The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Development of Suhan Strain-specific SCAR Marker in Pleurotus ostreatus

느타리 버섯에서 수한 품종 특이 SCAR marker 개발

  • Seo, Kyoung-In (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Soon-Young (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Kwang-Ho (Department of Crop Science, KonKuk University) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 서경인 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 박순영 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김광호 (건국대학교 식량자원학과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2011.03.21
  • Accepted : 2011.03.28
  • Published : 2011.04.01
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Abstract

In this study, 81 commercial strains of Pleurotus species cultivated in South Korea were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Sequence characterized amplified region (SCAR) markers were developed by designing from one RAPD polymorhic band specific to Suhan strain. The SCAR primer pair 'S-OPA13-1' amplified a 590-bp fragment in the varieties originated from Suhan strain. The Blast search of S-OPA13-1 showed high homology to the POMFBO1 P. ostreatus cDNA clone MFB02-A05 and Laccaria bicolor S238N-H82. The results showed that this SCAR marker can clearly distinguish Suhan strains from Pleurotus spp.

현재 70개 이상의 느타리 품종이 유통되어 재배되고 있다. 본 연구에서는 81개 품종을 수집하여 핵산지문법으로 분석하였다. 이중 수한과 그 유사품종에서 특이하게 나타나는 밴드를 이용하여 수한 품종에 특이적인 SCAR marker로 개발하였다. 수한 품종 특이 band에 대한 clone을 기존에 알려진 유전자 염기서열과 유사성이 있는지를 확인한 결과 POMFBO1 Pleurotus ostreatus cDNA clone MFB02-A05, mRNA sequence와 92%의 homology를 나타냈고, 등록된 아미노산 서열과의 유사성을 확인한 결과 큰졸각버섯인 Laccaria bicolor의 predicted protein과 가장 높은 sequence homology (BlastX score = 73.9, E value = $5e^{-25}$)를 보였다. 본 연구를 통하여 개발된 수한특이 마커는 정확한 품종구분이 요구되는 종균유통 과정에서 수한계통 품종을 구분하는 유용한 마커로 이용될 것으로 기대된다.

Keywords

References

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