• ALGAE
• /
• v.17 no.2
• /
• pp.127-133
• /
• 2002

The red seaweed Eucheuma serra is a high yielding source of lectins. The plants were collected from a depth of 5-6 meters and cultured in the laboratory, field and deep seawater. A Daily Growht Rate (DGR) of 3.5% was observed at 18℃ with a low light of 30μmol photon $ m^{-2} · s^{_1}$ in the laboratory. When the plants were cultured in the field at different depths during winter onths of December and January, best growth was observed at 1 m depth and a DGR of 2.14±0.04% was recorded. The plants grown in the tank with a continuous supply of deep seawater showed a DGR of 8.2% The results indicate that E. serra can be cultivated in large scale both in deep seawater in the tank and in the field for the extraction of lectins at a commercial scale.

• Korean Journal of Veterinary Research
• /
• v.44 no.2
• /
• pp.159-162
• /
• 2004

We investigated the cytogenetic characteristics of male black-striped field mouse (Apodemus agrarium) in Korea. Chromosome slides were obtained from blood cell cultures which were synchronized with thymidine blocking or not. In the chromosome slide which synchronization with thymidine blocking was employed on, the GTG(G bands by trypsin using Giemsa)-bands of high resolution were observed. The male black-striped field mouse has 48 chromosomes composed 46 autosomes and XY sex chromosomes. The centromeric regions of autosomes were positive to GTG-banding. According to this investigation, thymidine blocking in cell culture process was useful to get lengthened chromosomes. It may be necessary to employ RBG-banding technique to investigate complementary band patterns between R- and G-banding in black-striped field mouse.

• Journal of Microbiology
• /
• v.44 no.4
• /
• pp.453-456
• /
• 2006

16 chicken isolates and four clinical isolates of VanB-vanA incongruent vancomycinresistant Enterococcus faecium strains without vanS were isolated in 1999. Pulsed-field gel electrophoresis revealed only a peripheral relationship between the chicken isolates and clinical isolates, but suggested clonal spread in the chicken isolates.

• Journal of Plant Biology
• /
• v.28 no.3
• /
• pp.199-206
• /
• 1985

Antithamnion miharae (Tokida) Itono was investigated in field and laboratory culture. The plant grows in lower littoral zone through out the year in Korea. The gland cells occur scarcely or commonly according to the populations, but the terminal hairs in the female plants are not observed. The structure of vegetative thallus and the reproductive organs in culture are similar to those of field materials, except for the number of gonimoblasts, which become five to six in contrast to one to three of the field. A typical Polysiphonia-type life history is repeated in culture. This species which produces tetrahedral tetrasporangia is markedly distinguished from Antithamnion glandulifera sensu Kylin (=Antithamnionella glandulifera (Kylin) Wollaston) which produces cruciate tetrasporangia, while the plants reported by Dawson (1962) and Wollaston (1971) as A. glandulifera seem to be conspecific with A. miharae by the fact that they have the tetrahedral tetrasporangia in stead of cruciate ones.

• Asian Journal of Turfgrass Science
• /
• v.23 no.2
• /
• pp.345-352
• /
• 2009

Zoysiagrass (Zoysia japonica Steud) is a warm season turfgrass species widely used for sports field and golf courses. Many cultivars are propagated through vegetative methods. This study was conducted to develop an optimum culture medium and culture conditions for embryogenic callus induction and plant regeneration, and to establish a cell suspension culture system for use in zoysiagrass breeding and propagation. The results indicated that adding $Cu^{++}$ at 2.5 mg $L^{-1}$ to the induction medium was optimum for callus induction. Increasing the numbers of sub-culture cycles improved the quality of calli. The optimum dosage for cell suspension culture ranged from 2.5 to 10 mL. The embryogenic callus suspension used in this study had a plant regeneration rate of 58%.

