• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.6
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• pp.457-467
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• 1990

Sessile barnacle, Balanus trigonus Darwin, was collected from the intertidal rocks and the external shells of bivalve Mylius coruscus. The nauplius and cyprid larvae were cultured in a constant temperature cabinet at a temperature of $20^{\circ}C$ and a light regime of 14 h light and 10 h darkness. Larval development includes the six nauplius stages and a cyprid stage prior to settlement and metamorphosis to the young adult. Morphological characteristics including antennules, antennae and mandibles are described and illustrated. All nauplius larvae have trilobed labra typical in balanoides. The developmental time taken from newly hatched nauplius I to cyprid was $9\~13$ days and averaged 11 days.

• Ocean Science Journal
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• v.40 no.3
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• pp.177-182
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• 2005

The process of embryogenesis and larval development of the asteroid sea star Asterias amurensis $(U{\ddot{u}}tken)$ was observed, with special attention paid to morphological change and larval duration. In reproductive season, mature sea stars were collected under floating net cages, located in Tongyeong, southern Korea. The mature eggs are $138\;{\mu}m$ in average diameter, semi-translucent and orange in color, sperms in good condition appear light cream to white-gray in color. Embryos develop through the holoblastic equal cleavage stage and a wrinkled blastula stage that lasts about 9 hours after fertilization. Gastrulae bearing an expanded archenteron hatch from the fertilization envelope 22 hours after fertilization. At the end of gastrulation, rudiments of the left and right coelom are formed. By day 2, larvae possess complete alimentary canal and begin to feed. At this stage, the larva is called early bipinnaria. In 6-day-old larvae, the pre- and post- oral ciliated bands form complete circuits and the bipinnarial processes start to develop. By day 12, the lateral and anterior projection of the larval wall processes along the ciliated bands begins to thicken and curl, and the ciliated bands become more prominent. By day 32, early brachiolaria are presented with three pairs of brachiolar arms. Advanced brachiolaria with a well-developed brachiolar complex (three pairs of brachia and central adhesive disc) occur 6 weeks after fertilization. In the field, spawning of the sea star was observed in April to May, settlement form larvae and just settlements seem to occur from June to July, and early juveniles occur from August to September. Although we had not described the end of brachiolaria stage, it can be tentatively estimated that the duration of the pelagic stage of A. amurensis is 40 to 50 days.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.558-568
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• 2023

The spatio-temporal distribution of the sand crab Ovalipes punctatus larvae was investigated in the Korean waters in 2019. Sea surface temperature (SST) was the lowest in February and highest in September. Sea surface salinity (SSS) was the lowest in September and highest in March. Further, sea surface chlorophyll a (SSC) was the highest in September. Larvae were distributed in the South Sea and coastal area of Jeju Island from April to June, and the abundance was the highest in May. The spatio-temporal distribution analysis suggested that larval groups showed a tendency to be dispersed over a wider area as the larvae developed, due to the increase in their swimming ability. The correlation analysis between environmental factors and larval density suggested that larvae appeared in the SST range 11.8-20.9℃ and SSS range 31.5-35.3 psu. The Megalopal stage appeared in a wider range of SST and SSS than other larval stages, possibly due to the increased environmental tolerance before settlement. Results of redundancy analysis (RDA) and Spearman's rank correlation analysis between the larval density by developmental stages and the environmental factors suggested that SST showed a positive correlation and SSC showed a negative correlation in the later stage.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.21-28
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• 2012

The combined effects of water temperature and salinity on the settlement rate and density (spats/$cm^2$) of Crassostrea gigas pediveliger larvae were studied under the hatchery conditions. Four water temperatures (20.0, 23.0, 26.0 and $29.0^{\circ}C$) were tested at three salinities (20.0, 30.0 and 40.0). The optimum water temperature by salinity conditions were $29.0^{\circ}C$ and 20.0, 30.0 which significantly (P < 0.05) the highest mean (${\pm}S.D$) larval settlement rate of $43.1{\pm}0.1%$, $42.1{\pm}0.1%$, respectively. But the combination of water temperatures and salinities for settlement density was not effected, but each factor as water temperature ($29.0^{\circ}C$), salinity (40.0) was shown significantly high and low settlement density, respectively (P < 0.05). The statistics result of settlement density frequency was appeared: mode ($1.2-2.3spats/cm^2$), Mean ($1.9-2.4spats/cm^2$), Range ($3.0-5.8spats/cm^2$) and the lowest variance was obtained 0.4 at water temperature $20.0^{\circ}C$ by salinity 30.0, but $29.0^{\circ}C$ by 20.0 was the highest (1.1). The best condition at this results for the larvae setting under the artificial seed collection of C. gigas was $29.0^{\circ}C$ and 20.0-30.0.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.970-972
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• 2013

We examined the relative growth of Microstomus achne during early life stages of laboratory-reared larvae and juveniles. Turning points in the relative growth of preanal length and upper jaw length against total length occurred during the settlement period (11.12-19.91 mm in total length). However, turning points in the relative growth of head length and eye diameter, as compared to total length, occurred during metamorphosis (17.57-22.47 mm in total length). Our results suggest that Microstomus achne concentrates its energy on the feeding apparatus (jaw) and digestive organs (intestine) rather than sensory or neural organs (eye, head) during early larval stage growth.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.91-96
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• 2013

Although the method of seedling production of Haliotis discus hannai is well known, the optimum benthic diatom species as a live food at early larval stage are not fully developed. In this study three Pennales diatom species, Caloneis schroederi, Rhaphoneis sp., and Cocconeis californica were examined on settlement, metamorphosis, survival, and growth of Haliotis discus hannai larvae. The larvae fed Raphoneis sp. or C. californica showed high settlement rate with 80-82% within 48 hrs, which was significantly higher than those fed C. schroederi or mixed diets with three diatom species. The larvae fed the former microalgal species also showed higher metamorphosis rate with 32-34% than the latter species with 10-12% within 4 days. With regard to survival and growth of the larvae, single diet with Rhaphoneis sp. or C. californica had better dietary value than the mixed diets for the early larvae of H. discus hannai.

