• Animal Bioscience
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• 제35권11호
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• pp.1649-1655
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• 2022

Objective: The primary objective of this study was to determine the genetic parameters for reproductive traits among Large White pigs, including the following traits: total number born (TNB), number born alive (NBA), litter birth weight (LBW), average birth weight (ABW), gestation length (GL), age at first service (AFS) and age at first farrowing (AFF). Methods: The dataset consisted of 19,036 reproductive records from 4,986 sows, and a multi-trait animal model was used to estimate genetic variance components of seven reproductive traits. Results: The heritability estimates for these reproductive traits ranged from 0.09 to 0.26, with the highest heritability for GL and AFF, and the lowest heritability for NBA. The repeatabilities for TNB, NBA, LWB, ABW, and GL were ranged from 0.16 to 0.34. Genetic and phenotypic correlations ranged from -0.41 to 0.99, and -0.34 to 0.98, respectively. In particular, the correlations between TNB, NBA and LBW, between AFS and AFF, exhibited a strong positive correlation. Furthermore, for TNB, NBA, LBW, ABW, and GL, genetic correlations of the same trait between different parities were moderately to strongly correlated (0.32 to 0.97), and the correlations of adjacent parities were higher than those of nonadjacent parities. Conclusion: All the results in the present study can be used as a basis for the genetic assessment of the target population. In the formulation of dam line selection index, AFS or AFF can be considered to combine with TNB in a multiple trait swine breeding value estimation system. Moreover, breeders are encouraged to increase the proportion of sows at parity 3-5 and reinforce the management of sows at parity 1 and parity ≥8.

• Asian-Australasian Journal of Animal Sciences
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• 제33권1호
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• pp.132-138
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• 2020

Objective: To investigate the effects of low-dose trace mineral proteinates on reproductive performance, mineral status, milk immunoglobulin contents and fecal mineral excretion of sows. Methods: Eighty crossbred sows (Landrace×Large White) were randomly allocated to two groups in a 135-day trail, from breeding through 21 d postpartum. The two treatments were inorganic trace minerals (ITM): a basal diet+inorganic iron (Fe), copper (Cu), manganese (Mn), and zinc (Zn) at 90, 15, 25 and 90 mg/kg, respectively and organic trace minerals (OTM): a basal diet+proteinates of Fe, Cu, Mn, and Zn at 72, 12, 20, and 72 mg/kg, respectively. Results: Compared with ITM, OTM significantly increased (p<0.05) the number of piglets with birthweight >1 kg, the litter weaning weight, and milk Fe, Cu contents. No significant differences (p>0.05) were observed on sow hair mineral contents or immunoglobulin M (IgM), IgG, and IgA contents in colostrum and milk. In comparsion to ITM, OTM decreased fecal Fe, Cu, Mn, and Zn contents of gestating sows (p<0.01) and Fe, Mn, and Zn in lactating sows (p<0.01). Conclusion: These results indicate that low-dose mineral proteinates can increase the number of piglets with birthweight >1 kg, the litter weaning weight and certain milk mineral concentrations while reducing fecal mineral excretion.

• Reproductive and Developmental Biology
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• 제39권1호
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• pp.23-28
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• 2015

본 연구는 번식돈 농장의 경영지표 설정에 필요한 기초적 자료를 얻기 위하여 충남지역에서 사육하고 있는 번식모돈 소규모 4개 농장, 중규모 5개 농장, 대규모 6개 농장(총 15개 농장)을 대상으로 1년간의 모돈의 전산경영기록 자료중 번식 및 육성성적을 조사하여 분석하였다. 평균 상시모돈수는 모돈 사육규모별 간에는 유의한 차이(p<0.05)를 보였는데, 소규모 농장은 114.25두와 대규모 농장은 489.88두로 소규모 농장은 가족노동관리인 반면에, 대규모 농장은 고용인력을 활용해야 할 것으로 사료된다. 모돈규모가 클수록 분만율이 다소 높은 경향을 보였으나 규모별간에는 유의한 차이는 없었다. 복당평균산자수는 모돈규모별 간에는 유의한 차이는 없지만 대규모 농장보다는 소규모 농장에서 총산자수가 점점 큰 경향을 보였다. 포유개시두수는 모돈규모별 간에는 유의한 차이(p<0.05)가 인정되었는데, 중규모나 대규모 농장(10.28두)보다는 소규모 농장에서 10.48두로 포유개시산자수가 가장 많은 것으로 나타났다. 복당 평균이유두수는 모돈농장 규모 간에는 통계적 유의차(p<0.05)를 보였는데, 소규모 농장에서 8.89두로 가장 낮은 성적이었으나, 대규모 농장에서 9.35두로 모돈규모가 클수록 이유두수가 양호한 것으로 나타났다. 복당 이유일령은 농장규모별 간에는 유의한 차이(p<0.05)를 보였는데, 중규모농장에서 평균 23.79일로 이유일령이 가장 빠른 반면에, 소규모 농장에서는 평균 27.26일로 가장 늦게 이유시키고 있었다. 평균모돈 회전율은 모돈규모별 간에는 소규모나 대규모 농장보다 중규모 농장집단이 평균 2.32회로 유의성 있게(p<0.05) 가장 높은 모돈회전율을 보였다. 자돈평균육성율은 소규모 농장(87.13%)와 대규모 농장(91.69%) 집단보다는 중규모잡단에서 94.70%로 가장 양호한 성적을 보였다. 평균 출하 체중은 3개 농장 집단규모 간에는 유의한 차이는 없지만, 소규모나 중규모보다는 대규모 농장에서 112.37 kg으로 모돈농장 규모가 클수록 다소 높게 출하하는 경향으로 나타났다. 분만율과 산자수, 포유개시두수, 이유두수, 모돈회전율 간에는 유의한 정(正)의 상관관계, 그리고 총산자수와 포유개시두수 간에는 정의상관이 있었으나, 총산자수와 자돈육성율 간에는 부(負)의 상관이 존재하였다. 포유개시두수는 이유두수와 이유일령 간에는 정의 상관이었으나, 포유개시두수와 육성율 간에는 유의한 부의 상관이 있었다. 이유두수와 모돈회전율 및 육성율 간에는 각각 정의 상관이었으나, 이유일령과 모돈회전율 및 육성율 간에는 각각 유의한 부의 상관이 존재하였다. 소규모 모돈농장에서는 산자능력이 우수한 반면에 중규모와 대규모 농장에서는 각각 육성율과 이유두수 성적에서 비교적 우수한 것으로 평가되었다. 그러나 전반적으로 분만율 향상을 위한 기술력 확보가 요구되었다.

