• Title/Summary/Keyword: Larvae and juvenile

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Early Life Cycle of Rhinogobius brunneus from Wicheon Korea (위천에 서식하는 밀어(Rhinogobius brunneus)의 초기생활사)

  • Jae-Min Park;Kyeong-Ho Han
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.1
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    • pp.44-53
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    • 2023
  • In this study, the morphological development and early life cycle of larvae and juvenile Rhinogobius brunneus found in Wicheon, Nakdong River, Korea, were investigated. The fertilized eggs were 2.20×0.68 mm (long×short) in diameter. The eggs began hatching approximately 104 h after fertilization at water temperatures of 17.3-20.5℃ (mean 18.9±1.6℃). The newly hatched yolk-sac larvae were 3.71±0.06 mm in total length (TL), with an unopened anus. Three days after hatching, the preflexion larvae were 4.37±0.16 mm in TL with yolk absorption. Twenty days after hatching, the flexion larvae were 6.50±0.22 mm in TL and the tip of the notochord was bent upward. Twenty-seven days after hatching, the postflexion larvae were 11.8±0.63 mm in TL, and the tip of the urostyle was bent at 45°. Forty days after hatching, individuals measured 18.5±0.93 mm in TL and were considered as juveniles as the number of fins became constant for each part. All the postflexion larvae had detached fins. Additionally, the melanophore was observed to be distributed on the head, centrum of the body, and dorsal fin, and there was a difference in the morphology from the water stream.

Morphological Development of Eggs, Larvae and Juvenile of the Sunrise Sculpin, Pseudoblennius cottoides (Teleostei: Cottidae) (가시망둑(Pseudoblennius cottoides)의 난발생 및 자치어 형태발달)

  • YOO Dong-Jae;HAN Kyeong-Ho;BAEK Seung-Rok;KIM Kwang-Su;HA Sung-Chan;ZANG Hu-Chun;LEE Gi-Seok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.3
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    • pp.263-269
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    • 2003
  • Morphological development of eggs, larvae and juvenile of the sunrise sculpin, Pseudoblennius cottoides were studed in the field and laboratory at the coastal Dolsan-do, Yeosu-shi from October, 2000 to April, 2001 Egg mass of Pseudoblennius cottoides in peribranchial cavity of Halocynthia hilgendorfi, were observed during late fall to winter in the study area. Fertiliged eggs were spherical in shape, demersal, adhesive, transparent and greenwish yellow color, measuring 1.84 mm (1.83-1.87 mm) in diameter. There were numerous and 17 (15-20) various-sized oil globules accounted in the yolk. Granular materials formed a mass in the yolk. Fertiliged eggs hatched at 301 hr 20 min after morula stage. Newly hatched larvae 6.31 mm (6.24-6.37 mm) in total length (TL), had a large yolk. At 3 days after hatching, the larvae, 6.77 mm (6.69-7.14 mm) in TL came out through the excurrent siphon of Halocynthia hilgendorfi. At 13 days after hatching, the larvae 12.59 mm (12.42-12.63 mm) in TL transformed to postlarval stage. At 32 days after hatching, postlarvae of TL 19.18 mm (19.01-19.46 mm) have reached the juvenile stage.

Development of Eggs, Larvae and Juvenile of the Korean Bullhead, Pseudobagrus fulvidraco (Richardson) reared in the laboratory (동자개의 형태발달과 성장특성)

  • Han, Kyung-Nam;Nam, Ki-Bong;Jeong, Choong-Hoon
    • Korean Journal of Ichthyology
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    • v.13 no.1
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    • pp.74-84
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    • 2001
  • The early developmental stages, growth and morphological changes of the Korean bullhead, Pseudobagrus fulvidraco, were studied from a series of reared specimens. Details of the early developmental stages are illustrated with special reference to morphological transformations. Egg and sperm of Korean bullhead were obtained from mature adults under hormonal treatment, fertilized artificially, and incubated in the aquarium. The incubation period of fertilized eggs was 55 to 66 hours at a temperature of 24.9${\pm}$0.34$^{\circ}$. Larvae were fed successively with Artemia salina and Daphnia magna for 2 to 15 days and artificial food after 20 days. Fertilized eggs were adhesive and spherical with a diameter of 2.04mm(n = 100). The mean total length of newly hatched larvae was about 4.92${\pm}$0.33 mm. Mouth opening occurred on one-day-old yolk-sac larvae, and initial feeding was observed on the third day after hatching. The morphological transitions from larvae to juvenile and juvenile to young stages occurred when the fish reached about 17 mm in total length (about 13days after hatching) and about 32 mm in total length (about 30 days after hatching), respectively. Many changes in proportion of body parts to total length were observed at about 7~8 mm and 30~32 mm, corresponding to the transformations from larvae to juvenile and from juvenile to young, respectively. In comparing relative growth of each body part against total length, those characteristics related to head parts showed positive growth in the pre-larval stages, while those concerning mobile abilities showed positive growth in the post-larval stage.

