• 제목/요약/키워드: Laser Induced Fluorescence

검색결과 248건 처리시간 0.039초

다기능 NSOM (mf-NSOM) 을 이용한 나노 구조 재료 분석에 관한 원리와 응용 (Fundamentals and Applications of Multi-functional NSOM Technology to Characterization of Nano Structured Materials)

  • 이우진;변수일
    • 전기화학회지
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    • 제7권2호
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    • pp.108-123
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    • 2004
  • 최근 근접장 광학주사현미경 (NSOM)을 이용한 재료의 표면 및 구조 분석은 생물학에서 재료과학에까지 광범위하게 응용되고 있다. 본 총설에서는 기존의 NSOM을 여러가지 현미경법 (광학, 형광, 전자 및 전기화학 현미경 관찰법)과 접목하여 구성한 다기능 NSOM (multi-functional NSOM, mf-NSOM)을 이용, 나노 재료의 고분해능 이미징에 대한 원리와 응용을 고찰하였다. 본 mf-NSOM 기술을 이용하여 실제로 Al합금 및 다결정 Ti 표면에서의 공식 (pitting)을 일으키는 취약 지역을 광학적으로 분석한 결과를 기술하였다. 또한, mf-NSOM과 레이저 기술을 통해 나노 Ag 입자를 형성하고 실시간 분석한 연구결과에 대해서도 소개하고자 한다.

마이크로 스케일 유동에서의 속도장 온도장 동시 측정 기법과 동전기 유동에의 적용 (Simultaneous measurement of velocity and temperature fields in micro-scale flow and its application to electrokinetic flow)

  • 이범준;진송완;김영원;유정열
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2007년도 춘계학술대회B
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    • pp.2585-2590
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    • 2007
  • In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIF) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIF are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

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마이크로 스케일 유동에서의 속도장 온도장 동시 측정 기법과 동전기 유동에의 적용 (Simultaneous Measurement of Velocity and Temperature Fields in Micro-Scale Flow and Its Application to Electrokinetic Flow)

  • 이범준;진송완;김영원;유정열
    • 대한기계학회논문집B
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    • 제31권7호
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    • pp.644-652
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    • 2007
  • In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIE) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIE are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

Membrane Perturbation Induced by Papiliocin Peptide, Derived from Papilio xuthus, in Candida albicans

  • Lee, June-Young;Hwang, Jae-Sam;Hwang, Bo-Mi;Kim, Jin-Kyoung;Kim, Seong-Ryul;Kim, Yang-Mee;Lee, Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • 제20권8호
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    • pp.1185-1188
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    • 2010
  • Previously, papiliocin was isolated from the swallowtail butterfly Papilio xuthus and its antimicrobial activity was suggested. In this study, the antifungal mechanism of papiliocin against Candida albicans was investigated. Confocal laser scanning microscopy (CLSM) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence analysis indicated that papiliocin disturbed the fungal plasma membrane. Moreover, the assessment of the release of FITC-dextran (FD) from liposomes further demonstrated that the antifungal mechanism of papiliocin could have originated from the pore-forming action and that the radius of the pores was presumed to be anywhere from 2.3 to 3.3 nm.

Applications of Capillary Electrophoresis and Microchip Capillary Electrophoresis for Detection of Genetically Modified Organisms

  • Guo, Longhua;Qiu, Bin;Xiao, Xueyang;Chen, Guonan
    • Food Science and Biotechnology
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    • 제18권4호
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    • pp.823-832
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    • 2009
  • In recent years, special concerns have been raised about the safety assessment of foods and food ingredients derived from genetically modified organisms (GMOs). A growing number of countries establish regulations and laws for GMOs in order to allow consumers an informed choice. In this case, a lot of methods have been developed for the detection of GMOs. However, the reproducibility among methods and laboratories is still a problem. Consequently, it is still in great demand for more effective methods. In comparison with the gel electrophoresis, the capillary electrophoresis (CE) technology has some unique advantages, such as high resolution efficiency and less time consumption. Therefore, some CE-based methods have been developed for the detection of GMOs in recent years. All kinds of CE detection methods, such as ultraviolet (UV), laser induced fluorescence (LIF), and chemiluminescence (CL) detection, have been used for GMOs detection. Microchip capillary electrophoresis (MCE) methods have also been used for GMOs detection and they have shown some unique advantages.

동축류 제트에서 전기장에 의한 화염 안정성 증진에 대한 실험적 연구 (Experimental Study on the Stability Enhancement of Nonpremixed Flames in Coflow Jets)

  • 원상희;류승관;정석호;차민석
    • 한국연소학회:학술대회논문집
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    • 한국연소학회 2007년도 제34회 KOSCO SYMPOSIUM 논문집
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    • pp.191-196
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    • 2007
  • The enhancement of flame stability in coflow jets has been investigated experimentally by observing the liftoff behaviors of nonpremixed propane and methane flames in the electric fields. The liftoff or blowoff velocities has been measured in terms of the applied AC voltages and frequency. The experimental results showed that the liftoff velocity could be extended significantly just by applying the high voltage to the central fuel nozzle both for propane and methane. As increasing the applied voltage, the liftoff velocity increases almost linearly with the applied voltage and have its maximum value at certain applied voltage. After that, the liftoff velocity showed decrease with the applied voltage. Through the experimental observation, we found that the liftoff velocity could be correlated well with the applied voltage and frequency in the linearly increasing regime. And after having maximum in the liftoff velocity, it was observed that the liftoff velocity decreases with the applied voltage irrespective of AC frequencies. To visualize the change of flame structure with electric fields, planar laser induced fluorescence technique was adopted, and the enhancement of flame stability has been explained based on the flame structural change in electric fields.

