• 제목/요약/키워드: Large Quantity Production

검색결과 151건 처리시간 0.025초

집먼지에 서식하는 긴털가루진드기(진드기목: 가루진드기과)의 대량 사육방법 개발 (Development of mass rearing technique of Tyrophnn putrescentiae (Acari: Acaridae) found in house dust)

  • 이한일;이인용
    • Parasites, Hosts and Diseases
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    • 제35권3호
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    • pp.149-154
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    • 1997
  • 저장 곡류 해충으로 알려져 있는 긴털가루진드기(rophnwpkescentios)가 우리 나라 가옥 내 집먼지 서식 진드기류 중 제 3의 우점종으로 밝혀짐에 따라 알레르기성 질환을 야기하는 원인 항원으로 작용할 가능성이 높아져 연구의 대상이 되고 있다. 본 연구에서는 진단, 치료 및 면역학 적 연구에 필요한 다량의 항원을 공급하기 위하여 대량 사육방법의 개발을 시도하여 다음과 같은 결과를 얻었다. 긴털가루진드기를 대량 사육하는데 가장 적합한 사육배지로는 실험동물사료 분말 로 사육 10주 후 1,251.5배의 개체 증가를 보였고 효소분말과 실험동물 사료분말의 혼합배지 (1 : 1)에서도 사육 10주 후 1.203.1배의 개체 증가를 보여 거의 같은 성적을 보였다. 사육조건으 로 가장 적합한 온도와 상대습도는 $25^{\circ}C$. 64% RH로서 9주 후 960배로 가장 높은 증식결과를 보였고, $^25{\circ}C$와 75% RH에서도 935.4배의 개체 증가로 거의 동일한 결과를 나타냈다 사육용기 표면적을 $79{\;}cm^2,{\;}154{\;}cm^2{\;}및{\;}227{\;}cm^2$로 사육 비교한 결과 각각 860.8배. 924.6배 및 1,250.8 배의 개체 증가를 보여 현저한 차이는 보이지 않았다.

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집단에너지 사업자간의 열연계 메커니즘 구성에 의한 최적 열연계 산정 알고리즘 개발 (Development of Optimal Thermal Transfer Calculation Algorithm by Composition of Thermal Transfer Mechanism among Integrated Energy Operators)

  • 김용하;김승희;현승연
    • 에너지공학
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    • 제26권4호
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    • pp.57-66
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    • 2017
  • 열은 전력과 같이 이동속도가 빠르지 못하고 전력에 비해 손실이 비교적 크게 발생하므로 전력거래와 같이 한개의 운용센터를 두고 열 연계 시스템을 운용하는 것은 현실성이 없다. 현재 열 연계가 모두 이루어지고 있는 한국지역난방공사의 경우에도 인접한 2~4개 정도의 열병합발전소간에만 열 거래가 이루어지고 있는 실정이다. 따라서 본 논문에서는 열 거래를 위한 통합운용센터를 몇 개의 권역으로 나누어 각 권역마다 권역의 Hub 통합운용발전소를 두고 운용하는 것이 열매체의 특성을 반영하는데 적정하고 타당한 것으로 판단하여 집단에너지 사업자간 열 연계 메커니즘을 제안하였다. 제안된 메커니즘에 최적으로 열 거래를 행할 수 있는 알고리즘을 개발하고 이를 실제 사업자에 적용하여 제안한 알고리즘의 유용성을 검증하였다.

