• 제목/요약/키워드: LINKED SYSTEM

검색결과 1,909건 처리시간 0.03초

Establishment of a NanoBiT-Based Cytosolic Ca2+ Sensor by Optimizing Calmodulin-Binding Motif and Protein Expression Levels

  • Nguyen, Lan Phuong;Nguyen, Huong Thi;Yong, Hyo Jeong;Reyes-Alcaraz, Arfaxad;Lee, Yoo-Na;Park, Hee-Kyung;Na, Yun Hee;Lee, Cheol Soon;Ham, Byung-Joo;Seong, Jae Young;Hwang, Jong-Ik
    • Molecules and Cells
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    • 제43권11호
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    • pp.909-920
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    • 2020
  • Cytosolic Ca2+ levels ([Ca2+]c) change dynamically in response to inducers, repressors, and physiological conditions, and aberrant [Ca2+]c concentration regulation is associated with cancer, heart failure, and diabetes. Therefore, [Ca2+]c is considered as a good indicator of physiological and pathological cellular responses, and is a crucial biomarker for drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to measure [Ca2+]c in single cells and animal models. GECI have some advantages over chemically synthesized indicators, although they also have some drawbacks such as poor signal-to-noise ratio (SNR), low positive signal, delayed response, artifactual responses due to protein overexpression, and expensive detection equipment. Here, we developed an indicator based on interactions between Ca2+-loaded calmodulin and target proteins, and generated an innovative GECI sensor using split nano-luciferase (Nluc) fragments to detect changes in [Ca2+]c. Stimulation-dependent luciferase activities were optimized by combining large and small subunits of Nluc binary technology (NanoBiT, LgBiT:SmBiT) fusion proteins and regulating the receptor expression levels. We constructed the binary [Ca2+]c sensors using a multicistronic expression system in a single vector linked via the internal ribosome entry site (IRES), and examined the detection efficiencies. Promoter optimization studies indicated that promoter-dependent protein expression levels were crucial to optimize SNR and sensitivity. This novel [Ca2+]c assay has high SNR and sensitivity, is easy to use, suitable for high-throughput assays, and may be useful to detect [Ca2+]c in single cells and animal models.

부하유무에 따른 수영운동이 중추신경계 손상 흰쥐의 혈청 BDNF 농도 및 행동변화에 미치는 영향 (The Effect of the Swimming Exercise by Load on Concentration of BDNF in Serum and Behavioral Change of CNS Injury in the Rats)

  • 하미숙;형인혁
    • 한국콘텐츠학회논문지
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    • 제9권12호
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    • pp.391-399
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    • 2009
  • 본 연구의 목적은 중추신경계 손상의 쥐에 운동강도에 따른 신경계 회복을 알아보기 위해 부하수영과 무부하수영이 혈청 BDNF 농도와 행동학적 변화에 어떠한 영향을 미치는가를 확인하고자 하였다. 실험동물로는 Sprague-Dawley 수컷 흰쥐를 사용하였으며, Ketamine을 복강 내에 주사하여 마취한 뒤 제 1-2요추의 척수에 6-OHDA를 $100{\mu}{\ell}$ 주입하여 척수손상을 유발시켰다. 척수 손상으로 유발된 행동학적 변화를 알아보기 위해 BBB와 경사판 검사를 기록하였다. 혈청 BDNF는 수술 후 14일에 혈액을 채취해 흡광도를 측정하였다. 척수 손상 후 무부하군에서는 행동변화가 대조군에 비해 유의하게 증가하였고, 혈청 BDNF 농도는 다른 군에 비해 무부하군에서 증가하였다. 이러한 결과로 보아 저강도 수영운동을 중추신경 손상 후 초기에 적용하면 신경조직의 회복에 영향을 주리라 생각된다.