• The Plant Pathology Journal
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• 제16권4호
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• pp.236-241
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• 2000

A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

• Journal of Ecology and Environment
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• 제38권1호
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• pp.75-84
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• 2015

Tropical forests play a critical role in mitigating climate change, and therefore, an accurate and precise estimation of tropical forest carbon (C) is needed. However, there are many uncertainties associated with C stock estimation in a tropical forest, mainly due to its large variations in biomass. Hence, we quantified C stocks in an intact lowland mixed dipterocarp forest (MDF) in Brunei, and investigated variations in biomass and topography. Tree, deadwood, and soil C stocks were estimated by using the allometric equation method, the line intersect method, and the sampling method, respectively. Understory vegetation and litter were also sampled. We then analyzed spatial variations in tree and deadwood biomass in relation to topography. The total C stock was 321.4 Mg C $ha^{-1}$, and living biomass, dead organic matter, and soil C stocks accounted for 67%, 11%, and 23%, respectively, of the total. The results reveal that there was a relatively high C stock, even compared to other tropical forests, and that there was no significant relationship between biomass and topography. Our results provide useful reference data and a greater understanding of biomass variations in lowland MDFs, which could be used for greenhouse gas emission-reduction projects.

• Asian-Australasian Journal of Animal Sciences
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• 제12권6호
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• pp.939-943
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• 1999

Three 28 day-old $Duroc{\times}Large$ $White{\times}Landrace$ litter mate gilts weighing an average of 6.5 kg were used to study the intestinal absorption of amino acids when provided in dipeptide form or in the form of a free amino acid mixture. The pigs were given one of three treatments. The control involved a duodenal infusion containing no amino-acids (phosphate buffer plus 5% sorbitol) while the remaining two treatments involved either a duodenal infusion containing a glycine-lysine dipeptide (1 g) or a mixture of the free amino acids glycine and lysine at the same concentration as in the dipeptide. Blood was drawn from a cannula inserted in the portal vein, at 5 to 20 minute intervals, for two hours following infusion. The concentration of intact dipeptide as well as free glycine and lysine in the portal blood was determined by high performance liquid chromatography. The intact dipeptide was never detected in the portal blood at any time after infusion. Lysine appeared in the portal blood more rapidly after infusion of dipeptide than after infusion of free lysine and the concentration of lysine in portal blood was higher in the pig infused with the dipeptide than after infusion of free lysine at almost all time points measured. The cumulative absorption of lysine and glycine from the intestine during the two hour period after infusion was greater in the pig infused with dipeptide than in the pig infused with free amino acids. The results suggest that although intact dipeptide did not reach he portal circulation, a special transport mechanism for absorption of dipeptide by intestinal cells appears to be present in pigs similar to that observed in other species.

