• Journal of Ginseng Research
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• v.23 no.3 s.55
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• pp.190-197
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• 1999

In this study, the molecular mechanism of the growth inhibitory effect of panaxynol was investigated in a human malignant melanoma cell line, SK-MEL-l. In the cell cycle analysis, panaxynol arrested cell cycle progression of SK-MEL-I at the G1 phase. Immunoblot analysis demonstrated that panaxynol increased $p21^{WAF1}$ and decreased cdc2 expression. Protein levels of pl6, p27, E2F-1, Rb, and p53 were not changed. Thus, the changes in expression levels of $p21^{WAF1}$ and cdc2 apparently mediate the cell cycle arrest caused by panaxynol. In addition, cycloheximide (CHX) partially reversed the growth inhibition by panaxynol, which suggested that new protein synthesis was required. On the other hand, LLnL, a proteasome inhibitor, increased antiproliferative effect of panaxynol. This may be due to stabilization of the protein(s) responsible for the growth inhibition such as $p21^{WAF1}$. In summary, these results demonstrate that panaxynol inhibits proliferation of SK-MEL-I by inducing cell cycle arrest at the G1 phase and the inhibitory effect is mediated by the increased level of $p21^{WAF1}$ as well as decreased cdc2 expression.

• The Journal of Korean Society of Virology
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• v.29 no.3
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• pp.155-163
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• 1999

Hantaan virus (HTNV), the etiologic agent of hemorrhagic fever with renal syndrome (HFRS), belongs to the genus Hantavirus, and has three single negative stranded RNA genome segments. HTNV strain Howang isolated from the blood of severe case of Korean HFRS is more virulent than HTNV 76/118 and the M and S genome segments' nucleotide sequence of Howang strain showed 93.5% and 94% homology to each segment of HTNV 76/118. We have obtained 6533 nucleotides long sequence of the L genome segment of Howang strain using reverse transcriptase in conjunction with PCR amplification and compared to other hantaviruses. The messenger sense of the L segment contains one long single long open reading frame of 2151 amino acids, which encodes a deduced RNA dependent RNA polymerase of 246.4 kDa caculated molecular weight protein. The nucleotide sequence of the L segment of Howang strain shows 93%, 74%, 66%, 65% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively. The amino acid sequence of the L segment of Howang strain shows 99%, 85%, 68%, 68% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus $H{\ddot{a}}lln{\ddot{a}}s$ B1 and Sin Nombre virus, respectively.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38B no.9
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• pp.707-714
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• 2013

IoT, which can be regarded as an enhanced version of M2M communication technology, was proposed to realize intelligent thing to thing communications by utilizing Internet connectivity. Things in IoT are generally heterogeneous and resource constrained. Also such things are connected with each other over LLN(low power and lossy Network). Confidentiality, mutual authentication and message origin authentication are required to make a secure service in IoT. Security protocols used in traditional IP Networks cannot be directly adopted to resource constrained devices in IoT. Under the respect, a IETF standard group proposes to use lightweight version of DTLS protocol for supporting security services in IoT environments. However, the protocol can not cover up all of very constrained devices. To solve the problem, we propose a scheme which tends to support mutual authentication and session key agreement between devices that contain only a single crypto primitive module such as hash function or cipher function because of resource constrained property. The proposed scheme enhances performance by pre-computing a session key and is able to defend various attacks.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.5
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• pp.123-128
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• 2019

The Internet Engineering Task Force (IETF) proposed IPv6 Routing Protocol for Low-power Lossy Network (RPL) as a routing protocol for Low Power and Lossy Networks (LLN). In RPL networks, only a few parent nodes are connected to many child nodes, which is called Thundering Herd Phenomenon. To solve this problem, it has been considered to limit the maximum number of child nodes connected per node using the CNC (Child Number Count) parameter. However, the problem remains that some parent nodes can be attached with as many as the maximum number of child nodes. How to determine the maximum CNC value is yet another problem. Therefore, in this paper, we propose an algorithm that evenly distributes the number of child nodes connected per node, to solve the Thundering Herd Phenomenon problem. The performance of the proposed algorithm is compared with that of the conventional RPL using CNC. As a result, we showed that the proposed algorithm performs better in terms of load balancing.

• Journal of Korean Medical classics
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• v.11 no.1
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• pp.2-20
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• 1998

In the field of acupuncture and moxibustion, the theory of taboo of acupuncture and moxibustion on the basis of where the 'Qi and the soul of the men(人氣 人神)' exist became the foundation of the 'theory of chrono-acupunclure and moxibustion(時間性鍼灸法), that takes into account the thrift and retreatment of the Qi(氣) and the 'soul of the men' according to day and time. The fields of acupuncture and moxibustion in the theory of 'Yun Qi(運氣) which takes into account time space are the theories of the 'ZiWuLinZhu(子午流注)' and the 'LingGuiBaFa(靈龜八法)'. While the theory of the 'ZiWuLlnZhu' has the nature of time, 'LingGuiBaFa' has the nature of space comparatively. So the theory of 'Yun Qi' adopted and improved the time-space background that is the basis of the theory of the 'ZiWuLinZhu' and the 'LingGuiBaFa'.

