• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.474-479
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• 2010

In this paper, we analyzed the microsatellites in the transcript sequences of the whole Laccaria bicolor genome. Our results revealed that, apart from the triplet repeats, length diversification and richness of the detected microsatellites positively correlated with their repeat motif lengths, which were distinct from the variation trends observed for the transcriptional microsatellites in the genome of higher plants. We also compared the microsatellites detected in the genic regions and in the nongenic regions of the L. bicolor genome. Subsequently, SSR primers were designed for the transcriptional microsatellites in the L. bicolor genome. These SSR primers provide desirable genetic resources to the ectomycorrhizae community, and this study provides deep insight into the characteristics of the micro satellite sequences in the L. bicolor genome.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2057-2063
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• 2018

Laccases can oxidize a variety of phenolic and non-phenolic substrates including synthetic dyes. In this research, a laccase gene Lcc9 from Laccaria bicolor was chemically synthesized and optimized to heterogeneous expression in Pichia pastoris and Arabidopsis thaliana. The properties of recombinant laccase expressed by P. pastoris were investigated. The laccase activity was optimal at 3.6 pH and $40^{\circ}C$. It exhibited $K_m$ and $V_{max}$ values of $0.565mmol\;l^{-1}$ and $1.51{\mu}mol\;l^{-1}\;min^{-1}$ for ABTS respectively. As compared with untransformed control plants, the laccase activity in crude extracts of transgenic lines exhibited a 5.4 to 12.4-fold increase. Both laccases expressed in transgenic P. pastoris or A. thaliana could decolorize crystal violet. These results indicated that L. bicolor laccase gene may be transgenically exploited in fungi or plants for dye decolorization.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.31-38
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• 2011

In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The SCAR primer pair 'S-OPA13-1' amplified a 590-bp fragment in the varieties originated from Suhan strain. The Blast search of S-OPA13-1 showed high homology to the POMFBO1 P. ostreatus cDNA clone MFB02-A05 and Laccaria bicolor S238N-H82. The results showed that this SCAR marker can clearly distinguish Suhan strains from Pleurotus spp.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.48-69
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• 1987

To understand host ranges and host specificity of ectomycorrhizal fungi and fungus specificity of host tree species, higher fungi from pure forest stands of following twelve tree species were collected for six years from 1981 to 1986: Pinus densiflora, P. rigida, P. koraiensis, Picea koraiensis, Larix leptolepis, Abies holophylla, Populus $alba{\times}glandulosa$, Castanea crenata, Quercus aliena, Q. acutissima, Q. mongolica, and Betula platyphylla. A total of 196 ectomycorrhizal species with 8 varieties in 48 genera were identified. Thirty-one species with one variety belonged to Russula, 22 species with 5 varieties to Amanita, 18 species with one variety to Lactarius. Fungi belonging to above three gepera, Laccaria, and Cantharellus had a relatively wide host range. Laccaria laccata was collected under all the 12 tree species, Amanita vaginata group under 11 tree species, Laccaria amethystina and Russula foetens under 10 tree species, Lactarius gerardii and Russula sororia under 9 tree species, and Amanita agglutinata, Cantharellus cibarius, Russula bella, and R. virescens under 8 tree species. Above 10 fungal species may be classified to have a wide host range. Following fungi were collected under 4 to 5 tree species of both conifers and broad-leaved trees: Amanita citrina, Boletus bicolor, B. erythropus, Lactarius piperatus, L. subzonar­ius, and Russula pseudodelica. Above fungi may be classified to have an intermediate host range. Chroogomphus, Gomphidius, Rhizopogon, and Suillus were collected only under Pinaceae and may be classified to have a narrow host range. Particularly Suillus grevillei was collected only under Larix leptolepis, and appeared to have high host specificity. A total of 83 ectomycorrhizal species were collected under Abies holophylla, 66 species under Pinus densiflora, 50 species under Pinus rigida, 49 species under Pinus koraiensis, 46 species under Quercus aliena, and 23 fungal species under Larix leptolepis. Particularly, Larix leptolepis was associated with different fungal flora, with fewer species of Amanita, Lactarius and Russula, and more species of Laccaria. Most host tree species surveyerl in the present study appeared to have low fungus specificity for ectomycorrhizal formation.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1055-1059
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• 2013

The ectomycorrhizal fungus Tricholoma matsutake grows symbiotically with Pinus densiflora. Phenylalanine ammonia-lyase (E.C. 4.3.1.24) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. The role of fungal phenylalanine ammonia-lyase, however, has not been clear until now. In this study, the gene encoding phenylalanine ammonia-lyase (PAL), which was isolated from T. matsutake, was cloned and characterized. The PAL gene (tmpal) consists of 2,160 nucleotides, coding for a polypeptide containing 719 amino acid residues. The deduced amino acid sequence of tmpal from T. matsutake shows high identity (70%) with that from Laccaria bicolor. Comparative analysis of the PAL genes among T. matsutake and other species of the class Agaricomycetes showed that both active sites and binding sites were significantly conserved among these genes. The transcriptional analysis of the PAL gene revealed a differential gene expression pattern depending on the developmental stages (mycelium, primordium, stipe, pileus, and gills) of T. matsutake. These results suggest that the PAL gene in T. matsutake plays an important role in multiple physiological functions.