• The Korean Journal of Mycology
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• v.16 no.4
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• pp.242-246
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• 1988

To determine contents of inorganic elements of Laccaria, the carpophores of Laccaria laccata(Fr.) Berk. et Br., Laccaria amethystina(Fr.) Berk. et Br., Laccaria tortilis(S.P. Gray) Cooke and Laccaria vinaceoavellanea Hongo were incinerated and analyzed by atomic absorption spectrophotometer. Total contents of determined inorganic elements(except Cd) were highst in Laccaria tortilis than the other three mushrooms, and Laccaria vinaceoavellanea, Laccaria laccata and Laccaria amethystina in that order. The four species mushrooms contained potassium, iron, zinc, sodium, copper, manganese, and calsium in that order, and the content of potassium was the highst than the other inorganic components. The content of cadmium was Laccaria tortilis, Laccaria laccata, Laccaria vinaceoavellanea and Laccaria amethystina in that order.

• Archives of Pharmacal Research
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• v.5 no.1
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• pp.17-19
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• 1982

To develop potent anitumor polysac-charides from higher hungi of Korea the alcohol precipitated polysaccharides, which were obtained from hot water extracts of shake-cultured mycelia of eight species of basidiomycetes, were subjected to antitumor screening tests, using sarcoma 180 as a tumor cell. When administered i. p, at the dose level of 20mg/kg/day for ten days into the mice which were implanted with $1{times}10^{8}$ cells of sarcoma 180 twenty-four hour before the start of polysaccharide injection, the polysaccharides of Plutcus cervinus ISA-Pc-1004, Pleurotus pulmonarius, Hydnum repandum ISA-Hr-1006, and Laccaria laccata ISA-LI-1008 respectively showed inhibition ratios of 62.0%, 51.7%, 64.8%, and 57.8%. They were selected for further study, while those of Pancillus serotina ISA-Ps-1012, Laccaria amethystina ISA-La 1001, Pholiota adiposa ISA-Pa-1010, and Lepista spISA-Ls-1007 respectively showed inhibition ratios of -9.3%, 25.3%, 27.3% and 33.0%.

• The Korean Journal of Mycology
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• v.12 no.1
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• pp.35-43
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• 1984

To produce and characterize antineoplastic constituents in the submerged cultured­mycelia of Laccaria laccata, the mycelia were extracted with distilled water. Purification of the extract was carried out by acetone precipitation, by ion exchange chromatography using DEAE­Sephadex A-50, CM-Sephadex C-25 resins, and by gel filtration chromatography on Sephadex G-200. Each fraction obtained during the purification was examined for antineoplastic activity against sarcoma 180 in ICR mice. As the purification proceeded, the antineoplastic activity was markedly increased. The highly purified Fraction E showed 75% tumor inhibition ratio at a dose of 10mg/kg/day and contained 81% polysaccharide and 4% protein. The antitumor component of Fraction E stimulated an accumulation of peritoneal exudate cells including peritoneal macrophages, and is named laccaran.

• The Korean Journal of Mycology
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• v.10 no.4
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• pp.155-163
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• 1982

To find antitumor components, a protein-bound polysaccharide fraction was obtained by extracting with hot water and precipitating with ethanol from the carpophores and the cultured mycelia of Laccaria laccata. The fraction was examined for antitumor activity against sarcoma 180 implanted in mice. The component extracted from the carpophores showed 75% and 65% tumor inhibition ratios in the doses of 20 mg and 50 mg/kg/day, respectively. The protein bound polysaccharide fraction of the cultured mycelia of L. laccata also showed 57.8% tumor inhibition ratio in the dose of 20 mg/kg/day. The chemical analysis of the protein-bound polysaccharide fraction showed that it contained a polysaccharide and a protein. The hydrolysates of the polysaccharide moiety contained six and one unknown monosaccharides. Fourteen amino acids were identified in the hydrolysates of the protein moiety.

