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Peptide Properties of Rapid Salted and Fermented Anchovy Sauce Using Various Pretenses 2. Characterization of Hydrolytic Peptides from Anchovy Sauce and Actomyosin (단백질 분해효소를 이용하여 제조한 속성 멸치 액젓의 펩티드 특성 2. 멸치 액젓 및 Actomyosin의 가수분해 펩티드의 특성)

  • CHOI Yeung-Joon;KIM In-Soo;CHO Young-Je;SEO Duck-Hoon;LEE Tae-Gee;PARK Yeung-Beom;PARK Jae-Woon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.4
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    • pp.488-494
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    • 1999
  • Hydrolytic peptides of salted and fermented anchovy sauce, and anchovy actomyosin for the development of a rapid fermentation method with conventional tastes and flavors were studied. The optimal temperatures of crude enzymes isolated from anchor, liver and viscera of squid were 55, 40$\~$45 and $45\~60^{\circ}C$, respectively. Crude enzyme isolated from anchovy was more effective on hydrolysis of anchovy actomyosin than that from squid liver and viscera. But the crude enzyme from squid liver was less effective on NaCl than that from anchovy. Three peptides occurred in anchovy actomyosin hydrolyzed with crude enzymes from anchovy and squid liver for 30 min. Their molecular weight were determined by Superdex 200 gel chromatography as 10,800, 5,800 and 2,600 dalton. When anchovy sauce was hydrolyzed with crude enzymes of anchovy, squid liver and viscera, and Protamex during 70 days, ranges of their low molecular weight of hydrolyzed peptides were 300$\~$1,000dalton detected by Sephadex G-50 and their major amino acid compositions were glutamic acid, glycine and alanine, which was related with conventional tastes. Those amino acid compositions were similar to those of anchovy sauce hydrolyzed with squid liver, In the case of Protamex treatment, hydrolyzed peptides had high levels of isoleucine and leucine, being associated with the bitter, but a low level of glutamic acid.

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Effect of Antler Velvet Ethanol Extract on Common Serum Chemistry Panels and Histopathological Change in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin (녹용 에탄올 추출물이 2,3,7,8 Tetrachlorodibenzo-p-dioxin에 노출된 랫드의 일반 혈액 화학 지수 및 조직 병변에 미치는 효과)

  • Choi, Kyung-Yun;Hwang, Seock-Yeon;Lee, Su-Chan;Kim, Si-Kwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1178-1184
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    • 2006
  • This study was carried out to investigate the effect of ethanol extract of antler velvet (EAV) on common serum chemistry panels and histopathological change in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Administration of TCDD ($50{\mu}g/kg$ body weight) induced significant decrease in platelet count (p<0.01), creatine phosphokinase (CPK, p<0.01), lactatate dehydrogenase (LDH, p<0.05) and glucose (p<0.05) levels and increase in hemoglobin (p<0.05), aspartate aminotransferase (AST, p<0.01), alanine amino transferase (ALT, p<0.05) and lipase activities (p<0.05), and blood urea nitrogen (BUN, p<0.05), triglyceride (p<0.01) and low density lipoptotein cholesterol (LDL-C, p<0.05) levels. However, pretreatment of EAV at daily dose of 20 mg/kg b.w. from 1 wk before TCDD exposure for 5 wks attenuated the abnormality of the overall serum chemistry panels but statistical difference between TE and TA groups was observed only in testicular weight (p<0.01), LDH activity (p<0.05), glucose (p<0.05) and lipase activity (p<0.01). In addition, TCDD induced significant histopathological changes including swelling, fatty metamorphosis, and vacuolar degeneration in liver; edema in proximal and distal convoluted tubules, and glomerulus in kidney; severe atrophy of red purple and appearance of significant number of macrophage in spleen; prominent atrophy and decrease in immune cells in thymus. On the other hand, administration of EAV attenuated histopathological damage induced by TCDD. These results further suggest that administration of EAV attenuates TCDD induced testicular, liver, pancreatic, hematopoietic and nephrotic toxicities in rats.

