• Mycobiology
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• 제48권6호
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• pp.450-463
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• 2020

The strains 17E-042, 17E-039, and NC13-171 belong to Ascomycota and were isolated from soil collected from Sancheong-gun and Yeongam-gun, Korea. The strain 17E-042 produced white mycelial colonies that developed a sienna color with a round margin on potato dextrose agar (PDA), and the reverse side developed a light sienna color. Morphologically, this strain was similar to the strains of Arthrinium phragmites and A. hydei, but the shorter conidial size of the newly identified strain (17E-042) was distinct. The strain 17E-039 produced macroconidia that were pale yellow to orange-brown, elongated-ellipsoid to oblong, round at both ends, primarily straight but sometimes slightly curved, 0-septate, thin-walled, and filled with numerous droplets, having diameters of 20.4-34.3 × 8.0-12.0 ㎛. And the strain NC13-171 formed hyaline to light brown chlamydospores, solitary or in a chain. Multigene phylogenetic analyses were conducted using sequence data obtained from internal transcribed spacer (ITS) regions, 28S rDNA large subunit (LSU), β-tubulin (TUB2), translation elongation factor 1-alpha (TEF1-α), and RNA polymerase II large subunit (RPB2) genes. The results of molecular phylogeny, the detailed descriptions and illustrations of each species strongly support our proposal that these strains from soil in Korea be designated as Arthrinium minutisporum sp. nov. and two new records of Pezicula neosporulosa and Acrocalymma pterocarpi.

• 한국균학회지
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• 제49권1호
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• pp.119-125
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• 2021

등수국은 수국과에 속하는 덩굴성 식물로 우리나라의 울릉도, 제주도, 남해안 섬에 분포한다. 2017년 10월 제주도에서 등수국에 발생하는 녹병균을 국내 최초로 발견하였다. 등수국 녹병균을 동정하기 위해, 형태적 특성을 조사하고 internal transcribed spacer (ITS) 및 28S large subunit (LSU) rDNA의 염기서열의 분자계통학적 분석을 수행하였다. 그 결과, 한국의 등수국 녹병균은 일본과 러시아에서 보고된 Pucciniastrum hydrangeae-petiolaris의 형태적 및 분자계통학적 특징과 일치하였으며, 북미에서 보고된 P. hydrangeae와는 상당히 먼 계통학적 관계를 보였다. 본 연구는 한국의 등수국에서 녹병균 P. hydrangeae-petiolaris의 첫 보고이다.

• 한국균학회지
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• 제49권4호
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• pp.531-537
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• 2021

때죽나무는 때죽나무과에 속하는 낙엽 소교목으로 우리나라와 중국, 일본, 대만이 원산이나 캐나다, 미국, 영국 등에도 도입되어 분포하고 있다. 1990년 이후 전국에 분포하는 때죽나무에서 녹병균이 지속적으로 발견되었다. 한국의 때죽나무 녹병균의 형태적 특성은 일본의 쪽동백나무에서 보고된 Pucciniastrum styracinum의 형태적 특성과 가장 유사하였으며 internal transcribed spacer (ITS) 및 28S large subunit (LSU) rDNA의 염기서열의 분자계통학적 분석을 통해 동정을 확인하였다. 본 연구에서는 한국의 때죽나무에서 발견한 녹병균 P. styracinum의 존재를 재확인하였고, 균학적 특성과 형태적 측정치 및 분자적 특성에 대한 정보를 제공하였다.

• Animal cells and systems
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• 제15권4호
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• pp.315-324
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• 2011

The actiniarian sea anemone, Entacmaea quadricolor, and the scleractinian coral, Alveopora japonica, host symbiotic dinoflagellates belonging to the genus Symbiodinium (Freudenthal). We studied the host-symbiont specificity of these two anthozoan hosts in the northwestern Pacific Ocean. Symbionts within the two hosts were identified using partial large subunit (LSU) ribosomal DNA (rDNA) and complete internal transcribed spacers (ITS) 1 rDNA regions. The host, E. quadricolor, was identified using the partial LSU rDNA molecular marker. Genetic analysis showed that E. quadricolor only harbors dinoflagellates belonging to subclade C1/3 of the genus Symbiodinium. Moreover, no genetic variation was detected among the symbionts of E. quadricolor within the study region (Korea and Japan), even though the two distant sites were separated by more than 1000 km, at collection depths of 1 m in shallow and 13-16 m in deep water. Whilst scleractinian corals host multiple Symbiodinium clades in tropical waters, A. japonica, sampled over a wide geographical range (800 km) within the study region, only hosts Symbiodinium sp. clade F3. The high specificity of endosymbionts in E. quadricolor and A. japonica within the northwestern Pacific Ocean could be accounted for because symbiotic dinoflagellates within the host anemones appear to be acquired maternally, and the Kuroshio Current might affect the marine biota of the northwestern Pacific. However, the consistency of the symbiotic relationships between these two anthozoan hosts and their endosymbionts could change after climate change, so this symbiotic specificity should be monitored.

