• Molecules and Cells
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• v.42 no.1
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• pp.45-55
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• 2019

The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.996-1004
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• 2020

Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorption-desorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

• Journal of the Korean Institute of Educational Facilities
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• v.8 no.3
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• pp.25-35
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• 2001

This study investigated technology education, laboratory management and facilities in middle schools with the goal of finding problems. Ninety-six randomly selected schools and questionnaire was done to the teachers of 84 schools which have laboratory. Five of these schools received on-site inspections. The results of study are as follows : 1) 12.5% of schools have no laboratory. The average laboratory has less than 50% of the necessary equipment for total operations. laboratories are used an average of once or twice per two time frame. 2) 80%of the laboratories are located at the end of a row of regular class rooms. The most common laboratory style is classroom plus hallway($90m^2$) style. 77% of laboratories have a single entrance. 3) 69% of teachers reported the worktable arrangement to be inconvenience. 68% of the schools have 9 worktables per laboratory. 38% say the worktables are not wide enough and 36% said they were appropriate. Table dimensions range from $95{\sim}110cm$ in width and from $210{\sim}225cm$ in length. 65% of the teachers say table height is low with a range from $76{\sim}82cm$. 86% of the teachers say that the height of chair is low and the range of its measurement is from $37{\sim}47cm$. 84% of the teachers points out that laboratory is poorly lighted.

• Journal of the Korean Institute of Gas
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• v.16 no.5
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• pp.21-27
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• 2012

The loss of life and property due to accidents in the research facilities or the laboratories of the university occurs steadily and the necessity of laboratory accident prevention is proposed. Above all, the main work to laboratory accident prevention is a systematic analysis of laboratories accidents. Analyzing reports or researches on industrial accidents in Korea had been carried out but these researches or reports did not based on laboratory accidents analysis. To the establishment of the accident prevention countermeasure in laboratory, a questionnaire sheet has been developed in this study. The questionnaires to survey the accident cases were gathered by electronic mail and visit survey from the laboratories and universities. The data of accident cases from the questionnaires was analyzed and discussed on accident distribution by season, the type of accident classification, the type of occurrence, the objects that caused the accident and laboratory accident by the damage incurred etc.. These results of this study can be used as basic data to the safety security and laboratory accident prevention of the laboratory worker.

• Parasites, Hosts and Diseases
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• v.58 no.2
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• pp.161-171
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• 2020

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

• Animal Bioscience
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• v.36 no.6
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• pp.829-839
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• 2023

Objective: The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods: We obtained the ACADL gene of goats by cloning and used quantitative real-time polymerase chain reaction (qPCR) to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results: The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion: So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats.

• Journal of The Korean Association For Science Education
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• v.26 no.3
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• pp.342-355
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• 2006

The purpose of this study was to develop a classification scheme for laboratory instruction, which could occupy a central and distinctive role in science education. For this study, literature on laboratory instruction types were analyzed. Utilizing several of these theoretical frameworks, a Classification Scheme for Laboratory Instruction (CSLI), which clearly represents various features of laboratory instruction, was created. The developed CSLI consisted of two descriptors: one is the procedure for laboratory instruction, and the other is a way of approach. The procedure is either designed by the students or provided for them from an external source. A dichotomy also exists for the approach taken toward the activity: deductive or inductive. Validity was established for the CSLI. In addition, laboratory instruction according to CSLI was divided into four types: verification, discovery, exploratory, and investigation. Although this study demonstrated only limited features of laboratory instruction due to the absence of a field test, it serves as a model for more comprehensive studies.

• Journal of the Korean Society of Safety
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• v.31 no.4
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• pp.126-135
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• 2016

The purpose of this study is to develop risk assessment techniques and institutional analysis of domestic and international, the management techniques that can efficiently manage the harmful factor of the laboratory and to present the institutional measures that can be efficiently implemented. Due to a variety of adverse factors of laboratory, accidents of laboratory of various forms have occurred, but there is no risk assessment system in order to manage this effectively. So, we investigated this domestic existing risk assessment methods and the outside of the risk assessment system, and also analyzed accidents of domestic laboratory that occurred in 2014. In addition, we targeted the 24 laboratories in 21 universities to investigate the management of harmful factors of the laboratory and performed applying test for 12 domestic laboratories. Existing risk evaluation system, such as PMS, SMS, off-site impact assessment, since the industrial site is the subject, is a difficult problem to be directly applied to the laboratory of the research institute. So, we implemented management status and harmful factors survey and classified the research and development activities based on this data. Finally we developed "pre-hazards risk analysis method" to create each of the safety management measures. In addition, research activities personnel conducted voluntarily risk assessment, which is shared by institutions and government. It is presented the institutional system for safety management of laboratory. Its result, pre-hazards risk analysis method and institutionalization scheme will be able to achieve laboratory accident prevention system.

• Journal of Technologic Dentistry
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• v.35 no.4
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• pp.377-385
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• 2013

Purpose: This research conducted by using Department of Dental Laboratory Technology students investigates interpersonal and emotional intelligence impact on psychological happiness. Methods: Study was conducted in the Daegu-Gyeongbuk area Department of Dental Laboratory Technology students, based on 132 people who were enrolled college students. Data analysis, frequency analysis, factor analysis, reliability test, correlation analysis, multiple regression analysis was carried out and SPSS/WIN 18.0 was used. Results: Interpersonal factors utilize emotions, emotional self-control and emotional awareness. Others turned positive (+) was found to affect the psychological, emotional factors utilized in euphoria, emotional adjustment and were found to have positive effects. Conclusion: Looking at the above results gathered from dental engineering students, improvement in interpersonal relationships, and psychological welfare have improve more effectively through utilizing and controlling emotions, including the ability to set their own goals for the future. Motivational training programs for strengthening study habits is more important than what can be based in dental college engineering students. In conjunction with an active lifestyle, future professional dental health services for patients and clinicians care about the improvement of his own psychological happiness was inspire by the care received from home and school and the resulting map is considered to require constant attention.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2004.05a
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• pp.82-82
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• 2004