A Study on the Health Status of Dental Technicians by Todai Health Index(THI) (THI에 의한 일부 치과기공사의 건강상태에 관한 조사 연구)

  • Kwon, Soon-Suk;Moon, Hee-Jung;Shin, Myung-Suk;Kim, Yoon-Shin
    • Journal of dental hygiene science
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    • v.9 no.2
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    • pp.169-179
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    • 2009
  • This study aims to present a fundamental data base to figure out the mental and the physical conditions that the dental technicians are facing and ultimately to develop a health care program to deal with their health related problems. To this end, we took an analysis on the health status among the subjects of 895 dental technicians currently working at the dental lab around the nation from January 15 to March 31, 2009 by way or Todai Health Index(THI). Of the average scale point in accordance with 12 scale scores of the physical and the mental subjective symptom, the results revealed that the physical appeals (21.10) were higher than the mental appeals (18.49) and the multiple subjective symptom was marked as 38.44 followed by the mental irritability (25.92). In gender differences, the females proved to be higher than the males in both physical appeals and mental appeals while the physical appeals were dominant in both genders. The physical appeals were higher than the mental appeals with regard to the general characteristics. In the case or the group or age twenties as shown in the physical and mental average scale point, the other groups showed 21.55% of the physical appeals among the married whereas the mental appeals showed the highest point as 18.70 in the unmarried group. In job position, the other groups marked the highest, in working condition, below average group marked the highest, in frequency of break time, none group marked the highest. We drew a conclusion form this study that the dental technicians gained the higher points in the item or the multiple subjective symptom, the menial irritability, and the irregular life. More research on th is phenomena should be followed along with the development of various and practical health care programs to promote the health or dental technicians.

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The Effect of Perfectionism on Stress and Anxiety during Scaling Practice (완벽주의가 스케일링 실습 시 실습불안과 스트레스에 미치는 영향)

  • Lim, Soon-Ryun;Woo, Hee-Sun
    • Journal of dental hygiene science
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    • v.9 no.2
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    • pp.161-167
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    • 2009
  • The purpose of this Study was to examine the effect of perfectionism on stress and anxiety during scaling practice in an effort to find efficient way of helping students with getting good skills. The subjects in this study were students who practiced a scaling at the oral hygiene practice lab in the Department of dental hygiene in S college from May 1 to May 31, 2008. They were divided into four groups based on their subscales of perfectionism : mixed perfectionist group, achievement striving perfectionist group, failure avoidance perfectionist group and non-perfectionist group. The measurements used were Two-Dimensional Perfectionism Scale, Stress level, Trait anxiety, State anxiety. There were no significant differences in the stress level before practice between 4 groups. There were significant differences in trait anxiety, state anxiety, total anxiety before scaling practice between 4 groups. However, these results were due to differences between mixed perfectionist group and non-perfectionist group. After practice, total anxiety was decreased from 93.71 to 89.66 and state anxiety was decreased from 45.49 to 43.38. These results were statistically significant. In order to investigate the influence of achievement striving factor and failure avoidance factor on the change of state anxiety during the scaling practice Standard Multiple Regression were employed for the statistical analysis. Failure avoidance factor was related with the increase of state anxiety during the scaling practice. So leachers have to give all effort to reduce the anxiety of students during scaling practice and provide students with motivation of achievement.

