• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.790-798
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• 2016

Chlamydiae, obligate intracellular bacteria, are associated with a variety of human diseases. The chlamydial life cycle undergoes a biphasic development: replicative reticulate bodies (RBs) phase and infectious elementary bodies (EBs) phase. At the end of the chlamydial intracellular life cycle, EBs have to be released to the surrounded cells. Therefore, the interactions between Chlamydiae and cell death pathways could greatly influence the outcomes of Chlamydia infection. However, the underlying molecular mechanisms remain elusive. Here, we investigated host cell death after Chlamydia infection in vitro, in L929 cells, and showed that Chlamydia infection induces cell necrosis, as detected by the propidium iodide (PI)-Annexin V double-staining flow-cytometric assay and Lactate dehydrogenase (LDH) release assay. The production of reactive oxygen species (ROS), an important factor in induction of necrosis, was increased after Chlamydia infection, and inhibition of ROS with specific pharmacological inhibitors, diphenylene iodonium (DPI) or butylated hydroxyanisole (BHA), led to significant suppression of necrosis. Interestingly, live-cell imaging revealed that Chlamydia infection induced lysosome membrane permeabilization (LMP). When an inhibitor upstream of LMP, CA-074-Me, was added to cells, the production of ROS was reduced with concomitant inhibition of necrosis. Taken together, our results indicate that Chlamydia infection elicits the production of ROS, which is dependent on LMP at least partially, followed by induction of host-cell necrosis. To our best knowledge, this is the first live-cell-imaging observation of LMP post Chlamydia infection and report on the link of LMP to ROS to necrosis during Chlamydia infection.

• Journal of Ocean Engineering and Technology
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• v.13 no.4 s.35
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• pp.112-117
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• 1999

In this paper, the creep properties and creep life prediction by Larson-Miller Parameter method for Udimet 720 to be used for aircraft gas turbine engines or other high temperature components were presented at the elevated temperatures of 538, 649 and $704^{\circ}C$. It was confirmed experimentally and quantitatively that a creep life predictive equation at such various high temperatures was well derived by LMP.

• Composites Research
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• v.15 no.4
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• pp.43-48
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• 2002

This paper presents the brief description of Composite Materials Research carried out at Massachusetts Institute of Technology (MIT). The researches regarding Composites are performed at four Departments, they are Laboratory for Manufacturing ＆ Productivity (LMP) within Mechanical Engineering Department, Technology Laboratory for Advanced Composites (TELAC) within Aerospace Engineering Department, Structures & Materials Program within Civil & Environmental Engineering Department and Building Technology Program within Department of Architecture.

• Journal of Digital Contents Society
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• v.3 no.2
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• pp.143-152
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• 2002

In this paper, we present the general interface, label-switched path structure and link bundling in GMPLS to improve the scalability. The LMP protocol is also introduced to efficiently manage the internal link, and the signaling protocol, hierarchical path setup, hi-directional LSP setup and suggested label method are presented. Finally, the techniques of protection and restoration are presented. In specific, various applicable restoration techniques in GMPLS are discussed.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.194-199
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• 2000

The creep rate is affected by the temperature and in fact. if the temperature above $T_M/2(T_M:melting\;point)$. The aim of the present investigation is to study the relationship of static creep and cyclic creep behavior of pure copper and the formulation of these phenomena with the special attention to the instantaneous strain. strain rate from time and number of cycles have the same inclination Steady state creep rate depend upon maximum stress and can be expressed as linear function according to Power law creep equations Creep rupture time has relation with creep rate. and it make a group represented as the same direct line regardless of max stress, stress ratio and the temperature. Initial strain effect on continuous creep deformation. and have guantitative relationship between elastic and Plastic strain. LMP have similar tendency than OSDP and MHP according to temperature

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2001.05a
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• pp.108-113
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• 2001

In this paper, the real-time prediction of high temperature creep strength and creep strength and creep life for nickel-based superalloy Udimet 720 (high-temperature and high-pressure gas turbine engine materials) was performed on round-bar type specimens under pure load at the temperatures of 538, 649 and 704$^{\circ}C$. The predictive equation of ISM creep has better reliability than that of LMP and LMP-ISM, and its reliability is getting better for long time creep prediction(10$^3$~10sup/5/ｈ).