Optimization and High-level Expression of a Functional GST-tagged rHLT-B in Escherichia coli and GM1 Binding Ability of Purified rHLT-B

  • Ma Xingyuan;Zheng Wenyun;Wang Tianwen;Wei Dongzhi;Ma Yushu
    • Journal of Microbiology
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    • 제44권3호
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    • pp.293-300
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    • 2006
  • The Escherichia coli heat-labile enterotoxin B subunit (HLT-B) is one of the most powerful mucosal immunogens and known mucosal adjuvants. However, the induction of high levels of HLT-B expression in E. coli has proven a difficult proposition. Therefore, in this study, the HLT-B gene was cloned from pathogenic E. coli and expressed as a fusion protein with GST (glutathion S-transferase) in E. coli BL2l (DE3), in an attempt to harvest a large quantity of soluble HLT-B. The culture conditions, including the culture media used, temperature, pH and the presence of lactose as an inducer, were all optimized in order to obtain an increase in the expression of soluble GST-rHLT-B. The biological activity of the purified rHLT-B was assayed in a series of GMI-ELISA experiments. The findings of these trials indicated that the yield of soluble recombinant GST-rHLT-B could be increased by up to 3-fold, as compared with that seen prior to the optimization, and that lactose was a more efficient alternative inducer than IPTG. The production of rHLT-B, at 92 % purity, reached an optimal level of 96 mg/l in a 3.7 L fermentor. The specific GM1 binding ability of the purified rHLT-B was determined to be almost identical to that of standard CTB.

이산화탄소의 화학적 전환에 의한 폴리카보네이트 및 폴리우레탄의 원료 합성 (Synthesis of the Raw Materials of Polycarbonate and Polyurethane by CO2 Chemical Utilization)

  • 백준현
    • KEPCO Journal on Electric Power and Energy
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    • 제2권2호
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    • pp.187-192
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    • 2016
  • 이산화탄소의 화학적 전환기술은 온실가스 저감뿐만 아니라 탄소자원화를 통해 유한한 자원을 대체할 수 있는 기술이다. 다양한 화학반응에 의한 이산화탄소의 전환이 상용화되어 있지만, 대량의 이산화탄소를 자원화하기 위해서는 혁신적인 기술개발이 필요하며 전세계적으로 이에 대한 연구가 활발히 진행되고 있다. 이산화탄소를 직접 분자구조내에 삽입하는 기술 중 고분자 원료물질로 이용되고 있는 Dimethyl carbonate와 Polyol에 대한 제조기술 현황에 대해 소개하였다. RIST에서는 Dimethyl carbonate 제조기술로 urea methanolysis에 의한 촉매 및 공정을 개발하였으며, Polyol의 경우 고유 촉매개발 및 polyol 제품군에 대한 연구를 수행중에 있다. 이들은 분자내에 이산화탄소를 40%이상 포함할 수 있는 화학제품이므로 실용화 성공 시 온실가스 저감에 큰 기여를 할 수 있을 것으로 기대된다.

천연제올라이트를 이용한 메탄 하이드레이트 생성에 대한 연구 (A Study on the Methane Hydrate Formation Using Natural Zeolite)

  • 박성식;안웅진;김대진;전용한;김남진
    • 설비공학논문집
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    • 제23권4호
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    • pp.259-264
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    • 2011
  • Gas hydrate is formed by physical binding between water molecule and gas such as methane, ethane, propane, or carbon dioxide, etc., which is captured in the cavities of water molecule under the specific temperature and pressure. $1\;m^3$ hydrate of pure methane can be decomposed to the methane gas of $172\;m^3$ and water of $0.8\;m^3$ at standard condition. If this characteristic of hydrate is reversely utilized, natural gas is fixed into water in the form of hydrate solid. Therefore, the hydrate is considered to be a great way to transport and store of natural gas in large quantity. Especially the transportation cost is known to be 18~25% less than the liquefied transportation. However, when methane gas hydrate is artificially formed, its reaction time may be too long and the gas consumption in water becomes relatively low, because the reaction rate between water and gas is low. Therefore, for the practical purpose in the application, the present investigation focuses on the rapid production of hydrates and the increment of the amount of captured gas by adding zeolite into pure water. The results show that when the zeolite of 0.01 wt% was added to distilled water, the amount of captured gas during the formation of methane hydrate was about 4.5 times higher than that in distilled water, and the methane hydrate formation time decreased at the same subcooling temperature.

