• The Korean Journal of Malacology
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• v.21 no.1
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• pp.65-70
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• 2005

The purpose of this study is to determine the appropriate substrate for larval settlement and spat growth in the purple clam, Saxidomus purpuratus in laboratory culture. Larvae were reared with 3 different types of sediments (mud, sand, and mixed) for 46 days in settlement experiment, and settled spats were further grown in 3 types of sediments for 36 weeks in growth experiment. The density of settled spats in muddy sediments was more than 2 times higher than those in mixed or sandy sediments. But, the average size of settled spats in muddy sediments was smaller than those in mixed or sandy sediments. After 36 weeks of growth period, growth rate decreased as shell length increased. When shell length was less than 2 mm, growth rate in mixed sediments was significantly higher than that in sandy sediments. When shell length was more than 2 mm, there was no significant difference in growth rate among different substrates. Sediment type affected growth rate only when the spats were relatively small (less than 2 mm). Muddy sediments seems better for larval settlement, while mixed sediments is best for spat growth. We suggest the laboratory procedure for enhancing seedling production of S. purpuratus.

• Journal of Environmental Health Sciences
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• v.29 no.3
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• pp.1-8
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• 2003

The development of environmentally nontoxic or non-polluting antifouling additives that can be formulated in practical coating requires assay involving target organisms. Described here are the simple laboratory assays that have been developed using the barnacle, Balanus amphitrite, a common fouling organism found throughout temperate and tropical seas. One of the assays depends on synchronous year-round mass culture, the procedure for which is described, of nauplii larvae and cyprids larvae. The laboratory assays provided quantitative estimates of toxicity and settlement inhibition of the test compounds. Laurinterol (1), isolaurinterol (2), alpysinal (3), and aplysin (4) have been isolated from the Korean red alga Laurencia okamurae. Their structures were identified by spectral data in comparison with the literature data. Compounds 1-4 inhibited larval settlement of the barnacle B. amphitrite with EC$_{50}$ values of 0.18- 36 $\mu\textrm{g}$/ml. Com-pounds 2-4 showed larval toxicity against nauplii of the barnacle B. amphitrite with 5-10 $\mu\textrm{g}$/ml, while laurinterol (1) exhibited no toxicity at even 100 $\mu\textrm{g}$/ml. Therefore, laurinterol was expected as a promising natural antifoulant.t.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.343-351
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• 2014

We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), ${\gamma}$-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of $42.7{\pm}2.7%$, followed by GABA ($35.4{\pm}2.0%$), 5-HT ($29.1{\pm}2.2%$), L-DOPA ($19.2{\pm}2.1%$), epinephrine ($15.2{\pm}0.9%$), and norepinephrine ($11.0{\pm}1.2%$). The chemicals ${\gamma}$-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of $1.97{\pm}1.42$ and $2.37{\pm}1.86ind/cm^2$, reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.139-146
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• 2013

We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), ${\gamma}$-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of $42.7{\pm}2.7%$, followed by GABA ($35.4{\pm}2.0%$), 5-HT ($29.1{\pm}2.2%$), L-DOPA ($19.2{\pm}2.1%$), epinephrine ($15.2{\pm}0.9%$), and norepinephrine ($11.0{\pm}1.2%$). The chemicals ${\gamma}$-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of $1.97{\pm}1.42$ and $2.37{\pm}1.86\;ind/cm^2$, reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery.

• Ocean and Polar Research
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• v.37 no.1
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• pp.37-47
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• 2015

The effects of low salinity (fertilization success and larval survival) on the limpet Cellana grata were studied at early stages of development using the marine bioassay technique. It was shown that, under normal conditions for development from fertilization to the post veliger stage, the salinity must be not less than 20.0~35.0 psu. However, the fertilization rate and larval survival of C. grata was obviously reduced at 5.0 psu and 10.0 psu, respectively. Mass mortality was estimated to occur at <20.0 psu (48-h $EC_{50}=19.54psu$) and the survival rate of normal veliger larvae decreased with experimental time during exposure. No observed effective concentration (NOEC) and lowest observed effect concentration (LOEC) of post veliger were estimated at 30.0 psu and 25.0 psu, respectively, during 48-h exposure. The tolerance limits of the test species to salinity revealed various concentration ranges of salinity, which may reflect the physiology and ecology of the initial development stages of C. grata. These results demonstrate that reduced salinity is detrimental to the reproductive success and larval survival of C. grata, and if salinity is lowered by natural or anthropogenic sources during spawning, this would lead to decreased reproductive success and larval settlement.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.1
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• pp.132-138
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• 2020

Marphysa sanguinea is the most well-known polychaete species with a high economic value. However, this species has a high mortality in the early rearing stage of aquaculture. This study was conducted to find out the optimal substrate size and organic contents for the growth and survival rate of M. sanguinea larvae and juveniles. It was observed that the smaller grain size (<Ø 0.063 mm) and high organic contents (5-10%) induced settlement and reduced settlement time of larvae. Moreover, the growth and survival rate of larvae reached high levels at Ø 0.004-0.016 mm of grain sizes and 5-7.5% of organic contents as advantageous substrates for settlement. The survival rate of juveniles reached over 90% in less than Ø 0.016 mm substrate on 15-day experiment for different grain sizes of substrates. These results indicated that substrate compositions of less than Ø 0.016 mm of sand size and 5-7.5% of organic contents in mud will enhance the productivity of M. sanguinea at the early stage.

