• The Korean Journal of Zoology
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• v.37 no.1
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• pp.76-87
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• 1994

High density lipophorin-L (HDLP-L) was purified from the hemolymph of Bombyx modi using KBr density gradient ultracentrifugation and gel permeation chromatographv (Sephadex G-2001. Lipophorin has native molecular weight of 730 Nd and consists of Apo-Lp I and Apo-Lp II with molecular weights of 250 Kd and 90 Kd, respectively. Lp contains large amounts of glutamine & glutamic acid, threonine, leucine but small amounts of cysteic acid & oxidized cystine, tyrosine, methionine. Lp also contains diacylglycerol, cholesterol, phosphatidylcholine, and phosphatidylethanolamine. Anti-lipophorin showed positive reaction with fat body and ovarial extracts and also revealed immunological identity with lipophorin of Fall webworm, Hyphantria cunea. Lipophorin maintains constant level during larval and pupal stapes but greatlv increases during adult stage in both male and female. Apo-Lp III was purified from adult hemolymph. Hemolymph was subjected to KBr ultracentrifusation and Lp-free fraction was submitted to cation exchange chromatosraphy after ammonium sulfate precipitation. Apo-Lp III has molecular weight of 17 Kd, and similar amino acid composition ar those of other species Lp but contains high amounts of tryptophan which other are tacking in.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.2
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• pp.39-44
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• 2011

To evaluate the growth rate of larval and pupal parameters of silkworm Bombyx mori fed with Silver Nanoparticles (AgNps) treated $MR_2$ mulberry leaves, the following works have been considered. The AgNp was synthesized by chemical reduction method, it was diluted by different concentrations such as 25%, 50%, 75% and 100% (without dilution). Fresh mulberry leaves (Morus alba L.) were sprayed by each concentration and were fed to silkworms, from $3^{rd}$, $4^{th}$ and $5^{th}$ instar, five feedings/day. Group $T_1$ larvae received $MR_2$ mulberry leaves sprayed with distilled water and served as control, group $T_2$, $T_3$, $T_4$ and $T_5$ larvae received 25%, 50%, 75% and 100% AgNps sprayed mulberry leaves, respectively. Silkworm larvae fed on M. alba ($MR_2$) leaves sprayed with 25% concentration of AgNps (group $T_2$) was significantly increased the larvae and cocoon length, width and weight as compared to those fed on control (group $T_1$) $MR_2$ mulberry leaves and other groups ($T_3$, $T_4$ and $T_5$). Hence, 25% AgNps dose was fixed as an effective dose. It has been observed from the present study that 25% AgNps treated (group $T_2$) leaves fed by silkworms have enhanced the larval and pupal growth and quantity of silk production than control.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.471-478
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• 2021

Effective microorganisms (EM), which are sources of fermentation byproducts in herbal medicine, were compared with oyster mushrooms(OM) to identify alternative larval feeds for white-spotted flower chafer (Protaetia brevitarsis seulensis). The nutritional composition of each fermented feed was analyzed. The content of crude protein, crude fat, and fiber was higher in the OM fermented feed, except for crude ash. No difference in nutritional content of larvae based on fermented feed was observed. A comparative weekly analysis of the live weights of larvae was based on the fermented feed used. The average weight was significantly higher in the feeds using EM and OM from third week of observation. The larval survival rate in the presence of fermented feed was 96.7% compared with 9.8% with non-fermented feed. The results suggest that fermented food source is essential for the growth of white-spotted flower chafer, and OM was a stable alternative to EM as a fermentation source for the survival of white-spotted flower chafer.

• Journal of Aquaculture
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• v.22 no.1
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• pp.105-111
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• 2009

In this study, we carried out an experiment for estimation the larval digestibility in aspects which digestive enzymatic activities and nutrition of the rotifers, Brachionus rotundiformis. Thus we enhanced the digestive enzymatic activity through the addition of starch for the increase of digestibility of rotifer (starch-rotifer), and compared with the feed efficiency through rearing of the olive flounder, Paralichthys olivaceus used rotifer lipid-enriched with Algamac $2000^{(R)}$ (CE-rotifer). The digestive enzyme activities (except for TG-lipase), total protein contents, total essential amino acid, essential amino acids (methionin and phenylalanine) of starch-rotifer (the rotifer used a starch as additive, and enriched not) was assayed significantly higher than CE-rotifer (P<0.05). And total lipid, lipid classes (except for sterol) and fatty acids as DHA and EPA showed higher in CE-rotifer than starch-rotifer (P<0.05). But, sterol contents and ST/TG ratio were shown significantly higher in starch-rotifer (P<0.05). The flounder larvae supplied the two rotifers showed standard length and body weight that not significantly differed with ranges $3.72{\sim}3.79\;mm$ and $32.9{\sim}37.8\;mg$/larva on 6 days after hatching (DAH), respectively (P>0.05). However, these of 12 DAH showed the values of significantly higher to $5.94{\pm}0.249\;mm$, $144.0{\pm}23.86\;mg$/larva and $26.2{\pm}12.13%$ in standard length, body weight and survival in CE-flounder than that of starch-flounder (P<0.05). The hydrolytic enzymatic activities of flounder larvae severally supplied the two rotifers showed the significantly higher activities in acidic -amylase, neutral -amylase, TG-lipase, lysozyme and acidic phosphatase in starch-flounder on 5 DAH (P<0.05). But neutral $\alpha$-amylase, three proteases and two phosphatases of CE-flounder on 11 DAH showed the significantly higher activities than that of starch-flounder (P<0.05). Therefore, for the flounder, Paralichthys olivaceus larvae just depleted yolk was more beneficial to supply the feed, rotifer, enhanced the digestibility than to supply the feed lipid-enriched for aspect of larval digestibility up to 6 DAH, thereafter nutrition of absorption due to the development of digestive organs suggested that enrichment effect appeared with larval somatic growth. Consequently, investigation more detailed about the larval digestive physiological and nutritional requirement variations after 6 DAH will be necessary, thereafter.

