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Analyses of the Effect of Inserting Border Lines between Adjacent Color Regions on Detecting Boundaries (경계선 검출에 대한 인접 칼라 영역간 테두리 선 삽입 효과의 분석)

  • Yoo, Hyeon-Joong;Kim, Woo-Sung;Jang, Young-Beom
    • Journal of IKEEE
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    • v.10 no.1 s.18
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    • pp.87-95
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    • 2006
  • This paper presents the analyses of the effect of inserting border lines between different color regions on edge detection in color codes, and is not intended to present any new algorithm for color-code recognition. With its role to complement the RFID (radio frequency identification) and the wide and fast spread of digital cameras, an interest on color codes is fast increasing. However, the severe distortion of colors in obtained images prohibits color codes from expanding their applications. To reduce the effect of color distortion it is desirable to process the whole pixels in each color region statistically, instead of relying on some pixels sampled from the region. This requires segmentation, and the segmentation usually requires edge detection. To help detect edges not disconnected, we inserted border lines of the width of two pixels between adjacent color regions. Two colors were used for the border lines: one consisting of white pixels, and the other black pixels. The edge detection was performed on images with either of the two kinds of border lines inserted, and the results were compared to results without inserted border lines. We found that inserting black border lines degraded edge detection by causing zipper effect while inserting white border lines improved it compared to the cases without inserted border lines.

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Selection of 5-Methyltryptophan and S-(2-Aminoethyl)-L-Cysteine Resistant Microspore-Derived Rice Cell Lines Irradiated with Gamma Rays

  • Kim, Dong-Sub;Lee, In-Sok;Jang, Cheol-Seong;Hyun, Do-Yoon;Lee, Sang-Jae;Seo, Yong-Weon;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.33-41
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    • 2003
  • Microspore-derived cell lines resistant to 5-methyltryptophan (5MT, a tryptophan analog) or S-(2-aminoethyl)-L-cysteine (AEC, a Iysine analog) were selected in rice by in vitro mutagenesis. For selection of 5MT or AEC resistant cell lines, suspension-cultured cells were irradiated with gamma rays. Thirteen 5MT resistant cell lines were selected and they were able to grow stably at 2 times higher 5MT concentration. A feedback insensitive form of anthranilate synthesis, the pathway specific control enzyme for tryptophan synthesis, was detected from the 5MT resistant lines. Contents of the free amino acids in five resistant lines (MR12-1 to MR12-5) showed a 7.4 to 46.6 times greater level than that in the control culture. Tryptophan, phenylalanine, and tyrosine levels in the shikimate pathway were 28.1 and 22.5 times higher in MR12-3 and MR12 4, respectively, than that measured in the control cells. Four AEC resistant cell lines were isolated from cultures grown on medium containing 1 mM AEC, They were able to grow stably with 2 mM AEC, while sensitive calli were inhibited by 0.5 mM AEC. Aspartate kinase activities of the resistant lines were insensitive to the natural inhibitor, Iysine, and accumulated 2.2 to 12.9-fold higher levels of free Iysine than that of the control cells. Especially, the levels of aspartate, asparagine, and methionine in the aspartate pathway showed higher accumulation in the AEC resistant lines than that in the control cells.

Establishment and Characterization of Clonal Cell Lines from Zebrafish, Danio rerio (제브라피쉬(Danio rerio) 배아로부터 동형세포주 확립)

  • Lee, Ki-Young
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.1-6
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    • 2008
  • Three types of clonal cell lines were isolated according to their size and phenotype from the adherent cell populations in long-term liquid cultures from the embryonic fibroblast cells of Zebrafish, Danio rerio. All kind of cell lines were well proliferated. The size and number of clonal cell lines derived colonies from stable embryonic cells were significantly increased in the presence of NAC and A2P conditioned medium from the cell lines. The stable cell lines and clonal cell lines were cap-able of well proliferation in vitro. These cell lines have been maintained in continuous culture without change in characteristics. A majority of the clonal cells (80%) was shown a normal chromosomal complement (50 chromosomes, 2N) in according with FACs analysis. Majority of cells were positive to vimentin staining and none of them were positive for nestin and Oct -4 by immunocytochemistry. These results indicate that the clonal cell lines obtained from cultured cells are fibroblasts and may be extremely useful in genetic manipulation for further nuclear transfer and fish cloning.

