• Title/Summary/Keyword: Larval mortality

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Screening of Botanicals Against Root Knot Disease Complex in Mulberry (Morus indica L.)

  • Naik V. Nishitha;Sharma D.D.;Govindaiah Govindaiah;Chowdary N.B.;Mala V.R.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.14 no.1
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    • pp.57-61
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    • 2007
  • For developing an integrated eco-friendly package against root knot disease complex of mulberry caused by the association of Meloidogyne incognita with Fusarium solani and F. oxysporum causing serious loss in terms of leaf yield and quality during cultivation, twenty botanical extracts at 5, 10 & 20% concentrations were screened under in vitro conditions. Among the extracts, Allium sativum followed by Lasownia inermis were found to be effective at 20% concentration against both the virulent fungi and nematode. Both the extracts reduced the mycelial growth of virulent fungi to an extent of 76-100%, inhibited the hatching of nematode eggs by 80-90% and 76-85% larval mortality over the control. The other extracts were found either moderately or poorly effective in reducing the growth of fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective botanical extracts, which rated as strong inhibitors against both nematode and virulent fungi, can be utilized in developing an integrated ecofriendly technology for better management of root knot disease complex in mulberry.

Distribution and Mortality of Eggs and Larvae of Anchovy, Engraulis japonica, in the Southern Coastal Waters of Korea (한국(韓國) 남해(南海) 연안(沿岸)의 멸치 난(卵) 자어(仔魚) 분포(分布)와 사망율(死亡率)의 변동(變動))

  • Kim, Jin-Yeong;Kim, Joo-Il
    • Korean Journal of Ichthyology
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    • v.5 no.2
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    • pp.217-225
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    • 1993
  • A study on the distribution and mortality of eggs and larvae of anchovy. Engraulis japonica, was carried out on the basis of data collected from ichthyoplankton survey in the southern coastal waters of Korea from June to August in 1990. Egg and larval distribution areas were shifted from the adjacent waters of Namhae Is, to the eastern waters of Goeje Is, while eggs and larvae were developed in June. However, high densities of eggs and larvae of anchovy and were found near Goeje Is. in July. Anchovy eggs and larvae were correlated significantly with zooplankton in July, and were not in June and August. It is likely that the low instantaneous mortality coefficients in eggs and high values in the larvae during June and high values in eggs and low values in the larvae during July were affected by the distribution pattern of zooplankton.

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Efficacy of Wood Ash as an Antiviral Agent against Cytoplasmic Polyhedrosis Virus of Tasar Silkworm, Antheraea mylitta D.

  • Singh, G.P.;Roy, D.K.;Sahay, Alok;Suryanarayana, N.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.13 no.2
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    • pp.97-101
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    • 2006
  • The efficacy of wood ash from Terminalia arjuna (arjun) and T. tomentosa (asan) has been tested against virosis of tasar silkworm, Antheraea. mylitta D. The Polyhedral Occlusion Bodies (POBs) of Cytoplasmic Polyhedrosis Virus of A. mylitta (AmCPV) were exposed to the aqueous solution (0.5 to 4%) of wood ash for 5 to 30 minutes. The treated suspension of POBs was orally inoculated once to tasar silkworm larvae after 24 hours of $1^{st}$ moult, and larvae reared in indoor on arjun leaves till spinning. The application of aqueous solution of wood ash has established its potential as antiviral agent against cytoplasmic polyhedrosis virus. Two percent aqueous solution of wood ash from arjun and asan dissolved the Polyhedral Occlusion Bodies (POBs) of cytoplasmic polyhedrosis virus of tasar silkworm and inactivated the virions within a short period of 20 to 30 minutes. In vivo efficacy of aqueous solution of wood ash resulted in reduction of larval mortality due to virosis. The mortality was reduced to $2.56{\pm}0.21\;and\;3.03{\pm}0.32%$ when treatment of 2.0% solution of wood ash of arjun and asan respectively were applied for 20 minutes, compared to inoculated control $(92.18{\pm}7.52%)$. No mortality was recorded when treatment of 2.5% solution of wood ash of arjun and asan were applied for 10 minutes or more.

Effects of the Insect Growth Regulator Dimilin on Larval Development of Hemigrapsus sanguineus (Crustacea, Brachyura) Reared in the Laboratory (무늬발게 幼生에 對한 Dimilin 의 致死 影響)

  • Kim, Chang Hyun
    • The Korean Journal of Ecology
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    • v.13 no.1
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    • pp.19-24
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    • 1990
  • The effects of insect growth regulator Dimilin which interfere with the synthesis of chitin in the cuticle of insect larvae were investigated at various concentrations using the crab larvae of Hemigrapsus sanguineus. The larvae were cultured at control, 0.5, 1, 5 and 10 $\mu g$ Dimilin solutions and three replicate experiments were carried out to give correct analysis. Significant differences in percent mortality have occurred between control and 10$\mu g$ when the larvae were exposed to Dimilin whereas no differences were found between 5 and 10$\mu g$ Dimilin concentrations. If lethal concentration is defined as concentration at which less than 10 percent of crab larvae reach to the last zoeal stage from hatching it can be concluded that insect growth regulator Dimilin is lethal to the larvae of Hemigrapsus sanguineus at 5 and 10$\mu g$ Dimilin.

