• Title/Summary/Keyword: Lateral Flow

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Study on Application of Criteria of Lateral Flow in Soft Soils (측방유동 판정기준의 적용성 연구)

  • Chae, Young-Soo;Moon, Han-Joong;Kim, Dae-Kyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.2
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    • pp.219-223
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    • 2006
  • In this paper, the criteria of the lateral displacement in soft soil due to embankment were evaluated. The lateral displacement was measured at a pier structure in a new harbor construction site, and the measurement was compared with the criteria. The results by the measurement such as the critical pressure causing lateral movement in terms of undrained strength, the lateral movement index by Japan Highway Co., the lateral displacement index by Japan Construction Ministry, the modified index by Korean Highway Co. were estimated to be lower than the respective index values.

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Determination of Flow Direction from Flow Indicators and Lateral Grading in the Naeyeonsan Tuff, Northern Pohang, Korea (포항 북부 내연산응회암의 흐름지시자와 측방점이로부터 유향 결정)

  • Cho, Nam-Sik;Hwang, Sang Koo
    • The Journal of the Petrological Society of Korea
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    • v.26 no.2
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    • pp.153-163
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    • 2017
  • The Naeyeonsan Tuff is a stratigraphic unit which is distinguished as a cooling unit in the volcanic rocks of the northern Pohang. The Naeyeonsan Tuff, which is composed of crystals of plagioclase, quartz and hornblende, glasses of pumice and shard, and lithics of dacite, rhyolite, sandstone and shale, belongs to a lapilli tuff field according to the granulometric classification and to a vitric tuff field according to the constituent classification. The tuffs mostly develop welding foliations by dense welding and flatterning pumices and shards, and show several flow indicators by pyroclastic flowing. We can know a movement pattern from flow lineations and imbrications by pumices and lithics, and lateral gradings in isopleth map by average largest lithics and pumices in the Naeyeonsan Tuff, which indicate that the Naeyeonsan Tuff had a possible source area from the southeastern part.

A Study on Cause of Defects in NIL Molding Process using FEM (유한요소 해석을 이용한 나노임프린트 가압 공정에서 발생하는 결함 원인에 대한 연구)

  • Song, N.H.;Son, J.W.;Kim, D.E.;Oh, S.I.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2007.10a
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    • pp.364-367
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    • 2007
  • In nano-imprint lithography (NIL) process, which has shown to be a good method to fabricate polymeric patterns, several kinds of pattern defects due to thermal effects during polymer flow and mold release operation have been reported. A typical defect in NIL process with high aspect ratio and low resist thickness pattern is a resist fracture during the mold release operation. It seems due to interfacial adhesion between polymer and mold. However, in the present investigation, FEM simulation of NIL molding process was carried out to predict the defects of the polymer pattern and to optimize the process by FEA. The embossing operation in NIL process was investigated in detail by FEM. From the analytical results, it was found that the lateral flow of polymer resin and the applied pressure in the embossing operation induce the weld line and the drastic lateral strain at the edge of pattern. It was also shown that the low polymer-thickness result in the delamination of polymer from the substrate. It seems that the above phenomena cause the defects of the final polymer pattern. To reduce the defect, it is important to check the initial resin thickness.

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A Novel Nucleic Lateral Flow Assay for Screening phaR-Containing Bacillus spp.

  • Wint, Nay Yee;Han, Khine Kyi;Yamprayoonswat, Wariya;Ruangsuj, Pattarawan;Mangmool, Supachoke;Promptmas, Chamras;Yasawong, Montri
    • Journal of Microbiology and Biotechnology
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    • v.31 no.1
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    • pp.123-129
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    • 2021
  • Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that contain a class IV PHA synthase, such as some Bacillus spp. The phaR gene was developed to screen phaR-containing Bacillus spp. The phaR screening method involved two steps: phaR gene amplification by PCR and phaR amplicon detection using a DNA lateral flow assay. The screening method has a high specificity for phaR-containing Bacillus spp. The lowest amount of genomic DNA of B. thuringiensis ATCC 10792 that the phaR screening method could detect was 10 pg. This novel screening method improves the specificity and sensitivity of phaR gene screening and reduces the time and cost of the screening process, which could enhance the opportunity to discover good candidate PHA producers. Nevertheless, the screening method can certainly be used as a tool to screen phaR-containing Bacillus spp. from environmental samples.

Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips

  • Kim, Na-Kyeong;Lee, Hyo-Jeong;Kim, Sang-Min;Jeong, Rae-Dong
    • The Plant Pathology Journal
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    • v.38 no.2
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    • pp.159-166
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    • 2022
  • Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

RT-RPA Assay Combined with a Lateral Flow Strip to Detect Soybean Mosaic Virus

  • Bong Geun Oh;Ju-Yeon Yoon;Ho-Jong Ju
    • The Plant Pathology Journal
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    • v.40 no.4
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    • pp.337-345
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    • 2024
  • Soybean (Glycine max L.) is one of the most widely planted and used legumes in the world, being used for food, animal feed products, and industrial production. The soybean mosaic virus (SMV) is the most prevalent virus infecting soybean plants. This study developed a diagnostic method for the rapid and sensitive detection of SMV using a reverse transcription-recombinase polymerase amplification (RT-RPA) technique combined with a lateral flow strip (LFS). The RT-RPA and RT-RPA-LFS conditions to detect the SMV were optimized using the selected primer set that amplified part of the VPg protein gene. The optimized reaction temperature for the RT-RPA primer and RT-RPA-LFS primer used in this study was 38℃ for both, and the minimum reaction time was 10 min and 5 min, respectively. The RT-RPA-LFS was as sensitive as RT-PCR to detect SMV with 10 pg/µl of total RNA. The reliability of the developed RT-RPA-LFS assay was evaluated using leaves collected from soybean fields. The RT-RPA-LFS diagnostic method developed in this study will be useful as a diagnostic method that can quickly and precisely detect SMV in the epidemiological investigation of SMV, in the selection process of SMV-resistant varieties, on local farms with limited resources.

