• Title/Summary/Keyword: Laser-Induced Fluorescence

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Energy Transfer Fluorescence Quenching of $Pr^{3+}$ in LaOCI

  • Kim, Taesam;Ha, Younggu
    • Analytical Science and Technology
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    • v.8 no.2
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    • pp.125-129
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    • 1995
  • The energy transfer is observed in double-activator-doped LaOCl:Pr, Tb and LaOCl:Pr, Eu system. From laser excitation and fluorescence spectra, a peculiar process for energy transfer between the activators is found. The energy absorbed by $Tb^{3+}$ is directly transferred to $Pr^{3+}$ ion. A cascade relaxation of an excited $Pr^{3+}$ level to lower level, $^1D_2$ is induced by $Eu^{3+}$, $Tb^{3+}$, which has $^7F_J$ ground levels like $Eu^{3+}$ ion, does not affect the cascade relaxation. The result represents that the quantum state of ion is not absolute condition for the energy transfer and that the energy transfer is competitive between levels of activator when the activator ions are closely located.

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Fundamentals and Applications of Multi-functional NSOM Technology to Characterization of Nano Structured Materials (다기능 NSOM (mf-NSOM) 을 이용한 나노 구조 재료 분석에 관한 원리와 응용)

  • Lee Woo-Jin;Pyun Su-Il;Smyrl W. H.
    • Journal of the Korean Electrochemical Society
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    • v.7 no.2
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    • pp.108-123
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    • 2004
  • Imaging of surfaces and structures by near-field scanning optical microscopy (NSOM) has matured and is routinely used for studies ranging from biology to materials science. Of interest in this review paper is a versatility of modified or multi-functional NSOM (mf-NSOM) to enable high resolution imaging in several modes: (1) Concurrent fluorescence and Topographical Imaging (gases) (2) Microspectroscopy (gases) (3) Concurrent Scanning Electrochemical and Topographical Imaging (SECM) (liquids) (4) Concurrent Photoelectrochemical and Topographical Imaging (PEM) (liquids) The present study will summarize some of the recent advances in mf-NSOM work confirmed and supported by the results from several other imaging techniques of optical, fluorescence, electron and electrochemical microscopy. The studies are directed at providing local information on pitting precursor sites and vulnerable areas on metal and semiconductor surfaces, and at reactive sites on heterogeneous, catalytic substrates, especially on Al 2024 alloy and polycrystalline Ti. In addition, we will introduce some results related to the laser-induced nanometal (Ag) synthesis using mf-NSOM.

Simultaneous measurement of velocity and temperature fields in micro-scale flow and its application to electrokinetic flow (마이크로 스케일 유동에서의 속도장 온도장 동시 측정 기법과 동전기 유동에의 적용)

  • Lee, Beom-Joon;Jin, Song-Wan;Kim, Young-Won;Yoo, Jung-Yul
    • Proceedings of the KSME Conference
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    • 2007.05b
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    • pp.2585-2590
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    • 2007
  • In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIF) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIF are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

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Simultaneous Measurement of Velocity and Temperature Fields in Micro-Scale Flow and Its Application to Electrokinetic Flow (마이크로 스케일 유동에서의 속도장 온도장 동시 측정 기법과 동전기 유동에의 적용)

  • Lee, Beom-Joon;Jin, Song-Wan;Kim, Young-Won;Yoo, Jung-Yul
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.31 no.7 s.262
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    • pp.644-652
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    • 2007
  • In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIE) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIE are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

Membrane Perturbation Induced by Papiliocin Peptide, Derived from Papilio xuthus, in Candida albicans

  • Lee, June-Young;Hwang, Jae-Sam;Hwang, Bo-Mi;Kim, Jin-Kyoung;Kim, Seong-Ryul;Kim, Yang-Mee;Lee, Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • v.20 no.8
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    • pp.1185-1188
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    • 2010
  • Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin against Candida albicans was investigated. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposomes further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 to 3.3 nm.

Applications of Capillary Electrophoresis and Microchip Capillary Electrophoresis for Detection of Genetically Modified Organisms

  • Guo, Longhua;Qiu, Bin;Xiao, Xueyang;Chen, Guonan
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.823-832
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    • 2009
  • In recent years, special concerns have been raised about the safety assessment of foods and food ingredients derived from genetically modified organisms (GMOs). A growing number of countries establish regulations and laws for GMOs in order to allow consumers an informed choice. In this case, a lot of methods have been developed for the detection of GMOs. However, the reproducibility among methods and laboratories is still a problem. Consequently, it is still in great demand for more effective methods. In comparison with the gel electrophoresis, the capillary electrophoresis (CE) technology has some unique advantages, such as high resolution efficiency and less time consumption. Therefore, some CE-based methods have been developed for the detection of GMOs in recent years. All kinds of CE detection methods, such as ultraviolet (UV), laser induced fluorescence (LIF), and chemiluminescence (CL) detection, have been used for GMOs detection. Microchip capillary electrophoresis (MCE) methods have also been used for GMOs detection and they have shown some unique advantages.