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The Changes of Smart Device Usage Status in Early Childhood: Comparison of 2015-2016 and 2017 Studies (유아 스마트 기기 사용 현황의 변화: 2015-2016년과 2017년의 연구 결과 비교)

  • So, Hyejin;Lim, Sungmin;Cho, Sang Yeun;Koh, Min Suk;Moon, Jin-Hwa
    • Journal of the Korean Child Neurology Society
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    • v.26 no.4
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    • pp.251-262
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    • 2018
  • Purpose: This study aimed to identify changes in smart device usage trends of young children using two studies conducted in 2015-2016 and 2017 respectively. Methods: We compared the data of the previous study of 130 children (Group A) and the new study of 162 children (Group B). The children and parents were recruited from kindergartens in Seoul and Guri/Namyangju cities. We used the "Parental questionnaire for smart device usage status." Results: There were some changes in the smart device usage in young children and parental perception. In the 2017 study, smart device usage time increased during weekends (P<0.05) and the usage with siblings decreased (P<0.05). In 2017, the smart device was mostly used when children had to be quiet without disturbing others (36.8%). No significant difference existed in the main purpose of use: watching video clips (79.3% vs 76.6%). Overall control of the usage was still largely exercised by mothers; however, when using applications, mothers still only helped the children on request (51.8% vs 49.7%). Regarding the effect of smart device on children, responses of "not knowing" decreased and "will be negative" and "will be positive" increased (P<0.05). Additionally, most mothers thought that "Although the smart device is currently unnecessary, it will be needed in future" in 2017 (46.3%). Conclusion: Limiting the smart device usage time during the weekends and increasing parental involvements are recommended. Guidelines for smart devices usage in young children are also necessary considering the changes in parental attitudes in recognizing the smart device usage as unavoidable.

Effect of Temperature and Water Content of Soil on Creeping Bentgrass(Agrostis palustris Huds) Growth (토양의 온도와 수분이 크리핑 벤트그래스(Agrostis palustris Huds) 생육에 미치는 영향)

  • Lim, Seung-Hyun;Jeong, Jun-Ki;Kim, Ki-Dong;Joo, Young-Kyoo
    • Asian Journal of Turfgrass Science
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    • v.23 no.2
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    • pp.229-240
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    • 2009
  • The high temperature and water content in soil profile probably affect the physiological disorder especially on cool-season turfgrasses in warm and humid weather of Korean summer. The purpose of this research was to analyze the effect of soil temperature and water content on the growth and stress response of creeping bentgrass(Agrostis palustris Huds.) under a humid and warm temperature. USGA(United State of Golf Association) green profile in laboratory test, Daily temperature changes were tested under a dried sand, 70% water content of field capacity, and saturated condition at $34^{\circ}C$ of the USGA green in lab. In this test, the dried sand reached to $80^{\circ}C$, however, the surface temperature decrease of $10^{\circ}C$ on the saturated condition. In the thermal properties test in field, thermal conductivity, thermal diffusivity, and soil temperature were increased followed by irrigation practise. In the water-deficient condition, the highest soil temperature was reached temporally right after irrigation, however, the excessive soil water content higher than field water holding capacity showed the highest soil temperature after a while. This result indicated that a heat damage to root system was caused from the thermal conductivity of a high surface soil temperature. The excessive irrigation when a high turf surface temperature should occur a negative result on tufgrass growth, moreover, it would be fatal to root growth of creeping bentgrass, especially when associated with a poor draining system on USGA sand green. Overall, this study shows that high soil temperature with water-excessive condition negatively affects on cool-season grass during the summer season, suggesting that excessive irrigation, over 70% field capacity of soil condition, does not help to reduce soil temperature for summer season in Korea. In the study that cool-season grass were treated with different water content of soil, The soil had higher temperature and more water holding capacity when treatment rate of soil conditioner was increased. The best growth at the normal water condition and the worst state of growth at thee water-excessive condition were observed.