• Mycobiology
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• 제48권3호
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• pp.184-194
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• 2020

Macrofungi play important roles in forest ecology as wood decayers, symbionts, and pathogens of living trees. For the effective forest management, it is imperative to have a comprehensive overview of macrofungi diversity in specific areas. As a part of the National Institute of Biological Resources projects for discovering indigenous fungi in Korea, we collected macrofungi in Gayasan National Park from 2017 to 2018. These specimens were identified based on morphological characteristics and sequence analysis of internal transcribed spacer (ITS) or the nuclear large subunit rRNA (LSU) region. We discovered 17 macrofungi new to Korea: Butyrea japonica, Ceriporia nanlingensis, Coltricia weii, Coltriciella subglobosa, Crepidotus crocophyllus, Cylindrobasidium laeve, Fulvoderma scaurum, Laetiporus cremeiporus, Lentinellus castoreus, Leucogyrophana mollusca, Marasmius insolitus, Nidularia deformis, Phaeophlebiopsis peniophoroides, Phanerochaete angustocystidiata, Phlebiopsis pilatii, Postia coeruleivirens, and Tengioboletus fujianensis. We described their detailed morphological characteristics.

• 한국균학회지
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• 제48권3호
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• pp.337-343
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• 2020

한국에서 식물병원성 균류의 생물다양성을 파악하기 위한 균류 탐사를 통하여 에키나시아 흰가루병이 지속적으로 관찰되었다. 총 9점의 시료를 고려대학교 식물표본보관소에 보존하였다. 병든 식물에서는 주로 성엽에서 흰색의 얇은 균사층이 전면으로 또는 부분적으로 나타났다. 흰가루병균의 감염으로 줄기와 꽃잎에서는 변색 부위가 나타나는 특징을 보였다. 이 흰가루병균 무성세대의 형태적 특징과 더불어 ITS 및 LSU 염기서열을 계통분석한 결과, 이 흰가루병균은 Podosphaera xanthii로 동정되었다. 이는 한국에서 에키나시아 흰가루병에 관한 최초의 보고이다.

• Mycobiology
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• 제51권6호
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• pp.388-392
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• 2023

A yeast strain, designated as JAF-11^T, was isolated from flower of Prunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11^T was closely related to Neodothiora populina CPC 39399^T with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79^T with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11^T was well-separated from Neodothiora populina CPC 39399^T. In addition to the phylogenetic differences, strain JAF-11^T was distinguished from its closest species, Neodothiora populina CPC 39399^T and Rhizosphaera macrospora CBS 208.79^T belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11^T (KACC 48808^T; MB 850034).

• 한국균학회지
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• 제51권3호
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• pp.251-257
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• 2023

독도에 자생하는 식물인 왕호장근(Fallopia sachalinensis)의 근권 토양에서 다양한 균류를 분리하였다. 분리균들의 형태적 특징과 rDNA-ITS와 LSU 또는 beta-tubulin 영역 분석을 통한 분자적 특징을 확인하였다. 그 결과 기존에 국내에 보고되지 않은 Penicillium striatisporum, Gongronella sichuanensis 종들이 분리되어 이를 보고하고자 한다. 미기록 종들의 형태적 특징과 분자적특징을 본문에 기술하였다.

• ALGAE
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• 제28권3호
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• pp.213-231
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• 2013

Amphidinium massartii Biecheler is an epiphytic and toxic dinoflagellate. Prior to the present study, A. massartii has been reported in the waters off the Mediterranean, Australian, USA, and Canadian coasts. We isolated Amphidinium cells from the coastal waters of Jeju Island, Korea and their morphology and rDNA sequences indicated that they were A. massartii. Herein, we report for the first time the occurrence of A. massartii in the waters of the temperate region in the northwestern Pacific Ocean. The large subunit (LSU) rDNA sequences of the Korean strains were 0.7% different from those of an Australian strain of A. massartii CS-259, the closest species, but were 4.1-5.8% different from those of the other Australian strains and the USA strains of A. massartii and from those of Amphidinium sp. HG115 that was isolated from subtropical Okinawan waters. In phylogenetic trees based on LSU, internal transcribed spacer, small subunit rDNA, and cytochrome b sequences, the Korean strains belonged to the A. massartii clade, which was clearly divergent from the A. carterae clade. The morphology of the Korean A. massartii strains was similar to that of the originally described French strain and recently described Australian strain. However, we report for the first time here that scales were observed on the surface of the flagella. In conclusion, the Korean A. massartii strains have unique rDNA sequences, even though they have a very similar morphology to that of previously reported strains. This report extends the known range of this dinoflagellate to the temperate waters of the northwestern Pacific Ocean.

• 한국균학회지
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• 제48권1호
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• pp.39-45
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• 2020

A fungal isolate US-18-11 was isolated from the soil in Uiseong, Korea. The mycelium growth measured after 7 days of incubation at 22℃ on malt extract agar (MEA) and oatmeal agar (OA) media was 42-43 mm and 41-44 mm in diameter, respectively. The fungal colony formed white to dull green aerial mycelia that were floccose with regular margins and olivaceous black with leaden gray patches on the reverse side. The conidia were hyaline to brown in color, ellipsoidal to ovoid, guttulate, abundant, globose, solitary, or confluent measuring 3.2-7.2×1.1-2.3 ㎛. A BLAST search of the large subunit (LSU), internal transcribed spacer (ITS) region, second largest subunit of DNA-directed RNA polymerase II (RPB2) and β-tubulin (TUB2) gene sequences revealed that the isolate US-18-11 has similarities of 99, 100, 97, and 99% with those of Epicoccum draconis CBS 186.83, respectively. A neighbor-joining phylogenetic tree constructed based on the concatenated dataset of above-mentioned sequences showed that isolate US-18-11 clustered with Epicoccum draconis CBS 186.83 in the same clade. Based on the results of morphological, cultural, and phylogenetic analysis, the isolate US-18-11 was identical to the previously described E. draconis CBS 186.83. To our knowledge, this is the first report of E. draconis in Korea.