• International Journal of Computer Science & Network Security
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• v.21 no.3
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• pp.212-218
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• 2021

Low-power and Lossy Networks (LLNs) have become the common network infrastructure for a wide scope of Internet of Things (IoT) applications. For efficient routing in LLNs, IETF provides a standard solution, namely the IPv6 Routing Protocol for LLNs (RPL). It enables effective interconnectivity with IP networks and flexibly can meet the different application requirements of IoT deployments. However, it still suffers from different open issues, particularly in large-scale setups. These include the node unreachability problem which leads to increasing routing losses at RPL sink nodes. It is a result of the event of memory overflow at LLNs devices due to their limited hardware capabilities. Although this can be alleviated by the establishment of multi-sink topologies, RPL still lacks the support for effective load balancing among multiple sinks. In this paper, we address the need for an efficient multi-sink load balancing solution to enhance the performance of PRL in large-scale scenarios and alleviate the node unreachability problem. We propose a new RPL objective function, Multi-Sink Load Balancing Objective Function (MSLBOF), and introduce the Memory Utilization metrics. MSLBOF enables each RPL node to perform optimal sink selection in a way that insure better memory utilization and effective load balancing. Evaluation results demonstrate the efficiency of MSLBOF in decreasing packet loss and enhancing network stability, compared to MRHOF in standard RPL.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.6
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• pp.1-6
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• 2014

The IoT (Internet of Things) is considered as a core technology to realize interconnected world. At this, companies composing ICT industry and standard organizations make efforts to accelerate it. IETF CoRE(Constrained RESTful Environment) working group standardized CoAP (Constrained Application Protocol) for the constrained device. CoAP has RESTful architecture and CoAP option is provided to use forward-proxy. The forward-proxy is used to translate protocol and perform requests on behalf of the client. However, communication between Internet based client and LLN(Low-power and Lossy Network) based CoAP server architecture has limitations to deploy real IoT service. In this architecture, problems like response delay, URI assignment and DoS attack can be occurred. To solve these problems, we propose the reverse-proxy based system. We consider both of static IoT and mobility IoT environments. Finally, our proposed system is expected to provide efficient IoT service.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.12
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• pp.1679-1686
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• 2019

The main purpose of this research is to propose an approach for solving the packet loss problem by quickly adapting to topology change when nodes move in RPL-based IoT environment. In order to enhance mobility, every node is aware of the mobility of its neighbor nodes and quantifies the mobility level based on the number of control messages and all received packets. According to the mobility level, the DIO timer is changed. The proposed approach allows nodes to change their DIO timers according to their mobility levels to adapt topology changes and update paths to the sink. The performance of the proposed approach is evaluated using a Contiki-based Cooja simulator in various moving speeds. The simulation results show that the proposed approach copes with mobility scenarios better than the standard RPL by ascertaining that the packet delivery ratio is improved by 31.03%.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.72-77
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• 2001

Expression of f3 ~agarase Gene and Catabolite Repression in Escherichia coli by the Promoter of Alginate Lyase Gene Isolated from Marine Pseudomonas sp. Jin, Cheal~Ho, J~Hyeon Park, Jeong-Hyun Han, YoonM Hyeok Chae, Jong~Hee Lee, Jung-Kee Lee!, and In-800 Kong*. Faculty of Food Science and Biotechnology, Pukyong National UniversitYt Pusan 608-737, Korea, llnBioNet Co. 1690-3 Taejon 306-230, Korea - Promoter is a key factor for expression of the recombinant protein. There are many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter ~md association between the promoter mutants, f3 agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids ofthe alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using peR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of f3 -agarase productivity.

• Journal of Sasang Constitutional Medicine
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• v.25 no.3
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• pp.180-194
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• 2013

Objectives Impaired lung function(ILF) and sasang constitution(SC) is associated with metabolic syndrome(MS). However, the relationship between ILF and SC is unclear. So, we assessed the relationship between ILF and MS according to SC, and examined whether SC is an independent risk factor for ILF. Methods This study included 1,148 subjects 40years and older who were performed the health examination at Kyung Hee University Hospital in Korea between December 2011 and February 2013. We defined ILF if FVC or $FEV_1$ value were less than lower limit of normal(LLN), and classified ventilatory patterns as obstructive pattern and restrictive pattern. MS was assessed according to AHA/NHLBI criteria. We used multivariate logistic regression to analyze the association of ILF with MS respective to SC types, and to identify the risk of SC types for ILF. Odds ratio(OR) was calculated by adjusting for age, sex, smoking status, drinking, physical activity, and BMI. Results In whole subjects, ILF was associated with MS [OR (95% CI), 1.69 (1.24-2.31) for FVC, 1.67 (1.20-2.33) for $FEV_1$]. And in Taeeum type(TE type) and Soeum type(SE type), ILF was associated with MS [1.63 (1.10-2.42) for FVC, 1.48 (1.01-2.24) for $FEV_1$ in TE type; 6.93 (1.14-42.00) for FVC in SE type], while in Soyang type(SY type), it wasn't. The restrictive pattern was associated with MS in TE type and SE type, while in SY type, it wasn't. Furthermore, TE type and SY type had more risk for ILF than SE type [1.71 (1.12-2.59) for SY type, 1.95 (1.23-3.08) for TE type in FVC; 2.06 (1.26-3.36) for TE type in $FEV_1$; 1.85 (1.21-2.85) for SY type, 2.17 (1.35-3.49) for TE type in Restrictive pattern]. Conclusions These results show that SC is an independent risk factor for ILF, especially TE type and SY type than SE type, and the prevalence of MS is an independent risk factor for ILF in TE type.