• Proceedings of the Korean Society of Plant Pathology Conference
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• 1994.06a
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• pp.27-49
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• 1994

Root colonization of biocontrol agents via seed treatment was investigated and a compatible combination, Gliocladium virens G872B and Pseudomonas putida Pf3, in colonizing cucumber rhizosphere was confirmed through the study. Much higher number of fungal and bacterial propagules were detected when two isolates were inoculated together. The presence of Pf3 in root system was greatly helpful to G872B to colonize at root tip. The mechanism of this phenomenon is partially elucidated through the results of in vitro experiments and the observations of scanning electron and fluorescence microscope. Addition of Pf3 cells resulted earlier germination of G872B conidia and increased mycelial growth. And the more number of germinated conidia on seed coat, the more vigorous hypal streching and sporulation on the root surface were observed in coinoculated treatment. The propagules of G872B on the cucumber root when they were challenged against the pathogenic Fusarium oxysporum, were even higher than that of G872B treated alone, and the magnitude of such a difference was getting grater toward the root ip and the population of F. oxysporum on the root was reduced by seed inoculation of G872B. The rhizosphere competence was obviously reflected to disease suppression and plant growth promotion that induced by the given isolate. Green house experiments revealed that the combined treatment provided long-term disease suppression with greater rate and the larger amount of fruit yield than single treatments. Through this study the low temperature growing Pseudomonas fluorescens M45 and MC07 were evaluated to apply them to the winter crops in field or plastic film house. In vitro tests reveal that M45 and MC07 inhibited the mycelial growth of Pythium ultimum, Rhizoctona solani and Phytophthora capsici and enhanced growth of cucumber cotyledon in MS agar. This effect was more pronounced when the bacteria were incubated at 14$^{\circ}C$ than at 27$^{\circ}C$. And disease suppression and plant growth promotion in green house were also superior at low temperature condition. Seed treatment of M45 or soil treatment of MC07 brought successful control of damping-off and enhanced seedling growth of cucumber. The combined treatment of two isolates was more effective than any single treatment.

• Journal of Bio-Environment Control
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• v.23 no.2
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• pp.77-82
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• 2014

The present study was conducted to establish an effect and a proper concentration for treatment with gibberellic acid ($GA_3$) and thidiazuron (TDZ), resulting with increase berry size and yield in Gaeryangmeoru grapes. Berry size was increased by treatment with $GA_3$, and the fruit clusters obtained for the groups treated with $GA_3$ concentrations of 100 and $200mg{\cdot}L^{-1}$ were bigger. The berry number was also enhanced in $GA_3$ treated groups, but the soluble solid content and acidity was not significantly different. Damage caused by $GA_3$ treatment, such as peel pollination and berry shatter, was observed in the group with $200mg{\cdot}L^{-1}$. The berry size was larger in group treated with a high concentration of $GA_3$ and TDZ respectively than in those treated with low concentrations in the treatment mixed $GA_3$ and TDZ; however, fruit with low soluble solid content and high acidity was harvested after $GA_3$ and TDZ treatment due to delay of berry ripening. The pericarp tissue layers were not changed, but the distance from the epidermis layer to vascular bundle tissue was increased as a result of $GA_3$ and TDZ treatment. Therefore, $GA_3$ and TDZ did not affect an cell division but not cell size, resulting in an enlarged berry size. It is necessary to treat plant growth regulators 2~3 times and immediately after berry set to enhance berry set rate, because the period of berry set is short. This study suggests that the proper concentration for enhancing berry size and set were up to $100mg{\cdot}L^1$ $GA_3$ or $50mg{\cdot}L^{-1}GA_3+1.25mg{\cdot}L^{-1}$ TDZ, and it is necessary to pay attention to harvest mature fruits because of the delay of ripening caused by the usage of TDZ.

