• 대한수의학회지
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• 제38권3호
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• pp.664-673
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• 1998

초음파학적 검사에 따라 미만성 저에코 영역, 낭포, 다병소성 낭포 및 비후된 피막 등 비정상적 소견을 보이는 전립선에서 추출한 전립선액과 생검조직에서의 LDH 분획비(LDH I/V)의 평균치는 각각 $0.92{\pm}0.55$, $2.69{\pm}0.82$, 정상군(각각 $0.23{\pm}0.20$, $0.57{\pm}0.36$)에 비하여 유의성(p<0.01) 있게 높았다. 세포병리학적 검사에서 전립선 비대증 및 전립선염으로 나타난 비정상군($2.76{\pm}0.77$의 생검조직 LDH 평균분획비는 정상군($1.38{\pm}1.19$)에 비해 유의성 있게 높았으나 전립선액에서는 분획비의 유의차가 인정되지 않았다. 배양결과에 따른 전립선액 및 조직시료의 LDH 분획비는 정상군과 비정상군 사이에서의 유의성은 인정되지 않았다. 결론적으로 저에코 영역 및 낭포부위는 생검조직과 전립선액의 배양 및 세포병리학적 검사에 따른 비정상성 및 높은 LDH 분획비와 밀접한 연관성을 보였다. 특히 경직장 초음파상에 나타나는 미만성 저에코 영역은 전립선 비대증 및 만성 전립선염 등과 같은 병적 관련성을 내포하고 있는 것으로 사료된다.

• 대한수의학회지
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• 제20권2호
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• pp.127-134
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• 1980

This study was performed to obtain an accurate methods of diagnosis in early stage of traumatic pericarditis and myocarditis in ruminants. Korean native goats were used as experimental animal and traumatic pericarditis and myocarditis were induced by surgical intervention. The changes in clinical signs, hematological values, blood enzymes and electrocardigrams were observed for 14 days after operation. The results obtained were as follows: 1. Body temperature in experimental group of induced traumatic pericarditis and myocarditis was slightly increased than control group that was rumenotomized. Heart rate was increased in both groups but the increased rate was noticed remarkably in experimental group. There was no change in respiratory rate in experimental and control group. 2. Total leucocytes counts and neutrophil percentage maintained higher level after operation and lymphocytes percentage noticed reversed proportion to neutrophil in both groups. 3. Serum total protein concentration and alkaline phosphatase activities after operation showed a little lower levels than these values prior to operation. Serum glutamic oxaloacetic transaminase and lactic dehydrogenase activities in control group showed slight increase through all the experimental period, while in experimental group these activities were markedly elevated and an increase in serum lactic dehydrogenase activities was more remarkable comparing to serum glutamic oxaloacetic transaminase. 4. Electrocardiograms in control group did not show any abnormalities except for slight increase in heart rate. In experimental group the amplitude of QRS complex was increased in duration of experimental period. The transference from QRS complex to T wave was uncertain and S-T segment moved upward obliquely through electrical zero line.

• 대한한의학회지
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• 제19권1호
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• pp.251-270
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• 1998

The effects of Gamigunshimtang on the isolated perfused ischemic heart in rats, heart rates, left ventricular pressure, cardiac blood flow and cardiotoxicity were stu.died in H9C2 myoblast cell, myocardial slice culture The results were as follows: 1. The administration of Gamigunshimtang to the rat recovered effectively heart rate, left ventricular pressure and flow rate from the experimental ischemia in perfused rat heart. The release of lactic dehydrogenase after the ischemia also decreased compared to the control group. 2. The administration of Gamigunshimtang to H9C2 myoblast culture enhanced the cell proliferation and protected against doxorubicin and allylamine induced release of the lactic dehydrogenase into the culture medium. It also protected effectively against doxorubicin and allylamine induced decrease of Ca ATPase activity and the increase of NADPH-cytochrome C reductase activity in the microsome. 3. The administration of Gamigunshimtang to the rat myocardial slice culture protected effectively against doxorubicin and allylamine induced decreases of protein synthesis and ATP content, and increases of cvtosolic enzyme, creatin kinase into the medium and lipid peroxidation.

• 한국식품저장유통학회지
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• 제18권5호
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• pp.795-802
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• 2011