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Resistance and control of cypermethrin and chlorpyrifos as acaricide for control of hard tick Haemaphysalis longicornis (acari: ixodidae)

  • You, Myung-Jo
    • Korean Journal of Veterinary Research
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    • v.54 no.2
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    • pp.117-120
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    • 2014
  • Chemotherapeutic treatment is still the foundation of tick control programs. This study investigated the acaricidal efficacy of cypermethrin alone and in combination with chlorpyrifos against Haemaphysalis (H.) longicornis. Unfed larval ticks were exposed to 0.1, 1.0, and 10 mg/mL cypermethrin for 60 min, after which the acaricidal efficacy was examined based on tick mortality. All compounds showed similar suppression curves, with the best control being achieved by cypermethrin and chlorpyrifos (1 : 1 ratio) at 10 mg/mL. Effective cypermethrin concentrations for tick control were two to seven times higher than the recommended doses, indicating resistance by H. longicornis.

Settlement and recruitment of Mactra veneriformis R. around the inshore of Kunsan, Korea (군산연안 동죽, Mactra veneriformis의 침강과 가입)

  • RYOU Dong-Ki;CHUNG Sang-Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.5
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    • pp.667-676
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    • 1995
  • Settlement, recruitment, growth and production of Mactra venerifomis were investigated at Kunsan tidal flat located in the west coast of Korea from lune 1993 to May 1994. High density of planktonic larvae of M. veneriformis was observed between the mid and early July in 1993. The size of the newly settled larvae ranged from 250 to 350um in shell length with mean density of 601$inds.\cdot m^{-3}$. Recruitment of M. veneriformis larvae was occurred mainly in the mid June to mid July 1993. The larvae have no substratum preference during the larval settlement phase. Mortality of larval was affected by environment (substratum, exposure time, density of adult clams). After settlement they grew until November, and stopped growing in winter from December. The estimated turnover rate of M. veneriformis larvae was estimated as high as 4.91 and 4.94. Annual production of the 0-age group was estimated to be 2,900g $m^{-2}\;\cdot\;yr^{-l}$, which was relatively high compared with those of other age groups.

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Evaluation of Host Resistance of 18 Warm-Season and 20 Cool-Season Turfgrass Species and Cultivars to Spodoptera depravata(Butler) (잔디밤나방에 대한 난지형 잔디와 한지형 잔디의 내충성 평가)

  • 박봉주
    • Journal of the Korean Institute of Landscape Architecture
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    • v.31 no.4
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    • pp.74-81
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    • 2003
  • The need for insect and mite resistant turfgrass cultivars arose because of problems associated with pesticide use. Representative cultivars and genotypes of 18 warm-season turfgrass [Zoysia japonica Steud., Z. japonica${\times}$Z. metrella hybrids, Z. japonica${\times}$Z. tenuifotia hybrids, Z. matrella (L.) Merr., Cynodon dactylon (L.) Pers., C. dactylon${\times}$C. transvallensis hybrids, Paspalum notatum Flugge., P. vaginatum Swartz., Stenotaphrum secundatum (Walt.) Kuntze, Eremochloa ophiuroides (Munro.) and Buchloe dactyloides (Nutt.) Engelm.] and 20 cool-season turfgrasses [Poa pratensis L., Festuca arundinacea Schreb., F. rubra L., F. rubra var. commutata Gaud., F. ovina var. duriuscula L. Koch. Agrostis tenuis Sibth., A. palustris Huds., and Latium perenne L.] were evaluated for host resistance to feeding by the Spodoptera depravata (Butler) in the laboratory. Two experiments were set up in the laboratory using 8.5cm diameter${\times}$4.0cm deep plastic petri dishes as larvae feeding chambers. In experiment 1, one neonate larvae were place on the grass in each dish and the dishes were arranged with 5 replicates each within an environmental chamber maintained at $25^{\circ}C$ and 15h light: 9h dark Larval survival and larval weights at 7d and 14d, pupal weights, and days to pupation were compared among turfgrasses. In Experiment 2, 4cm sections of all grasses were oriented equidistant from each other in a pattern resembling the spokes of a wheel. Five one neonate larvae were introduced to the center of each dish. Dishes were immediately placed in an environmental chamber held at $25^{\circ}C$, 15h light: 9h dark Larvae were allowed to feed for 24h. Damage was rated from 0(no damage) to 9(completely consumed) were made for eachturfgrass. Resistance as antibiosis (high mortality, slowed growth, and least preference) was identified in Z. japonica${\times}$Z. tenuifolia hybirids ‘Emerald’, Z. japonica${\times}$Z. metrella hybirds ‘Miyako’ and Eremochloa ophiuroides (Munro.). Cool-season turfgrasses tested were susceptible to feeding by Spodoptera depravata (Butler).