효율적인 의사결정을 위한 이벤트 기반의 프로세스 성과측정을 위한 모델 (Process Performance Measurement Model Based on Event for an efficient Decision-Making)

  • 박제원;최재현;조풍연;이남용
    • 정보처리학회논문지D
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    • 제17D권4호
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    • pp.259-270
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    • 2010
  • 최근 정보시스템은 정보기술의 발달로 이기종의 환경으로 분산되어 프로세스를 중심으로 복잡하게 얽혀 있다. 프로세스는 어떠한 업무가 시작되어 끝날 때까지 의사결정의 선후관계를 갖는 인스턴스의 흐름을 가지고 여러 정보시스템을 넘나들며, 업무의 성과를 측정하는데 필요한 많은 정보를 담고 있다. 프로세스는 단위 업무활동인 작업으로 구성되며, 단위 작업은 정보 발생원인 이벤트로 구성된다. 그러나 대부분의 경우 직접 이벤트를 연계하여 프로세스의 진행이 경영적인 관점에서 얼마나 성과 있게 진행되고 있는지를 측정하는 것은 매우 어렵다. 따라서 프로세스가 완전히 끝나기 전에 작업의 진행에서 프로세스의 성과를 미리 측정한다면 운영비용 절감 및 효율적인 프로세스 실행이 가능하다. 이에 본 논문에서는 프로세스의 현재 성과를 사전에 평가할 수 있도록 이벤트 기반의 프로세스 성과측정 모델을 제안하였다. 첫째, 프로세스 성과측정개념을 제시하고, 단위 정보시스템의 이벤트로부터 수집된 정보를 통해 프로세스의 성과측정을 위한 성과지표 선정방법을 연구한다. 둘째, 프로세스 성과측정을 위한 이벤트 수집을 위해 선정된 성과지표와 기존 시스템의 이벤트 간 매핑방법과 수집된 이벤트를 프로세스 관점에서 효율적으로 저장관리 하는 데이터 스키마와 방법을 연구한다. 마지막으로 수집된 이벤트를 바탕으로 프로세스의 성과 측정 모델을 제시한다.

3차원 흐름모델을 이용한 지하저수지의 관리에 대한 연구 (A Study of the Management of Groundwater Reservoir by Numerical Three Dimensional Flow Model)

  • 신방웅;김희성
    • 지질공학
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    • 제5권3호
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    • pp.289-300
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    • 1995
  • 지하 저수지를 효과적으로 관리하기 위하여는 지표. 지하 수문 특성,대수층계의 수리지질 특성, 차수벽의 차수 기능을 기본 설계 단계부터 계획적으로 분석 검토하여야 한다. 본 연구에서는 국재 최초로 건설된 경북 이안 지하 저수지의 기능 분석하기 위하여 3차원 유한 차분 흐름모델을 이용하였다.지하댐 건설 후의 정류모의를 구축하였으며 그 결과 각 조건에 따른 지하 수문계의 물수지를 결정하고 안전 채수량을 제시하였다. 모의 결과 지하수위의 계절적 변동에 영향을 주는 주요인의 하나로 관개용수의 지하침투 현상이 규명되었다. 관개용수로부터 함양량은 5,6개월에는 4.3mm/d, 7,8월에는 1.7mm/d이다. 유역 산지로부터 대수층내로의 함양량은 $0.04m^3/s$로서 연중일정하며 1984년 댐의 종단면을 통한 지하수 유출량이 $0.002m^3/s$일 때 관개 기간인 7,8월 두달간 최적 양수량은 $254000m^3$이었으나, 1993년 댐의 종단면을 통한 지하수 유출량은 $0.013m^3/s$로서 차수벽의 기능은 상실되었다. 지하댐의 차수기능을 보강하지 않을 경우 관개 기간내에서의 최적 양수량은 $93,000m^3$이 된다.

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인간자궁내막의 탈락막화 (Decudualization)에 있어서 TGF-$\beta$ (Transforming Growth Factor-$\beta$)의 역할 (Transforming Growth Factor-$\beta$ is a Possible Paracrine Mediator in the Human Endometrial Decidualization)

