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검색결과 442건 처리시간 0.026초

Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)

  • Li Muwang;Li Minghui;Miao Xuexia;Lu Cheng;Huang Yongping
    • International Journal of Industrial Entomology and Biomaterials
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    • 제11권1호
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    • pp.49-55
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    • 2005
  • Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.

MultiView-Based Hand Posture Recognition Method Based on Point Cloud

  • Xu, Wenkai;Lee, Ick-Soo;Lee, Suk-Kwan;Lu, Bo;Lee, Eung-Joo
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • 제9권7호
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    • pp.2585-2598
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    • 2015
  • Hand posture recognition has played a very important role in Human Computer Interaction (HCI) and Computer Vision (CV) for many years. The challenge arises mainly due to self-occlusions caused by the limited view of the camera. In this paper, a robust hand posture recognition approach based on 3D point cloud from two RGB-D sensors (Kinect) is proposed to make maximum use of 3D information from depth map. Through noise reduction and registering two point sets obtained satisfactory from two views as we designed, a multi-viewed hand posture point cloud with most 3D information can be acquired. Moreover, we utilize the accurate reconstruction and classify each point cloud by directly matching the normalized point set with the templates of different classes from dataset, which can reduce the training time and calculation. Experimental results based on posture dataset captured by Kinect sensors (from digit 1 to 10) demonstrate the effectiveness of the proposed method.

Postmortem Proteolysis of Breast and Leg Muscles from Taiwan Colored Chickens and Silkie Bantams

  • Tsai, Shih-Fen;Lin, Chia-Ying;Lu, Jin-Jenn;Chou, Rong-Ghi R.
    • Asian-Australasian Journal of Animal Sciences
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    • 제19권5호
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    • pp.739-743
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    • 2006
  • Postmortem proteolysis of breast (BM) and leg (LM) muscles from Taiwan colored chickens (TCC) and silkie bantams (SB) at $5^{\circ}C$ were compared. Myofibrils were prepared from BM and LM samples that were randomly taken from the carcasses of SB and TCC after 0, 1, 3, 7 and 14 days of storage at $5^{\circ}C$. pH of samples was determined, and degradation of myofibrillar proteins was analyzed by the SDS-PAGE and western blots. The results showed that pH was lower in BM than in LM samples from both avian strains. Appearance of 30 kDa components and disappearance of titin and nebulin were more rapidly as seen on SDS-PAGE in BM than in LM samples. Western blots labeled with a monoclonal antibody to desmin also demonstrated that desmin degraded more quickly in BM samples. Our data might suggest that postmortem proteolysis occurred more rapidly in BM than in LM from both TCC and SB.

Optimization of domes against instability

  • Ye, Jihong;Lu, Mingfei
    • Steel and Composite Structures
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    • 제28권4호
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    • pp.427-438
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    • 2018
  • Static stability is a decisive factor in the design of domes. Stability-related external factors, such as load and supports, are incorporated into structural vulnerability theory by the definition of a relative rate of joint well-formedness ($r_r$). Hence, the instability mechanism of domes can be revealed. To improve stability, an optimization model against instability, which takes the maximization of the lowest $r_r$ ($r_{r,min}$) as the objective and the discrete member sections as the variables, is established with constraints on the design requirements and steel consumption. Optimizations are performed on two real-life Kiewitt-6 model domes with a span of 23.4 m and rise of 11.7 m, which are initially constructed for shaking table collapse test. Well-formedness analyses and stability calculation (via arc-length method) of the models throughout the optimization history demonstrate that this proposed method can effectively enhance $r_{r,min}$ and optimize the static stability of shell-like structures. Additionally, seismic performance of the optimum models subjected to the same earthquake as in the shaking table test is checked. The supplemental simulations prove that the optimum models are superior to the original models under earthquake load as well.

irrE, an Exogenous Gene from Deinococcus radiodurans, Improves the Growth of and Ethanol Production by a Zymomonas mobilis Strain Under Ethanol and Acid Stresses

  • Zhang, Ying;Ma, Ruiqiang;Zhao, Zhonglin;Zhou, Zhengfu;Lu, Wei;Zhang, Wei;Chen, Ming
    • Journal of Microbiology and Biotechnology
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    • 제20권7호
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    • pp.1156-1162
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    • 2010
  • During ethanol fermentation, bacterial strains may encounter various stresses, such as ethanol and acid shock, which adversely affect cell viability and the production of ethanol. Therefore, ethanologenic strains that tolerate abiotic stresses are highly desirable. Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation, ultraviolet light, and desiccation, and therefore constitute an important pool of extreme resistance genes. The irrE gene encodes a general switch responsible for the extreme radioresistance of D. radiodurans. Here, we present evidence that IrrE, acting as a global regulator, confers high stress tolerance to a Zymomonas mobilis strain. Expression of the gene protected Z. mobilis cells against ethanol, acid, osmotic, and thermal shocks. It also markedly improved cell viability, the expression levels and enzyme activities of pyruvate decarboxylase and alcohol dehydrogenase, and the production of ethanol under both ethanol and acid stresses. These data suggest that irrE is a potentially promising gene for improving the abiotic stress tolerance of ethanologenic bacterial strains.