A Novel Volumetric Method for Quantitation of Titanium Dioxide in Cosmetics (용량분석법을 이용한 화장품 중 티타늄옥사이드의 정량)

  • Kim, Young-So;Kim, Boo-Min;Park, Sang-Chul;Jeong, Hye-Jin;Chang, Ih-Seop
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.4 s.54
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    • pp.289-293
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    • 2005
  • Nowadays there are many sun protection cosmetics including organic or inorganic UV filter as an active ingredient. Chemically stable inorganic sunsEreen agents, usually metal oxides, we widely employed in high SPF products. Titanium dioxide is one of the most frequently used inorganic UV filters. It has been used as pigments for a long period of cosmetic history. With the development of micronization techniques, it becomes possible to incorporate titanium dioxide in sunscreen formulations without whitening effect and it becomes an important research topic. However, there are very few works related to quantitations of titanium dioxide in sunscreen products. In this research, we analyzed amounts of titanium dioxide in sunscreen cosmetics by adapting redof titration, reduction of Ti(IV) to Ti(III) and reoxidation to Ti(IV). After calcification of other organic ingredients of cosmetics, titanium dioxide is dissolved by hot sulfuric acid. The dissolved Ti(IV) is reduced to the Ti(III) by adding aluminum metals. The reduced Ti(III) is titrated against a standard oxidizing agent, Fe(III) (ammonium iron(III) sulfate), with potassium thiocyanate as an indicator In order to test accuracy and applicability of the proposed method, we analyzed the amounts of titanium dioxide in four types of sunscreen cosmetics, such as cream, make-up base, foundation and powder, after adding known amounts of titanium dioxide $(1{\sim}25w/w%)$. The percent recoveries of the titanium dioxide in four types of formulations were in the range between 96 and 105%. We also analyzed 7 commercial cosmetic products labeled titanium dioxide as an ingredient and compared the results with those of obtained from ICP-AES (Inductively Coupled Plasma-Atomic Emission Spectrometry), one of the most powerful atomic analysis techniques. The results showed that the titrated amounts were well coincided with the analyzed amounts of titanium dioxide by ICP-AES. Although instrumental analytical methods, ICP-MS (Inductively Coupled Plasma-Mass Spectrometry) and ICP-AES, are the best for the analysis of titanium, it is hard to adopt because of their high prices for small cosmetic companies. It was found that the volumetric method presented here gat e quantitative and reliable results with routine lab-wares and chemicals.

An Investigation of Treatment Effects of Limestone and Steel Refining Slag for Stabilization of Arsenic and Heavy Metal in the Farmland Soils nearby Abandoned Metal Mine (폐금속 광산 주변 비소 및 중금속 오염농경지의 안정화 처리를 위한 석회석과 제강슬래그의 처리효과 검토)

  • Yun, Sung-Wook;Kang, Sin-Il;Jin, Hae-Geun;Kim, Ha-Jin;Lim, Young-Cheol;Yi, Ji-Min;Yu, Chan
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.734-744
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    • 2011
  • A soil stabilization method is an effective and practical remediation alternative for arsenic (As) and heavy metal contaminated farmland soils nearby abandoned metal mine in Korea. This method is a technique whereby amendments are incorporated and mixed with a contaminated soil. Toxic metal bind to the amendments, which reduce their mobility in soil, so the successful stabilization of multi-element contaminated soil depends on the combination of critical elements in the soil and the type of amendments. The objective of this study is to investigate the treatment effects and applicability of limestone (LS) and steel refining slag (SRS) as the amendment for farmland soil contaminated with As and heavy metals, and a lab-column test was conducted for achieving this purpose. The result showed that soil treated with LS and SRS maintained pH buffer capacity and, as a result, the heavy metal leaching concentration was quite low below the water quality standard compared to untreated soil which leachate exceeding the water quality standard was observed, however, the arsenic concentration rather increased with increasing mixture ratio of SRS. This was believed to be related to phosphorus (P) contained in SRS, and dominancy in the competitive adsorption relation between As and P binding strongly to iron might be different according to soil characteristic. We suggested that LS is a effective amendment for reducing heavy metals in soil, and SRS should be used after investigating its applicability based on the adsorption selectivity of arsenic and phosphorus in selected soil.

