• Applied Biological Chemistry
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• v.38 no.3
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• pp.191-195
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• 1995

Production of a high viscosity exoploysaccharide, methylan, by Methylobacterium organophilum from methanol was carried out in fed-batch cultures and the rheological properties of methylan fermentation broth were studied. Bacterial biomass showed little influence on viscosity, but the accumulation of methylan caused the increase of viscosity. With proceeding fermention, the viscosity at the same concentration of methylan was significantly increased and methylan solution showed slightly higher pseudoplasticity. The composition changes of methylan were investigated at various fermentation times. Contents of total sugar, reducing sugar and methylan were decreased but contents of acids(pyruvic acid, uronic acid and acetic acid) were increased with the culture time. It was considered that the increased content of acids resulted in the increase of the hyrodynamic domain in the solution due to charge repulsion. Consequently, the solution viscosity increased in propotion to the acids contents of methylan. Cell growth and methylan production were severely decreased by the limitation of dissolved oxygen. However, the cellular activity for methylan production was almost constant regardless of the level of dissolved oxygen. As a result, the high speed of agitation increased the methylan production, the specific production rate of methylan, and the methylan yield of the cell.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.190-195
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• 2000

Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease forming brown, slightly sunken spots and blotches on the cultivated mushrooms. It is a lipodepsipeptide consisting of 18 amino acids and its molecular mass is 1,985 Da. It forms a pore in plasma membranes, resulting in the disruption of membranes of fungal, bacterial, plant, and animal cells as well as mushroom tissue. In order to measure the toxicity of tolaasin, erythrocytes of blood were used to evaluate the tolaasin-induced hemolysis. Hemolytic activity of tolaasin was measured by observing the absorbance change either at 420 nm, representing the release of hemoglobins from red blood cells(RBCs), or at 600 nm, representing the density of residual cells. The hemolytic activity of culture-extract of P. tolaasii increased at early-stationary phase of growth and was maximal at late stationary phase. The hemolytic activity of tolaasin appeared high in the RBCs of dog and rat. The RBCs of rabbit and hen were less susceptible to tolaasin. The effects of various cations were also measured. $Cd^{2+}$ and $La^{3+}$. as well as $Zn^{2+}$ appeared inhibitory to the tolaasin-induced hemolysis. The effects of various anions on tolaasin-induced hemolysis were measured and carbonate showed the greatest inhibition to the hemolysis. However, phosphate stimulated the tolaasin-induced hemolysis and no effects were observed by chloride and nitrate.

• The KIPS Transactions:PartD
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• v.18D no.4
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• pp.245-260
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• 2011

Mobile Devices, which are developed with the fast growing of the Internet, have flexible internet accessibility and can access the network anywere so that they can execute software applications. However, it is very challenging to deploy highly complex applications on mobile devices since they have limited resources. To overcome the limitation, researches on applying a concept of services to mobile applications have been proposed. Android is one of the popular mobile platforms and is designed to effectively execute mobile applications on the mobile devices having limited resources. Since service-based Android applications, which adopt a concept of services and Android platform, invoke remote services and are built with Android-specific components, they are much different from traditional software applications. Consequently, it is not straightforward to apply object-oriented (OO) analysis and design methods to developing service-based Android applications, although they have been frequently applied to developing traditional applications. In this paper, we present a process to develop service-based Android mobile applications, which extends a traditional OO development process. First, we raise design issues to be considered in developing service-based Android applications. Then, to solve the issues, we present detailed guidelines for essential phases of OO-based development process that are customized to service-based Android applications. Finally, to show applicability of the process, we perform a case study. The proposed design process is effectively utilized as a set of guidelines to develop service-based Android applications more systematically and effectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1126-1139
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• 2014

World is experiencing abnormal weather caused by urbanization and industrialization increasing greenhouse gas and one of these phenomenon domestically happening is flood and drought. The increase of green-house gases is due to urbanization and industrialization acceleration which are causing abnormal climate changes such as the El Nino and a La Nina phenomenon. It is expected that there will be many difficulties in water management, especially considering the topography and seasonal circumstances in Korea. Unlike in the past, a variety of water conservation initiatives have been undertaken like the river-management flow and water capacity expansion projects. To meet the increasing demand for water resources, new environmentally-friendly small and medium-sized dams have been built. Therefore, the development of a new paradigm for water resources management is essential. This study shows that additional security is needed for potential water resources through diversion tunnels and is very important to consider for future water supplies and situations. Using RCP 6.0 and RCP 8.5 in representative concentration pathway climate change scenario, specific hydrologic data of study basin was produced to analyze past observed basin rainfall tendency which showed both scenario 5%~9% range increase in rainfall. Through sensitivity analysis using objective function, population in highest goodness was 1000 and cross rate was 80%. In conclusion, it is expected that the results from this study will help to make long-term and stable water supply plans by using the potential water resource evaluation model which was applied in this study.