• Journal of Aquaculture
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• v.10 no.1
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• pp.25-32
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• 1997

Develoment precess and characteristics of eggs of the geoduck clam, Panope japonica are reporting in this study. Eggs and sperm were excised from gonad, artificially fertilized in an aquarium, reared under various temperature regimes, and record and record the larval period and the time need to reach a certain larval stage from ferilization. Unfertilized eggs of P. japonica appeared to be oval with a mean diameter of $70\mu$m and they became spherical after fertilization. The eggs of P. japonica can be classified as demersal. At a constant water temperature of $ 11^{citc}C$, it took 4 hours form fertilization to become four-cell stage, two days to become trochophore larvae, three days to become D-shape larvae, twenty-three days to become umbo stage, and thirty-six days to become fully grown veliger ready form settlement. A negative correlation was observed between the water temperature and the larval period of P. japonica. From fertilization to D-shape larvae, it took five days at 8$^{\circ}C$, while it was only two days to become D-shape larvae at $ 17^{citc}C$. Time required to D-shape larvae from fertilization was proportional to temperature, and the relationships were expressed as follows : To 8-cell stage, 1/t=0.0209 w-0.1167 (r=0.9967) To blastula stage, 1/t=0, 0055 w-0.0192 (r=0.9825) To trochophore stage, 1/t=0.0034 w-0.0155 (r=0.9907) To D-shape larvae stage, 1/t=0.0014 w-0.0023 (r=0.9843) (t, time in hours ; w, water temperature) Bioligical minimum temperature for egg development was calculated as 3.82$^{\circ}C$ in average.

• Korean journal of applied entomology
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• v.52 no.3
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• pp.243-248
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• 2013

This study was conducted to understand the settlement process of Ascotis selenaria larvae into citrus orchards with respect to oviposition site and analysis of the spatial distribution pattern of the larvae. A. selenaria eggs were not found on citrus trees in field and green house, but not on citrus trees in the field. A. selenaria larvae showed a significant clump distribution in the greenhouse. In the open citrus field, the index of dispersion was around 1.0 in most cases, with a weak clumping degree. However, the d-statistic was between -1.96 and 1.96, indicating a statistically significant random distribution. In addition, the Green's index (a clumping index) was very low in all cases, even though the clump distribution was accepted. for most samples, the probability distribution of larval frequency in the field satisfied the probability distribution functions of Poisson (random pattern) and the negative binomial (clump pattern) distribution. In addition, the temporal distribution of the larvae in the open field showed a pattern which was formed by colonizers from outside oviposition sites. Further, the difference in larval spatial distribution between field and greenhouse orchards was discussed.

• Journal of Aquaculture
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• v.12 no.1
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• pp.39-45
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• 1999

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995. Spawning induction rate by the water temperature was 6.0~17.5% and air dry was responsed 1.4~4.0%. Number of eggs spawned of Stichopus japonicus were $50~500\times10^4$ individuals, the fertilization and hatching rate were ranged 84.0~96.0%, 71.4~84.6% respectively. The fertilized egg of Stichopus japonicus appeared mean diameter of $154{\mu}m$. At a constant water temperature of $23^{circ)C$, it become 4 tell stage from fertilization after 2 hours 10 minutes, hatching larvae after 14 hours half, auricularia larvae after 3 days, doliolaria larvare after 11 days and pentactula larvae after 15 days ready for settlement. The suitable food in the larvae reared for 17 days after fertilization were shown the best growth and survival in the larvae food of Chaetoceros calcitrans. Optimum density for larvae rearing were maintained of the larval density lower than 2 individuals/ml.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.43-55
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• 1976

Fertilization and early development of turbo cornutus was studied based on the samples which were collected in Yeosu area. Particular emphasis was paid on induction of artificial spawing, fertilization rate, preembryonic development, the growth of the early larva and larval survival to various salinity. Among the various methods for induction of artificial spawning which have been tested for the present study, drying by exposure to air is the. most efficient, and percentage fertilization rate was $83.8-96.4\%$. The diameter of fertilized eggs was $0.182{\pm}0.0028mm$; and the diameter of egg membrane was $0.245{\pm}0.093mm$. Under the temperature range of $20.6-25.4^{\circ}C$ the larvae hatched out after 11:05-11:15 hours of fertilization. After 3.0-3.5 days of fertilization the planktonic larvae begand to settle, and the settlement terminated within 5 days. During the period of 150 days of early culturing the diameter growth of shell(M) and the diameter of shell aperture(A) was formulated as follows: $$1972\;M=0.33e^{0.02070D}$$ $$A=0.19e^{0.02282D}$$ $$1973\;M=0.32e^{0.02282D}$$ $$A=0.16e^{0.02596D}$$ During the same period of early culturing the relative growth of shell diameter and the diameter of shell aperture was formulated as follows : 1972 A=0.6478 S-0.1575 1973 A=0.5897 S-0.0515 After 11 days of larval hatching $0.02-0.18\%$ of planktonic larvae settled. After 150 days of settlement the survival rate of the early shells was $7.4-21.6\%$. Under the temperature range of $21.0-22.7^{\circ}C$ the optimum salinity range for the development of egg and the planktonic larvae was $30-35\%_{\circ}$.