  • 박동욱;최동순;김미란;황경주;조미영;안성희;민철기;유희석
    • Clinical and Experimental Reproductive Medicine
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    • 제30권1호
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    • pp.65-75
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    • 2003
  • Objectives: To investigate the role of TGF (Transforming growth factor-$\beta$) involved in the paracrinic communication during decidualization between UEC (uterine epithelial cells) and USC (uterine stromal cells), we have employed a co-culture system composed of human endometrial epithelial and stromal cells in defined hormonal conditions. Design: In the co-culture, endometrial epithelial cells cultured in the matrigel-coated cell culture insert are seeded on top of the endometrial stromal cells cultured within a collagen gel. The co-culture was maintained for 48 hours under the following hormonal conditions: progesterone dominant condition (100 nM P4 and 1 nM E2) or estrogen-dominant condition (100 nM E2 and 1 nM P4). 10 ng/ ml HGF and/or 10 ng/ml TGF-$\beta$1 are added. Methods: RT-PCR is utilized to detect mRNAs quantitatively. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining are utilized to detect proteins in the tissue. Results: Prolactin mRNA is expressed in the co-cultured stromal cells under the progesterone dominant condition. TGF-$\beta$1 and its receptors are expressed in both the co-cultured epithelial and stromal cells irrespective of the steroid present, which is in contrast with no or negligible expression of TGF-$\beta$1 or its receptor in cells separately cultured. Both estrogen and progesterone significantly elevate the concentration of hepatocyte growth factor (HGF) in the conditioned medium of the co-culture with the value of 4, 325 pg/ml in E2-dominant and 2, 000 pg/ml in P4-dominant condition compare to 150 pg/ml in no hormone. In separately cultured stromal cells, administration of HGF induces the expression of TGF receptor 1 in both hormonal conditions, but induction of TGF receptor 2 is only manifest in the P4-dominant condition. Administration of TGF-$\beta$ and HGF directly induce the decidualization marker prolactin mRNA in separately cultured stromal cells. Conclusion: It is likely that steroid hormones induces prolactin mRNA indirectly by promoting the cell to cell communication between the stromal and the epithelial cells. TGF-$\beta$ and HGF are two possible paracrine mediators in the human endometrial decidualization.

Ecological Functions and Losses of Traditional Korean Village Groves

  • Lee, D.-W.;Park, C.-R.
    • 한국제4기학회지
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    • 제18권2호통권23호
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    • pp.65-66
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    • 2004
  • There have been groves, in many cases, along with hedgerows and remnant forests around a traditional Korean village. A village grove is very closely connected to the life of residents. Sometimes it was a holy place where important village festivals were held, and became a resting place for farmers, especially in sunny summer. As a matter of fact, it is noted that traditional Korean village groves had been fostered for many purpose as religion, Confucianism, scenery, sanitation, traffic guard, public security, agriculture, hunting, and military and public uses were included in Chosun Govemor General(1938). Village groves were usually located at the outlet of watershed inside which a village was built. In addition, village groves used to be established along part of mountain ranges, streams and streets. A unique type of village grove, called bibosoop was fostered especially where the outlet of watershed was largely opened. In other cases, it was placed where a part of mountain range was relatively low, or where village residents were likely to see ugly objects such as a huge cliff, stony upland with an unvegetated area and the like(Kim and Jang 1994). In a sense, a sheltebelt is a sort of bibosoop as it is a landscape element to complement places that are exposed to strong winds. However, it is comparable to other typical bibosoop that is situated at a topographically very specific zone of watershed. In this paper, we will address potential functions of Korean village groves from a perspective of modern landscape ecology and show current status of some remnants, based on preliminary surveys. A village grove functions as barrier or filter of objects such as water, nutrients, and other elements and habitat of wildlife (park et al. 2003, Lee 2004). The village grove slows down the flow of water and air, maintains soil moisture an hinders soil erosion, enabling cultivation of crops and bringing up creatures nearby. It contributes to enhancing biodiversity. Birds rest on shrubby and woody trees of the element. Presumably, other organisms may also inhabit the village groves and take advantage of it when those move from a forest patch to others. Emerging insects acclimate themselves in the shade of the green space before they fly to sunny air. Besides the village grove acts as a component of agroforestry system as leaf litter is shed from a grove to an asjacent agricultural area, and transformed into green manure(Lee 2004). By the way, many of the landscape elements were destroyed or declined in Koea during the past several decades. The losses have been parallel or linked to environmental degradation. Unfortunately, we have a little reliable data as for how many groves have disappeared in Korea until now. There has been no complete census on the village groves in Korea, and the viewpoints of survey were to a degree different depending on surveyors. So, it is difficult to analyze the temporal and spatial change of village groves. Currently, national inventory data of Korean village groves are available in three reports. We reviewed the locations of village groves and arranged those according to the present administrative units, DONG. With the limited data, we found that at least 484 of village groves were recorded in South Korea. Among all provinces, village groves were most in Gyeongsanbuk-Do Province and least in Chungcheongbuk-Do Province(Table 1). This is a preliminary report prepared while some quantitative data regarding functions and lossers of the village groves are being collected. More detailed data will be introduced in the near future.