• International Journal of Industrial Entomology and Biomaterials
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• v.35 no.1
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• pp.22-29
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• 2017

Increased alcohol consumption is a burden on the world because it is associated with various health problems. However, the effects of silkworms on alcohol metabolism have not been studied yet. The hard-to-eat mature silkworms have become easier to ingest recently due to the development of technology, steam-lyophilising mature silkworm larvae. In this study, we investigated and compared the effects of SMSPs from three silkworm varieties, Baekokjam, Golden-silk and Yeonnokjam weaving white, golden, and light green cocoons on alcohol metabolism in vivo. Sprague-Dawley rats pretreated with three SMSPs (0.1 g/kg or 1 g/kg body weight) or normal diet (AIN-76A) for 2 weeks were subjected to intragastric administration of absolute ethanol (3 g/kg body weight, 3 h). Three SMSPs did not affect the final body weight and liver weight. All 3 SMSPs were effective to reduce the enzymes in alcohol metabolism, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), and liver damage and enzymes involved in liver damage, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Among SMSP from 3 varieties of silkworm, preadministration of 1 g/kg Baekokjam SMSP showed the most effective suppressive effect on the activities of ADH, ALDH, AST and ALT. The Baekokjam SMSP contained higher amounts of beneficial amino acids than Golden-silk or Yeonnokjam SMSP. These results suggest that Baekokjam SMSP might be used as a new and promising candidate for improving alcohol metabolism and liver injury through promoting rapid alcohol metabolism.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.407-414
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• 1997

The resistance of a non-transgenic poplar clone, 'Ogy' and three transgenic poplar lines to the cottonwood leaf beetle, Chrysomela scripta F., was evaluated by in vitro feeding. The lines were transformed with neomycin phosphotransferase II(NPT II) as a selectable marker, proteinase inhibitor II(pin2) as a resistance gene, and CaMV 35S as a promoter. An efficient method of sterilizing the beetle eggs and introducing them into plant tissue cultures was developed. The resistance of the transgenic lines was investigated in terms of effects tin leaf area consumed, insect weight, insect developmental stages, and plantlet root dry weight after feeding. Also, leaf area consumed was examined by leaf age as measured through leaf plastochron index(LPI). The leaf area consumed and insect weight were highly significant between transformants and control, and insect development in vitro was significant among the transgenic lines. Larval infestation was the most severe around LPI 4 to 5 which were young leaves. The system provided a quick, highly controlled method to screen developing transgenic plantlets directly.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.101-103
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• 2001

The wild silkmoth, Samia cynthia pryeri, was firstly collected at Miryang, Korea. The development and quantitative characters of S. cynthia preyri in the laboratory condition were analysed. The larval body color of dark yellow green or light yellow green of the S. cynthia pryeri was changed into white color during the molting period, and the yellowish bristles in younger larvae were changed into fade green color in advanced stage larvae. Aralia elata and Zanthoxylum piperitum were newly identified as host plants to S. cynthia pryeri. Cocoon weight and cocoon shell percentage were average 2.14 g and 12.3 %, respectively. In addition, the morphology of the silkgland was different from that of the domestic silkworm,Bombyx mori, but was identical with that of the wild silkmoth, Antheraea yamamai.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.93-98
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• 2005

Studies on extended egg preservation schedule from 120 days to 180 days was taken up with 20 germplasm accessions of mutant silkworm genetic stocks of Bombyx mori L. Statistical analyses of the data collected over three trials revealed no significant changes both in the qualitative and quantitative traits of the genetic stocks between treatment (6 months egg preservation) and control (4 months egg preservation), except for fifth instar larval duration in TMS-61, TMS-62, TMS64, TMS-31 and TMS-34 shell weight in TMS-62, TMS-64 and TMS-66. Thus, the results indicate that extended schedule of 6 months egg preservation can safely be adopted, which will reduce the cost of conservation and minimize the genetic erosion owing to reduced crop cycle.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.305-312
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• 1995

The Injection of viable Escherichia coli K-12 with fifth instar larvae of cabbage butterfly, Artogeia rapae, induced at least five groups of proteins with the antibacterial activity against certain Gram-negative and/or Gram-positive bacteria in the haemolymph. These antibacterial proteins were separated and one was purified by different types of chromatography. The purified protein was heat-stable and basic peptide, and its molecular weight was approximately 4 kDa. We propose the name hinnavins for this antibacterial peptide.

• Journal of Sericultural and Entomological Science
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• v.35 no.1
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• pp.28-35
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• 1993

Treatment of juvenile hormone analogue(JHA) at the 5th instar larvae prolonged the duration of adult development one to one and half days as well as the elongation of feeding time with the increasing of larval body weight. Morphological observation and protein analysis in hemolymphs, integuements, alimentary canals, fat bodies and ovaries also revealed that the development of these tissues and organs for adultation are affected by the JHA treatment.