Studies on Ecological Environments and Indoor-rearing Conditions of the Common Grass Yellow Butterfly, Eurema hecabe (남방노랑나비(Eurema hecabe)의 생태환경 및 실내사육 조건에 관한 연구)

  • Lee, Sang-Hyun;Kim, Se-Gwon;Nam, Gyoung-Pil;Son, Jai-Duk;Lee, Jin-Gu;Park, Young-Kyu;Choi, Young-Cheol;Lee, Young-Bo
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.133-139
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    • 2012
  • The Aims of this study are to investigate ecological environments and indoor-rearing conditions of Eurema hecabe for artificial rearing. Two different nesting site, a open area nearby small river(A area; Daejin-ri, Gonyang-myeon, Sachun-si, Gyoungsangnam-do) and a foot of a hill area(B area; Noryang-ri, Seolcheon-myeon, Namhae-gun, Kyoungsangnam-do), selected for investigation of ecological habitat environment, and investigated two times per month, during six month, from June to November in 2010. In result, the number of butterflies, Eurema hecabe, at the hill area(B area) was 6 times more than the open area(A area). We observed the number of eggs, larvae and puapae in the B area more times compared to those in the A area. In the B area, Lespedeza cuneata was higher preference than the other host plant, Lespedeza pilosa and Albizia julibrissin. For development of indoor-rearing conditions, common grass yellow butterfly, Eurema hecabe, was reared in a room condition. As a result of oviposition preference experiment, 100-mated-female laid significantly higher number of eggs $104.9{\pm}19.6$ on Lespedeza cuneata plant, whereas the number of eggs was $12.7{\pm}4.5$ on Cassia obtusifolia plant. Moreover, the eggs hatched within $5.1{\pm}0.9$ days from the day of oviposition and procent of egg hatchability was 53.7% on host plant. The headwidth of each developmental larval stage were $0.36{\pm}0.02$(1st), $0.61{\pm}0.02$(2nd), $0.93{\pm}0.05$(3rd), $1.46{\pm}0.08$(4th), $2.25{\pm}0.11$(5th). The larval period was $12.1{\pm}0.9$ days under high temperature, long day condition($25^{\circ}C$, 16L : 8D), showing 81.0% pupal ratio. The pupal period was $6.9{\pm}0.7$ days, and the emergence rate was 79.6%. Based on above experiment, artificial rearing system of common grass yellow butterfly, Eurema hecabe was completed in indoor condition.

Analysis of Building Characteristics and Temporal Changes of Fire Alarms (건물 특성과 시간적 변화가 소방시설관리시스템의 화재알람에 미치는 영향 분석 연구)

  • Lim, Gwanmuk;Ko, Seoltae;Kim, Yoosin;Park, Keon Chul
    • Journal of Internet Computing and Services
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    • v.22 no.4
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    • pp.83-98
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    • 2021
  • The purpose of this study to find the factors influencing the fire alarms using IoT firefighting facility management system data of Seoul Fire & Disaster Headquarters, and to present academic implications for establishing an effective prevention system of fire situation. As the number of high and complex buildings increases and former bulidings are advanced, the fire detection facilities that can quickly respond to emergency situations are also increasing. However, if the accuracy of the fire situation is incorrectly detected and the accuracy is lowered, the inconvenience of the residents increases and the reliability decreases. Therefore, it is necessary to improve accuracy of the system through efficient inspection and the internal environment investigation of buildings. The purpose of this study is to find out that false detection may occur due to building characteristics such as usage or time, and to aim of emphasizing the need for efficient system inspection and controlling the internal environment. As a result, it is found that the size(total area) of the building had the greatest effect on the fire alarms, and the fire alarms increased as private buildings, R-type receivers, and a large number of failure or shutoff days. In addition, factors that influencing fire alarms were different depending on the main usage of the building. In terms of time, it was found to follow people's daily patterns during weekdays(9 am to 6 pm), and each peaked around 10 am and 2 pm. This study was claimed that it is necessary to investigate the building environment that caused the fire alarms, along with the system internal inspection. Also, it propose additional recording of building environment data in real-time for follow-up research and system enhancement.