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.10
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• pp.1902-1910
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• 2009

This paper presents a graphical windows-based program for the education and training for novel network reduction. The object of developed simulator is to provide users with a simple and useable tool for gaining an intuitive feel for power system analysis. The developed simulator consists of the main module (MMI,GUI), the location marginal price module (LMP), the clustering module and network reduction module. Each module has a separate graphical and interactive interfacing window. The developed simulator needs with the PSS/E input data format, generator cost function, location information. Line admittances of reduced network was determined by using the power flow method(Newton-Raphson). So line flow of reduced network is almost same to original power system. Results of reduced network are compared on the window in the tabular format. Therefore, the developed simulator can be utilized as a useful tool for effective education and training for power system analysis.

• Proceedings of the Zoological Society Korea Conference
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• 1997.10b
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• pp.287.2-287
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• 1997

No Abstract, See Full Text

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.10
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• pp.67-78
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• 2003

As optical network technology advances and high bandwidth Internet is demanded for the exponential growth of internet traffic volumes, the Dense-Wavelength Division Multiplexing (DWDM) networks have been widely accepted as a promising approach to the Next Generation Optical Internet (NGOI) backbone networks for nation wide or global coverage. Important issues in the NGOI based on DWDM networks are the Routing and Wavelength Assignment(RWA) problem and survivability. Especially, fault/attack detection, localization and recovery schemes in All Optical Transport Network(AOTN) is one of the most important issues because a short service disruption in DWDM networks carrying extremely high data rates causes loss of vast traffic volumes. In this paper, we suggest a fault/attack management model for NGOI through analyzing fault/attack vulnerability of various optical backbone network devices and propose fault/attack recovery procedure considering Extended-LMP(Link Management Protocol) and RSVP-TE+(Resource Reservation Protocol-Traffic Engineering) as control protocols in IP/GMPLS over DWDM.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10299-10306
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• 2015

The esophageal squamous cell carcinoma (ESCC) is thought to develop through a multi-stage process. Epigenetic gene silencing constitutes an alternative or complementary mechanism to mutational events in tumorigenesis. Posttranscriptional regulation of human leukocyte antigen class I (HLA-I) and antigen processing machinery (APM) proteins expression may be associated with novel epigenetic modifications in cancer development. In the present study, we determined the expression levels of HLA-I antigen and APM components by immunohistochemistry. Then by a bisulfite-sequencing PCR (BSP) approach, we identified target CpG islands methylated at the gene promoter region of APM family genes in a ESCC cell line (ECa109), and further quantitative analysis of CpG site specific methylation of these genes in cases of Kazakh primary ESCCs with corresponding non-cancerous esophageal tissues using the Sequenom MassARRAY platform. Here we showed that the development of ESCCs was accompanied by partial or total loss of protein expression of HLA-B, TAP2, LMP7, tapasin and ERp57. The results demonstrated that although no statistical significance was found of global target CpG fragment methylation level sof HLA-B, TAP2, tapasin and ERp57 genes between ESCC and corresponding non-cancerous esophageal tissues, there was significant differences in the methylation level of several single sites between the two groups. Of thesse only the global methylation level of LMP7 gene target fragments was statistically higher ($0.0517{\pm}0.0357$) in Kazakh esophageal cancer than in neighboring normal tissues ($0.0380{\pm}0.0214$, p<0.05). Our results suggest that multiple CpG sites, but not methylation of every site leads to down regulation or deletion of gene expression. Only some of them result in genetic transcription, and silencing of HLA-B, ERp57, and LMP7 expression through hypermethylation of the promoters or other mechanisms may contribute to mechanisms of tumor escape from immune surveillance in Kazakh esophageal carcinogenesis.