• 제목/요약/키워드: Laboratory and field culture

검색결과 83건 처리시간 0.019초

Laboratory, Field and Deep Seawater Culture of Eucheuma serra-a High Lectin Yielding Red Alga

  • Dinabandhu Sahoo;Masao Ohno;Masanori Hiraoka
    • ALGAE
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    • 제17권2호
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    • pp.127-133
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    • 2002
  • The red seaweed Eucheuma serra is a high yielding source of lectins. The plants were collected from a depth of 5-6 meters and cultured in the laboratory, field and deep seawater. A Daily Growht Rate (DGR) of 3.5% was observed at 18℃ with a low light of 30μmol photon $ m^{-2} · s^{_1}$ in the laboratory. When the plants were cultured in the field at different depths during winter onths of December and January, best growth was observed at 1 m depth and a DGR of 2.14±0.04% was recorded. The plants grown in the tank with a continuous supply of deep seawater showed a DGR of 8.2% The results indicate that E. serra can be cultivated in large scale both in deep seawater in the tank and in the field for the extraction of lectins at a commercial scale.

한국 야생 등줄쥐의 고해상도 염색체분염상 (Chromosomal band pattern of black-striped field mouse (Apodemus agrarius))

  • 오승현;윤여성;진희경;성제경
    • 대한수의학회지
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    • 제44권2호
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    • pp.159-162
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    • 2004
  • We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

VanB-vanA Incongruent VRE Isolated from Animals and Humans in 1999

  • Shin En-Joo;Hong Hyun-Gin;Ike Yasuyoshi;Lee Kyung-Won;Park Yong-Ho;Lee Dong-Taek;Lee Yeon-Hee
    • Journal of Microbiology
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    • 제44권4호
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    • pp.453-456
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    • 2006
  • 16 chicken isolates and four clinical isolates of VanB-vanA incongruent vancomycinresistant Enterococcus faecium strains without vanS were isolated in 1999. Pulsed-field gel electrophoresis revealed only a peripheral relationship between the chicken isolates and clinical isolates, but suggested clonal spread in the chicken isolates.

한국산 홍조 Antithamnionella miharae (Tokida) Itono (Antithamnionella miharae (Tokida) Itono (Rhodophyta, Ceramiaceae) in Korea)

  • 부성민
    • Journal of Plant Biology
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    • 제28권3호
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    • pp.199-206
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    • 1985
  • Antithamnion miharae (Tokida) Itono was investigated in field and laboratory culture. The plant grows in lower littoral zone through out the year in Korea. The gland cells occur scarcely or commonly according to the populations, but the terminal hairs in the female plants are not observed. The structure of vegetative thallus and the reproductive organs in culture are similar to those of field materials, except for the number of gonimoblasts, which become five to six in contrast to one to three of the field. A typical Polysiphonia-type life history is repeated in culture. This species which produces tetrahedral tetrasporangia is markedly distinguished from Antithamnion glandulifera sensu Kylin (=Antithamnionella glandulifera (Kylin) Wollaston) which produces cruciate tetrasporangia, while the plants reported by Dawson (1962) and Wollaston (1971) as A. glandulifera seem to be conspecific with A. miharae by the fact that they have the tetrahedral tetrasporangia in stead of cruciate ones.

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한국들잔디 배아세포의 부유배양과 식물체 재생 (Embryogenic cell suspension culture and plant regeneration in zoysiagrass (Zoysia japonica Steud))

  • 팽왼주안;한리바우;퀴충휘;리다이엥;박태윤
    • 아시안잔디학회지
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    • 제23권2호
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    • pp.345-352
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    • 2009
  • 한국들잔디는 운동경기장이나 골프 코스 등에 폭넓게 쓰이는 난지형 잔디이다. 많은 품종은 영양체로 번식한다. 이 연구는 배아세포 유도와 식물체 재생을 위한 적정 배양액 개발과 배양조건을 연구하여, 한국잔디의 육종과 번식을 위한 세포배양 체계 확보를 목적으로 하였다. 그 결과 $Cu^{++}\;2.5mgL^{-1}$를 유도 배양액에 첨가하는 것이 캘러스 유도를 위한 최적 농도 조건 이였음을 보여주었다. Sub-culture의 횟수의 증가는 캘러스의 질을 향상시켰다. 세포 부유배양을 위한 최적 용량은 2.5에서 10mL 사이였으며 본 연구에서 58%의 식물체 재생산비율을 보였다.

감염관리 분야에서 감시배양검사의 표준화 연구 (Study on the Standardization of a Surveillance Culture Laboratory in Infection Control Fields)

  • 박창은;정나연;양민지;김한울;주세익;김건한;성희경;황유연;임현미;손재철;윤선한;윤남섭;장인호
    • 대한임상검사과학회지
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    • 제50권3호
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    • pp.359-369
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    • 2018
  • 의료 관련 감염을 예방하기 위한 필수적 방법은 일관성이 있는 감염감시 시스템을 구축하고 효율적인 감시 통제를 수행하기 위해 신뢰할 수 있는 상황에 대해 진단을 향상시켜나가는 것이다. 또한 의료 종사자의 손과 의복 및 장비는 환자 관리 및 환경과 접촉하여 병원체가 오염되는 요인이다. 병원체를 가진 시설 표면(예: 침대 레일, 침대 옆 탁자), 음용수, 냉각탑용수, 내시경기구, 급식위생, 공기매개, 멸균검사, 내독소검사, 및 의료 장비를 포함한 의료 환경의 오염은 일반적으로 발생한다. 또한 이러한 감염원을 활동 감시를 통해 MLST, PFGE의 기법으로 역학분석을 수행한다. 따라서 HAI 예방을 위한 환경 감시배양 검사는 환자의 안전과 감염원의 차단을 향상시킬 뿐만 아니라 국가의 감염관리 시스템을 통제하여 최적의 효율적인 감염관리 예방을 가능케 하고 국가의 감염관리 시스템의 안전을 향상시킨다. 결론적으로 감시배양 검사의 표준화를 통해 실효성 있는 감염관리체계에 이바지하고 감염관리 전문인력으로서의 전문성을 확보하고자 한다. 이를 통해 표준화 마련의 일차적인 목표는 의료관련 감염을 줄이고 국가적 의료관리 체계를 향상시키는데 있다.