Enriched Rotifer Feeding Efficiency in the Walleye Pollock Theragra chalcogramma Depends on Larval Fatty Acid Composition (명태(Theragra chalcogramma) 자어의 지방산 조성에 따른 영양강화 로티퍼의 먹이효율)

  • Park, Jin-Chul;Hong, Woo-Seok;Seo, Joo-Young;Nam, Won Shik;Kwon, O-Nam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.5
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    • pp.549-555
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    • 2018
  • The objectives of this study were to confirm the nutritional requirements and improve the survival of the walleye pollock Theragra chalcogramma, a cold seawater fish, by enrichment. We analyzed the fatty acids and amino acids of fertilized pollock eggs before hatching, just-hatched larvae, larvae that had absorbed only the yolk sac, and larvae starved for 2 days after yolk absorption. For the survival improvement experiment, we administered docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and DHA-EPA enrichment. Fatty acid decreased DHA and EPA content. On the $30^{th}$ day, body growth was significantly greater in pollock given the EPA and DHA-EPA treatments (P<0.05). Larval survival at 20 and 30 days after hatching (DAH) was greatest under the DHA-EPA treatment (P<0.05). Survival was significantly lower under the EPA treatment at 10 DAH, but then increased to approach that seen under the EPA-DHA treatment (P<0.05). Therefore, we determined that reduced survival in hatchlings of high-mortality pollack could be improved by controlling EPA and DHA content during enrichment. We conclude that cold seawater fish must be given feed that meets their nutritional needs, which can be accomplished using newly manufactured enrichment products for the larvae of cold seawater fish, such as pollock.

Foraging Behavior of Helicoverpa armigera $H{\ddot{u}}bner$ (Lepidoptera: Noctuidae) First Instar Larvae on Selected Cotton Varieties

  • Amin, Md. Ruhul;Azad, H.M. Saifullah;Hossain, Md. Shamim;Suh, Sang Jae;Kwon, Yong Jung
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.4
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    • pp.185-188
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    • 2014
  • The movement, survival, and weight gain of Helicoverpa armigera $H{\ddot{u}}bner$ (Lepidoptera: Noctuidae) first instar larvae were studied on CB9, CB10 and SR05 cotton varieties under field conditions. The neonate H. armigera were released on the cotton varieties at the squaring stage of the plants and, after a period of 72 hours, the survival, weight gain, and final location of the larvae were observed. While the different cotton varieties had no effect on the survival and weight gain of the larvae, the release locations on the cotton varieties had a significant influence on the larval survival and weight gain. The larvae fed small squares of the cotton varieties were significantly heavier and showed a higher mortality than the larvae fed leaflets and mature leaves. For the cotton varieties in this study, the larvae released on leaflets showed a significantly higher rate of recovery compared to the larvae released on mature leaves and squares. This study also found that that the larvae on leaflets did not move up or downward unlike the larvae on mature leaves and squares. This information on the foraging behavior of larvae on cotton varieties will assist researchers to interpret field data and thereby help with the development of pest management decisions.

Microbial Control of the Tobacco Cutworm, Spodopera litura (Fab.), Using S. litura Nuclea Polyhedrosis Virus. II. Formulation of S. litura Nuclear Polyhdrosis Virus as Viral Insecticides (곤충 핵다각체병바이러스를 이용한 담배거세미나방의 생물적 방제. II. 담배거세미나방 핵다각체병바이러스의 살충제 제제화)

  • 임대준;진병래;최귀문;강석권
    • Korean journal of applied entomology
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    • v.29 no.4
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    • pp.244-251
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    • 1990
  • Three viral insecticides were differently formulated with a nuclear polyhedrosis virus isolated from Spdodoptera litura by addition of feeding attractant, anti-precipitate of polyhedra, spreading agent, and UV-protectants. Sucrose was effective for attraction of larval feeding to increase the mortality and for protection of polyhedra from inactivation by sunlight when added 1% to 5% of sucrose solution to the formulations. Contents of additives to the formulations were 0.5% in polyvinyl alcohol to prohibit the precipitation of polyhedra and 0.1% in Triton X-100 to spread and wet the formulations to the plant. Inactivation of the virus under sunlight was decreased when added 800g of white carbon to 100 L of water in the white carbon formulation and 30% of molasses to the molasses's. In the formulation of white carbon and molasses mixtures, activation of the virus was increased when mixtured 500g of the former with 10% of the latter. Three formulations were persisted their pathogenicity more than 95% of mortality at 3 days p.i. Encapsulation of the polyhedral surface was more distinctively coated with the carbon and showed more effective in the residual effects of the white carbon than others, but the molasses more attractive for larval feeding.

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