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A MODEL FOR THE PENETRATION RATE OF A BOUSSINESQ STARTING FORCED PLUME

  • LAW ADRIAN WING-KEUNG;AI JIAO JIAN;YU S.C.M
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2005년도 학술발표회(2)
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    • pp.951-951
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    • 2005
  • The characteristics of Boussinesq starting forced plumes were investigated in this study. Two distinct periods in the transient plume penetration were identified, namely the Period of Flow Development (PFD) and Period of Developed Flow (PDF). PFD refers to the time period whereby the penetration rate is governed by the complex vortex dynamics initiated by the exit conditions that can include vortex coalescence, vortex leapfrogging, pinching off of the head vortex from the trailing stem and the eventual reconnection. The pinch-off and reconnection leads to an overshoot of the plume front which is a common observation reported in previous studies. The penetration rate in PDF is more predictable and depends on the continuous feeding of buoyancy and momentum into the head vortex by the trailing buoyant-jet stem. Similarity solutions are developed for PDF to describe the temporal variation of the penetration rate, by incorporating the behavior of an isolated buoyant vortex ring and recent laboratory results on the trailing buoyant jet. In particular, the variations of velocity ratios between the head vortex and trailing buoyant jet are analytically computed. To verify the similarity solutions, experiments were conducted on vertical starting forced plumes using planar laser induced fluorescence (PLIF).

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수직분사제트에서 액적크기특성에 대한 실험적 연구 (An Experimental Study on Droplet Size Characteristics of Liquid Jets in Subsonic Crossflow)

  • 김민기;송진관;김진기;윤영빈
    • 한국추진공학회:학술대회논문집
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    • 한국추진공학회 2006년도 제27회 추계학술대회논문집
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    • pp.59-63
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    • 2006
  • 횡단류 아음속유동장에서 연료의 수직 분사시 나타나는 액적영역의 액적들을 직접사진촬영으로 측정하고 PLLIF 실험을 통하여 얻은 강도 값으로 SMD분포를 측정하였다. 본 연구의 목적은 정상유동에서의 액적들의 크기 및 분포를 관찰하고, 캐비테이션 및 수력튀김 현상에 대한 액적들의 차이를 확인하는 것이다. 실험을 통하여 정상유동의 액적들은 분사차압, 공기의 유속, 침투거리, 인젝터 지름에 대한 하류방향 거리비(x/d)에 의하여 결정되며, 캐비테이션에 의한 난류강도, 유효지름에 따라 차이가 있음을 확인하였다.

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Dynamics of OH Production in the Reaction of O(1D2) with Cyclopropane

  • Jang, Sungwoo;Jin, Sung Il;Kim, Hong Lae;Kim, Hyung Min;Park, Chan Ryang
    • Bulletin of the Korean Chemical Society
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    • 제35권6호
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    • pp.1706-1712
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    • 2014
  • The OH($X^2{\Pi}$, ${\upsilon}^{\prime\prime}=0,1$) internal state distribution following the reaction of electronically excited oxygen atom ($O(^1D_2)$) with cyclo-$C_3H_6$ has been measured using laser-induced fluorescence, and compared with that following the reaction of $O(^1D_2)$ with $C_3H_8$. The overall characteristics of the OH internal energy distributions for both reactions were qualitatively similar. The population propensity of the ${\Pi}(A^{\prime})$ ${\Lambda}$-doublet sub-level suggested that both reactions proceeded via an insertion/elimination mechanism. Bimodal rotational population distributions supported the existence of two parallel mechanisms for OH production, i.e., statistical insertion and nonstatistical insertion. However, detailed analysis revealed that, despite the higher exoergicity of the reaction, the rotational distribution of the OH following the reaction of $O(^1D_2)$ with $C_3H_8$ was significantly cooler than that with cyclo-$C_3H_6$, especially in the vibrational ground state. This observation was interpreted as the effect of the flexibility of the insertion complex and faster intramolecular vibrational relaxation (IVR).

Photodissociation of Methane at Lyman Alpha (121.6 nm)

  • Park, Jae-Hong;Lee, Jung-Woo;Sim, Ki-Jo;Han, Jin-Wook;Yi, Whi-Kun
    • Bulletin of the Korean Chemical Society
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    • 제29권1호
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    • pp.177-180
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    • 2008
  • Laser induced fluorescence studies of hydrogen atom using four wave mixing technique are reported for the photodissociation of CH4 and its isotopomers at Lya (121.6 nm). The source of dissociating and probe radiation is one and the same (delay time??20 nsec). The average translational energy of ejected hydrogen atoms (50 Kcal/mol) reveals that CH4 + hn??CH3 + H(2S) and CH4 + hn??CH2(a1A1) + H2(1Sg) are the main dissociation processes. The absolute quantum yield for CH4 and CD4 are the same, FH(CH4) = FD(CD4) = 0.31 0.05. If one divides the experimental H/D ratios from the isotopomers CH3D, CH2D2, CHD3 by the isotopic H/D ratios, a value 2 is obtained in all three cases. Overall, the heavier D atoms are more likely than the H atoms to remain attached to the carbon atom.