• Journal of Soil and Groundwater Environment
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• v.10 no.4
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• pp.26-32
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• 2005

Surfactants can be used to enhance the mass transfer rate of hydrophobic compounds into the biologically active liquid phase, resulting in an increase in biodegradation rate of toluene. In this study, the mass transfer rate and the biocompatibility of toluene in the presence of various surfactants were evaluated. Four anionic and non ionic surfactants were tested: sodium dodecyl sulfate (SOS), TritonX-100, Tween 80, and BYK-345 (silicone surfactant). Experimental results showed that BYK-345 at the critical micelle concentration (CMC) enhanced the solubility of toluene. However, there was no increase in the solubility of toluene by SOS and TritonX-100 at their CMCs. With the addition of each surfactant into deionized water the mass transfer rate became faster than that of the case with no surfactant. A bottle study using toluene-degrading microorganisms showed that SOS seriously reduced toluene removal presumably due to the toxicity of the anionic surfactant and/or the substrate competition between the surfactant and toluene. In addition, the degradation rate of toluene was decreased in the presence of BYK-345, indicating that BYK-345 adversely affects the activity of microorganisms. However, TritonX-100 and Tween 80 did not decrease the degradation rate of toluene significantly. Rather, at the low concentration of TritonX-100 toluene degradation rate was even increased. Overall the experimental results suggest that TritonX-100 be the appropriate surfactant for enhanced biological degradation of toluene.

• Polymer(Korea)
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• v.29 no.5
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• pp.468-474
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• 2005

PLGA and PCL copolymers initiated by carbitol as drug carriers were synthesized by ring-opening polymerization of L-lactide (LA), glycolide (GA), and $\varepsilon-caprolactone(\varepsilon-CL)$. Implantable wafers were simply fabricated by direct compression method after physical mixing of copolymers and bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) as a model protein drug. The release amounts of BSA-FITC from wafers were determined by fluorescence intensity using the fluorescence spectrophotometer. Also, the release behavior of BSA-FITC on wafers was controlled by adding the additives such as collagen, small intestinal submucosa (SIS), poly(vinyl pyrrolidone) (PVP), and poly(thylene glycol) (PEG). The wafer prepared by PLGA and PCL exhibited slow release within $10\%$ for 30 days. But, those prepared by a variety of additives exhibited the controlled BSA release patterns with a dependence on the additive contents. furthermore, the wafers containing natural materials such as collagen and SIS showed more zero-order release profile than that with synthetic materials such as PVP and PEG. It was confirmed that the release of BSA from implantable wafers could be easily controlled by adding natural additives.

• Journal of Korean Society of Transportation
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• v.19 no.6
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• pp.207-218
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• 2001

As a city and industries are developed rapidly, a traffic accident and congestion take places on the road link become serious and it can be a large problem of the society in the future. Especially, most of the traffic accidents on the signalized intersection are caused by the human factor, vehicle and environmental factor mutually. The relation of the traffic accident and volume is acting on the outbreak of the traffic accident and the mistake of driver altogether as a major cause. The purpose of this paper is to develop a model for the forecasting of the traffic accident and to use research data gained to predict many traffic accidents. The data of this study were used with real one of the 73 areas of the four-legged signalized intersection in Kwang-ju city from 1996 to 1998 for three years to develop a model for the forecasting of the traffic accident. The statistical methods used in this paper are the principal component, regression and correlation analysis. We studied accident models to find out useful data from the statistics method and applied the data to the different area of the Choun-La province for the verification of the model. So, the result of this paper showed a reasonable model for the forecasting or the traffic accident and possibility of the model for simulating on real case. Finally, This study would be made of a study continually for the safe design and plan for the four-legged signalized intersection.

