• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.210.2-210.2
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• 2003

• Applied Biological Chemistry
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• 제6권
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• pp.119-121
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• 1965

• ALGAE
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• 제19권1호
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• pp.31-37
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• 2004

Farmed kelp gametophytes were previously observed to be living endophytically in filamentous red algae. The interactions of two farmed kelp species and six red algae were examined in laboratory culture. Undaria pinnatifida (Harvey) Suringar and Laminaria religiosa Miyabe demonstrated the differing abilities of zoospores to become endophytic in four host red algae and neither kelp became endophytic in two non-filamentous red algae. There was a strong seasonal component regarding infectiousness that is associated with the changes in fron erosion in U. pinnatifida from April to June. At the same time, L. religiosa showed no significant changes in frond erosion, and there were no apparent changes in infection levels in the two species they were able infect. This study indicated clear differences between two keip species with regard to their symbiotic relationship to red algae in terms of host specificity and preference of kelp gametophytes.

• 대한의생명과학회지
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• 제19권3호
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• pp.206-212
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• 2013

The herbal composition Gyeongshingangjeehwan 18 (GGEx18), which is composed of three herbs, Laminaria japonica Aresch (Laminariaceae), Rheum palmatum L. (Polygonaceae), and Ephedra sinica Stapf (Ephedraceae), has been used as an anti-obesity drug in Korean local clinics. Thus, we investigated whether GGEx18 regulates obesity by suppressing appetite in high fat diet-induced obese C57BL/6J mice. Administration of GGEx18 to obese mice for 9 weeks significantly decreased body weight gain, epididymal adipose tissue weight, and food efficiency ratio. GGEx18 also caused a significant decrease in the circulating levels of leptin, which were increased by about 450% in obese control mice compared with normal lean mice. Concomitantly, GGEx18 decreased mRNA levels of a potent appetite-stimulating hormone neuropeptide Y, but increased an appetite-suppressing hormone pro-opiomelanocortin mRNA levels. These results suggest that GGEx18 may prevent obesity through regulating appetite in nutritionally obese mice.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.208-211
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• 2003

Characteristics of biosorption of lead by marine algae, Laminaria japonica, were examined. The biosorption capacity of lead by L. japonica was achieved up to 30％ of its own weight and proportional to the initial lead concentration. However, the opposite result was shown in different initial weight of biomass. Ion exchange reaction between lead ions and calcium ions was observed on lead biosorption with Ca-Ioaded biomass. Stoichiometric coefficient, which can represent the exchange ratio between metal ions and protons during elution process, was determined as 1.39. Therefore, it was concluded that the reaction between lead ions already attached in biomass and protons in bulk solution was not fully stoichiometric ion exchange relation at elution process.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 추계학술대회 및 정기총회
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• pp.127-127
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• 2003

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2007년도 춘계 학술발표회 발표논문집
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• pp.275-281
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• 2007

• 한국양식학회:학술대회논문집
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• 한국양식학회 2005년도 춘계학술발표대회 논문요약집
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• pp.48-48
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• 2005

• ALGAE
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• 제20권2호
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• pp.151-155
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• 2005

To obtain basic data, we investigated the effect of blade length on transplants, the transplanting method of Laminaria japonica for creating L. japonica resources and the number of transplanting plates with surviving L. japonica. The survival rate of L. japonica, blade length of transplants and drag force of transplanting plates were also researched. The number of transplanting plates with surviving L. japonica, the survival rate and blade length of 20 cm long-initial transplants were greater than those of 1.5, 5 and 10 cm long-initial transplants in an outdoor aquarium. At the depth of 4 m in the coastal waters, the number of transplanting plates with surviving transplants, the survival rate and the blade length of 30 cm long-initial transplants were higher than those of 10 and 20 cm longinitial transplants. The drag force is calculated by cording up sporophytes of L. japonica into the transplanting plates under water. The drag force in the case of a 2.18 kg-weight transplanting plate and in a current speed of 0.5 m${\cdot}s^{-1}$ for considering stability of the plate was 631.50 g to a concrete substratum on the seabed, 703.92 g to a shingle substratum, 788.00 g to a sand substratum, and 1018.30 g to a silt substratum. If we consider the stability and economic efficiency of the transplanting plate, the proper weight of the plate per one individual of 18.11 cm in blade width and 190.20 cm in total blade length is regarded as 508.2 g when it is calculated with the concrete substratum that shows the lowest drag force.

• 한국양식학회지
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• 제19권1호
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• pp.33-39
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• 2006

Anticoagulant activities of a fermented edible brown alga, Laminaria ochotensis was investigated. L. ochotensis was fermented with 15% sugar (w/v) at $25^{\circ}C$ for 10 weeks. Anticoagulant activity was measured from the supernatant of algal mixture at biweekly intervals up to $10^{th}$ week by activated partial thromboplastin (APTT), prothrombin time (PT) and thrombin time (TT) assay using citrated human plasma. Sample having high APTT activity $(6^{th}\;week)$ was filtered, ethanol precipitated and freeze-dried. The polysaccharide compound having anticoagulant activity was purified by DEAE ion exchange chromatography followed by Sepharose-4B gel filtration chromatography. Anticoagulant activity, polysaccharide concentration, and heparin like activity were determined for the collected fractions by APTT, $phenol-H_2SO_4$, and glycosaminoglycan assay, respectively. The anticoagulant activity assay showed that the activity was increased up to $6^{th}$ week, and decreased thereafter. The concentration of our purified compound was $31.0{\mu}g/ml$ and showed higher APTT activity than commercial heparin. At the same concentration of $31.0{\mu}g/ml$, the heparin showed 186.5 sec activity while our purified compound showed an activity of 386 sec. Single spot on agarose gel electrophoresis showed that the compound was purified and polyacrylamide gel electrophoresis (PAGE) results revealed that the molecular mass of the purified polysaccharide compound was between 60 and 500 kDa. Therapeutic interest of the algal polysaccharide as an anticoagulant has recently been in highlighted. This purified anticoagulant compound from fermented L. ochotensis can be used as a model for anticoagulant agent or could be developed as an anticoagulant agent. This study can be extended to identify the structure and chemical composition of the purified polysaccharide, and to establish a relationship between structure and the function of the identified anticoagulant compounds.