• 제목/요약/키워드: LS-1

검색결과 795건 처리시간 0.025초

Microcosm Experiment for Evaluating Efficiency of Chemical Amendments on Remediation of Heavy Metal Contaminated Soil

  • Hong, Young Kyu;Oh, Se Jin;Oh, Seung Min;Yang, Jae E.;Ji, Won Hyun;Kim, Sung Chul
    • 한국토양비료학회지
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    • 제48권2호
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    • pp.138-145
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    • 2015
  • Heavy metal pollution in agricultural field near the abandoned metal mines is a critical problem in Korea. General remediation technique is to apply chemical amendments and soil covering. However, there is no specific guidelines for conducting soil covering. Therefore, main objective of this research was to determine optimum soil covering technique with microcosm experiment. Three different chemical amendments, lime stone (LS), steel slag (SS), and acid mine drainage sludge (AMDS), were examined and varied soil covering depth, 20, 30, 40cm, was applied to determine optimum remediation technique. Bioavailable heavy metal concentration in soil and total concentration of heavy metals in crop were monitored. Result showed that average heavy metal concentration in varied soil covering depth was ordered as 40 cm ($14.5mg\;kg^{-1}$) < 20 cm ($14.6mg\;kg^{-1}$) < 30 cm ($16.0mg\;kg^{-1}$) and also heavy metal concentration in crop was ordered as 40 cm ($100{\mu}g\;kg^{-1}$) < 30 cm ($183{\mu}g\;kg^{-1}$) < 20 cm ($190{\mu}g\;kg^{-1}$). In terms of chemical amendments, average heavy metal concentration was decreased as AMDS ($150{\mu}g\;kg^{-1}$) < SS ($151{\mu}g\;kg^{-1}$) < LS ($154{\mu}g\;kg^{-1}$). Overall, depth of soil covering should be over 30 cm to minimize bioaccumulation of heavy metals and SS and LS could be applied in heavy metal contaminated soil for remediation purposes.

원형질체 내 Plasmid Electroporation에 의한 벼 배발생세포의 형질전환 1. 벼의 Electroporation 원형질체로부터 식물체 재분화 (Transformation of Rice Embryogenic Cells by Electroporation Mediated Plasmid Uptake into Protoplasts 1. Plant Regeneration from Electroporated Protoplasts of Rice)

  • 김명덕;최성진김준철
    • KSBB Journal
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    • 제10권1호
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    • pp.23-29
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    • 1995
  • 발아된 벼(Oryza sativa L. cv. Nakdong)의 하배 축에서 callus를 유도하고, 현탁배양한 세포에서 원 형질체를 분리하여 MS 배지에서 재분화를 수행하였다. Callus는 하배축으로부터 $2.5mg/{\ell}2$,4-0를 첨가한 LS 배지에서 최고 65%의 치상효율로 유도되었으며, 형태척으로 선별 가능한 embryogenic callus 는 $2mg/{\ell}2$,4-D, O.2mgj P kinetin과 $0.1mg/{\ell} GA_3$ 가 첨가된 AA, 배지에서 현탁배양하였다. 4개월 이상 현탁배양한 세포로부터 분리한 원형질체는 voltage를 400V jern와 Imsec 통안 electropora­tion했을 때 $2.5mg/{\ell}2$,4-D, $0.1mg/{\ell}$kinetin과 lOmM proline이 첨가된 PCM 배지에서 1.1%의 치 상효율을 보였다. 형성된 microcalli는 LS2.5 배지 에 치상된 feeder cell에 $0.2\mu\textrm{m}$membrane filter를 얹은 위에 옮겨 암소에서 2주 동안 배양한 후, $30{\pm}/3{\mu}E$.m^{-2}S^{-1}의 형광하에서 2주 통안 배양했을 때 2mm 직경의 노란색 callus를 형성하였다. 형성된 callus를 재분화 배지에 치상하였을 때, 녹점 green spot)에서 shoot 형성과정을 거쳐 완전한 식물체로 분화되었으며 재분화율은 1l~33%였다.

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저장중(貯藏中) 참깨의 결합형태별(結合形態別) 지질(脂質) 및 미량성분함량변화(微量成分含量變化) (Changes in Fractionation Pattern of the Sesame Seed Lipid and Minor Components during Storage)

