• Title/Summary/Keyword: LIF system

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Visualization of Combustion by Using Laser Diagnostic Techniques (레이저 진단기법을 이용한 연소 가시화 기술)

  • Chung S. H.;Won S. H.
    • Journal of the Korean Society of Visualization
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    • v.2 no.1
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    • pp.52-56
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    • 2004
  • Several visualization techniques of laser diagnostics are presented for combustion phenomena, including Mie scattering for flow, Rayleigh and Raman scattering spectroscopy for major species, laser-induced fluorescence for minor species, and laser-induced incandescence for soot. These techniques have been applied to understand the various combustion phenomena more clearly, including buoyancy-dominant flow system, diffusion flam oscillation, laminar and turbulent lifted flames, flame propagation along a vortex ring, and soot zone characteristics. The usefulness of laser diagnostics on a better understanding of physical mechanism is demonstrated.

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The Investigation of Cell Culture Conditions to Maintain Chicken Embryonic Stem Cells as Totipotent Cells

  • Du, Lixin;An, Jing
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.8
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    • pp.1102-1107
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    • 2003
  • The ES cell can provide a useful system for studying differentiation and development in vitro and a powerful tool for producing transgenic animalds. To investigate the culture condition of chicken embryonic stem (CES) cells which can retain their multipotentiality or totipotency, three kinds of feeder layer cells, SNL cells, primary mice embryonic fibroblasts (PMEF) cells and primary chicken embryonic fibroblasts (PCEF) cells, were used as the feeder cells in media of DMEM supplemented with leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF) for co-culture with blastoderm cells from stage X embryos of chicken. The alkaline phosphatase (AKP) test, differentiation experiment in vitro and chimeric chicken production were carried out. The results showed that culture on feeder layer of PMEF yielded high quality CES cell colonies. The typical CES cells clone shape revealed as follows: nested aggregation (clone) with clear edge and round surface as well as close arrangement within the clone. Strong alkaline phosphatase (AKP) reactive cells were observed in the fourth passage cells. On the other hand, the fourth passage CES cells could differentiate into various cells in the absence of feeder layer cells and LIF in vitro. The third and fourth passage cells were injected into the subgerminal cavity of recipient embryos at stage X. Of 269 Hailan embryos injected with CES cells of Shouguang Chickens, 8.2% (22/269) survived to hatching, 5 feather chimeras had been produced. This suggests that an effective culture system established in this study can promote the growth of CES cells and maintain them in the state of undifferentiated and development, which lays a solid foundation for the application of CES cells and may provide an alternative tool for genetic modification of chickens.

A Study on the Technique of Fault Classification in Transmission Lines Using a Combined Adaptive Network-Based Fuzzy Inference System (ANFIS를 이용한 송전선로의 고장판별 기법에 관한 연구)

  • Yeo, Sang-Min;Kim, Cheol-Hwan
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.50 no.9
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    • pp.417-423
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    • 2001
  • This paper proposes a technique for fault detection and classification for both LIF(Low Impedance Fault)s and HIF(High Impedance Fault)s using Adaptive Network-based Fuzzy Inference System(ANFIS). The inputs into ANFIS are current signals only based on Root-Mean-Square(RMS) values of 3-phase currents and zero sequence current. The performance of the proposed technique is tested on a typical 154 kV Korean transmission line system under various fault conditions. Test results show that the ANFIS can detect and classily faults including (LIFs and HIFs) accurately within half a cycle.

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Establishment of Embryonic Stem Cells Derived from Rabbit Embryos (토끼수정란으로부터 배아세포의 분리)

  • 강회성;임경순;최화식;신영수;진동일
    • Korean Journal of Animal Reproduction
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    • v.25 no.3
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    • pp.219-225
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    • 2001
  • To establish rabbit Embryonic Stem (ES) cells, rabbit one-cell embryos were collected and cultured in vitro to blastocysts. Blastocysts were co-cultured with mouse embryonic fibroblasts (MEF), rabbit embryonic fibroblasts (REF) or 570 cells expressing LIF (SNL). Although rabbit ES cells were isolated with low efficiencies, total 8 ES cell lines were kept in vitro with normal colony shape. The MEF was the best feeder for rabbit ES cell isolation in regard to growth rate and undifferentiated morphology. The doubling time of rabbit ES cells in MEF was about 84 hours and the undifferentiated morphology was maintained following passing and freezing processes. These rabbit ES cells were differentiated into embryoid body following the culture in the uncoated dishes, indicating that they were undifferentiated stem cells.