• Journal of Mushroom
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• v.3 no.4
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• pp.145-153
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• 2005

Two hundred seventy-four microorganisms were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains and their microbial diversity was analyzed. The specimens of macrofungi were isolated from the soil of abandoned mines around for selecting the heavy metal-degrading strains. Eunseong and Dogok in Gyeongsangbuk-do, for selecting the heavy metal-degrading strains. Soil was also collected from same location. Contents of heavy metals (Cd, Cu, Pb, Zn, Ni, Cr, and As) were determined spectrometically in fruiting bodies of forty eight wild macrofungi specimens with soils. When the analysed their transfer factor from soil to fruiting body, Amanita volvata have the highest transfer factor of cadmium and arsenic. And Mycena pura showed the highest in mercury, Marasmius pulcherripes in zinc, Laccaria laccata in nickel, and Collybia confluens in chrome. When compare the population of mushrooms between the contaminated mines and Mt. Chiak as the healthy area, Genus Russula and Collybia were collected both area, but Leucocoprinus, Coprinus, Suillus, Lepiost, Gyroporus, Lepista, Microstoma, Stropharia, and Agrocybe were only in the contaminated mine area.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.429-444
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• 1998

Objective of this study was to select ectomycorrhizal fungi for black spruce(Picea mariana) inoculation to overcome the growth inhibitory effects of Kalmia angustifolia. Nineteen isolates representing 11 species of ectomycorrhizal fungi were tested for their abilities to grow and form mycorrhizae with black spruce seedlings in the presence of water leachate of leaves of Kalmia. Mycelium growth of 9 isolates were inhibited by the leaf leachate. Colony diameter and biomass of the other 10 isolates were either increased or unaffected under the same conditions. Acidic pH of the culture medium(pH 3 and 4) inhibited some of the fungi, but a combination of acidic pH and the leaf leachate was more inhibitory. Thirteen isolates were able to form ectomycorrhizae with black spruce in presence of 25% leaf leachate in pure culture. Four isolates, Paxillus involutus(NF4), Cenococcum geophilum(GB12), Laccaria laccata(GB23), and E-strain(GB45) formed mycorrhizae more successfully than the others in presence of up to 50% Kalmia leaf leachate. Black spruce seedlings pre-inoculated with these fungi were grown with Kalmia leaf leachate and live Kalmia plants during a four month greenhouse experiment. Abundant mycorrhizae(77-91% of root tips) were developed on seedlings pre-inoculated with P. involutus, L. laccata and E-strain but relatively poor mycorrhization(32% of root tips) resulted with C. geophidum. Over 90% of the short root mycorrhizae were attributed to the inoculated fungi although indigenous mycorrhizae also occurred on most seedlings. Persistence of the mycorrhizae was not affected by living Kalmia plants. Over 80% of the mycorrhizae on seedlings inoculated with P. involutus, L. laccata and E-strain and 53% of the mycorrhizae on seedlings inoculated with C. geophilum were attributable to the inoculant fungi. Control seedlings formed about 45% ectomycorrhizal short roots with indigenous fungi. The L. laccata and C. geophilum inoculated seedlings exhibited enhanced mycorrhizae formation in presence of Kalmia leaf leachate. Mycorrhizae formation with inoculant fungi was 4-15% lower at pH 4 than at pH 5, with the greatest inhibition occurring for L. laccata. Seedlings inoculated with P. involutus had the greatest shoot and root growth followed by L. laccata and E-strain inoculated seedlings. The P. involutus and L. laccata inoculated seedlings were significantly taller with more shoot dry biomass than the uninoculated(control) seedlings. E-strain inoculated seedlings had significantly higher shoot dry biomass and significantly lower number of first order lateral roots compared to the control but other growth parameters such as height, root dry weight and number of short root tips were not significantly different from the control. Seedlings inoculated with C. geophilum were not significantly different from the uninoculated seedlings in any of the growth parameters except for the number of first artier lateral roots which was significantly less than the control seedlings.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.48-69
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• 1987