  • 최상도;조무제
    • Applied Biological Chemistry
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    • 제26권4호
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    • pp.255-260
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    • 1983
  • 산가(酸價)는 모든 조건(條件)에서 저장(貯藏) 4개월(個月)에서 9개월(個月)까지 뚜렷하게 증가(增加)하였다. 그러나 옥소가(沃素價)는 모든 조건(條件)에서 기간(期間)이 길수록 증가(增加)하였고 또한 통기저장(通氣貯藏)이 밀봉저장(密封貯藏)보다 높았다. steryl ester는 기간(期間)이 길수록 LA에서는 증가(增加)되었으나 DS에서는 감소(減少)하였다. triglyceride는 모든 조건(條件)에서 기간(期間)이 길수록 감소(減少)하였으며 DS에서는 감소정도(減少程度)가 미약(微弱)하였으나 LA, DA와 LS에서는 감소정도(減少程度)가 컸다. 야-glycerid는 모든 조건(條件)에서 기간(期間)이 길수록 증가(增加)하였으며 LA가 가장 높고 DS와 LS가 가장 낮았다. free fatty acid는 저장(貯藏) 9개월(個月)까지는 현저(顯著)히 증가(增加)하였다가 9개월(個月) 이후(以後)는 거의 변화(變化)하지 않았다. phospholipid는 모든 조건(條件)에서 기간(期間)이 길수록 감소(減少)하였으며 밀봉저장(密封貯藏)이 통기저장(通氣貯藏)보다 감소정도(減少程度)가 적었다. 유리(遊離) sesamol은 총지방량(總脂肪量)의 $0.0021{\sim}0.0058%$이며 모든 저장조건(條件)에서 저장기간(期間)이 길수록 증가(增加)하였으나 결합(結合) sesamol과 ssamolin은 모든 조건(條件)에서 기간(期間)이 길수록 감소(減少)하였다. sesamin은 총지방량(總脂肪量)의 $0.65{\sim}1.18%$로서 저장조건(條件)에 따른 변화(變化)는 일정(一定)한 경향(傾向)이 없었다.

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푸시 업 자세 시 팔굽관절 각도와 어깨넓이 간격에 따른 구획별 4 배 곧은근 근활성도 비교 (The Comparison of Muscle Activity in 4 Sections of Rectus Abdominis by The Distance of The Shoulder Width and The Angle of The Elbow Joint in The Position of Push-Up)

  • 문교훈;김경훈
    • 대한물리치료과학회지
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    • 제24권1호
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    • pp.14-21
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    • 2017
  • Background; This study was to investigate effects of distance of muscle activity of 4 sections of rectus abdominis(RS-RA, LS-RA, RI-RA, LI-RA) by the shoulder width(x0.5, x1.0, x1.5) and the angle of the elbow joint($0^{\circ}$, $45^{\circ}$, $90^{\circ}$) Method : This study was conducted on 20 healthy male and 20 female adult. respectively, elbow joints were maintained at $0^{\circ}$, $45^{\circ}$, and $90^{\circ}$ flex postures on The shoulder width is 0.5, 1.0, and 1.5 times. at the same time 4 sections of rectus abdominis were measured using EMG with maintaining isometric contraction of the rectus abdominis for 5 seconds. Results; The results were as follows: First, muscle activity of 4 sections of rectus abdominis(RS-RA, LS-RA, RI-RA, LI-RA) by angle of the elbow joint($0^{\circ}$, $45^{\circ}$, $90^{\circ}$) was a statistically significant(p<0.05), Second, muscle activity of 4 sections of rectus abdominis(RS-RA, LS-RA, RI-RA, LI-RA) by the shoulder width(x0.5, x1.0, x1.5) was a statistically non-significant Conclusion; It was found to be most effective to perform arm posture with rectus abdominis muscle strength exercise.

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Vacuum Characteristic of a Chamber Made of Mild Steel

  • Park, Chongdo;Ha, Taekyun;Cho, Boklae
    • Applied Science and Convergence Technology
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    • 제24권4호
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    • pp.84-89
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    • 2015
  • The base pressure and outgassing rate of a mild steel chamber were measured and compared to those of a stainless steel chamber. A combined sputter-ion and non-evaporable getter pump with a nominal pumping speed of 490 l/s generated the base pressure of $2.7{\times}10^{-11}$ mbar in the mild steel chamber and $1.2{\times}10^{-10}$ mbar in the stainless steel chamber. The rate-of-rise measurements show that the mild steel has an extremely low outgassing rate of $2.6{\times}10^{-13}$ mbar $ls^{-1}cm^{-2}$, which is about one-order of magnitude smaller than the outgassing rate of the stainless steels. Vacuum annealing of the mild steel at $850^{\circ}C$ reduced the outgassing rate further to $8.8{\times}10^{-14}$ mbar $ls^{-1}cm^{-2}$, which was comparable to the outgassing rate of a heat treated stainless steel for extreme-high vacuum use.

A rare pseudomyxoma peritonei with a MSH2 variation of unknown significance and two mutation carrier family members

  • Kim, Yoo Min;Kim, Min Kyu
    • Journal of Genetic Medicine
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    • 제13권1호
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    • pp.55-58
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    • 2016
  • Pseudomyxoma peritonei (PMP) is a rare tumor that usually originates in the appendix, but a small number of cases originate in the ovary. Lynch syndrome (LS) is an autosomal dominant hereditary condition that increases the risk of cancer, particularly in the colon and endometrium. Mutations in the mismatch repair genes (MSH2, MLH1, MSH6, and PMS2) increase the risk of LS. Reported PMP cases with hereditary gene mutations of unknown significance are also rare. Here, we investigated a PMP patient and her family members, who have an MSH2 variant of unknown significance. Physicians have an important role in counseling, management, and surveillance based on genetics and pathogenicity.