자동차 창유리의 가시광선 투과율에 따른 운전반응의 정확도 (The Effects of Auto-mobile Tinting upon Driver's Responses)

  • 한덕웅;박군석
    • 한국심리학회지 : 문화 및 사회문제
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    • 제12권4호
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    • pp.77-99
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    • 2006
  • 자동차 창유리의 가시광선 투과율을 규제하기 위한 기준에 관한 기초연구로 두 개의 실험실 실험, 현장실험 및 실제로 교통사고를 조사한 현장조사를 포함하여 모두 4개 연구를 수행하였다. 이 가운데 실험실 연구들에서 가시광선 투과율이 100%인 조건에 비하여 65%, 50%, 30%인 세 조건에서 모두 오경보 반응이 유의하게 높았으며, 50% 가시광선 투과율 조건에서 시력이 0.7 이하인 운전자들은 0.7 이상인 운전자들에 비하여 누락 반응이 유의하게 높았다. 현장실험에서는 가시광선 투과율이 80%인 조건보다 50%와 30%의 가시광선 투과율 조건에서 각각 돌출하는 자극을 지각하는 오답수가 높았다. 교통사고를 조사한 현장연구에서도 80% 가시광선 투과율의 자동차를 운전하는 사람들의 사망/상해 사고빈도에 비하여 65% 조건에서 비율이 높았다. 이 연구 결과들을 근거로 가시광선 투과율을 규제하는 기준을 70%로 설정하도록 제안하고, 현실을 감안하여 이 기준을 점진적으로 적용하는 전략을 제시하였다.

Best Practices on Improving the Virtual Reality (VR) Content Development Process with EPIC's Unreal Engine

  • Kong, Ji Hoon;Kim, Ki Du;Kim, R. Young Chul
    • International Journal of Advanced Culture Technology
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    • 제9권4호
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    • pp.417-423
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    • 2021
  • Recently, in the Game industries, they are increasing to use of game engines to reduce the development cost of 3D content and software. In particular, Unreal Engine provides a blueprint visual scripting function that enables software production without programming (coding). Although High-end video content can be produced, the problem is that content development is complicated and requires advanced manpower. To solve this problem, we propose an optimized VR game context process. This is because 1) a Blueprint visual script is used, 2) VR games with various interactions can be produced, 3) Non-majors in the software field (or groups) can develop advanced content. In various related industries such as defense, medical care, manufacturing, and construction, we may easily develop any game content without programming with our refined VR rhythm action game development process. We expect to reduce the development cost with the process advantages in the game industries.

Mass Propagation of Vitex negundo L., in vitro

  • Thiruvengadam, Muthu;Jayabalan, Narayanasamypillai
    • Journal of Plant Biotechnology
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    • 제2권3호
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    • pp.151-155
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    • 2000
  • Shoot proliferation was obtained from shoot tips and nodal explants of Vitex negundo L. on MS medium supplemented with either BAP or KIN (0.1-2.0 mg/L) alone or in combination with NAA (0.1 mg/L). The concentrations of cytokinins combined with NAA produced multiple shoots from shoot tips and nodal explants. The highest mean percentage (84.3$\pm$8.0) of shoot multiplication's were observed on nodal explants in the presence of BAP (1.5 mg/L) and NAA (0.1 mg/L) followed by shoot tips (65.0$\pm$5.0). The regenerated shootlets were rooted on MS basal medium IAA, IBA, NAA (0.1-1.5 mg/L). The maximum number of roots (51.0$\pm$2.6) was achieved on the medium containing IBA (1.0 mg/L) followed by other auxins (NAA, IAA). The regenerated plants were successfully transferred to a mixture of vermiculate and soil. About 95% of the plantlets survived when transferred to the field.

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홍조 비단풀속 식물의 실내부양에 따른 형태변화와 생식에 관하여 (Morphology and Reproduction of Some Species of Ceramium (Rhodophyta) in Culture)

  • Suh, Youngbae
    • Journal of Plant Biology
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    • 제27권3호
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    • pp.163-171
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    • 1984
  • Ceramium kindoi Yendo demonstrates a Polysiphonia type of life history without diviation in unialgal culture. However, cultures of C. paniculatum Okamura and C. aduncum Nakamura from Kangneung shows considerable phenotypic variations in laboratory. In C. paniculatum, the subulate spines disappear, the transverse cell-rows of corticating bands decrease in number, and the growing direction of corticating cells is changed during the culture. In C. aduncum, a species new to Korea, frond apices become slightly incurved in contrast to strongly rolled ones in the field, and the rows of corticating cells decrease in number and height during the culture. These results suggest that some significant taxonomic characters currently used for identification of Ceramium species are reconsidered.

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