Carbonic maceration처리 포도주에 있어 유기산 특히 사과산 함량을 감소시키는 주요 원인을 찾고자 포도를 2주 동안 온도별 carbonic maceration 처리하며 시기별로 산함량 및 사과산대사 관련 효소활성을 측정하였다. 온도별 carbonic maceration 처리 포도의 pH는 CM-$25^{\circ}C$와 CM-$35^{\circ}C$에서 처리 시간이 경과할수록 가장 높았으며, 총산은 초기에는 CM-$35^{\circ}C$에서 가장 낮은 함량을 나타내었지만 처리 6일 이후 서서히 증가하였고, CM-$25^{\circ}C$는 처리 완료일 까지 꾸준히 감소하는 것으로 나타났다. CM-$45^{\circ}C$는 초기와 비슷한 함량을 나타내어 다른 처리보다 높은 총산 함량을 나타내었다. 유기산 함량에 있어 사과산 함량은 CM-$35^{\circ}C$에서 가장 많이 감소하였고, 젖산 함량은 CM-$35^{\circ}C$에서 가장 높게 나타났다. 사과산 대사과련 효소활성을 살펴본 결과, malic enzyme과 malic dehydrogenase는 CM-$25^{\circ}C$와 CM-$35^{\circ}C$에서 가장 높은 효소활성을 나타내었지만 CM-$45^{\circ}C$에서는 초기부터 효소활성이 나타나지 않았다. oxalacetate decarboxylase 활성도 malic dehydrogenase 활성과 비슷한 경향을 나타내었다. pyruvate decarboxylase 활성은 다른 효소활성에 비해 활성이 낮았지만, CM-$45^{\circ}C$에서도 활성을 나타내었다. 반면 L-lactic dehydrogenase 활성은 어떤 처리구에서도 나타나지 않았다. 이와 같은 결과로부터 온도와 효소활성과의 관계에 있어 온도가 $40^{\circ}C$ 이상에서는 사과산 대사관련 효소가 활성을 나타내지 않는 것을 알 수 있었고, carbonic maceration 처리에서 사과산 감소가 효소의 작용에 크게 영향 받는 것을 확인할 수 있었지만, 젖산 생성에 대해서는 효소작용 외에 사과산 대사 미생물과 같은 다른 요인들에 대해 좀 더 깊이 있는 연구가 필요하다.

• 대한암한의학회지
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• 제20권1호
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• pp.81-88
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• 2015

Generally, normal cells synthesize adenosine triphosphate (ATP) through oxidative phosphorylation in the mitochondria. However, they produce ATP through lactic acid fermentation on hypoxic condition. Interestingly, many cancer cells rely on aerobic glycolysis for ATP generation instead of mitochondrial oxidative phosphorylation, which is termed as "Warburg effect". According to results from recent researches on differences of cancer cell metabolism from normal cell metabolism and because chemotherapy to suppress rapidly growing cells, as a side effect of cancer treatment, can still target healthy cells, there is merit in the development of small-molecule inhibitors targeting metabolic enzymes such as pyruvate dehydrogenase kinase (PDHK), lactate dehydrogenase (LDH) and monocarboxylate transporter (MCT). For new anticancer therapy, in this review, we show recent advances in study on cancer cell metabolism and molecules targeting metabolic enzymes which are importantly associated with cancer metabolism for cancer therapy. Furthermore, we would also like to emphasize the necessity of development of molecules targeting metabolic enzymes using herbal medicines and their constituents for anticancer drugs.

• 대한의생명과학회지
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• 제12권3호
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• pp.233-240
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• 2006

We investigated enzyme activity and histochemical changes in hind limb of mouse treated with 6-aminonicotinamide (6-AN). The activity of aspartate aminotransferase, alanine aminotransferase and creatine phosphokinase in 6-AN treated group were significantly higher than those of the control and pair-fed groups. Also, the activity of lactic dehydrogenase in 6-AN treated group was the highest among the three groups, whereas that of the pair-fed group were higher than that of the control group. In the 6-AN treated group, oxidative histochemical stains, nicotinamide adenine dinucleotide reductase (NADH), succinyl dehydrogenase (SDH) showed increased scattered fibers in 6-AN treated subsarcolemma. Cytochrome c oxidase (COX) stain showed decreased up to 85% in 6-AN treated fibers. These results demonstrate that 6-AN antagonizes cell metabolism and induces the morphological deformity like the other mitochondrial muscle diseases. Therefore, we suppose that these data would be useful indexes for disclosing the mechanism of mitochondrial muscle disease.

• 생명과학회지
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• 제28권10호
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• pp.1193-1200
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• 2018

본 연구에서는 전통적인 방법으로 제조한 천도복숭아식초의 이화학적 특성과 항산화 작용, 아질산염 소거능 및 알코올 대사 효소 활성을 알아보았다. 천도복숭아식초의 pH는 3.70이였고, 당도는 $8.87^{\circ}Brix$였으며, 총산도는 6.29%로 나타났다. 천도복숭아식초의 유기산 분석 결과 acetic acid가 32.42 mg/ml로 가장 높았고, 그 다음으로 lactic acid, succinic acid, malic acid의 순으로 나타났다. 천도복숭아식초의 총 페놀함량은 $121.84{\mu}g\;TAE/100ml$로 나타났다. 천도복숭아식초의 DPPH radical 소거능은 농도 의존적으로 증가하였으며 40% 농도에서 84.47%의 소거능을 보였다. SOD 활성 또한 농도 의존적으로 증가하였으며 60% 농도에서 89.06%로 나타났다. 아질산염 소거능 분석에서는 천도복숭아식초 100% 농도, pH 1.2, 3.0, 6.0에서 각각 94.17, 76.91, 20.21%로 pH가 낮을수록 아질산염 소거능이 증가하는 것으로 나타났다. 천도복숭아 식초의 알코올 분해효과를 알아보기 위하여 ADH 활성 및 ALDH 활성을 분석한 결과, ADH 활성 및 ALDH 활성 모두 농도 의존적으로 증가하였으며, 60% 농도에서 각각 153.61%와 178.20%로 나타났다. 이상의 결과에서와 같이 전통적인 방법으로 제조한 천도복숭아식초는 기능성 건강음료로서의 가능성이 있음을 확인할 수 있었다.