임업경쟁력 강화를 위한 유실수의 지역특화 연구 (A Study on Regional Specialization of Fruit Trees To Strengthen Competitiveness)

  • 김세빈;고영웅;오도교;노희경
    • 한국산림과학회지
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    • 제102권2호
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    • pp.292-299
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    • 2013
  • 본 연구의 목적은 단기소득임산물별 지역특화현황을 분석함으로써 임업경쟁력의 강화를 위한 단기소득임산물 활성화 방향을 제시하는 것이다. 분석결과, 밤은 공주시와 인접한 부여군, 청양군이 인접해있고 이들은 특화심화지역으로 분류되었다. 호두는 경북 김천시, 충북 영동군, 전북 무주군이 호두 재배면적 특화심화지역으로 서로 인접해 있다. 그 외 다른 특화심화지역들은 산발적으로 분포되어 있고, 이 지역들의 생산규모는 미약한 수준을 보이고 있다. 대추는 경산시를 중심으로 특화심화지역인 군위군, 청도군, 영천시가 서로 인접해 있으며, 이들 지역들의 대추생산량은 증가하는 추세이다. 떫은감은 상주시와 영동군이 인접해 있는 것을 제외하고 집단화되어 있지 않고 특화심화지역은 산발적으로 분포하고 있다.

17세기말기-18세기말 프랑스 남자 쥐스또꼬르 유형변화와 패턴제작 연구 (A Study of the Changes in Types of Justaucorps for French Men, and Pattern making from the End of the 17th Century to the End of the 18th Century)

  • 김양희
    • 복식
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    • 제59권8호
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    • pp.49-65
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    • 2009
  • As basic materials in this research, 874 pieces of engraving that recorded the garment produced by diverse authors in the 17-18th century were collected among the collections of male and female garment in the engraving kept by National Library of France and 216 pieces of engraving were classified among them to observe justaucorps as stated in the materials. Since type of justaucorps in the 18th century can be classified by difference in shape and change through the above engraving materials in large quantity and the shape and composition can be compared and analyzed with other materials such as picture, stored garment, written materials without difficulty, the chronology can be composed by single type of justaucorps. Intending to understand the change in composition caused by type classification and pattern making as per analysis of type property of justaucorps, the men's upper garment in France in the 18th century. Checking change in design per from and materials, justaucorps of 1680-1700s was in silhouette where waist in the length above knee was adhered and slightly spread to lower part. In the type of 1710-1750s, waist adhered remarkably and many pleats were made under waist to produce volume of form that spread to lower part like skirt. In the type of 1760-1780s, straight silhouette was attached to whole body and end of front adjustment in slant line turns to rear part. The developmental aspect of construction can be checked by making pattern. Changing the cutting line of body plate to smoothly connect section and to try to adhere to the body, the development of simpler and delicate pattern production technique was confirmed.

Novel technique for preventing make up deterioration using performance materials

  • Miyazawa, Masakazu;Nishikata, Kazuhiro;Mohri, Kunihiko
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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    • pp.370-378
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    • 2003
  • MFD (Make up Film Deterioration) is a gradual deterioration of applied make up and is a common problem experienced by most foundation users. Our investigation revealed that for 64% of all make up users MFD is their greatest consern is using foundations. Known that the primary cause of MFD is sebum secretion. We observed that the length of time prior to onset of MFD in people who produce high level of sebum varies significantly from person to person. This suggests that other factors besides quantity of sebum production can affect MFD. Control over this factor would, we believe, be key to developing longer-lasting makeups. We studied the relationship between MFD and skin surface conditions. Our study revealed that furrows on the skin surface affect MFD significantly. Sebum reaches the skin surface from sebaceous glands and flows along furrow on the skin. If there are many deep furrows, it takes longer for sebum to overflow. But if the furrows are few or shallow, sebum quickly overflows and spreads over the skin surface where it can degrade the make up film. Therefore even when the volume of sebum produced is the same, the rate of MFD will be different depending on the number and shape of the furrows. A longer-lasting foundation could be produced by matching personal skin condition, but this would be very difficult because individual variations in texture are very large. Therefore we approached the problem by attempting to impose sebum resistance in under make up and foundation. We have developed two new materials and make up products based on our theory. A new fluoroalkyl acrylate-methacrylates copolymer designed for incorporation in under make up is extremely sebum resistant and sweat proof. Another new acrylate polymer designed for inclusion in foundation absorbs sebum and changes to a solid. Usage tests confirm it is possible to reduce MFD by using under make up and foundation which incorporate our new materials to cover where skin furrows are few or shallow

