• Tuberculosis and Respiratory Diseases
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• v.72 no.4
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• pp.343-351
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• 2012

Background: The phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling axis has emerged as a novel target for cancer therapy. Agents that inhibit this pathway are currently under development for lung cancer treatment. In the present study, we have tested whether dual inhibition of PI3K/Akt/mTOR signaling can lead to enahnced antitumor effects. We have also examined the role of autophagy during this process. Methods: We analyzed the combination effect of the mTOR inhibitor, temsirolimus, and the Akt inhibitor, GSK690693, on the survival of NCI-H460 and A549 non-small cell lung cancer cells. Cell proliferation was determined by MTT assay and apoptosis induction was evaluated by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. Autophagy induction was also evaluated by acridine orange staining. Changes of apoptosis or autophagy-related proteins were evaluated by western blot analysis. Results: Combination treatment with temsirolimus and GSK690693 caused synergistically increased cell death in NCI-H460 and A549 cells. This was attributable to increased induction of apoptosis. Caspase 3 activation and poly(ADP-ribose) polymerase cleavage accompanied these findings. Autophagy also increased and inhibition of autophagy resulted in increased cell death, suggesting its cytoprotective role during this process. Conclusion: Taken together, our results suggest that the combination of temsirolimus and GSK690693 could be a novel strategy for lung cancer therapy. Inhibition of autophagy could also be a promising method of enhancing the combination effect of these drugs.

• Journal of the Korea Society of Computer and Information
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• v.15 no.2
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• pp.41-46
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• 2010

In this paper, we propose a detection method of reduced computational complexity aimed at separating the moving objects from the background in a generic video sequence. In generally, indoor environments, it is difficult to accurately detect the object because environmental factors, such as lighting changes, shadows, reflections on the floor. First, the background image to detect an object is created. If an object exists in video, on a previously created background images for similarity comparison between the current input image and to detect objects through several operations to generate a mixture image. Mixed-use video and video inputs to detect objects. To complement the objects detected through the labeling process to remove noise components and then apply the technique of morphology complements the object area. Environment variable such as, lighting changes and shadows, to the strength of the object is detected. In this paper, we proposed that environmental factors, such as lighting changes, shadows, reflections on the floor, including the system uses mixture images. Therefore, the existing system more effectively than the object region is detected.

• The Journal of Korean Medicine
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• v.31 no.3
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• pp.55-65
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• 2010

Background: Nitric oxide (NO) is a reactive free radical gas and a messenger molecule. NO has many physiological functions, but excessive NO production induces neurotoxicity. Objective: The present study investigated whether the aqueous extract of Polygala tenuifolia Willdenow possesses a protective effect on NO-induced apoptosis in human neuroblastoma cell line SK-N-MC. Method: For this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and caspase-3 enzyme assay were performed. Result: Sodium nitroprusside (SNP) exposure significantly decreased the viability of cells. The cells treated with SNP exhibited several apoptotic features such as increasing of Bax expression, caspase-3 enzyme activity and inhibiting of Bcl-2 expression. On the other hand, the viability of cells pre-treated with the aqueous extract of Polygala tenuifolia Willdenow was increased dose-dependently. The cells pre-treated for 1 h with the aqueous extract of Polygala tenuifolia Willdenow followed by treatment with SNP showed a decreased occurrence of apoptotic features like decreasing Bax expressions, caspase-3 enzyme activity and increasing Bcl-2 expressions. The aqueous extract of Polygala tenuifolia Willdenow reduced apoptotic cell death in neuroblastoma cell line SK-N-MC through the inhibition of Bax-dependent caspase-3 activation and the increasing of Bcl-2 expression. Conclusion: Based on the present results, it is possible that Polygala tenuifolia Willdenow has therapeutic value for the treatment of a variety of NO-induced brain diseases.

• The Journal of the Korea Contents Association
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• v.6 no.11
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• pp.266-275
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• 2006

Mostly mechanical products are connected by several components instead of single accessory in product process. Although majority of assembly process is automated, the fault analysis is not automated because it needs expert knowledge in various fields to support inclusive decision-marking. This paper proposes an assembly fault analysis support system that uses image regions which can be easily accessed and understood by experts of various fields. An assembly fault analysis support system helps effective fault analysis from assembly by integrating image regions, product design information, and fault detection information. The proposed method enables fault information access from multimedia information by segmenting product images. After product images are segmented by labeling, design information and fault information are integrated in extended Attributed Relational Graph.

• Journal of the Korean Society of Food Science and Nutrition
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• v.14 no.2
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• pp.202-213
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• 1985

The protein nutritional quality of foods has become an important factor to food processors with the advent of nutritional labeling regulations for foods. Then, as is true today, the officially approved assay for protein nutritional quality was the rat based protein efficiency ratio(PER) bioassay. The PER bioassay requires a minimum of 28 days to performe, and is therefore not applicable to routine quality assurance use by the food industry. Within the past ten years there has been a research emphasis placed on the development of rapid, inexpensive, biological and/or chemical based assays for protein nutritional quality. It was hoped that if a rapid assay could be developed and thoroughly tested, it could be used in lieu of the PER bioassay in the day-to-day quality assurance screening of food ingredients and products. The rapid assays developed in the hope of attaining this goal have been based on microorganisms, proteolytic enzymes, and amino acid profiles, as well as combinations of the above. In this review, it will be described and briefly discussed many of procedures which had contributed conceptually as well as practically to the development of in vitro methods for the evaluation of protein quality. Special emphasis will be placed on the C-PER(computed protein efficiency ratio) assay which combines data from in vitro protease digestion and amino acid composition to predict protein nutritional quality designed by Satterlee et al. (1980), and the DC-PER(discriminant computed PER) which is a method of estimating protein quality based on rat assay and in vitro digestibility obtained using solely essential amino acid data will be also introduced.