• Korean Journal of Food Science and Technology
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• v.33 no.2
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• pp.200-204
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• 2001

Persimmon (Diospyros kaki. cv. Fuyu) fruits were packaged under different conditions, and then stored at $0^{\circ}C$ for 21 weeks. The tried packages were heat-sealed bag of one fruit, heat-sealed bag of five fruits, and tie-sealed bag of five fruits, which used films of three different thickness (0.03 ㎜, 0.05 ㎜ and 0.06 ㎜). Throughout the storage, package free volume, package atmosphere and quality were measured. Package free volume decreased with time with higher rate for heat-sealed bags, in which close contact between fruit and the film was eventually reached in longer storage. However, tie-sealed bags maintained the levels of stabilized free volume. The rate of free volume decrease was faster with thinner film and larger bag size for the packs sealed by the same method. Package atmosphere covered $O_2$ concentration of $1.1{\sim}17.1%$ and $CO_2$ concentration of $1.1{\sim}8.3%$, $O_2$ concentration decreased during storage, whereas $CO_2$ increased. Thinner film package created the internal atmosphere of higher $O_2$ and lower $CO_2$ concentrations. Tie-sealed bags of 5 fruits in the films of 0.05 mm and 0.06 mm thickness maintained the equilibrated package atmosphere of $1.1{\sim}3.0%\;O_2$ and $4.0{\sim}5.5%\;CO_2$, which preserved the fruits best in terms of firmness and less physiological changes of flesh browning and peel browning. At five fruit heat-sealed bag in the films of 0.06 mm thickness with $CO_2\;8.3%$, flesh browning occurred at a high rate, whereas all treatments in the film of 0.03 mm thickness with high $O_2$ and relatively low $CO_2$ contents, flesh and peel browning rates were high.

• Journal of Bio-Environment Control
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• v.9 no.2
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• pp.85-93
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• 2000

This study was carried out to investigate the effects of several substrates and ratio of NO3--N to NH4--N in nutrient solution on growth, yield and mineral uptake of sweet pepper(Capsicum annuum L.) in bag culture. The content of minerals such as P, K, Ca and Mg showed difference in concentration among media: P and Mg were the highest in vermiculite in vermiculti+rice hull, K in perlite+vermiculite and Ca in perlite+peatmoss; with the lowest in the single perlite medium respectively, Ca of mineral made fairly higest level in concentration compared with the others in all of the media. The concentration of mineral content was lower in the ratio of 8:2 than that of 10:0. Admitting that the pH made difference depending on the kind of substrates and ratios of NO3--N to NH4--N. The pH of 10:0 ratio in all the substrates was higher and more stable than that of 8:2. The range of EC in all the substrates showed from 1.78ds·m-1 to 2.10 ds·m-1, which was optimum range for growth of sweet pepper, and range of EC is larger in 8:2 ratio than that in 10:0 ratio. Plant height and stem diameter were nothing to do with the kind of substrates, but leaf area was the largest at vermiculite+rice hull of the 8:2 ratio, fresh and dry weights were heavier at peatmoss+carbonized rice hull, but were the lightest at perlite. All indexes related to the growth which had something to do with the kind of substrates higher in 8:2 ratio than those in 10:0 ratio. The number of fruit and fresh weight related to the ratio of the 8:2 were the highest as 17.5 at vermiculite+rice hull with 1,588g of fresh weight, while the yield from perlite was the lowest. The number of fruit was the highest as 16.4 at virmiculite+rice hull, yield was the higest as 1,394a at perlite+ peatmoss. The yield of 8:2 ratio at all substrates was higher than that of 10:0 ratio. Of the mineral content related to the plant part, K+ and Mg2+ were higher in concentration at leaf; Ca2+ were higher at root; PO4- was higher at stem and fruit; The content of mineral showed no difference between the ratio of the 8:2 and the ratio of the 10:0 with no regrading to the difference of mineral content among substrates; and K+, Ca2+, and Mg2+ uptake of sweet pepper were higher at 10:0 ratio than that of 8:2; 2while PO4- uptake of sweet pepper was lower at 10:0 ratio than that of 8:2 ratio.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.226-233
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• 2005

This study was carried out to evaluate the fruit and growth characteristics of selecting Vitis amurensis through functional material analysis and sensory evaluation in V. amurensis collected in Gangwondo. For evaluation of growth characteristics in V. amurensis, experiments were carried out by compared with the two grape cultivars 'Campbell Early' and 'Kyoho'. The full bloom and verasion time in V. amurensis was investigated faster than those of examined cultivar grapes, while harvest time was investigated latter than those of examined cultivar grapes, but agronomic characteristics was not thought significantly difference between cultivar grapes and V. amurensis. For evaluation of shoot growth phase, the growth curve was very similar to cultivar grapes. The berry size in V. amurensis showed that increases rapidly between 3 and 4 days after full bloom time, and approximately doubles between the second growth period and harvest time, and the berry development phase investigated that consist of two sigmoid growth periods separated by a lag phase. The berry weight and soluble solids in V. amurensis increased with the tree age, but acidity and total sugar contents decreased, and showed a special quality and stable growth according to vine age. To investigation of functional materials, the anthocyanin content in V. amurensis ranged from 16.6 to 50.2 mg/100 g, and the resveratrol content ranged from 0.143 to $0.236{\mu}g/100\;g$ which was higher than those of cultivar grapes. These result indicated that V. amurensis tended to have the useful material larger than cultivar grapes. Therefore, other edibility factors of V. amurensis collected in Gangwondo may contribute to breeding studies in Vitis. spp.

