• Title/Summary/Keyword: Labelling technique

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A Study on Recognition of Korean Connected-Digits Using group-labelling by VQ and One-stage DP (벡터양자화에 의한 group-labelling과 1-段 DP를 이용한 한국어 연결 숫자음 인식에 관한 연구)

  • Kim, Ja-Ryong;Choi, Kap-Seok
    • The Journal of the Acoustical Society of Korea
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    • v.10 no.2
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    • pp.5-12
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    • 1991
  • In this paper, we have recognized Korean connected-digits. To reduce the processing time the method of combining VQ one-stage DP is introduced and to reduce the number of reference pattern a new group labelling technique is proposed. Group-labelling is a method to segment and label connected-digit into groups by VQ. As experimental results with 3 connected-digits, the number of reference patterns are reduced in proportion to the number of groups which are labelled.

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Reserach of a Labelling Technique for Using 99mTc as an Adsorbable Radiotracer for Hydrodynamics Studies of Fine Sediments in Suspension (미세부유사의 수력학적 연구 활용을 위한 흡착성 방사성표지물로서의 99mTc 제조기법 연구)

  • Jung, Sung-Hee;Kim, Jong-Bum;Moon, Jinho;Hong, Young-Don
    • Journal of Radiation Industry
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    • v.4 no.1
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    • pp.13-17
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    • 2010
  • The radioisotope labelling study was carried out for the sediment transport investigation. The fate of suspended solid materials is deeply related to the harbour siltation and the shoreline deformation that take place as a result of the artificial development of coastal area. In the experiment, $^{99m}TcO_4{^-}$ was chemically reduced and labelled in such a way that the labelled particles have the similar settling characteristics with the natural sediment. The radioisotope labelling techniques can be widely used for the natural resource exploration where the hydraulic dynamics of underground water and surface water are of importance.

Efficient Preparation of Radioiodine Labelled 3,5,3'-Triiodothyronine and Thyroxine for Medical Use

  • Kim, Jaerok;Kim, Tae-Ho
    • Nuclear Engineering and Technology
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    • v.7 no.2
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    • pp.127-133
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    • 1975
  • For isotopic exchange labelling of 3,5,3'-triiodothyronine (T$_3$) and thyroxine (T$_4$) with radioiodide in the presence of molecular iodine, T$_3$:I$_2$ or T$_4$:I$_2$ molar ratios, pH, and reaction time are. considered to be important factors. A modified labelling and separation method is proposed in present paper, by which T$_3$-$^{125}$ I and T$_4$-$^{125}$ I can be obtained with the mean labelling yields of 45%, and 50%, respectively. The whole reaction products can be separated by adoption of thin-layer chromatography technique using silica gel plate and the solvent system composd of chloroform, methanol and ammonia.

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Progression of Apoptotic Cells by Pretreatment of Proteinase K

  • Joo, Kyeng-Woong
    • Biomedical Science Letters
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    • v.8 no.3
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    • pp.161-165
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    • 2002
  • Apoptosis can be difficult to detect in routine histological sections. Since extensive DNA fragmentation is an important characteristic of this process, visualization of DNA breaks could greatly facilitate the identification of apoptotic cells. Several techniques for the qualitative and quantitative detection of this process have been established; recently, an in situ nick end-labelling technique based on the detection of DNA fragmentation, which is a molecular characteristic of apoptotic cell death, was described. Applying this method to paraffin sections of rat tissues, sensitivity was observed to be inconsistently low with regard to the expected number of apoptotic cells. I describe a new modified method for formalin-fixed, paraffin-embedded tissue sections, pretense pretreatment to permeate the tissue sections that involves an TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) is acknowledged as a method of choice in the rapid identification and quantification of the apoptotic cell fraction in paraffin tissue preparations. TUNEL was performed without apoptosis and with apopotosis samples to each of the three concentrations of proteinase K (10, 25, 40 mg/ml) pretreatments. In this study, I show that chemical pretreatments of the tissue sections in proteinase K (25 mg/ml for 15 min at room temperature) considerably enhances the sensitivity of this nick end labelling technique.

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The Evaluation of Factors which influence Binding Efficiency of Modified in Vivo Erythrocyte Labeling Technique (변형 체내 표지법에 의한 적혈구 표지시 결합효율에 영향을 미치는 인자 평가)

  • Seo Han-Kyung
    • Journal of The Korean Radiological Technologist Association
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    • v.30 no.1
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    • pp.63-69
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    • 2004
  • Purpose : We underwent this study to evaluate the factors which influence labelling efficiency when modified in vivo erythrocyte labeling technique was used. Materials and methods : Thirty healthy volunteers (M : F = 19 : 11, age : 25$\pm$2yrs)

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A Study on the Assessment of Hazardous Properties of the Chemicals (화학물질의 위험성평가에 관한 연구)

  • Lee, Bong-Woo;Park, Chul-Woo;Song, Haak;Choi, Jeong-Soo
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 2008.11a
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    • pp.262-269
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    • 2008
  • Chemical products have had an favorable influence on our everyday life, and contributed very much to the development of human culture. According to the rapid change of industry and the development of scientific technique the using chemical products are increasing more and more. Chemical products can have any hazardous property such as flammability or explosiveness. There are occurring many accidents in the international trade due to the different classification and labelling of chemicals produced in various countries. The main purpose of this work is the development of global standard test methods for the chemicals, and the classification and labelling in building block approach by means of the basic technical data. Oxidizing solids, combustible solids, spontaneously combustible materials, water-prohibitive materials, flammable liquids, self-reactive materials and oxidizing liquids have been tested. The results have been classified according to the hazard material safety regulation and the UN regulation, and summarized in a data-base.

