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Non-invasive evaluation of embryo quality for the selection of transferable embryos in human in vitro fertilization-embryo transfer

  • Jihyun Kim;Jaewang Lee;Jin Hyun Jun
    • Clinical and Experimental Reproductive Medicine
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    • v.49 no.4
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    • pp.225-238
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    • 2022
  • The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth, ideally from the selection and transfer of a single competent embryo. Recently, techniques for efficiently evaluating the state and quality of preimplantation embryos using time-lapse imaging systems have been applied. Artificial intelligence programs based on deep learning technology and big data analysis of time-lapse monitoring system during in vitro culture of preimplantation embryos have also been rapidly developed. In addition, several molecular markers of the secretome have been successfully analyzed in spent embryo culture media, which could easily be obtained during in vitro embryo culture. It is also possible to analyze small amounts of cell-free nucleic acids, mitochondrial nucleic acids, miRNA, and long non-coding RNA derived from embryos using real-time polymerase chain reaction (PCR) or digital PCR, as well as next-generation sequencing. Various efforts are being made to use non-invasive evaluation of embryo quality (NiEEQ) to select the embryo with the best developmental competence. However, each NiEEQ method has some limitations that should be evaluated case by case. Therefore, an integrated analysis strategy fusing several NiEEQ methods should be urgently developed and confirmed by proper clinical trials.

A Study on the Speeding Intention and Behaviors Based on a Driver Behavior Questionnaire (DBQ를 이용한 운전자의 과속의도와 행동에 관한 연구)

  • Lee, Chang Hee;Kum, Ki Jung
    • Journal of Korean Society of Transportation
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    • v.33 no.2
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    • pp.159-169
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    • 2015
  • Speeding has been the most common traffic violation which increases the risk of accidents. The purpose of this study is to examine drivers' behaviors on the speeding intention and speeding action and to identify the relationship between those causes and effects. Effects of behaviors and human characters of drivers on speeding are analyzed through a Driver Behavior Questionnaire and the cause and effect among behavior characters, speeding intention and speeding behavior are validated through the structural equation model. In order to validate the hypothesis of the study, a path analysis is conducted through structural equation model. As the result, Driver Behavior Questionnaire property that influences the speeding is revealed to be the violation while Driver Behavior Questionnaire properties that influences the speeding behavior are lapse, mistake, and violation. And the speeding intention influences the speeding behavior. The study results are compared with previous studies to reveal that Driver Behavior Questionnaire properties influencing the speeding behavior are in the order of violation, mistake and lapse. Three properties of Driver Behavior Questionnaire, lapse, mistake and violation, are behavior scales in agreement with previous studies. The results of this study based on a Driver Behavior Questionnaire are expected to be utilized as a way to predict and validate driving behaviors.

Time-lapse Inversion of 3D Resistivity Monitoring Data (3차원 전기비저항 모니터링 자료의 시간경과 역산)

  • Kim, Yeon-Jung;Cho, In-Ky;Yong, Hwan-Ho;Song, Sung-Ho
    • Geophysics and Geophysical Exploration
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    • v.16 no.4
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    • pp.217-224
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    • 2013
  • We developed a time-lapse inversion using new cross-model constraints based on change ratio and resolution of model parameters. The cross-model constraint based on change ratio imposes the same penalty on the model parameters with equal change ratio. This constraint can emphasize the model parameters with significant change regardless of their increase or decrease. The resolution cross-model constraint imposes a small penalty on the model parameters with poor resolution, but a large penalty on the model parameters with good resolution. Thus, the model parameter with poor resolution can be effectively identified in the inversion result if they are significantly changed with time. Through the numerical tests for 3D resistivity monitoring data sets, the performance of these two cross-model constraints was confirmed. Finally, for the safety estimation of a sea dyke, we applied the developed time-lapse inversion to the 3D resistivity monitoring data that were acquired at a sea dike located in western coastal area of Korea. The result of time-lapse inversion suggested that there were no significant changes at the sea dike during the monitoring period.

Combined Treatment of Livestock Wastewater with Sewage Using Phanerochaete chrysosporium PSBL-1 (Phanerochaete chrysosporium PSBL-1을 이용한 축산폐수와 하수의 연계처리)

  • Lee, Soon-Young;Cho, Hong-Sik;Won, Chan-Hee
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.3
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    • pp.286-291
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    • 2006
  • We studied possibility of mixing treatment of livestock wastewater and sewage using Phanerochaete chrysosporium PSBL-1. Our study showed that 97.6% of SS and 95% of T-P removal efficiency was achieved when 2 mL BF02(a coagulant) and 100 mL C-210EL(a cationic polymer) were added to the mixture(2:1, v/v) of livestock wastewater and sewage. We studied treatment characteristic of Phanerochaete chrysosporium PSBL-1, after were mixed pretreated wastewater and sewage by dillution ten times about livestock wastewater. The removal efficiency of NBDCOD(non-biodegradable COD), $NH_3-N$ and T-N was increased according to increase of pH. That is, T-N concentration of effluent was satisfied 60 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities with 35 mg/L from a lapse of five days at pH 6.7, 51 mg/L from a lapse of three days at pH 8 and 33 mg/L from a lapse of one day at pH 10. Moreover $COD_{Mn}$ concentration of effluent was satisfied 40 mg/L by drain water waterqulity standard of livestock wastewater public treatment facilities after a laps of one day at all pH. Organics and nitrogen concentrations of effluent were higher case with addition of V.A.(veratryl alcohol) than case without addition of V.A.(veratryl alcohol). $COD_{Mn}$ concentration of effluent satisfied drain water qulity standard of livestock wastewater public treatment facilities from a lapse of one day, when C/N rate(3:1) of influent was not controled. T-N satisfied that from a lapse of two days, when C/N rate was controled with $4{\sim}6$.