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Parturition and Morphological Development of Larvae and Juvenile in Hippocampus kuda from Korea

  • Lee, Gi-Seok;Lee, Sung-Hun;Lee, Jin;Park, Jea-Min;Han, Kyeong-Ho
    • Development and Reproduction
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    • v.22 no.4
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    • pp.361-367
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    • 2018
  • The newborn, Hippocampus kuda larvae were 6.97-8.81, standard length (SL) mm (mean 7.89 mm) and mouth and anuse were open. Dorsal fin rays 15-18 and pectoral fin-rays were counted 8 and had 10-11+21 myotomes, body's bony plate ring being developed strongly in the central axis of myotomes part. 4 days after bearing, the SL was 7.02-9.47 mm (mean 8.24 mm) and nostrils began to open. 12 days after bearing, larvae attained to 8.91-11.2 SL mm (mean 10.0 mm). From this time, their unique predation habit appeared. 21 days bearing, larvae attained to 12.1-14.8 SL mm (mean 13.4 mm) the and thorn of back was enlarged among the plate formed around ring. 41 days bearing, seahorses attained to 17.1-17.8 SL mm (mean 17.4 mm) and the number of body's bony plate ring of the top of rings trunk was 11 and on the tail of them was 33-36, similar to figure of adult.

Hemolymph Juvenile Hormone Binding Protein of Fifth Instar Larvae of Bombyx mori L.: Identification and Purification (누에나방의 5령유충 혈림프의 유약호르몬 결합단백질: 확인 및 정제)

  • Park, Chul-Ho;Kim, Hak-Ryul
    • The Korean Journal of Zoology
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    • v.37 no.1
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    • pp.66-75
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    • 1994
  • Juvenile hormone binding protein was identified in the hemolymph of fifth instar larvae and purified using column chromatography. Hemolymph was mixed with [3H] JH-III and electrophoresed on 691 NON-SDS gel, indicating that radioactivity peak appears at Rf value of 0.55. Gel filtration showed two radioactivity peaks equivalent to bound and free [3H]JH-III, respectively. JHBP was purified from hemolymph through gel filtration (Sephadex G-100), anion exchange chromatosraphv (DEAE Sepharose CL-6B), chromatofocusing chromatographv (PBE 94) and preparative electrophoresis.

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Morphological Development of Eggs, Larvae and Juveniles of the Far Eastern Catfish, Silurus asotus in Korea (Pisces: Siluridae)

  • Mun, Seong Jun;Yim, Hu Sun;Han, Kyeong Ho;Park, Jae Min
    • Development and Reproduction
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    • v.21 no.4
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    • pp.399-406
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    • 2017
  • This study was conducted to investigate egg development and larvae morphological development of catfish and to provide basic data to clarify the genetic relationship with Siluriformes fish. The mother fish that was used in this study was caught in the stream of Nakdong River in Uiseong-gun, Gyeongbuk. The temperature range of the breeding was $23.0-25.0^{\circ}C$ (mean $24.0{\pm}1.0^{\circ}C$) and egg size was 1.62-1.70 mm (mean $1.66{\pm}0.05$, n=30). Eggs of catfish began hatching at 54 hours and 40 minutes after fertilization. Immediately after hatching, the total length of larvae was 3.60-3.65 mm (mean $3.62{\pm}0.03$, n=5) and had an egg yolk without swimming ability. On the third day after hatching, the larvae at the medium stage was 8.00-8.65 mm (mean $8.32{\pm}0.45$) in total length, and two pairs of whiskers formed around the mouth were elongated. On the 12th day after hatching, the larvae at the juvenile stage was 16.5-17.0 mm (mean $16.7{\pm}0.35$) in total length, and the stem of each fin was in the range, and the juvenile at this period was morphologically similar to the mother fish.