Lateral Earth Pressures on Buried Pipes due to Lateral Flow of Soft Grounds (연약지반의 측방유동으로 인하여 매설관에 작용하는 측방토압)

  • Hong, Byungsik;Kim, Jaehong
    • Journal of the Korean GEO-environmental Society
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    • v.11 no.9
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    • pp.27-38
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    • 2010
  • A series of model test as well as numerical analysis by FEM was performed to investigate lateral earth pressure acting on a buried pipe in soft ground undergoing horizontal soil movement. A model test apparatus was manufactured so as to simulate horizontal soil movement in model soft ground, in which a model rigid buried pipe was installed. The velocity of soil deformation could be controlled as wanted during testing. The model test was performed on buried pipes with various diameters and shapes to investigate major factors affected the lateral earth pressure. The result of model tests showed that the larger lateral earth pressure acted on the buried pipes under the faster velocity of soil movement. The result of numerical analysis, which was performed under immediate loading condition, showed a similar behavior with the result of model tests under 0.3mm/min to 1.0mm/min velocity of soil deformation. Most of model tests showed the soil deformation-lateral load behavior, in which the first yielding load developed at small soil deformation and elastic behavior was observed by the yielding load. Then, lateral load was kept constant by the second yielding load, in which plastic behavior was observed between the first yielding load and the second yielding one. Beyond the second yielding load, the compression behavior zone was observed. When the velocity was too fast, however, the lateral load was increased with soil deformation beyond the first yielding load without showing the second yielding load. The buried pipes with the larger diameter was subjected to the larger lateral load and the larger increasing rate of lateral load. At small soil deformation, the influence of diameter and shape of buried pipes on lateral load was small. However, when soil deformation was increased considerably, the influence became more and more.

A Study on Flow Characteristics with the Installed Location Change of Mechanical Deflector (기계적 편향판 설치위치의 변화에 따른 유동특성에 대한 연구)

  • Kim, Kyoung-Ryun;Park, Jong-Ho
    • The KSFM Journal of Fluid Machinery
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    • v.18 no.5
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    • pp.49-53
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    • 2015
  • Thrust vector control is the method which generates the side force and roll moment by controlling exhausted gas directly in a rocket nozzle. TVC is classified by mechanical and fluid dynamic methods. Mechanical methods can change the flow direction by several objects installed in a rocket nozzle exhaust such as tapered ramp tabs and jet vane. Fluid dynamic methods control the flight direction with the injection of secondary gaseous flows into the rocket nozzle. The tapered ramp tabs of mechanical methods are used in this paper. They installed at the rear in the rocket nozzle could be freely moved along axial and radial direction on the mounting ring to provide the mass flow rate which is injected from the rocket nozzle. TVC of the tapered ramp tabs has the potential to produce both large axial thrust and high lateral force. We have conducted the experimental research and flow analysis of ramp tabs to show the performance and the structural integrity of the TVC. The experiments are carried out with the supersonic cold flow system and the schlieren graph. This paper provides to analyze the location of normal shock wave and distribution of surface pressure on the region enclosed by the tapered ramp tabs.

Effect of stall delay characteristics of symmetrical aerofoil using lateral circular ridges

  • Raatan, V.S.;Ramaswami, S.;Mano, S.;Pillai, S. Nadaraja
    • Wind and Structures
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    • v.34 no.4
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    • pp.385-394
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    • 2022
  • Global Warming has been driven majorly by the consumption of fossil fuels. Harnessing energy from wind is viable solution towards reducing carbon footprint created due to burning such fuels, However, wind turbines have their problems of flow separation and aerodynamic stall to tackle with. In an attempt to delay the stall angle and improve the aerodynamic characteristics of the NACA 0015 symmetrical aerofoil, lateral cylindrical ridges were attached to its suction surface, at chord positions ranging from 0.1c to 0.5c. The characteristics of the original and ridged aerofoils were obtained using simultaneous pressure readings taken in a wind tunnel, at a free stream Reynolds number of Re = 2.81 × 105 for a wide range of free stream angles of attack ranging from -45° to 45°. Depending on the ridge size, a delay in stall angle varying from 5° to 20° was achieved together with the maximum increase in lift in the post-stall phases. Additionally, efforts were made to identify the optimum position for each ridge.

Predicting the lateral displacement of tall buildings using an LSTM-based deep learning approach

  • Bubryur Kim;K.R. Sri Preethaa;Zengshun Chen;Yuvaraj Natarajan;Gitanjali Wadhwa;Hong Min Lee
    • Wind and Structures
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    • v.36 no.6
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    • pp.379-392
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    • 2023
  • Structural health monitoring is used to ensure the well-being of civil structures by detecting damage and estimating deterioration. Wind flow applies external loads to high-rise buildings, with the horizontal force component of the wind causing structural displacements in high-rise buildings. This study proposes a deep learning-based predictive model for measuring lateral displacement response in high-rise buildings. The proposed long short-term memory model functions as a sequence generator to generate displacements on building floors depending on the displacement statistics collected on the top floor. The model was trained with wind-induced displacement data for the top floor of a high-rise building as input. The outcomes demonstrate that the model can forecast wind-induced displacement on the remaining floors of a building. Further, displacement was predicted for each floor of the high-rise buildings at wind flow angles of 0° and 45°. The proposed model accurately predicted a high-rise building model's story drift and lateral displacement. The outcomes of this proposed work are anticipated to serve as a guide for assessing the overall lateral displacement of high-rise buildings.