Identification and Characterization of Microbial Community in the Coelomic Fluid of Earthworm (Aporrectodea molleri)

  • Yakkou, Lamia;Houida, Sofia;Dominguez, Jorge;Raouane, Mohammed;Amghar, Souad;Harti, Abdellatif El
    • Microbiology and Biotechnology Letters
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    • v.49 no.3
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    • pp.391-402
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    • 2021
  • Earthworms play an important role in soil fertilization, interacting continually with microorganisms. This study aims to demonstrate the existence of beneficial microorganisms living in the earthworm's immune system, the coelomic fluid. To achieve this goal, a molecular identification technique was performed, using cytochrome c oxidase I (COI) barcoding to identify abundant endogenic earthworms inhabiting the temperate zone of Rabat, Morocco. Then, 16S rDNA and ITS sequencing techniques were adopted for bacteria and fungi, respectively. Biochemical analysis, showed the ability of bacteria to produce characteristic enzymes and utilize substrates. Qualitative screening of plant growth-promoting traits, including nitrogen fixation, phosphate and potassium solubilization, and indole acetic acid (IAA) production, was also performed. The result of mitochondrial COI barcoding allowed the identification of the earthworm species Aporrectodea molleri. Phenotypic and genotypic studies of the sixteen isolated bacteria and the two isolated fungi showed that they belong to the Pseudomonas, Aeromonas, Bacillus, Buttiauxella, Enterobacter, Pantoea, and Raoultella, and the Penicillium genera, respectively. Most of the isolated bacteria in the coelomic fluid showed the ability to produce β-glucosidase, β-glucosaminidase, Glutamyl-β-naphthylamidase, and aminopeptidase enzymes, utilizing substrates like aliphatic thiol, sorbitol, and fatty acid ester. Furthermore, three bacteria were able to fix nitrogen, solubilize phosphate and potassium, and produce IAA. This initial study demonstrated that despite the immune property of earthworms' coelomic fluid, it harbors beneficial microorganisms. Thus, the presence of resistant microorganisms in the earthworm's immune system highlights a possible selection process at the coelomic fluid level.

A Study on the Correlation between the Lapse of Time and Diagnostic Test Results of Large Rotating Machine Windings (대형 회전기의 절연진단결과와 경년 특성과의 상관관계에 관한 연구)

  • Kim, K.H.;Cho, Y.O.;Sun, J.H.;Kim, Y.B.;Ryu, H.S.
    • Proceedings of the KIEE Conference
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    • 1992.07b
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    • pp.966-968
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    • 1992
  • We have done the insulation diagnostic test of generators in domestic five power plants since 1987 and tested A.C. current increasing rate($\Delta$I), $\Delta$tan$\delta$ and partial discharge in generator winding insulation. This study is described the correlation between nondestructive result(A.C. current increasing rate, $\Delta$tan$\delta$ and partial discharge) and lapse of time in generators.

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Reproduction of Rhodochorton purpureum from Jeju Island, Korea and SanJuan Island, Washington, USA in Laboratory Culture

  • West, Kathryn A.;West, John A.;Lee, Yong-Pil
    • ALGAE
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    • v.21 no.1
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    • pp.103-107
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    • 2006
  • Rhodochorton purpureum 4187 from Jeju Island, Korea may have a sexual life history similar to that seen by other investigators working on other strains around the world. In culture short days (8:16, 11:13, 12:12 LD) at 10-15°C induced tetrasporogenesis. Discharged spores were observed with time lapse videomicroscopy. They showed a slight amoeboid movement for 2-3 minutes before rounding up and settling. Tetrasporelings develop into male and female gametophytes. No fertilisation was observed. Tetrasporangia often were borne on carpogonial clusters of females but no discharged spores were seen. Isolate 4241 from San Juan I., Washington, USA grew well in most conditions tested but did not reproduce in short days (8:16, 11:13, 12:12 LD) at 10-15°C.

Borehole radar monitoring of infiltration processes in a vadose zone

  • Jang, Han-Nu-Ree;Park, Mi-Kyung;Kuroda, Seiichiro;Kim, Hee-Joon
    • 한국지구물리탐사학회:학술대회논문집
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    • 2007.06a
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    • pp.313-316
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    • 2007
  • Ground-penetrating radar (GPR) is an effectiveness tool for imaging spatial distribution of hydrogeologic parameters. An artificial groundwater recharge test has been conducted in Nagaoka City in Japan, and time-lapse crosshole GPR data were collected to monitor infiltration processes in a vadose zone. Since radiowave velocities in a vadose zone are largely controlled by variations in water content, the increase in traveltimes is interpreted as an increase in saturation in the test zone. We use a finite-difference time-domain method in two-dimensional cylindrical coordinates to simulate field results. Numerical modeling successfully reproduces the major feature of velocity changes in the filtration process.

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A study on the shapes of thermal corrosion in sandwich panel (샌드위치 패널의 열적 손상에 의한 부식 형상에 관한 연구)

  • Kim, Hyeon-Dong
    • Journal of Korean Institute of Fire Investigation
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    • v.3 no.1
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    • pp.43-46
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    • 2003
  • In this experimental report, We introduce an idea of thermal corrosion in sandwich panel. In brief, Because the structures of sandwich panel are easily collapsed by thermal damage caused by a fire, it is very difficult to investigate a point and origin of fire. Therefore, If the shapes of thermal corrosion are reconstructed according to change of temperature and lapse time by experiment in sandwich panel, it is very simple task that fire scene investigators search for a point and origin of fire. As a result, we present the difference of thermal corrosion between samples which are applied heat by many-sided variable, such as temperature, heating time, lapse time, humidity, and others.

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