Study of Ojayeonjonghwan on hydrogen peroxide-induced oxidative stress in male reproductive GC-1 germ cell lines (Hydrogen peroxide에 의해 유도된 남성 생식 세포 GC-1 cell에 미치는 오자연종환(五子衍宗丸)의 효과 연구)

  • Chang, Mun Seog;Lee, Ho Chul;Lee, Seung Ho;Park, Seong Kyu
    • Herbal Formula Science
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    • v.29 no.1
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    • pp.1-8
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    • 2021
  • Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines. Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition. Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 ㎍/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 ㎍/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 ㎍/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed. Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.

A Study on the Productivity Changes of the Korean Container Shipping Lines using MPI (MPI를 활용한 국적 외항 컨테이너 선사의 생산성 변화 분석 연구)

  • Sung Sub, Shin;Chi Yeol, Kim;Min-Ho, Ha
    • Journal of Navigation and Port Research
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    • v.46 no.6
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    • pp.547-553
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    • 2022
  • This study analyzed the changes in the operational productivity of fourteen Korean container lines from 2019 to 2021 using MP I(Malmquist Productivity Index). The results indicated that the operational productivity of the shipping companies has increased by 38.4% annually, representing the TCI (Technical Change Index) increasing by 58.3% and the TECI (Technical Efficiency Change Index) decreasing by 12.6%. The increase in the operational productivity of the container shipping lines was mainly attributed to the high rise in ocean freight rates rather than an increase in fleet size or ship technical efficiency. However, the deep-sea shipping lines (i.e. HMM and SM lines) experienced increases in both the TCI and TECI, which was not the case for other shipping lines(i.e. Intra-Asian short-sea shipping lines). The intra-Asian short-sea shipping lines enhance their productivity due to the TCI but failed to appreciate the cost savings of the increased fleet effects due to the low SECI(Scale Efficiency Change Index) values.

Comparison of the Quality Characteristics of the Rice yield Trial Lines in the Central Plain Region for Four Years

  • Jeong Heui Lee;Jieun Kwak;Hyun-Jin Park;You-Geun Oh;Jeom-Sig Lee;Yu-Chan Choi;Seon-Min Oh
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.325-325
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    • 2022
  • In order to developing high-quality rice varieties and processing varieties, systematic and stable evaluation of physicochemical properties is required for breeding lines. In this study, we compared the quality characteristics evaluation results of rice breeding lines for cooked rice, special rice, and whole crop silage rice adapted to central plain region (Suwon) in order to use as basic data for future rice variety development. Brown rice length/width ratio, head rice ratio, protein content, amylose content, alkali digestion value(ADV) and Toyo value were analyzed to evaluate the quality characteristics of yield trial lines cultivated in Suwon for four years (2017-2020). Brown rice length/width ratio, head rice ratio, protein content, ADV and Toyo values showed significant differences by year, but there was no significant difference in amylose content (p<0.05), which showed little environmental variation. The head rice ratio and Toyo value showed an increasing trend, while the protein content showed a decreasing trend. However, the protein content was the highest in 2020, which is thought to be owing to little sunlight hours due to heavy rainfall in 2020. The protein content of whole crop silage rice was 8.1%, which was significantly higher than that of other lines (p<0.05). Toyo value of medium-maturing and early-maturing lines were 67.6 and 73.7%, respectively, and the Toyo value of medium-maturing lines was higher than that of the early-maturing lines (p<0.05). In correlation analysis among the quality characteristics of the rice lines for cooked rice, significant positive correlations were detected between Toyo value and head rice ratio, amylose content, ADV, and a negative correlation was observed between Toyo value and protein content (p<0.05).

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Clothing Pressure and Subjective Sensations in Slacks using the Cutting Lines of the Knee Parts (슬랙스의 무릎절개선 활용에 따른 의복압 및 착용감)

  • Lee, Joung-Suk;Sung, Su-Kwang
    • Fashion & Textile Research Journal
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    • v.6 no.1
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    • pp.109-113
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    • 2004
  • The present study attempted to consider the effects of cutting lines in the knee parts of slacks on the clothing pressure and comfort. The study compared and analyzed the pressure imposed to the body in a stooping position and the consequent sensations felt by the wearer according to the slack shapes. The conclusion was drawn as follows: The pressure imposed by the slacks to the body is greater on the two points slightly upper and lower from the knee point than on the knee point itself, regardless of the slack shapes. Clothing pressure was lower in the slacks with 10 cm-wide and 14 cm-wide cutting lines in the knee parts than those with 18 cm-wide cutting lines. Subjective sensations felt in the knee parts were analyzed according to slacks shapes. As a result, it was found that slacks without cutting lines on the knee parts or with sewing lines far from the knee points were more comfortable. In slacks construction, the use of cutting lines in the knee parts can decrease pressure imposed to the knee area or improve the comfort of the slacks. When designing slacks, however, overall appearance should be considered and the weakness of cutting line that makes legs look shorter should also be complemented.