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동중국해에 있어서 어족생물의 일주기적 유영행동특성에 관한 연구-I-획야 전이시간대의 유영행동특성- (Hydroacoustic Observations on the Diel Distribution and Activity Patterns of Fishes in the East China Sea II -Activity Patterns during the Evening and Morning Transition Periods -)

  • 이대재
    • 수산해양기술연구
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    • 제30권4호
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    • pp.239-250
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    • 1994
  • The vertical distribution and activity patterns of fishes during the evening and morning transitions between day and night were studied acoustically and by bottom trawling in November 1990-1992 in thermally stratified waters of the East China Sea. The acoustic data were collected from six stations with a scientific echo-sounder operating at two frequencies of 25 and 100kHz, and the echograms were used to determine the vertical distributions of fish. Biological sampling was accomplished by bottom trawling to identify fish species recorded on the echograms, and the species and length compositions were determined. At each station, vertical profiles of water temperature, salinity and dissolved oxygen were taken with a CTD system and were related to the diel movements and the depth distributions of fish. During the day most fish were within several meters above bottom, but began to migrate upwards just before sunset, and during the night they were dispersed in midwater. Prior to sunrise with a thermocline present, one group of the fish aggregation occurred in dense schools slightly above the thermocline, while the other group occurred with the numerous single fish-traces bellow it. These groups of aggregations rapidly began to migrate toward the bottom across the thermocline from about 40 min before sunrise. Trawl hauls in the bottom strata below the thermocline with the characteristic single fish traces yieled invariably catches dominated by snailfish and fishing frog with minor quantities of other species in all stations. Hence, the results indicate that snailfish and fishing frog were the dominated scatterers in the depth strata below the thermocline, and the single-fish recordings were mainly snailfish. The fish species such as anchovy and juvenile mackerel in bottom trawl catches is poorly represented in relation to the mesh selectivity of the trawl net, but their occurrence suggest that the fish-school recording above the thermocline were due to these species which migrated vertically across the thermocline, with a temperature gradient of about 8$^{\circ}C$, from the water layers near the bottom at night. Accordingly, we conclude that the vertical distribution and activity patterns of snailfish were strongly temperature dependent and in the termally stratified waters, the upper limit to diel activity was closely linked to the position of the thermocline.

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1999년과 2000년 여름몬순기간 동안 히말라야 지역에 발생한 대류계의 특성에 관한 연구 (Characterization of Convective Weather Systems in the Middle Himalaya during 1999 and 2000 Summer Monsoons)

  • 김광섭;노준우
    • 한국수자원학회논문집
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    • 제36권3호
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    • pp.495-505
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    • 2003
  • Meteosat-5 IR 위성영상을 사용하여 1999년과 2000년 여름몬순기간 동안 발생한 네팔과 인디아 북쪽 히말라야 산악지역에 발생하는 여러 형태의 대류계 즉, 중규모 대류계들 (Mesoscale Convective Complex, MCC and Convective Cloud Clusters, CCC) 와 보다 약한 Disorganized Short-lived Convection (DSL)의 이동특성 및 시공간적인 생성특성 등을 조사하였다. 대상지역에 발생하는 중규모 대류계의 전형적인 지속시간은 약 11시간이며 크기는 약 $300,000km^2$ 이다. 중규모 대류계의 중심은 히말라야산맥으로부터 원거리에 위치함에도 불구하고 집중강운-는 위도 $25^{\circ}-30^{\circ}N$ 사이의 히말라야 하단에 발생하는 중규모 대류계와 직접적 상관관계를 가진다. 결과는 히말라야 고도 500-4000m에 설치된 강우계로부터 획득된 강우자료의 변화 특성과 대류계 거동 특성이 유사함을 보여주었다(Barros et al. 2000). 집중호우의 강력한 야간발생과 Gangetic Plains에서 발생한 중규모 대류계와의 연관성을 보여주었다(Barros et al. 2000).

