• Bulletin of the Korean Chemical Society
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• 제34권12호
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• pp.3840-3842
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• 2013

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.672-674
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• 2014

• Natural Product Sciences
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• 제27권4호
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• pp.293-299
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• 2021

The ethyl acetate fractions prepared from the leaves of Thuja orientalis significantly inhibited nitric oxide (NO) production in lipopolysaccharide-stimulated BV2 microglial cells. According to bioassay-coupled LC-QTOF MS/MS, the components near 22 and 25 mins in the mass chromatogram highly inhibited NO production and were expected to be labdane diterpenes, and the active components were characterized via further isolation. The results of the NO production inhibitory assay of the isolated compounds correlated well with the results of bioassay-coupled LC-QTOF MS/MS. Among the identified constituents, NO production inhibitory activities of 16-hydroxy-labda-8(17),13-diene-15,19-dioic acid butenolide (2) and 15-hydroxypinusolidic acid (3) were newly reported. Taken together, these results demonstrated that LC-QTOF MS/MS coupled with NO production inhibition assay was a powerful tool for accurately predicting new anti-inflammatory constituents in the extracts from natural products. Moreover, it provided a potential basis for broadening the application of bioassay-coupled LC-QTOF MS/MS in natural product research.

• 대한화학회지
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• 제59권2호
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• pp.179-182
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• 2015

• Journal of the Korean Wood Science and Technology
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• 제26권4호
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• pp.71-77
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• 1998

To elucidate chemical structure of the wood extractive, wood meal of Larix leptolepis Gorden was extracted with 95% ethanol for 72 hours. The extract was fractionated with organic solvents such as n-hexane, chloroform, diethylether, and ethyl acetate. From the hexane somble fraction of the extractives, a new diterpene compound was isolated and identified as 6-acetoxymanool or 13-hydroxy-8(17), 14-labddienyl-6-acetate by IR, $^1H(^{13}C)$-NMR and Mass spectrometry.

• Applied Biological Chemistry
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• 제46권3호
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• pp.246-250
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• 2003

양하(Zingiber mioga Roscoe) 근경의 에탄을 추출물을 재료로 Staphylococcus aureus, Bacillus subtilis 및 B. cereus에 대한 activity-guided fractionation을 실시하여 항균성 물질 세가지준 얻었다. 항균력실험 결과, 화합물 I과 111은 세가지균주 모두에 활성이 있었고, 화합물 II에서는 B. subtilis과 B. cereus에서만 활성이 나타났다. 그 중에서 가장 활성이 강한 화합물 I을 Bioscreen C로 optical density(600 nm)를 측정하여 증식억제실험을 한 결과 10 ppm 처리시 B. subtilis과 B. cereus에서 72시간동안 강한 증식억제효과를 나타내었으며, S. aureus에서는 25 ppm 처리시 72시간동안 완전증식억제효과를 나타내었다. $1^H-NMR$, ${13}^C-NMR$, DEPT,$1^H-1^H$ COSY, HMQC, HMBC 및 IR 스펙트럼 등을 분석한 결과 화합물 I, II, III은 labdane-type diterpene인 $(E)-8{\beta}(17)-epoxylabd-12-ene-15,16-dial(C_{20}H_{30}O_3,\;MW=318)$, $galanolactone(C_{20}H_{30}O_3,\;MW=318)$ 그리고 galanal A($C_{20}H_{30}O_3,\;MW=318$)로 각각 동정되었으며, 이들은 양하의 근경에서는 처음 분리된 것이다.

• Plant Resources
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• 제4권2호
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• pp.65-74
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• 2001

Coleus forskohlii Briq, of the family Lamiaceae yields a valuable secondary metabolity known as forskolin which is a labdane diterpenoid.. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root rot disease. The fungal culture filterate (ECE) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% ECF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forshohlii. This data could prove to be useful for the future for selecting a resistant C.forskohlii plant against the root disease caused by L. theobromae.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.96-113
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• 2000

Coleus forskohlii Briq. of the family Lamiaceae yields a valuable secondary metabolite known as forskolin which is a labdane diterpenoid. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root of disease. The fungal culture filterate (FCF) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% FCF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forskohlii. This data could prove to be useful for the future for selecting a resistant C,forskohlii plant against the root disease caused by L.theobromae.