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The Study on the Fuel Vapor Distribution of Homogeneous Charge in a DISI Engine with a 6-Hole Fuel Injector (6공 연료분사기를 장착한 DISI 엔진 내 균질급기의 연료증기 분포 특성)

  • Kim, S.S.
    • Journal of Power System Engineering
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    • v.15 no.1
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    • pp.5-10
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    • 2011
  • The spatial fuel vapor distribution of the homogeneous charge by a 6-hole injector was examined in a optically accessed single cylinder direct injection spark ignition(DISI) engine. The effects of in-cylinder charge motion, and fuel injection pressure, and coolant temperature were investigated using a planar LIF (Laser Induced Fluorescence) technique. It was confirmed that the in-cylinder tumble flow played a little more effective role in the spatial fuel vapor distribution than the swirl flow during the compression stroke at 10 mm and 2 mm planes under cylinder head gasket and the increased fuel injection pressure activated spatial distributions of the fuel vapor. In additions, richer mixtures were concentrated around the cylinder wall by the increase of the coolant temperature.

An Experimental Study on the Lift-off Characteristics of the Triple Flame with Concentration Gradient (농도구배가 삼지화염의 부상특성에 미치는 영향에 관한 실험적 연구)

  • Seo, Jeong-Il;Kim, Nam-Il;Oh, Kwang-Chul;Shin, Hyun-Dong
    • 한국연소학회:학술대회논문집
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    • 2004.06a
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    • pp.7-14
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    • 2004
  • The lift-off characteristics of the triple flame have been studied experimentally with various mean velocities and concentration gradients using a multi-slot burner, which can control the concentration gradient and the mean velocity independently, Lift-off height, axial maximum velocity, flame temperature, and some other characteristics were examined for methane and propane flame, It was found that minimum values of the lift-off heights exist at a certain concentration gradient for constant mean velocity, and this result implies that the propagation velocity has a maximum value at this condition, OH radical distribution was measured with LIF method and velocity variation along streamline was measured with PlV system. In addition maximum temperature along streamline was measured with CARS system. The intensity of the diffusion flame affects on the propagation velocity of triple flame in the region of very weak concentration gradient.

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A Study on the Algorithm for Fault Discrimination in Transmission Lines using Neural Network and the Variation of Fault Currents (신경회로망과 고장전류의 변화를 이용한 고장판별 알고리즘에 관한 연구)

  • Yeo, Sang-Min;Kim, Cheol-Hwan
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.49 no.8
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    • pp.405-411
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    • 2000
  • When faults occur in transmission lines, the classification of faults is very important. If the fault is HIF(High Impedance Fault), it cannot be detected or removed by conventional overcurrent relays (OCRs), and results in fire hazards and causes damages in electrical equipment or personal threat. The fast discrimination of fault needs to effective protection and treatment and is important problem for power system protection. This paper propolsed the fault detection and discrimination algorithm for LIFs(Low Impedance Faults) and HIFs(High Impedance Faults). This algorithm uses artificial neural networks and variation of 3-phase maximum currents per period while faults. A double lines-to-ground and line-to-line faults can be detected using Neural Network. Also, the other faults can be detected using the value of variation of maximum current. Test results show that the proposed algorithms discriminate LIFs and HIFs accurately within a half cycle.

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Study on the Fuel Vapor Distribution of the Stratified Charge in a DISI Engine by PLIF Technique (직분식 전기점화 엔진에서 PLIF기법에 의한 성층 혼합기의 분포특성 연구)

  • Kim, S.S.
    • Journal of Power System Engineering
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    • v.12 no.6
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    • pp.64-69
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    • 2008
  • The spatial fuel distribution of the stratified charge of a high pressure 6-hole injector was examined in a single cylinder optical direct injection spark ignition(DISI) engine. The effects of in-cylinder charge motion, and fuel injection pressure, and coolant temperature were investigated using a planar LIF(Laser Induced Fluorescence) technique. It was confirmed that the in-cylinder tumble flow played more effective role in the spatial fuel distribution of the stratified charge than the swirl flow during the compression stroke and the fuel distribution area increased due to the activation of the fuel vaporization by the increase of the coolant temperature. But, the increase of the fuel supplying pressure could not change the pattern of the fuel vapor distribution against the expectation.

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