The XRCC1 Arg280His Gene Polymorphism and Hepatocellular Carcinoma Risk: A Meta-analysis

  • Li, Lu-Ping;Wu, Wei;Li, Xing-Hai;Song, Shu-Sen
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권3호
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    • pp.2033-2036
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    • 2013
  • Many studies have suggested that the XRCC1 Arg280His gene polymorphism might be involved in the development of hepatocellular carcinoma (HCC). However, the results have been inconsistent. In this study, the authors performed a meta-analysis to assess the association between XRCC1 Arg280His and HCC susceptibility. Published literature from PubMed, EMBASE and CNKI Data was searched. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using fixed- or random-effects models when appropriate. Begg's test was used to measure publication bias. A total of 7 case-control studies covering 1,448 HCC cases and 1,544 controls were included. No significant variation in HCC risk was detected in any of the genetic models overall. In the stratified analysis, four studies with sample sizes over 300 produced similar results. The corresponding pooled ORs were not substantially altered after the exclusion of three studies deviating from Hardy-Weinberg equilibrium in the control group, which indicated reliability for our meta-analysis results.

Synthesis and Biological Properties of Luotonin A Derivatives

  • Rahman, A. F. M. Motiur;Kim, Dong-Hyeon;Liang, Jing-Lu;Lee, Eung-Seok;Na, Young-Hwa;Jun, Kyu-Yeon;Kwon, Young-Joo;Jahng, Yurng-Dong
    • Bulletin of the Korean Chemical Society
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    • 제29권10호
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    • pp.1988-1992
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    • 2008
  • A series of new derivatives on the ring A of luotonin A were prepared by Friedländer condensation of 6,7,8,10- tetrahydropyrrolo[2,1-b]quinazoline-6,10-dione and suitably substituted 2-aminobenzaldehydes and 2- aminoacetophenones. Their inhibitory activities on topoisomerases and cytotoxicities against selected human cancer cell lines were evaluated. Among the compounds tested, 8-fluoroluotonin A showed similar inhibitory activity on topoisomerase I comparable to camptothecin while luotonin A and 9-hydroxyluotonin A showed 1.37 and 0.94 times stronger inhibitory activity, respectively, on topoisomerase II compared to etoposide. Some derivatives of luotonin A showed moderate cytotoxicity. The possible relationship between the inhibitory activity on Topo II and the cytotoxicity of luotonin A and its analogues, thus, cannot be ruled out.

MIGSHIELD: A new model-based interactive point kernel gamma ray shielding package for virtual environment

  • Li, Mengkun;Xu, Zhihui;Li, Wei;Yang, Jun;Yang, Ming;Lu, Hongxin;Dai, Xinyu
    • Nuclear Engineering and Technology
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    • 제52권7호
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    • pp.1557-1564
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    • 2020
  • In this paper, 3D model-based interactive gamma ray shielding package (MIGSHIELD) is developed in virtual reality platform for windows operating system. In MIGSHIELD, the computational methodology is based on point kernel algorithm (PK), several key parameters of PK are obtained using new technique and new methods. MIGSHIELD has interactive capability with virtual world. The main features made in the MIGSHIELD are (i) handling of physical information from virtual world, (ii) handling of arbitrary shapes radioactive source, (iii) calculating the mean free path of gamma ray, (iv) providing interactive function between PK and virtual world, (v) making better use of PK for virtual simulation, (vi) plug and play. The developed package will be of immense use for calculations involving radiation dose assessment in nuclear safety and contributing to fast radiation simulation for virtual nuclear facilities.

Mechanical Properties of C-type Mesophase Pitch-based Carbon Fibers

  • Ryu, Seung-Kon;Rhee, Bo-Sung;Yang, Xiao Ping;Lu, Yafei
    • Carbon letters
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    • 제1권3_4호
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    • pp.165-169
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    • 2001
  • The C-type mesophase pitch-based carbon fiber (C-MPCF) was prepared throuch C-type spinnerette and compared the mechanical properties to those of round type mesophase pitch fiber (R-MPCF) and C-type isotropic pitch fiber (C-iPCF). The tensile strength and modulus of C-MPCF were about 18.6% and 35.7% higher than those of R-MPCF. The tensile strength of C-MPCF was 62% higher than that of C-iPCF of the same $8{\mu}m$ thickness because of more linear transverse texture, which could be easily converted to graphitic crystallinity during heat treatment. The torsional rigidity of C-MPCF was 2.37 times higher than that of R-MPCF. The electrical resistivity of C-MPCF was $8{\mu}{\Omega}{\cdot}m$. The C-iPCF shows far lower electrical resistivity than R-iPCF as well as the mesophase carbon fiber because of better alignment of texture to the fiber axis.

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Molecular Cloning and Characterization of a Novel Calcium-dependent Protein Kinase Gene IiCPK2 Responsive to Polyploidy from Tetraploid Isatis indigotica

  • Lu, Beibei;Ding, Ruxian;Zhang, Lei;Yu, Xiaojing;Huang, Beibei;Chen, Wansheng
    • BMB Reports
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    • 제39권5호
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    • pp.607-617
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    • 2006
  • A novel calcium-dependent protein kinase gene (designated as IiCPK2) was cloned from tetraploid Isatis indigotica. The full-length cDNA of IiCPK2 was 2585 bp long with an open reading frame (ORF) of 1878 bp encoding a polypeptide of 625 amino acid residues. The predicted IiCPK2 polypeptide included three domains: a kinase domain, a junction domain (or autoinhibitory region), and a C-terminal calmodulin-like domain (or calcium-binding domain), which presented a typical structure of plant CDPKs. Further analysis of IiCPK2 genomic DNA revealed that it contained 7 exons, 6 introns and the length of most exons was highly conserved. Semi-quantitative RT-PCR revealed that the expression of IiCPK2 in root, stem and leaf were much higher in tetraploid sample than that in diploid progenitor. Further expression analysis revealed that gibberellin ($GA_3$), NaCl and cold treatments could up-regulate the IiCPK2 transcription. All our findings suggest that IiCPK2 might participate in the cold, high salinity and GA3 responsive pathways.