this study that treatment with either urea or P. chrysosporium is effective in improving the nutritive value of cocoa pod.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.658-664
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• 2002

This study was conducted to determine a proper feeding regime for lactating Holstein cows during the warm season in Taiwan. In Feeding Trial, 21 lactating Holstein cows were randomly allotted into three feeding regimes in a Completely Randomized Design. The feeding regimes were roughage fed ad libitum along with concentrate fed either twice daily (2C) or four times daily (4C), and total mixed ration (TMR) for 8 weeks. No significant differences among the three feeding regimes were found in body weight changes, and intakes in terms of dry matter, crude protein and net energy. For milk yield and 4% FCM yield, 4C were higher than the other two feeding regimes (p<0.05). No significant differences were found in milk compositions or differences noted in the yields of the respective milk components. TMR was worse than 2C and 4C (p<0.05) in milk production efficiencies in terms of dry matter intake per kg milk yield, crude protein intake per kg milk protein yield, as well as dry matter intake, net energy intake and crude protein intake per kg 4% FCM yield. Three ruminally fistulated Holstein dry cows were randomly assigned into the same three feeding regimes in a 3${\times}$3 Latin Square Design. No significant differences were found among the feeding regimes in ruminal pH, ammonia nitrogen, total VFA concentration, molar percentages of VFA, and acetic acid: propionic acid ratio (C$_2$/C$_3$). Taken all together, roughage fed ad libitum and concentrate fed four times daily was the better feeding regime for lactating cows during the warm season in Taiwan.

• Journal of Pharmaceutical Investigation
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• v.28 no.4
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• pp.223-229
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• 1998

A computer program for personal computers, $K-BEtest^{\circledR}$, was developed to analyze bioequivalence data in accordance with Korean Guidelines for Bioequivalence Test (KGBT). This program is user-friendly, interactive, Hangul-compatible and supports $2{\times}2$ cross-over design as well as $2{\times}2$ Latin square design with various significance levels. This program is able to calculate AUC, $C_{max}$ and $T_{max}$ parameters from the blood drug concentration-time profile of individual subjects and evaluate the parameters statistically for the bioequivalence by ${\pm}20%$ rule, the F-test, the Non-centrality test and 90% confidence intervals. All procedures are supported with graphic interface, interactive menu and outputs in Korean. In this paper, two experimental data sets were analyzed by the program and detailed process was demonstrated. The $K-BEtest^{\circledR}$ program appears to be very effective for analyzing bioequivalence data and can be widely used with convenience and accuracy.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.39-43
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• 2018

The objective of this study was to determine the effect of various roughage sources on nutrient digestibility and enteric methane ($CH_4$), and carbon dioxide ($CO_2$) production in goats. Four castrated black goats ($48.5{\pm}0.6kg$) were individually housed in environmentally controlled respiration-metabolism chambers. The experiment design was a $4{\times}4$ balanced Latin square design with 4 roughage types and 4 periods. Alfalfa, tall fescue, rice straw, and corn silage was used as representative of legume, grass, straw, and silage, respectively. Dry matter digestibility was higher (p < 0.001) in corn silage than in alfalfa hay. Dry matter digestibility of alfalfa hay was higher than those of tall fescue or rice straw (p < 0.001). Neutral detergent fiber digestibility of tall fescue was lower (p < 0.001) than those of alfalfa, rice straw, or corn silage. Daily enteric $CH_4$ production and the daily enteric $CH_4$ production per kilogram of $BW^{0.75}$, dry matter intake (DMI), organic matter intake (OMI), digested DMI, and digested OMI of rice straw did not differ from those of tall fescue but were higher (p < 0.001) than those of alfalfa or corn silage. Roughage type had no effect on enteric $CO_2$ emission in goats. Straw appeared to generate more enteric $CH_4$ production than legume or silage, but similar to grass.