위암 및 전립선암의 종양 표지 인자로서 혈장 TGF-β1에 대한 연구 (Study of plasma TGF-β1 level as a useful tumor marker in gastric cancer and prostate cancer)

  • 임창기;신훈;최인영;정병하;류민희;방영주;진승원
    • IMMUNE NETWORK
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    • 제1권3호
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    • pp.260-265
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    • 2001
  • Transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$) is a multipotent growth factor affecting development, homeostasis and tissue repair. Many kinds of malignant tissues were reported to overexpress transforming growth $factor-{\beta}1$ ($TGF-{\beta}1$) gene. However, a little work has been done on the circulating $TGF-{\beta}1$ and the association of $TGF-{\beta}1$ with progression in patients with malignant tumors. In this study, we measured the plasma level of $TGF-{\beta}1$ in gastric cancer and prostate cancer patients and evaluated the utility of plasma $TGF-{\beta}1$ as a possible tumor marker. We used Enzyme-linked immunosorbent assay (ELISA) system in order to measure plasma $TGF-{\beta}1$ level in 134 gastric cancer patients, 50 prostate cancer patients and 290 normal controls. And the tumor marker, carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), was compared with $TGF-{\beta}1$ in the aspects of sensitivity and specificity. The mean plasma $TGF-{\beta}1$ levels were $1.219{\pm}0.834$ (0.272-5.772) ng/mL in normal controls, $5.964{\pm}3.218$ (0.845-18.124) ng/mL in gastric cancer and $4.140{\pm}2.345$ (1.108-13.302) ng/mL in prostate cancer. In gastric cancer patients difference in plasma $TGF-{\beta}1$ level was not detected according to cancer stage. In comparison with other tumor marker (CEA, PSA) $TGF-{\beta}1$ is more potent in sensitivity. These results indicate that the plasma $TGF-{\beta}1$ level can be a potent tumor marker in gastric cancer and prostate cancer.

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SWIFTNet TSU BPO의 계보학적 연구 (The Genealogical Study on SWIFTNet Trade Service Utility and Bank Payment Obligation)

  • 이봉수
    • 통상정보연구
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    • 제18권3호
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    • pp.3-21
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    • 2016
  • 본 연구는 SWIFTNet TSU 선행연구의 고찰과 함께 지원업무의 내용을 살펴본 후, 이를 토대로 전자무역에 활용하기 위한 전략점 시사점을 분석하였으며 그 결과 얻어진 내용은 다음과 같다. 첫째, SWIFTNet TSU BPO의 거래를 개시할 때에 은행과 수입업자가 교환한 거래약정서에서 은행의 채권보전에 관한 규정을 설정하는 조치가 필요하다. 아울러 송금방식의 결제보다 비용은 더 들어가지만 SWIFTNet TSU BPO는 수출자에게 자금공여를 해주는 장점을 가지므로 수입자의 입장에서는 수출자와 매매계약을 할 때 제품 가격 인하의 주도권을 확보할 수 있다고 보았다. 둘째, SWIFTNet TSU BPO도 단일화된 공유기반의 플랫폼을 구축함으로써 보안성과 안정성 및 편리성을 갖추어야 한다. 이를 위하여 은행은 업무처리에 대한 서비스를 제공하기 위한 새로운 시스템을 구축할 필요가 있다. 나아가 SWIFTNet TSU BPO가 완전한 글로벌 시스템으로 완성되기 위해서 전세계의 무역거래자들이 만족할 수 있는 획기적인 결제 솔루션으로 거듭나야 하며 전자무역 결제시스템을 위한 기술적 표준화가 선결되어야 한다. 셋째, SWIFTNet TSU BPO를 은행결제와 다른 부가가치의 무역서비스 기능과 융합시키며 신속성, 외환결제 리스크 경감, 편리성, 비용절약 측면에서 우위성을 갖고 있으므로 여러 국가에서 더욱 편리하게 사용할 수 있도록 은행과 수출입업자, SWIFT의 협력 강화가 이루어져야 한다. 넷째, 무역유관기관들은 빠른 시일안에 은행지급확약통일규칙(URBPO)에 관한 지식의 습득이 요구되며 제도적으로 신속하게 확산될 수 있도록 글로벌 차원의 노력이 필요하다. 이와 함께 참여은행들에게는 거래 수수료에 대한 충분한 보상도 이루어져야 한다.

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