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식이 Vitamin E가 Streptozotocin 유발 당뇨쥐 신장조직에서의 병리조직학적 변화에 미치는 영향 (Effects of Vitamin E on the Microstructural Changes of Renal Tissue in Streptozotocin-Induced Diabetic Rats)

  • 이순재;곽오계;임정교
    • 한국식품영양과학회지
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    • 제28권3호
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    • pp.663-669
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    • 1999
  • The purpose of this study was to investigate the effects of vitamin E on the histochemical change of kidney tissue in diabetic rats. Sprague Dawley male rats weighing 100$\pm$10g were randomly assigned to one normal and three STZ induced diabetic groups, which were subdivided into vitamin E free diet(DM 0E group), 40mg vitamin E per kg diet(DM 40E group) and 400mg vitamin E per kg diet(DM 400E group). Vitamin E level of normal group was 40mg per kg diet. Diabetes was exper imentally induced by intravenous injection of 55mg/kg of body weight of streptozotocin(STZ) in citrate buffer(pH 4.3) after 4 weeks feeding of experimental diets. Animals were sacrificed at the 6th day of diabetic states. The contents of thiobarbituric acid(TBARS) in kidney were increased 119%, 84% and 33% in DM 0E, DM 40E and DM 400E groups, respectively, compared to normal group. That of DM 400E group was decreased 39% compared to DM 0E group. Content of 2 microglobulin in urine in DM 0E, DM 40E, and DM 400E groups were increased by 248%, 181%, and 164%, respectively, compared to normal group. The diabetic groups showed the regressive lesion such as renal tubule, intumescence of epithelial cell, vacuolization. The results of the observation through electronic microscope showed the mitochondria shape of proximal tubule epithelial cell, irregular array, increase of ribosome, and irregular arrangement of small villosity, etc. These types of changes appeared severer in DM 0E group than in DM 400E group. These results indicate that the TBARS productions on kdney in STZ induced diabetic rats were increased, consequently those leaded to damage of renal tubule and minuteness structure. But a large quantity vitimin E supplementation was suppressed in TBARS production and improved in peroxidative damage of renal tissue so that relieved degenerative changes of renal tubule epithelial cell.

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KOH/KL제올라이트 및 Ca/미역촤를 이용한 대두유의 전이에스테르화 반응 (Transesterification of Soybean Oil Using KOH/KL Zeolite and Ca/Undaria pinnatifida Char)

  • 조용범;박성훈;전종기;박영권
    • 공업화학
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    • 제23권6호
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    • pp.604-607
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    • 2012
  • 본 연구에서는 표면적이 높은 제올라이트나 촤를 지지체로 이용해 알칼리금속을 담지시킴으로써 염기 세기를 증가시켜 전이에스테르화 반응에 있어 보다 좋은 활성을 가지는 고체촉매를 만들고자 하였다. 제올라이트는 KOH 수용액으로 담지하였고, 미역촤는 calcium nitrate를 담지하여 염기의 세기를 증가시켰다. Hammett 지시약과 $CO_2$-TPD를 통하여 촉매의 특성을 분석하였다. 대두유과 메탄올을 사용하여 바이오디젤을 합성한 후 지방산 메틸에스테르 함유량을 측정함으로서 촉매의 활성을 알아보았다. 일정량까지는 담지량과 활성이 비례하였으나, 과량 담지 시 오히려 활성이 감소하는 결과를 보였다.