Development of Larvae and Juveniles of the Smoothskin Scuplin, Ocynectes modestus (Ocynectes modestus (민가시꺽정이: 국명신칭) 자치어(仔稚漁)의 형태발달(形態發達))

  • Kim, Yong-Uk;Kang, Chung-Bae;Han, Kyeong-Ho
    • Korean Journal of Ichthyology
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    • v.12 no.2
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    • pp.146-153
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    • 2000
  • The development of larvae and juvenile of the smoothskin sculpin (Ocynectes modestus) caught at Pusan brook in May and June 1995 was described. Yolk was completely absorbed when the larvae was about 6.28 mm in average standard length (ASL) with 32~33 myomeres. Flexion of the notochord started when the juvenile was about 7.36 mm in ASL and finished about 9.34 mm in ASL, respectively. Aggregate numbers of all fin rays were completed at over 10.08 mm in ASL, when the larvae reached the juvenile stage. One pairs of flaps were observed on the dorsal surface of the head at 10.08 mm in ASL. The juveniles longer then 14.00 mm in ASL has the same pigment pattern as that of adults. A new Korean name "Min-Ga-Si-Ggeog-Jeong-i" is proposed for this species.

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Development and growth in fertilized eggs and larvae of the Japanese geoduck, Panopea japonica reared in the laboratory (코끼리조개, Panopea japonica (A. Adams)의 수정란 발생과 유생 성장)

  • Nam, Myung-Mo;Lee, Chu;Kim, MeeKyung;Kim, Jae Won;Kim, Young Dae
    • The Korean Journal of Malacology
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    • v.30 no.4
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    • pp.303-309
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    • 2014
  • The development of Japanese geoduck, Panopea japonica, grown under culture conditions, has been examined through the morphological characteristics in fertilized egg, larvae and juvenile. Gametes were stripped from ripe broodstock and placed into two separate containers. Eggs were washed through a $40{\mu}m$ sieve and fertilized with dilute sperm solution. Developing larvae were maintained at $19{\pm}1^{\circ}C$. Fertilized eggs with $81.6{\mu}m$ diameter developed to trochophores within 14 h and to D-stage larvae ($116{\mu}m$ shell length) within 27 h. Larvae were spontaneously settled at shell length of $311{\mu}m$ after 20 days. The hatching from fertilized eggs and larval rearing were normally available in $18.5-21.5^{\circ}C$, and the growth was good in a cashmilon substrate, as well as sand. After rearing of day 108 from metamorphosis, the shell length of juvenile P. japonica reached 13 mm, and growth rate of shell length of the juvenile was $117.5{\mu}m/d$.

Morphological Development of Egg, Larvae and Juvenile in Korean shinner, Coreoleuciscus splendidus from the Ungcheon-Stream of Korea

  • Park, Jae-Min;Cho, Seong-Jang;Jo, Hye-In;Han, Kyeong-Ho
    • Development and Reproduction
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    • v.23 no.2
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    • pp.161-170
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    • 2019
  • The purpose of this study is to investigate the development of Coreoleuciscus splendidus egg and larvae morphology in the Ungcheon-stream. C. splendidus eggs were round and ranged in size from 1.86-2.01 mm (mean $1.91{\pm}0.14mm$). Immediately after hatching, the larvae had egg yolk in the abdomen with a total length of 5.27-6.63 mm (mean $5.95{\pm}0.96mm$). On the 10 days after hatching, the latter was 8.44-8.65 mm (mean $8.54{\pm}0.14mm$) in total length, and 5 dorsal fin rays were formed in dorsal fin and 8 caudal fin soft rays were formed in caudal fin. At 88 days after hatching, total length was 26.2-25.7 mm (mean $25.9{\pm}0.35mm$), and scales were formed throughout the body. The fin rays of each part were iii.7 in dorsal fin and iii.6 in anal fin, and finally the external form was the same as the adult fish.

Development of Larvae and Juveniles and Growth Characteristics of the Sebastes taczanowskii (탁자볼락(Sebastes taczanowskii) 자치어의 형태발달 및 성장특성)

  • Seong-Jun Moon;Jin-Gak Kim;Yoon-Ha Kim;Ye-Sol Kwon;Seong-Min Yoon;Jae-Min Park
    • Korean Journal of Ichthyology
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    • v.35 no.1
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    • pp.1-9
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    • 2023
  • The morphological development of larvae and juvenile of Sebastes taczanowskii were studied. The S. taczanowskii were caught at Goseong-gun, Gangwon-do from March in 2016. Larvae beared at water temperature of 11~12℃ (mean 11.5±0.5℃). The just beared larvae were 5.25~5.96 mm (mean 5.60±0.19 mm, n=30) in total length and their mouth and anus were already opened. They began to eat rotifer and transformed to postlarvae stage. 35 days after bearing postlarvae was measured 7.16~11.4 mm (mean 9.02±1.00 mm) in total length and the tip of notochord was bent upward. 48 days after bearing juvenile was measured 9.45~18.7 mm (mean 14.4±1.75 mm) in total length with dorsal fin rays XIII, 13~15; anal fin rays III, 6~8; caudal fin rays 16.