• 생명과학회지
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• 제32권4호
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• pp.290-297
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• 2022

본 연구에서는 전통적인 방법으로 제조한 황칠 식초의 유기산 함량을 측정하고 항산화 효과와 항당뇨 효과, 알코올 대사 효소 활성 및 HepG2 세포에 대한 간보호 효과를 알아보았다. 황칠 식초의 유기산 분석결과 acetic acid가 91.72 mg/ml로 가장 높았고, 그 다음으로는 lactic acid (7.31 mg/ml), malic acid (1.36 mg/ml), succinic acid (1.20 mg/ml) 순으로 나타났다. 황칠 식초의 총 폴리페놀 함량은 13.73 ㎍ TAE/ml으로 나타났다. DPPH radical 소거능은 농도 의존적으로 증가하였으며 60% 농도에서 76.04%로 나타났고, SOD 활성은 60% 농도에서 95.14%로 나타났다. 황칠 식초 10% 농도에서의 α-glucosidase 저해 효과는 98.94%로 높게 나타났다. 알코올 분해 활성을 알아보기 위해 ADH 및 ALDH 활성을 측정한 결과, 두 가지 효소 모두 황칠 식초의 농도 의존적으로 증가하였으며 60% 농도에서의 ADH 및 ALDH 활성은 각각 43.62% 및 60.39%로 나타났다. 황칠 식초 0.6% 농도에서는 tacrine으로 유도된 HepG2 세포주에 대하여 간보호 활성이 높게 나타났다. 이상의 결과에서 전통적인 방법으로 제조한 황칠 식초는 기능성 건강 음료로서 활용 가능성이 높은 것으로 생각된다.

• 미생물학회지
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• 제15권3호
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• pp.103-112
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• 1977

Several strains of spore-forming lacticacid bacteria were isolated from natural sources such as soils, cereals, and foods. The general morphological and physiological characteristics of the strain 6-4 were investigated nad compared with some other industrial strains. The effects of fructose-1,6-diphoshpate (FDP), adenosine triphosphate (ATP), and pH on the lactate dehydrogenase(LDH) activity of the strain were studied, and the changes in LDH activity and spore formation under various cultural conditions were researched. The results were as follows. 1. This strain was identified to Bacillus coagulans Hammer and distributed widely in natural sources. 2. The strain strongly converted various fermentation substrates in to L(+)-lacticacid in anaerobic conditioins, and many spores that were of great advantages to the industrial application were formed easily in the aerobic condition. 3. The LDH activity of this strain was activated by FDP and inhibited by ATP. The optimal pH for the enzyme activity was 6.0-6.5. 4. In the anaerobic culture condifion, the large amount of glucose added in the medium increased the LDH activity, but the cells were not committed to sporulate. 5. When none or a very small amount of glucose (less than 0.5%) was added to culture medium in the aerobic condition, the LDH activity was decreased and many spore were produced with final pH higher than 8.5. 6. The additioin of large amount of glucose (more than 2.0%) in aerobic culture increased the LDH activity and inhibited strongly the spore formation with final pH lower than 6.0.

• 한국식생활문화학회지
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• 제27권6호
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• pp.743-750
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• 2012

A tissue cultured wild ginseng (TCWG) suspension was inoculated with lactic acid bacteria and fermented to improve the functionality of TCWG. The utilization of TCWG was increased directly using the freeze-dried powder. The optimal ratio of TCWG powder and water for fermentation was 1:19 (5%), which was selected by measuring the fluidity and viable cell count according to concentration. The effects on ADH activation and immune cell activation by each ferments with 10 kinds of Lactobacillus sp. strains were examined. The ferments with the Lactobacillus casei KFRI 692 strain showed 5.4 times higher ADH activity and 1.3 times higher ALDH activity than the non-fermented TCWG powder (control). The level of NO production and cytotoxicity was also measured by Raw 264.7 cells. The ferment with the Lac. casei KFRI 692 strain showed the highest level of NO production and lower cytotoxicity than the others. Therefore, the Lac. casei KFRI 692 strain was selected as a strain for fermentation of a TCWG suspension to maximize its functionality. To identify the optimal fermentation time of the selected Lac. casei KFRI 692 strain on the 5% TCWG suspension, the viable cell count of lactic acid bacterial and the changes in pH were observed for 72 hours. 24-hrs was found to be the optimal fermentation time. In this way, fermented TCWG with lactic acid bacteria showed higher ADH activation efficacy and immune cell activation than non-fermented TCWG.