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Efficient Character Segmentation Technique in the Natuaral Images Containing Character Sequences (문자열을 포함하는 자연 영상에서의 효과적인 문자 추출 기법)

  • Kim, Jong-Ho;Park, Sang-Hyun;Kang, Eui-Sung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.907-910
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    • 2011
  • This paper proposes a character segmentation algorithm of steel plate images composed of adaptive binarization by the SCW (Sliding Concentric Windows) technique, the object labelling by CCA (Connected Component Analysis), and 2D projection method. The SCW technique carries out the grayscale-to-binary image conversion in consideration of local characteristics of images. The character decision algorithm followed by the labelling technique by CCA (Connected Component Analysis) determines the character area effectively reducing the noise effect. The 2D projection with horizontal and vertical directions produces a tight bounding box for a character based on the cross points. Experimental results indicate that the proposed algorithm segments the characters in steel plate images effectively. The proposed algorithm can be applied to the devices with limited resources due to its excellent performance and low complexity.

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Radioisotope Labelling Method of Pine Gall Midge (Thecodiplosis japonensis UCHIDA et INOUYE) (솔잎혹파리에 대한 방사선동위원소 표식방법에 관한 연구)

  • Kwon S. H.;Chung K. H.;Ryu J.
    • Korean journal of applied entomology
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    • v.17 no.3 s.36
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    • pp.155-160
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    • 1978
  • This experiment was carried out to develope a technique for labelling pine gall midge with ·radioactive phosphorus and calcium. This technique evolved would help to obtain basic information on the appropriate concentration of $^{32}P\; and\; ^{45}Ca$ in tagging and determination of adult flight ranges which considered as one of the factors in dissemination. 1. Labelling at the larval stage of pine gall midge by injection of $^{45}Ca$ into pine tree trunk appeared not to be effective for tagging the adults because radioactivity was gradually decreased to background by emergence. 2. The radioactivities of larvae in the galls gradually increased in September but from October 1 the activity was gradually decreased for 10 days then equilibrium was maintained thereafter. The results seem to indicate that larval diet termination occured early October. 3. A maximum level of radiation was detected at 3 weeks after $^{32}P$ injection and thereafter it was gradually reduced. This result implies that $^{32}P$ is not appropriate radioisotope in labelling pine gall midge through trunk injection method. 4. After washing in running water for 10 minutes, the optimum$^{32}P$ concentration for treated overwintered larvae and pupae was found to be 0.5 micro curie per ml. for 30 minutes dipping.

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Inflammation Scan Using $^{99m}Tc-HMPAO$ Labelled Leukocytes ($^{99m}Tc-HMPAO$를 이용한 자가백혈구표지 및 그를 이용한 염증병소의 스캔)

  • Yang, Woo-Jin;Chung, Soo-Kyo;Shinn, Kyung-Sub;Bahk, Yong-Whee;Kim, Hoon-Kyo
    • The Korean Journal of Nuclear Medicine
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    • v.23 no.2
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    • pp.219-223
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    • 1989
  • Inflammation scan using radiolabelled leukocytes has high sensitivity and specificity. Several methods for labelling leukocytes have been evaluated using P-32 diisopropyl fluorophosphate (DFP-32), H-3 thymidine, Cr-51 chromate, Ga-67 citrate and Tc-99m-sulfur colloid. In-111-oxine has proved so far to be the most reliable agent for labelling leukocytes. In-111-oxine is, however, expensive, not easily available when needed, and its radiation dose to leukocytes is relatively high. Moreover, resolution of the resultant image is relatively poor. Tc-99m is still the agent of choice because of, as compared with the indium, its favorable physical characteristics, lower cost and availability. Now the technique for labelling the leukocytes with technetium is successfully obtained using the lipophilic HAPAO with higher efficiency for granulocytes than for other cells. With this technique it is possible to label leukocytes in plasma to improve the viability of the leukocytes. Inflammation scan using Tc-99m-HMPAO has been evaluated in several laboratories, and difference in methods for separation and labelling accounts for difference in efficiency, viability and biodistribution of the labelled leukocytes. We performed inflammation scan using leukocytes labelled with Tc-99m-HMPAO in three dogs 24 hours after inoculation of live E. Coli and A. Aureus in their right abdominal wall. We separated mixed leukocytes by simple sedimentation using 6% hetastarch (HES) and labelled the leukocytes with Tc-99m-HMPAO in 20% cell free plama diluted with phosphate buffer solution(Fig. 1). Uptake was high in the liver and spleen but is was minimal in the lungs on whole body scan. Kidneys and intestine showed minimal activity although it was high in the urinary bladder(Fig. 2). Uptake of labelled leukocytes in the inflammation site was do(mite on 2 hour-postinjection scan and abscess was clearly delineated on 24 hour-delayed scan with high target-to-nontarget ratio(Fig. 3, 4). Inflammation scan using mixed leukocytes labelled with Tc-99m-HMPAO is very sensitive and specific in early detection of inflammation.

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