To understand host ranges and host specificity of ectomycorrhizal fungi and fungus specificity of host tree species, higher fungi from pure forest stands of following twelve tree species were collected for six years from 1981 to 1986: Pinus densiflora, P. rigida, P. koraiensis, Picea koraiensis, Larix leptolepis, Abies holophylla, Populus $alba{\times}glandulosa$, Castanea crenata, Quercus aliena, Q. acutissima, Q. mongolica, and Betula platyphylla. A total of 196 ectomycorrhizal species with 8 varieties in 48 genera were identified. Thirty-one species with one variety belonged to Russula, 22 species with 5 varieties to Amanita, 18 species with one variety to Lactarius. Fungi belonging to above three gepera, Laccaria, and Cantharellus had a relatively wide host range. Laccaria laccata was collected under all the 12 tree species, Amanita vaginata group under 11 tree species, Laccaria amethystina and Russula foetens under 10 tree species, Lactarius gerardii and Russula sororia under 9 tree species, and Amanita agglutinata, Cantharellus cibarius, Russula bella, and R. virescens under 8 tree species. Above 10 fungal species may be classified to have a wide host range. Following fungi were collected under 4 to 5 tree species of both conifers and broad-leaved trees: Amanita citrina, Boletus bicolor, B. erythropus, Lactarius piperatus, L. subzonar­ius, and Russula pseudodelica. Above fungi may be classified to have an intermediate host range. Chroogomphus, Gomphidius, Rhizopogon, and Suillus were collected only under Pinaceae and may be classified to have a narrow host range. Particularly Suillus grevillei was collected only under Larix leptolepis, and appeared to have high host specificity. A total of 83 ectomycorrhizal species were collected under Abies holophylla, 66 species under Pinus densiflora, 50 species under Pinus rigida, 49 species under Pinus koraiensis, 46 species under Quercus aliena, and 23 fungal species under Larix leptolepis. Particularly, Larix leptolepis was associated with different fungal flora, with fewer species of Amanita, Lactarius and Russula, and more species of Laccaria. Most host tree species surveyerl in the present study appeared to have low fungus specificity for ectomycorrhizal formation.

• Korean Journal of Environment and Ecology
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• v.24 no.6
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• pp.657-679
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• 2010

Mushroom fruiting was investigated in pine and oak dominated forest stands in Songni National Park located in central Korea for six years from 2003 to 2008, in order to understand the relationship between mushroom diversity and the environmental factors, precipitation, temperature, soil moisture and vegetation. The most frequent fruiting families were those of ectomycorrhizal mushrooms, Tricholomataceae, Amanitaceae, Russulaceae, Cortinariaceae, and Boletaceae. The frequency of mushroom fruiting varied from 94 to 167 species per year, with July and August having the highest(13~90 species). Mushroom fruiting was positively correlated to precipitation(r=0.897), using Palmer Drought Severity Index for the long term period and Standard Precipitation Index for short term period. Soil moisture content also affected mushroom fruiting, with Lactarius chrysorrheus and Russula virescens fruiting only at soil moisture content higher than 20%. Positive correlation between mushroom fruiting and temperature was also noted(r=0.77), with optimum rates at $21{\sim}25^{\circ}C$. Tricholoma flayayirens, Amanita gymnopus, Lactarius piperatus, Inocybe asteropora and Xerocomus chrysenteron were able to fruit at temperatures higher than $25^{\circ}C$. However, Laccaria amethystea, Amanita virosa and Russula mariae fruited at relatively wide temperature range. The influence of vegetation on mushroom fruiting was likewise noted, with 38 species, including Suillus bovinus and Boletopsis leucomelas being specific to pine dominated stands, while 42 species, including Polyporus arcularius and Hericium erinaceum were specific to oak dominated stands. On the other hand, around 50 species, including Laccaria laccata and Lycoperdon parlatum, were able to fruit in both types of vegetation. In conclusion, mushroom fruiting greatly varies with changes in precipitation, soil moisture, temperature and vegetation.

• Archives of Pharmacal Research
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• v.5 no.1
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• pp.21-23
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• 1982

To develop new potent antitumor substancces with low toxicity from natural products, especially from higher fungi of Korea, the carpophores of some wild basidiomycetes were collected and the antitimor test for their polysaccharides was done. The dried carpophores were extracted with hot water, and from the extracts, crude polysaccharides were obtained by alcohol precipitation. The alcohol precipitated crude polysaccharides were partially purified by dialysis and then used as the samples for antitumor tests. Among tested, the polysaccharide of Laccaria laccata, Trametes sanguineus, Armlliariella mellea, Clitocybe in fundibuliformis, and Xeromphalina campanella respectively showed the inhibition ratio of 75%, 72%, 94% 55%, and 47% when administered i. p. once dailly for ten days at the dose level of 20mg/kg/day into the mice implanted with $10^{6}$ cells of sarcoma 180/mouse. However, those of Craterellus cornucopiodes, Daedaleopsis confrogosa, and Coriolus sp. showed almost no activities.

• The Korean Journal of Mycology
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• v.21 no.1
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• pp.64-72
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• 1993

Fresh fungi were collected during five field trips to Ullung Island from October of 1989 to September of 1991. Through the observation of some agaric fungi, thirty mushrooms were identified to the species and are listed as below. Among them, three species, Crepidotus geophilus, Mycena elegans, ana Mycena carolinensis were confirmed new to Korea and are registered here with descriptions.