• The Journal of the Korea Contents Association
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• v.9 no.11
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• pp.262-270
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• 2009

$^{18}F$-mefway has been developed as radioligand for serotonin receptor 5-$HT_{1A}$. The object of this study was to obtain the mefway precursor with the higher yield than previous method and to identify whether $^{18}F$-mefway can bind to 5-$HT_{1A}$ or not. from microPET imaging of small animal brain. Precursor was prepared by a modification of the reported procedure then [$^{18}F$] labeling was performed by adding $^{18}F$ ion at $130^{\circ}C$ in the hot cell for 30min. After purification of reaction mixture using alumina Sep-pak and HPLC, microPET images of small animal brain were determined. The chemical yield of precursor was increased from 9% to 34% using oxalyl chloride and LAH/diethylether. We synthesized a precursor which was successfully labeled with no-carrier-added $^{18}F$-by new synthetic route. This research suggest that $^{18}F$-mefway will be used a radiopharmaceutical for evaluation of central nerve system disorder as imaging a gent for 5-$HT_{1A}$ receptor.

• The Korea Journal of Herbology
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• v.31 no.1
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• pp.7-15
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• 2016

Objectives : Polygalae Radix (POL) has an ameliorating effect on learning and memory impairment caused by cerebral hypoperfusion. In regard to POL's action mechanism, this study was carried out to investigate the effects of POL on oxidative damage and neuronal apoptosis induced by cerebral hypoperfusion in rats.Methods : The cerebral hypoperfusion was induced by permanent bilateral common carotid artery occlusion (pBCAO) in Sprague-Dawley rats. POL was administered orally once a day (130 mg/kg of water-extract) for 28 days starting at 4 weeks after the pBCAO. Superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in the brain tissue were measured using ELISA method. Expressions of 4-hydroxynonenal (4HNE) and 8-hydroxy-2'- deoxyguanosine (8-OHdG) were observed using immunohistochemistry. In addition, neuronal apoptosis was evaluated with Cresyl violet staining, TUNEL labeling, and immunohistochemistry against Bax and caspase-3.Results : POL treatment significantly increased SOD activities and significantly reduced MDA levels in the cerebral cortex. The up-regulations of 4HNE and 8-OHdG expression caused by pBCAO in the CA1 of hippocampus were significantly attenuated by POL treatment. POL treatment also restored the reduction of CA1 thickness and CA1 neurons caused by pBCAO and significantly attenuated the apoptotic markers including TUNEL-positive cells, Bax, and caspase-3 expression in the CA1 of hippocampus.Conclusions : The results show that POL attenuated the oxidative damage in brain tissue and neuronal apoptosis in the hippocampus caused by the cerebral hypoperfusion. It suggests that POL can be a beneficial medicinal herb to treat the brain diseases related to cerebral hypoperfusion.

• The Journal of the Acoustical Society of Korea
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• v.14 no.2
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• pp.101-112
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• 1995

In order to construct a feature map-based phoneme classification system for speech recognition, two procedures are usually required. One is clustering and the other is labeling. In this paper, we present a phoneme classification system based on the Kohonen's Self-Organizing Feature Map (SOFM) for clusterer and labeler. It is known that the SOFM performs self-organizing process by which optimal local topographical mapping of the signal space and yields a reasonably high accuracy in recognition tasks. Consequently, SOFM can effectively be applied to the recognition of phonemes. Besides to improve the performance of the phoneme classification system, we propose the learning algorithm combined with the classical K-mans clustering algorithm in fine-tuning stage. In order to evaluate the performance of the proposed phoneme classification algorithm, we first use totaly 43 phonemes which construct six intra-class feature maps for six different phoneme classes. From the speaker-dependent phoneme classification tests using these six feature maps, we obtain recognition rate of $87.2\%$ and confirm that the proposed algorithm is an efficient method for improvement of recognition performance and convergence speed.

• The KIPS Transactions:PartD
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• v.13D no.7 s.110
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• pp.1017-1026
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• 2006

Recently, distributed collaborative development environment has been recognized an alternative environment for product development in which multidisciplinary participants are naturally involving. Reuse of Product design information has long been recognized as one of core requirements for efficient product development. This paper addresses an image-based retrieval system to support product design information reuse. In the system, product images obtained from multi-modal devices are utilized to reuse design information. The proposed system conducts the segmentation of a product image by using a labeling method and generates an attributed relational graph (ARG) that represents properties of segmented regions and their relationships. The generated ARG is extended by integrating corresponding part/assembly information. In this manner, the reuse of assembly design information using a product image has been realized. The main advantages of the presented system are following. First, the system is not dependent to specific design tools, because it utilizes multimedia images that can be obtained easily from peripheral devices. Second ratio-based features extracted from images enable image retrievals that contain various sizes of parts. Third, the system has shown outstanding search performance, because we applied various information of segmented part regions and their relationships between parts.