• Korean Journal of Clinical Laboratory Science
• /
• v.50 no.3
• /
• pp.359-369
• /
• 2018

An essential measure to prevent healthcare-associated infections (HAI) is to develop a consistent system of surveillance, thereby promoting a reliable situation diagnosis to perform efficient control for the problem. Patient-to-patient transmission of pathogens within the hospital plays a substantial role in the epidemiology of HAIs. Contamination of healthcare environments commonly occurs, including facilities surfaces (e.g., bed rails, bedside tables), drinking water, cooling tower water, endoscopic instruments, food, airborne, endotoxin test, sterile test and medical equipment, with pathogenic organisms. In addition, epidemiological analysis is performed by multi locus sequence tying, pulsed-field gel electrophoresis for active surveillance. Therefore, an environmental surveillance culture test for prevention improves patient safety and blocks infection agents. Effective infection control and increased safety are possible by controlling the national infection control system. In conclusion, this study contributes to an effective infection control system through the standardization of active surveillance culture laboratory and secure expertise as infection control specialist. The primary objective of the standardization is to improve the safety of the nation's healthcare system by reducing the rates of HAIs.

• Korean Journal of Culture and Social Issue
• /
• v.12 no.4
• /
• pp.77-99
• /
• 2006

The purpose of this study was to find out the effects of auto-mobile tinting upon driver's cognitive responses and behaviors through two laboratory experiments, a field experiment and a traffic accident data. The results of two laboratory experiments showed that there were higher false alarm responses under the conditions of 65%, 50%, 35% tinting level than thoses under the 100% level condition. It was also shown that the drivers who had bad sight made more missing responses than the drivers who had normal vision. The main results of the laboratory experiment were repliceted through both the field experiment and the survey research of car accidents. The results of this study were discussed in terms of the previous studies performed abroad. We strongly suggested 70% tinting level as a regulation standard for safe driving and the strategies for implementing the regulation rule.

• International Journal of Advanced Culture Technology
• /
• v.9 no.4
• /
• pp.417-423
• /
• 2021

Recently, in the Game industries, they are increasing to use of game engines to reduce the development cost of 3D content and software. In particular, Unreal Engine provides a blueprint visual scripting function that enables software production without programming (coding). Although High-end video content can be produced, the problem is that content development is complicated and requires advanced manpower. To solve this problem, we propose an optimized VR game context process. This is because 1) a Blueprint visual script is used, 2) VR games with various interactions can be produced, 3) Non-majors in the software field (or groups) can develop advanced content. In various related industries such as defense, medical care, manufacturing, and construction, we may easily develop any game content without programming with our refined VR rhythm action game development process. We expect to reduce the development cost with the process advantages in the game industries.

• Journal of Plant Biotechnology
• /
• v.2 no.3
• /
• pp.151-155
• /
• 2000

Shoot proliferation was obtained from shoot tips and nodal explants of Vitex negundo L. on MS medium supplemented with either BAP or KIN (0.1-2.0 mg/L) alone or in combination with NAA (0.1 mg/L). The concentrations of cytokinins combined with NAA produced multiple shoots from shoot tips and nodal explants. The highest mean percentage (84.3$\pm$8.0) of shoot multiplication's were observed on nodal explants in the presence of BAP (1.5 mg/L) and NAA (0.1 mg/L) followed by shoot tips (65.0$\pm$5.0). The regenerated shootlets were rooted on MS basal medium IAA, IBA, NAA (0.1-1.5 mg/L). The maximum number of roots (51.0$\pm$2.6) was achieved on the medium containing IBA (1.0 mg/L) followed by other auxins (NAA, IAA). The regenerated plants were successfully transferred to a mixture of vermiculate and soil. About 95% of the plantlets survived when transferred to the field.

• Journal of Plant Biology
• /
• v.27 no.3
• /
• pp.163-171
• /
• 1984

Ceramium kindoi Yendo demonstrates a Polysiphonia type of life history without diviation in unialgal culture. However, cultures of C. paniculatum Okamura and C. aduncum Nakamura from Kangneung shows considerable phenotypic variations in laboratory. In C. paniculatum, the subulate spines disappear, the transverse cell-rows of corticating bands decrease in number, and the growing direction of corticating cells is changed during the culture. In C. aduncum, a species new to Korea, frond apices become slightly incurved in contrast to strongly rolled ones in the field, and the rows of corticating cells decrease in number and height during the culture. These results suggest that some significant taxonomic characters currently used for identification of Ceramium species are reconsidered.