• Journal of the East Asian Society of Dietary Life
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• v.25 no.2
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• pp.309-315
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• 2015

This study aimed to develop and validate an analytical method for ethyl carbamate (EC) in Maesilju. For accurate analysis of EC in Maesilju, an internal standard (d5-EC) and neutralization (pH=7) were performed. The limit of quantification (LOQ) was 3.65 ppb while the recovery rate ranged from 92.54% to 103.59%. Intra- and inter-day precision ranged from 0.87% to 1.99% and 2.67% to 5.64%, respectively. Maesilju samples comprised 10 homemade and five commercial ones. Samples contained EC at levels between 118.48 and 2,640.42 ppb. The average content of homemade Maesilju was higher than those of commercial ones. Based on manufacturers' labeling of commercial samples and information on homemade samples, various factors such as ingredients and soaking time of Maesil affected EC level in Maesilju. EC contents showed a positive correlation with age of Maesilju.

• Journal of the Korea Society of Computer and Information
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• v.15 no.8
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• pp.31-39
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• 2010

The paper proposes the median filter based Retinex algorithm to detect the lanes in a foggy image. Whether an input image is foggy or not is determined by analyzing the histogram in the pre-defined ROI(Region of Interest). If the image is determined as a foggy one, then it is improved by the median filter based Retinex algorithm. By replacing the Gaussian filter by the median filter in the Retinex algorithm, the processing time can be reduced and the lane features can be detected more robustly. Once the enhanced image is acquired, the binarization based on multi-threshold and the labeling operations are applied. Finally, it detects the lane information using the size and direction parameters of the detected lane features. The proposed algorithm has been evaluated by using various foggy images collected on different road conditions to prove that it detects lanes more robustly in most cases than the conventional methods.

• Journal of the Korean Society of Food Culture
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• v.32 no.6
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• pp.513-526
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• 2017

The purpose of this study is to identify the guideline's contents for dietitians' knowledge, attitudes, and practices (KAP) for the sustainability management at school foodservice. The contents for the guideline were determined by the Delphi technique of two rounds. The Delphi panels of experts were consisted of sixteen school dietitians and fourteen professors of food and nutrition with more than 10 years of experiences by convenience sampling method. Based on the literature, knowledge, attitudes, and practices required for dieticians were classified into menu management, procurement, food production, facility and energy management, waste management, personnel management, and nutrition education. Data were analyzed using SPSS for Windows version 24 and EXCEL to calculate descriptive statistics, content validity ratio, degree of agreement, and degree of convergence. As a result of the second round, the validity scores of 'knows eco-friendly certification standards and labeling systems (4.53 point)' in the knowledge category, and 'tries to purchase local agricultural products (4.87 point)' in the attitude category were the highest. From that round in the practice category, the validity scores of 'plan menus for students' health', 'purchases eco-friendly food', and 'conserves energy in pre-processing and cooking process' were the highest with 4.73 point. Applying the criteria for securing the validity of the contents, the contents of 25 knowledge items, 20 attitude items and 30 practice items were confirmed. The findings of the study can be used to develop the guideline for dietitians required for the sustainability management.

• The Transactions of the Korea Information Processing Society
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• v.7 no.2
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• pp.542-551
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• 2000

In this paper, a novel technique is presented for automatic brain region segmentation in single channel MR image data sets for 3D visualization and analysis. The method detects brain contours in 2D and 3D processing of four steps. The first and the second make a head mask and an initial brain mask by automatic thresholding using a curve fitting technique. The stage 3 reconstructs 3D volume of the initial brain mask by cubic interpolation and generates an intermediate brain mask using morphological operation and labeling of connected components. In the final step, the brain mask is refined by automatic thresholding using curve fitting. This algorithm is useful for fully automatic brain region segmentation of T1-weighted, T2-weighted, PD-weighted, SPGR MRI data sets without considering slice direction and covering a whole volume of a brain. In the experiments, the algorithm was applied to 20 sets of MR images and showed over 0.97 in comparison with manual drawing in similarity index.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.21 no.2
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• pp.73-81
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• 2011

The purpose of this study was to obtain scientific information regarding classification and health hazards that may result from a 13 weeks inhalation exposure of isoprene in Sprague-Dawley (SD) rats. The testing method was conducted in accordance with OECD guidelines for the testing of chemicals No. 413. The Rats were divided into 4 groups (10 male and 10 female rats in each group) and exposed to 0, 360, 1,620, 7,300 ppm isoprene in each exposure chamber for 6 h/day, 5 days/week, for 13 weeks. As a result, there were no mortality or abnormality during the period of study and did not show any significant changes of body weight. There were no dose response changes in urinalysis, hematological and serum biochemical value examination. Relative organ weight was increased significantly the right kidney in 7,300 ppm group of male rats. In female rats, relative organ weight of the left kidney and the both lungs in 1,620 ppm group and the left lung and the both kidneys in 7,300 ppm group were increased significantly. But the histopathological findings did not reveal any exposure-related changes. According to the above results, the no observable adverse effect level (NOAEL) of isoprene was 7,300 ppm (20.3 mg/L) in both male and female rats. In conclusion, Isoprene was not classified specific target organ toxicity of the 'Standard for Classification and Labeling of Chemical Substance and Material Safety Data Sheet' (Ministry of Employment and Labor, 2009).