Vanishing Point Detection using Reference Objects

  • Lee, Sangdon;Pant, Sudarshan
    • Journal of Korea Multimedia Society
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    • v.21 no.2
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    • pp.300-309
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    • 2018
  • Detection of vanishing point is a challenging task in the situations where there are several structures with straight lines. Commonly used approaches for determining vanishing points involves finding the straight lines using edge detection and Hough transform methods. This approach often fails to perform effectively when there are a lot of straight lines found. The lines not meeting at a vanishing point are considered to be noises. In such situation, finding right candidate lines for detecting vanishing points is not a simple task. This paper proposes to use reference objects for vanishing point detection. By analyzing a reference object, it identifies the contour of the object, and derives a polygon from the contour information. Then the edges of the detected polygon are used to find the vanishing points. Our experimental results show that the proposed approach can detect vanishing points with comparable accuracy to the existing edge detection based method. Our approach can also be applied effectively even to complex situations, where too many lines generated by the existing methods make it difficult to select right lines for the vanishing points.

Automatic Assembly Task of Electric Line Using 6-Link Electro-Hydraulic Manipulators

  • Kyoungkwan Ahn;Lee, Byung-Ryong;Yang, Soon-Yong
    • Journal of Mechanical Science and Technology
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    • v.16 no.12
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    • pp.1633-1642
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    • 2002
  • Uninterrupted power supply has become indispensable during the maintenance task of active electric power lines as a result of today's highly information-oriented society and increasing demand of electric utilities. The maintenance task has the risk of electric shock and the danger of falling from high place. Therefore it is necessary to realize an autonomous robot system using electro-hydraulic manipulator because hydraulic manipulators have the advantage of electric insulation. Meanwhile it is relatively difficult to realize autonomous assembly tasks particularly in the case of manipulating flexible objects such as electric lines. In this report, a discrete event control system is introduced for automatic assembly task of electric lines into sleeves as one of the typical task of active electric power lines. In the implementation of a discrete event control system, LVQNN (linear vector quantization neural network) is applied to the insertion task of electric lines to sleeves. In order to apply these proposed control system to the unknown environment, virtual learning data for LVQNN is generated by fuzzy inference. By the experimental results of two types of electric lines and sleeves, these proposed discrete event control and neural network learning algorithm are confirmed very effective to the insertion tasks of electric lines to sleeves as a typical task of active electric power maintenance tasks.

Genetic Variation of a Single Pollen-derived Doubled Haploid Population in Rice

  • Moon, Huhn-Pal;Kang, Kyung-Ho;Ahn, Sang-Nag;Choi, Seon-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.4
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    • pp.250-253
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    • 1998
  • Somaclonal variation was observed in the field on doubled haploid plants derived from single pollen of a rice cultivar "Hwaseongbyeo". The variations of seven quantitative traits including plant height and one qualitative trait (pubescence) in 436 lines ($R_2$ generation) were analyzed. The number of lines which fell beyond the boundaries of the 95% confidence intervals of the check variety, Hwaseongbyeo was checked for each quantitative trait, and of those fertility showed the highest variation frequency (85.6%), followed by plant height (77.5%), flag leaf length (66.5%), grains per panicle (42.2%), days to heading (34.5%), panicle length (30.7%) and panicles per hill (22.7%). And the variations of quantitative traits except days to flowering appeared to move in the negative direction compared to "Hwaseongbyeo". Variability within lines was also observed for quantitative and qualitative traits. Twenty-nine $R_2$ lines (7%) segregated for pubescence and 130 $R_2$ lines (30%) showed variation with regard to fertility. This suggests that mutations usually occur before diploidization. Twenty-nine $R_2$ lines representing a wide spectrum of variation were chosen for RAPD analysis. The number of lines showing DNA polymorphism compared to Hwaseongbyeo ranged 0 from to 10 according to the primer used and this seems to indicate that specific loci have highly mutable genomic site.utable genomic site.

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