• The Korean Journal of Food And Nutrition
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• v.20 no.2
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• pp.226-239
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• 2007

The purpose of this study was to identify the problems related to the purchasing processes of school foodservices that should be corrected for the food service safety, by examining the purchasing processes and the status of supplier selection. A questionnaire was given to 300 dietitians working at self-operated food services. Ninety-eight responses, excluding incomplete answers, were used for the statistical analysis. The survey consisted of three parts: the general characteristics of the school foodservice and dietitian, purchasing processes and supplier selection, and the purchase specifications. We found that 84% of the contract was made by informal purchasing, and the contract period was 6 months or one year. For supplier selection, problems related to the document screening systems were the superficiality of the content(45.7%) and the absence or lack of clarity of the appraisal criteria(34.8%). The important factors for the facility and equipment standards of suppliers were included unclear evaluation methods for content(41.1%) and inappropriate appraisal lists(21.1%), while unclear evaluation methods for content(41.9%) and absence or lack of clarity of the appraisal criteria(20.4%) were the problems pertaining to the supplier evaluation checklist. When using the Food Labeling Standards to select suppliers, confirmation of the sell-by date and the storage method had the highest score at 3.85 out of 5. For supplier selection, only 25% of the contract was made by using the purchase specifications. The levels of satisfaction of with Kimchi and rice cakes suppliers were significantly different according to employment type and educational background, respectively. Depending on working experiences, satisfaction was significantly different for the use of document screening, as a standard for the selection and management of suppliers, and for the facility and equipment standards of suppliers, The use of purchase specifications was different by employment type, while the use of purchase specifications for contracts was different by working experience. These results imply that the specialization of suppliers is necessary to unsure food safety. Therefore, the objective methods to evaluate the suppliers should be developed by the government, and appropriate education programs for dietitians should be prepared to enhance the utilization of purchase specifications.

• Korean Journal of Remote Sensing
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• v.31 no.5
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• pp.395-408
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• 2015

High-resolution satellite image mosaics are becoming increasingly important in the field of remote sensing image analysis as an essential image processing to create a large image constructed from several smaller images. In this paper, we present an automatic seamline extraction technique and the procedure to generate a mosaic image by this technique. For more effective seamline extraction in the overlap region of adjacent images, an NDVI-based seamline extraction technique is developed, which takes advantage of the computational time and memory. The Normalized Difference Vegetation Index(NDVI) is an index of plant "greeness" or photosynthetic activity that is employed to extract the initial seamline. The NDVI can divide into manmade region and natural region. The cost image is obtained by the canny edge detector and the buffering technique is used to extract the ranging cost image. The seamline is extracted by applying the Dijkstra algorithm to a cost image generated through the labeling process of the extracted edge information. Histogram matching is also conducted to alleviate radiometric distortion between adjacent images acquired at different time. In the experimental results using the KOMPSAT-2/3 satellite imagery, it is confirmed that the proposed method greatly reduces the visual discontinuity caused by geometric difference of adjacent images and the computation time.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.31 no.4
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• pp.331-339
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• 2013

LiDAR has been one of the widely used and important technologies for 3D modeling of ground surface and objects because of its ability to provide dense and accurate range measurement. The objective of this research is to develop a method for automatic detection and modeling of railroad power lines using high density LiDAR data and RANSAC algorithms. For detecting railroad power lines, multi-echoes properties of laser data and shape knowledge of railroad power lines were employed. Cuboid analysis for detecting seed line segments, tracking lines, connecting and labeling are the main processes. For modeling railroad power lines, iterative RANSAC and least square adjustment were carried out to estimate the lines parameters. The validation of the result is very challenging due to the difficulties in determining the actual references on the ground surface. Standard deviations of 8cm and 5cm for x-y and z coordinates, respectively are satisfactory outcomes. In case of completeness, the result of visual inspection shows that all the lines are detected and modeled well as compare with the original point clouds. The overall processes are fully automated and the methods manage any state of railroad wires efficiently.