• Korean Journal of Food Science and Technology
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• v.6 no.2
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• pp.98-108
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• 1974

Fourteen varieties of Korean chestnut were subjected to the test of adaptability for processing and the possibilities of colored products development. The fruit size of Korean chestnut ranged $11{\sim}18g$ and these small fruits could not be expected to be utilized for the processing of Marron glaces which requires larger size as $25{\sim}30g$. As the storge period is extended the discoloration tendency of chestnut flesh was increasingly developed, however, the fresh chestnut has shown layer-separating phenomenon and ragged surface of fruit which delivers disagreeable appearance to the finished product. The principal factors of discoloration occurred during processing were the behavior of tannin and darkening rate shown on flesh differed each other among varieties; the Chukpa and Yuma variety have exhibited the most serious discoloration and the Taab-b variety, the lightest. Taab-b variety in this connection could be expected to be available for Kanroni processing. For the industrialization of chestnut processing the flame-scorched peeling method is advisable. The capacity of this method is proportional to the square of scorching radius and highly flexible in controlling its performance. As for the processing of colored product, the sugar dehydration and coating and the sugar penetration method demand further study in basical views; however, the canned product of chestnut-redbean has shown the possibility of being utilized as a substitute for or paralleled use with the sugar-syruped canned product which so far has been considered as the only item of export to Japan.

• Journal of Bio-Environment Control
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• v.3 no.2
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• pp.119-127
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• 1994

This study was conducted to investigate the effects of temperature and light conditions on the vigor and growth responses of red pepper(Capsicum annuum L.) seedlings in the nonheated plastic houses with triple and quadruple coverings during winter. The results obtained were as follows ; 1. The growth in terms of plant height, number of leaves, leaf area and dry weight of each organ was reduced up to 50% in the triple coverings compared to quadruple ones. The greatest difference between the triple coverings and quadruple ones was the leaf area, showing two to five times larger in the quadruple ones. Therefore, the differences of the early environmental conditions during raising seedling stage was well reflected. 2. The leaf expansion in the early stage of seedling was delayed in the triple coverings and subsequently crop growth rate(CGR) significantly reduced. The specific leaf area (SLA) in the triple coverings was less than quadruple ones. The net assimilation rate (NAR) was increased in the early stage of seedlings and then reduced in the quadruple coverings. In triple ones, however, the great reduction showed at six weeks after sowing and then increased sharply. 3. The linear relationship among leaf area, total dry weight and leaf dry weight was observed in the all experimental areas, while between leaf area and crop growth rate only in the quadruple coverings, and between leaf area and net assimilation rate in both triple and quadruple ones. 4. The dry matter partitioned to the leaf increased up to six weeks after sowing in the triple coverings but reduced in the stem and root. However, the adverse results were obtained in the quadruple ones. 5. In the triple coverings, the number of branches showed the exponential increment while fruit weight showed linear increment in both triple and quadruple ones. Higher yield was obtained in the quadruple ones. 6. The shoot dry weight among the characteristics of seedlings was greatly contributed to the yield of red pepper after transplanting. Accordingly, one of the decisive criterion for good quality of red pepper seedlings was determined by the highest correlation between shoot dry weight of growing plant and fruit yield of pepper plant.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.598-603
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• 2016