Experimental Study on the Stability Enhancement of Nonpremixed Flames in Coflow Jets (동축류 제트에서 전기장에 의한 화염 안정성 증진에 대한 실험적 연구)

  • Won, Sang-Hee;Ryu, Seung-Kwan;Chung, Suk-Ho;Cha, Min-Suk
    • 한국연소학회:학술대회논문집
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    • 2007.05a
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    • pp.191-196
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    • 2007
  • The enhancement of flame stability in coflow jets has been investigated experimentally by observing the liftoff behaviors of nonpremixed propane and methane flames in the electric fields. The liftoff or blowoff velocities has been measured in terms of the applied AC voltages and frequency. The experimental results showed that the liftoff velocity could be extended significantly just by applying the high voltage to the central fuel nozzle both for propane and methane. As increasing the applied voltage, the liftoff velocity increases almost linearly with the applied voltage and have its maximum value at certain applied voltage. After that, the liftoff velocity showed decrease with the applied voltage. Through the experimental observation, we found that the liftoff velocity could be correlated well with the applied voltage and frequency in the linearly increasing regime. And after having maximum in the liftoff velocity, it was observed that the liftoff velocity decreases with the applied voltage irrespective of AC frequencies. To visualize the change of flame structure with electric fields, planar laser induced fluorescence technique was adopted, and the enhancement of flame stability has been explained based on the flame structural change in electric fields.

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A MODEL FOR THE PENETRATION RATE OF A BOUSSINESQ STARTING FORCED PLUME

  • LAW ADRIAN WING-KEUNG;AI JIAO JIAN;YU S.C.M
    • Proceedings of the Korea Water Resources Association Conference
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    • 2005.09b
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    • pp.951-951
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    • 2005
  • The characteristics of Boussinesq starting forced plumes were investigated in this study. Two distinct periods in the transient plume penetration were identified, namely the Period of Flow Development (PFD) and Period of Developed Flow (PDF). PFD refers to the time period whereby the penetration rate is governed by the complex vortex dynamics initiated by the exit conditions that can include vortex coalescence, vortex leapfrogging, pinching off of the head vortex from the trailing stem and the eventual reconnection. The pinch-off and reconnection leads to an overshoot of the plume front which is a common observation reported in previous studies. The penetration rate in PDF is more predictable and depends on the continuous feeding of buoyancy and momentum into the head vortex by the trailing buoyant-jet stem. Similarity solutions are developed for PDF to describe the temporal variation of the penetration rate, by incorporating the behavior of an isolated buoyant vortex ring and recent laboratory results on the trailing buoyant jet. In particular, the variations of velocity ratios between the head vortex and trailing buoyant jet are analytically computed. To verify the similarity solutions, experiments were conducted on vertical starting forced plumes using planar laser induced fluorescence (PLIF).

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An Experimental Study on Droplet Size Characteristics of Liquid Jets in Subsonic Crossflow (수직분사제트에서 액적크기특성에 대한 실험적 연구)

  • Kim, Min-Ki;Song, Jin-Kwan;Kim, Jin-Ki;Yoon, Young-Bin
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2006.11a
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    • pp.59-63
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    • 2006
  • A direct photograph measurement technique was used to determine the spatial distribution of the spray droplet diameter in subsonic crossflow and it also obtain that SMD distribution by using PLLIF technique. The injector internal flow was classified as three modes such as a normal, cavitation, and hydraulic flip. The objectives of this research are getting a droplet distribution and drop size measurement of normal flow and compare with the other flow effects. Although the study showed visually that drop size were spatially dependent of Air-stream velocity, fuel injection velocity, and normalized distance from the injector exit length.(x/d, y/d) There are also difference characteristics between cavitation, hydraulic flip and the normal flow.

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Dynamics of OH Production in the Reaction of O(1D2) with Cyclopropane

  • Jang, Sungwoo;Jin, Sung Il;Kim, Hong Lae;Kim, Hyung Min;Park, Chan Ryang
    • Bulletin of the Korean Chemical Society
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    • v.35 no.6
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    • pp.1706-1712
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    • 2014
  • The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).