• Applied Microscopy
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• v.31 no.1
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• pp.1-8
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• 2001

The morphological features of neuronal dendritic spines are changed their shapes, sizes and density in response to physiological or pathological conditions . Therefore, exact analysis of spines warrants understanding of neuronal function. The size of the spine is at the borderline of resolution with light microscopy. High voltage electron microscopy Provide excellent resolution of the spines with proper stain techniques thanks to its higher resolution and penetration power. We evaluated more effective staining method for observing dendritic spines after labeling Purkinje cells with anti-calbindin 28 kD immunohistochemistry or Golgi staining methods. 4 fm thickness sections were observed with high voltage electron microscopy and some morphometric analyses were performed. Both Golgi staining and immunohistochemistry revealed the detail structures of the Purkinje cell such as soma, dendrites, and dendritic spines. High voltage electron micrographs with Golgi staining provide more precise morphology and are easy to measure. Average density of spine is $24.5{\pm}3.6/10{\mu}m$ and its length is $1.12{\pm}0.22{\mu}m$. For quantitative analysis of the spines, high voltage electron, micrographs with Golgi staining are more effective. This preliminary result is expected to be useful for further study of spine plasticity in various conditions.

• Journal of dental hygiene science
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• v.18 no.2
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• pp.76-84
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• 2018

Wet wipes are being increasingly used because of their convenience. Particularly, oral wet wipes are useful for regular cleaning of a baby's mouth after birth. Therefore, the consumption of oral wet wipes has increased over the past few years and a variety of products are commercially available. However, product information on safety is not sufficiently provided and still raises doubts regarding adverse effects. To confirm the safety of wet wipes as an oral hygiene item and provide information for their use, we investigated the cytotoxicity of oral wet wipes and verified the underlying mechanism. The anti-bacterial effect of oral wet wipes was analyzed using the disk diffusion method. The cytotoxic effects of oral wet wipes were observed based on morphological changes using microscopy and determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in gingival epithelial cells and gingival fibroblasts. Evaluation of apoptosis by oral wet wipes was explored using propidium iodide flow cytometric analysis and a terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) assay. Apoptosis-related molecules were also analyzed using western blotting. Five types of oral wet wipes were tested, and two products from Fisher-Price and Dr. Kennedy revealed strong cytotoxic effects on gingiva epithelial cells and gingiva fibroblasts, although they also showed intense anti-bacterial effects on oral bacteria. Cell cycle arrest in the G2/M phase and apoptosis were observed based on treatment of extracts from Fisher-Price and Dr. KENNEDY. Relatively high TUNEL levels, reduction of proliferating cell nuclear antigen and cyclin-dependent kinase 4 expression, and fragmentation of poly (ADP-ribose) polymerase were also elucidated. These results suggest that commercial oral wet wipes could exert cytotoxic influences on oral tissue, although there are anti-bacterial effects, and careful attention is required, especially for infants and toddlers.

• The Journal of Korean Medicine
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• v.31 no.2
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• pp.91-108
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• 2010

Background & Objective: The aim of this study was to investigate the effect of P. radix on the inflammatory related gene expression in IL-$1{\beta}$-stimulated primary human gingival fibroblast using Whole Transcript Sense Target (WT-ST). Method: Human gingival fibroblast was incubated with P. radix [100 or $200\;{\mu}g/ml$], and IL-$1{\beta}$ [$1ng/m{\ell}$] added an hour later. After 24h, total RNA was extracted using RNeasy Mini Kit and the whole gene expression patterns were performed using WT-ST Labeling $Assay^{(R)}$. Result: In the DEG results, 782 genes were up-regulated in the IL-$1{\beta}$-treated group as compared to control and among those, 43 genes were associated with inflammation. 981 genes were down-regulated after treatment with IL-$1{\beta}$ and of those 7 genes were associated with inflammation. 1439 genes were up-regulated after treatment with P. radix plus IL-$1{\beta}$-treated when compared to IL-$1{\beta}$-treated alone group and 1225 genes were down-regulated in the same condition. Among the down-regulated genes, 5 were associated with inflammation- and inhibitor genes such as GDF15 and LIF. In the analysis of the P. radix plus IL-$1{\beta}$-treated group, the most significant pathways were the cytokine-cytokine receptor interaction, toll-like receptor signaling, JAK-STAT signaling and tyrosine metabolism. The gene expression patterns in the P. radix $200{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group appear to be more involved in the metabolism-related pathways than in the $100{\mu}g/m{\ell}$ plus IL-$1{\beta}$-treated group. Conclusion & Discussion: By microarray analysis of gene expression data, we are able to identify gene expression patterns associated with not only anti-inflammation effect but also transcription function of P. radix.