The aim of this study was to elucidate the influence of vigor of a bearing shoot in ‘Bluecrop’ Highbush Blueberry (Vaccinium corymbosum L.) on growth characteristics of shoots and fruits. Bearing shoots were classified with BS (bearing shoot) and BMB (bearing mother branch). The vigor of bearing shoots were divided into four arbitrary categories; A was thin (< 6.0 ㎜) BMB and short (< 10 ㎝) BS, B was thin BMB and long (≥ 10 ㎝) BS, C was thick (≥ 6 ㎜) BMB and short BS and D was thick BMB and long BS. Shoots from D were longer (6.5 ㎝) and thicker (1.70 ㎜) than those from the others. Shoots of D had more leaves (5.8 ea) than those of the others. Leaf area of D was larger (13.5 ㎠) than those of the others. The first harvest of D was one week faster than the others. Ratio of big berry (>14 ㎜) from the long BSs was higher (B : 41.7, D : 46.8%) than that from the short BSs. Soluble solid content of small berrys did not show any different according to vigor of bearing shoots, but soluble solid content of big berrys of the long BSs was higher (B : 16.2, D : 15.6°Bx) than those of the short BSs. The thickness of BMB did not affect ratio of fruit size and soluble soild content. The long BSs would be proper than the short BSs for bearing bigger fruits.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.75-85
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• 2012

This study was investigated to develop an alternative medium for the cultivation of oyster mushrooms. The cultivation characteristics for major cultivated varieties of oyster mushrooms were campared with the food waste medium. In addition, water control & a gap formation of food waste is being pointed out as a problem of the medium material. Therefore, certain amounts of earthworm casting were supplied to the food waste medium to overcome the water and compaction phenomenon. This study investigated the use of the total 6 cultivars of oyster mushrooms, the Suhan(PL.1), Chiak No. 5(PL.2), Chiak No. 7(PL.3), Samgu No. 5(PL.4), Samgu No. 8(PL.5), Jangan No. 5(PL.6). In terms of medium, the optimum growth of the mycelium of the oyster mushroom was observed in the beet pulp medium but in food waste, the mycelium's growth was observed as insufficient in the bulk of cultivars. The Jangan No. 5(PL.6) was superior to other cultivars relative to the mycelium's growth. The optimum concentration of earthworm casting added the foodwaste medium was 30%. When Jangan No. 5(PL.6) was incubated in the foodwaste meidum containing 30% earthworm casting, the early days to germination were long, almost double the amount of time than that of the beet pulp medium. However, it was found that the fruit body size was larger than that of the beet pulp by up to 20.5%, and the length of the fruit body was up to 6.2% longer than that of the beet plup. The total yields increased by 60.5%. The analysis results of the oyster mushroom's nutritional contents cultured using the food waste medium, shows that the protein, vitamin A and vitamin E increased 70.6%, 2.4%, 0.8% respectively, the fat decreased by 12.6% and the oleic acid increased in a very small amount compared to that of the beet pulp medium.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.706-713
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• 2013

With pot-grown 4-year-old 'Fuyu' persimmon trees, this study evaluated the effect of different nitrogen (N) rates during summer on fruit characteristics, changes of leaf nutrients after harvest, reserve accumulation, and early growth the following year. A total of 0, 36 g N in June, and 72 g N in June and July was fertigated to each tree using urea solution. All the fruits were harvested on Nov. 3. Although not significant, fruits were larger for the 36 g and 72 g N than the 0 g N. Fruits for the 0 g N, having lower N concentration, were softer and had a better coloration and higher soluble solids, indicating that they matured earlier. SPAD value on Nov. 3 was 19.2 for the 0 g N and 54.9 for the 72 g N, and then the values linearly decreased in all the treatments by Nov. 14, exhibiting rapid leaf senescence. Specific leaf weight, being the lowest for the 0 g N, also gradually decreased during this period. Increasing N level significantly increased cross-sectional area of the trunk. Leaf N concentration on Nov. 3 was 0.87% for the 0 g N, whereas it was 1.18 and 1.52% for the 36 g and 72 g N, respectively. The N fertigation tended to increase leaf concentrations of soluble sugars, starch, and amino acids. Contents of N, P, K, soluble sugars, starch, and amino acids per unit leaf area gradually decreased in all the treatments during the 11 days after harvest, and the extent of the decrease was the lowest for the 0 g N. On the other hand, those of Ca, Mg, and protein did not consistently change during this period. The N fertigation resulted in higher concentrations of N in dormant shoots on Nov. 14, and although not great, it also increased soluble sugars, starch, amino acids, and protein. Clear differences were found in number of flower buds per one-year-old branch and total shoot length per tree the following year. The 72 g N trees had 5.6-fold more flower buds and 1.9-fold more shoot length, compared with those of 0 g N trees. However, it was noted that tree growth the following year was not significantly different between the 36 g and 72 g N the previous year. It was concluded that N rate during summer should be adjusted with considering the changes of fruit maturation, mobilization of leaf nutrients, and reserve accumulation.