• Asian-Australasian Journal of Animal Sciences
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• 제13권7호
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• pp.888-893
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• 2000

The present study was conducted to evaluate heterosis effects of body weight and jumping height for successive generations of rotational crossing using two subspecies of mice which are very different in body weight and in genetic relationship from each other. Domesticated laboratory mouse $CF_{{\sharp}1}$ (C) and Yonakuni wild mouse (Y) were used as materials. Two groups of rotational crossing was made according to the parent used at the beginning of crosses, C male$\times$Y female and Y male$\times$C female. These crosses were done to produce the first ($G_1$ and $G_1{^{\prime}}$), second ($G_2$ and $G_2{^{\prime}}$) and third generations ($G_3$ and $G_3{^{\prime}}$) with sire used was alternated. Individual body weights were weighed at 1 (wk1), 3 (wk3), 6 (wk6) and 10 weeks of age (wk10) and jumping heights were measured at six weeks of age (wk6). Only the first litter used. For body weight, results of this study showed that genetic group effects were significant (p<0.01) source of variation at all ages studied. Sex effects were significant (p<0.01) at wk3, wk6 and wk10, but not at wk1. Significant interaction effects (p<0.01) between genetic group and sex were found at wk6 and wk10. The C mice with large maternal effects produced heavier offspring body weight and crosses using sire of this subspecies maintained heavy weight compared to wild Y mouse sire that has small body size. Heterosis tended to exist at the rotational crossing started from Y male C female. For jumping height, effects of genetic group and sex were significant, sire and dam effects (heterosis) exhibited from the first to third generations, and no maternal effects were observed.

• Asian-Australasian Journal of Animal Sciences
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• 제26권3호
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• pp.343-348
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• 2013

This experiment was conducted to evaluate the development of T cells in intrauterine growth retarded (IUGR) piglets at different gestational stages, and tentatively explore the relationship between T cells development and the Notch signaling pathway. A total of 18 crossbred (Landrace${\times}$Large white) primiparous sows were mated at similar weights and estruses and euthanized at d 60, 90 and 110 of gestation with six replicates for each time point. One IUGR and one normal fetus were picked from each litter. The T-cell subsets, mRNA expression of Delta-like1, Delta-like4, Jagged1, and Notch2 genes in the thymus were investigated. Compared to normal piglets, $CD3^+CD4^-CD8^+$ cells in IUGR fetuses at d 90 was 0.13% lower (p<0.05). At d 110 of gestation $CD8^+$ T cells in IUGR fetuses was 0.19% lower (p<0.05). The percentage of $CD8^+$ T cells was 3.14% lower (p<0.05) of the total T cells in IUGR pigs at d 60. The abundance of Notch2 and Delta-like4 mRNA at d 110 was 20.93% higher and 0.77% (p<0.05) lower, and Delta-like1 mRNA at d 90 was 0.19% (p<0.05) higher compared to normal pigs. These results suggested that normal fetuses had a greater proportion of T-cell subsets at earlier gestation periods, and the Notch signaling pathway was likely partially responsible for these differences to some degree.

• Asian-Australasian Journal of Animal Sciences
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• 제24권8호
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• pp.1142-1147
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• 2011

Lysine intake during gestation has a major impact on subsequent reproductive performance. The objective of this experiment was to determine the effect of lysine intake from mid-gestation until farrowing on the reproductive performance of multiparous sows. On day 30 of gestation, 200 Landrace${\times}$Large White sows were randomly assigned to one of four groups based on body weight and parity (n = 50). The gestation diets contained 0.46, 0.56, 0.65 or 0.74% lysine. Increasing dietary lysine concentration improved sow body condition at farrowing and increased litter weights (p<0.05). Dietary lysine level also had a significant effect on the dry matter (p<0.05) and protein content (p<0.05) of colostrum. Increased lysine intake increased serum insulin concentration (p<0.05) and there was a trend towards increased serum prolactin content (linear, p = 0.07). However, increased lysine tended to decrease blood urea N (quadratic, p = 0.05). These results suggest that higher lysine levels (0.65-0.75%) than those recommended by the National Research Council improved reproductive performance for multiparous gestating sows and this increase may be partially mediated through blood metabolites or metabolic hormone levels.

• Asian-Australasian Journal of Animal Sciences
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• 제33권1호
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• pp.120-126
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• 2020

Objective: This study was conducted to determine the effects of dietary supplementation with Pediococcus acidilactici (P. acidilactici) ZPA017 as a probiotic on reproductive performance, fecal microbial flora and serum indices in sows during late gestation and lactation. Methods: A total of 94 sows (Large White×Yorkshire, average 4.50 parities) were randomly allotted to two dietary treatments: control diet and the diet supplemented with P. acidilactici ZPA017 (2.40×10⁹ colony-forming unit/kg of diets). The study started at d 90 of gestation and conducted until d 28 of lactation. Results: Compared to sows fed the control diet, supplementation of P. acidilactici ZPA017 increased the number of weaning piglets, weaning weight of litter and piglets, survival rate of piglets at weaning (p<0.05), and decreased diarrhea rate of piglets in lactation (p<0.05). Dietary P. acidilactici ZPA017 increased fecal Lactobacillus populations (p = 0.030) and reduced fecal Escherichia coli and Staphylococcus aureus populations (p<0.05) of sows at weaning. Moreover, the supplementation of P. acidilactici ZPA017 increased serum concentrations of immunoglobulin G, immunoglobulin A and total protein (p<0.05), while decreased serum haptoglobin concentration and alanine aminotransferase activity (p<0.05) of sows at weaning. Conclusion: Administration of P. acidilactici ZPA017 in diets during late gestation and lactation had positive effects on the reproductive performance, intestinal microflora balance and immunity of sows.