• The Korean Journal of Malacology
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• v.19 no.2
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• pp.95-106
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• 2003

The compositions of amino acid in 6 monocultured benthic diatoms used in aquaculture of Haliotis discus hannai were analyzed, and effects of 15 artificial synthetic amino acids on the settlement and metamorphosis of H. discus hannai larvae. Results showed that the content of L-aspartic acid was highest in all diatoms, and that of L-glutamic acid was second high. In experiment using settlement slat without benthic diatom attached, the highest settlement rate (33.3 ${\pm}$ 8.8%) was obtained with L-glutamic acid, and a higher value (16.7 ${\pm}$ 3.3%) was found with L-aspartic acid at 24 h after experimental commencement, compared to that of control (8.6 ${\pm}$ 5.1%). 80 h later the metamorphosis rates of L-glutamic acid (86.7 ${\pm}$ 10.7%) and L-aspartic acid (80.0 ${\pm}$ 3.3%) groups were higher than control group(0) and other amino acids significantly. The response rate of L-glutamic acid was the highest (62.0%), and those of L-aspartic acid (30.0%) and L-threonine (25.3%) groups were also significantly higher than control group. In the experiment using settlement slat with benthic diatom attached, the best effect of various amino acids on induction of larval settlement was obtained with L-glutamic (82.0 ${\pm}$ 6.9%) and L-aspartic acid (78.7 ${\pm}$ 5.1%) at 24 h after experimental commencement. The settlement rates of L-histidine, L-leucine, L-lysine, L-methionine, L-phenylalanine, and L-tyrosine groups were significantly lower than control group. The same differences in the metamorphosis rate at 56 h after experimental commencement and in the response rate were found. It should be noted that after 80 h the metamorphosis rates of L-histidine (74.0 ${\pm}$ 12.0%) and L-lysine (87.0 ${\pm}$ 8.8%) declined rapidly compared to those of 56 h (8.0 ${\pm}$ 12.0%; 7.7 ${\pm}$ 12.0%).

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.625-633
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• 2020

The effects of temperature and salinity on egg development and settlement of the ascidian Herdmania momus were investigated. Adult specimens were collected from the Dodu Yacht facility in Jeju Island, Korea(33°30'30.54"N, 126°27'55.46"E) in August 2018. Egg development and larval settlement were observed and recorded at 8 h intervals using a stereomicroscope, under nine temperature (10, 13, 16, 19, 22, 25, 28, 31, and 34℃), and four salinity regimens (28, 30, 32, and 34 psu). The highest hatching rate (82.8±7%) was observed at 32 psu and 25℃ and the lowest hatching rate (1.0±2%) was at 34 psu and 13℃. The developmental rate (0.222±0.0994) was highest at 28 psu and 28℃, and lowest (0.016±0.008) at 30 psu and 13℃. The highest settlement success rate (77.1±5%) was at 32psu and 25℃ and the lowest(0.1±1.0%) was at 30psu, and 13℃. The rate of settlement(0.080±0.000) was highest at 28psu and 28℃, and lowest(0.013±0.000) at 30psu and 13℃. Both hatching and settlement success rates increased as temperature increased and tended to decrease beyond an optimal temperature range. Herdmania momus preferred 30-34 psu salinity and 22-25℃ temperature. This study provides baseline information about the life history of H. momus, and important data to control the damage caused by the increase in number and distribution of this invasive ascidian.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.5
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• pp.667-676
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• 1995

Settlement, recruitment, growth and production of Mactra venerifomis were investigated at Kunsan tidal flat located in the west coast of Korea from lune 1993 to May 1994. High density of planktonic larvae of M. veneriformis was observed between the mid and early July in 1993. The size of the newly settled larvae ranged from 250 to 350um in shell length with mean density of 601$inds.\cdot m^{-3}$. Recruitment of M. veneriformis larvae was occurred mainly in the mid June to mid July 1993. The larvae have no substratum preference during the larval settlement phase. Mortality of larval was affected by environment (substratum, exposure time, density of adult clams). After settlement they grew until November, and stopped growing in winter from December. The estimated turnover rate of M. veneriformis larvae was estimated as high as 4.91 and 4.94. Annual production of the 0-age group was estimated to be 2,900g $m^{-2}\;\cdot\;yr^{-l}$, which was relatively high compared with those of other age groups.

• Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.339-345
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• 2011

Although culture techniques for the abalone Haliotis discus hannai are well known, mass culture of the benthic microalgae that are essential live food for the abalone larvae is still not practiced. This study was conducted to identify the microalgal species suitable for the growth of early larvae of H. discus hannai. The growth and attachment rates of 31 microalgal species were examined. Acrylic plates were used as the substrate. Among the 31 microalgal species, nine showing high growth and attachment rates were selected and tested for their dietary values via factors including settlement, metamorphosis, and survival rates of abalone larvae. Tetraselmis hazeni and Rhaphoneis sp. induced the highest settlement rate (65-69%) in abalone larvae. The metamorphosis rate was highest (57%) in larvae fed Rhaphoneis sp. and was also significantly higher in larvae fed Oscillatoria splendida (29%) and T. hazeni (22%) than in those fed other species. The highest survival rate of the larvae during the 15 days after metamorphosis was 67% in those fed Rhaphoneis sp., followed by T. hazeni (42%) and O. splendida (35%). In conclusion, Rhaphoneis sp. is the most suitable diatom for use as a live food for the culture of early larvae of H. discus hannai. In addition, T. hazeni and O. splendida are also potential species to be further developed and utilized in larval culture.