QP 방법과 McClellan 변환을 이용한 2차원 FIR Elliptic 필터 설계에 관한 연구 (A Study on QP Method and Two Dimensional FIR Elliptic Filter Design with McClellan Transform)

  • 김남수;이상준;김남호
    • 융합신호처리학회 학술대회논문집
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    • 한국신호처리시스템학회 2003년도 하계학술대회 논문집
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    • pp.268-271
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    • 2003
  • There are several methods for the design of 2D filter. Notable among them is McClellan transform method. This transform allows us to obtain a high order 2D FIR filter through mapping the 1D frequency points of a 1D prototype FIR filter onto 2D frequency contours. We design 2D filter using this transform. Then we notice for mapping deviation of the 2D filter. In this paper, Quadratic programming (QP) method allows us to obtain coefficients of McClellan transform. Then we compare deviation of QP method with least-squares(LS) method. Elliptic filter is used for comparison. The optimal cutoff frequencies of a 1D filter are obtained directly from the QP method. Also several problem of LS method are solved.

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북방전복, Haliotis discus hannai의 염분 변화에 따른 생리적 반응 (Physiological Responses in Abalone Haliotis discus hannai with Different Salinity)

  • 신윤경;전제천;임재현;김동욱;손맹현;김응오
    • 한국패류학회지
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    • 제27권4호
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    • pp.283-289
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    • 2011
  • 염분변화에 따른 북방전복의 생리적 반응을 조사하기 위하여 고염분 및 저염분의 농도에 따라 생존율, 삼투질농도, 대사율 및 조직학적 반응을 분석하였다. 염분변화에 따른 북방전복의 생존율은 37 psu에서는 100%였다. 반면 19.8 psu에서 노출 6일째, 12.8 psu 이하에서는 노출 24시간 이내 모두 사망하여 북방전복의 7-days $LS_{50}$은 24.9 psu (20.1-28.2 psu)를 나타내었다. 염분변화에 따른 호흡률은 대조구에 비해 감소하였으며, 반면 암모니아질소 배설률은 증가하였다. 삼투질농도는 염분 26.8 psu이상에서는 1시간 이내 순치하였다. 북방전복 발근육의 조직학적 반응은 염분감소에 따라 혈림프동의 확장, 상피세포의 괴사 및 식세포 증가 등의 현상이 관찰되었다.

Distinctive response of maize (Zea mays L.) genotypes in vitro with the acceleration of phytohormones

  • Muppala, Sridevi;Gudlavalleti, Pavan Kumar;Pagidoju, Sreenu;Malireddy, Kodandarami Reddy;Puligandla, Sateesh Kumar;Dasari, Premalatha
    • Journal of Plant Biotechnology
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    • 제47권1호
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    • pp.26-39
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    • 2020
  • In maize, immature embryos (IEs) are highly regenerative explants most suitable for producing high frequencies of plantlet regeneration in vitro. Apart from media, explants, and hormones, genotypic variation also influences in vitro characters to a great extent. In the present study, IEs were used to study the distinctive effect of variation of size/stage and hormones in different genotypes on five in vitro characters viz., frequency of callus induction, growth rate of total callus, frequency of E. callus induction, and volume and number of regenerated plantlets. LS medium with different concentrations of 2,4-D (0.5, 1.5, 2.5, 4.0 and 5.0 mg/L) were used to study the former four in vitro characters, and medium with 6-benzylaminopurine and kinetin (0.5 mg/L, each) was used for plantlet regeneration. IEs of 1.0, 1.5, 2.0, 2.5 and 3.0 mm in size were isolated from four inbred lines viz., NM74C, NM81A, NM5883 and NM5884. Two-way ANOVA revealed that explant size and genotypes, as well as hormonal concentrations showed significant effects on in vitro characters. Two millimeter IEs were found to be suitable for in vitro cultures. LS medium with 1.5 mg/L 2,4-D and LS with BAP and Kn (0.5 mg/L, each) were found to be the best hormonal concentrations for callus induction, maintenance, and regeneration, respectively. Among the four genotypes, NM81A and NM5883 yielded more non-embryogenic and Type I E. calli. In contrast, NM74C and NM5884 yielded more highly regenerative Type II calli. Inbred line NM5884 was found to be the best among these four genotypes.

암석판재에서 발파공 부근 균열전파에 대한 폭원모델링 (Explosion Modelling for Crack Propagation near Blast holes in Rock Plate)

  • 최병희;강명수;류창하;김재웅
    • 화약ㆍ발파
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    • 제33권1호
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    • pp.13-20
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    • 2015
  • 지하공간의 개발 또는 활용기술에 대한 수요가 전 세계적으로 급증함에 따라, 역학적 안정성과 활용 효율의 측면에서 발파굴착 시 발생하는 손상대 평가는 주요 관심사가 되고 있다. 본 연구에서는 PFC3D와 ANSYS LS-DYNA를 이용하여 판재시료를 대상으로 발파 예정선 주위의 소규모 모형발파에 대한 수치해석을 수행하였다. 또한, 해석 결과의 검증을 위하여 소규모 판재모형을 제작하여 발파실험을 수행하고 균열 전파양상을 비교 고찰하였다.