• Journal of Wetlands Research
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• v.20 no.1
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• pp.43-53
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• 2018

Laguna Lake is the largest inland fresh water body in the Philippines. It primarily serves as a site for aquaculture, hydropower, transportation, and water supply industries. Due to Laguna Lake's diverse functionalities, competition among water users became prominent. Water quality began to deteriorate due to various pollutant contributions in this process, thereby affecting the soundness of the aquatic ecosystem. This study was conducted to evaluate the current water quality management policy from the viewpoint of ecological environment through the evaluation of the water quality of Laguna Lake. Concentrations of water pollutants such as ammonia ($NH_3$), biochemical oxygen demand (BOD), chloride ($Cl^-$), pH, and total suspended solids (TSS) exceeded the water quality standards of the Philippines' Department of Environment and Natural Resources (DENR). The water quality of the lake was also affected by the pollutant load due to agriculture and urban stormwater runoff in the watershed. The salinity and contaminated water from Pasig River also affected the water quality of Laguna Lake. Long-term water quality analysis showed that the water quality of Laguna Lake is also influenced by rainfall-related seasonal variations. The results of the water quality analysis of Laguna Lake indicated that the environmental management techniques of the Philippines should be changed from the conventional water management into an integrated watershed management scheme in the future. It is therefore necessary to study and introduce advanced watershed management measures in the Philippines based from the policies of other developed countries.

• Journal of Veterinary Clinics
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• v.30 no.2
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• pp.87-94
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• 2013

This retrospective study reviewed the clinical and diagnostic features, therapeutic outcome and compilations of interventional ductal occlusion in 37 dogs with patent ductus arteriosus (PDA). Malteses and female dogs were over-presented. Bounding pulse and left basal continuous murmur were most common findings in physical examination, while the differential cyanosis was rarely observed. Left ventricular (LV) enlargement patterns and sinus tachycardia were common in the ECG. Typical radiographic findings included LV elongation and triple bumps indicating left atrial (LA), aortic and pulmonary dilation. Echocardiographic features were typical shunt flow at the ductus arteriosus and marked LV dilation with mild to moderate mitral regurgitation (MR). The 32 of 37 dogs were interventionally treated with either thromboemolic coils (TCE) or Amplatz canine ductal occluder (ACDO). Transient hemoglobinuria caused by incomplete closure was occurred in 2 dogs treated with TCE, although the hemoglobinuria was disappeared within a week of intervention. The dislodgement of occlusion device was occurred in 2 dogs with TCE and 1 dog with ACDO. However there were no significant complications associated with this dislodgement, since those were dislodged at the lower pulmonary vasculature. We also found that no serious complications and no further medical intervention in 29 dogs having long-term follow-ups.

• Journal of Korean Society on Water Environment
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• v.23 no.2
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• pp.201-205
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• 2007

This paper reports the amount of $^{222}Rn$ and $^{238}U$ in 18 sites of ground water and 30 sites of surface water. The instrument used to count $^{222}Rn$ activity was the liquid scintillation counter (LSC) which could resolute ${\alpha}$ and ${\beta}$ radiations. And $^{238}U$ was analyzed by the inductively coupled plasma (ICP). Radon and Uranium were not detected in raw and treated water which were sampled in a water treatment plant. However, radon ($^{222}Rn$) was high concentration in ground water from Jeon-la, Gang-won. So was uranium ($^{238}U$) in case of ground water from Gang-won, Choong-chung. Radon ($^{222}Rn$) activities were detected less than 15 pCi/L at 5 sampling points, 15~300 pCi/L at 7 sampling points, 300~4000 pCi/L at 6 sampling points. However, Radon ($^{222}Rn$) activities of all ground water samples were less than 4,000 pCi/L, which was bellow American Alternative Maximum Contamination Level (AMCL). Uranium ($^{238}U$) concentrations were less than $0.1{\mu}g/L$ at 5 sampling points, from $0.1{\mu}g/L$ to $20{\mu}g/L$ at 13 sampling points. Uranium was not detected in about 30% of the whole samples, but the concentration ranged from relatively low to high concentrations depending on the sampling point. The minimum detectable activity (MDA) of radon was 15 pCi/L. and the detection limit of uranium was $0.1{\mu}g/L$.