• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.13-19
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• 2012

In order to provide the basis for developing practical mouse embryonic stem cells (mESCs) culture method, how the endogenous level of self-renewal-stimulating factor genes was altered in the mESCs by different extracellular signaling was investigated in this study. For different extracellular signaling, mESCs were cultured in 2 dimension (D), 3D and integrin-stimulating 3D culture system in the presence or absence of leukemia inhibitory factor (LIF) and transcriptional level of $Lif$, $Bmp4$ and $Wnt3a$ was evaluated in the mESCs cultured in each system. The expression of three genes was significantly increased in 3D system relative to 2D system under LIF-containing condition, while only $Wnt3a$ expression was increased by 3D culture under LIF-free condition. Stimulation of integrin signaling in mESCs within 3D system with exogenous LIF significantly up-regulated transcriptional level of $Bmp4$, but did not induce transcriptional regulation of $Lif$ and $Wnt3a$. In the absence of LIF inside 3D system, the expression of $Lif$ and $Bmp4$ was significantly increased by integrin signaling, while it significantly decreased $Wnt3a$ expression. Finally, the signal from exogenous LIF significantly caused increased expression of $Lif$ in 2D system, decreased expression of $Bmp4$ in both 2D and 3D system, and decreased expression of $Wnt3a$ in integrin-stimulating 3D system. From these results, we identified that endogenous expression level of self-renewal-stimulating factor genes in mESCs could be effectively regulated through artificial and proper manipulation of extracellular signaling. Moreover, synthetic 3D niche stimulating endogenous secretion of self-renewal-stimulating factors will be able to help develop growth factor-free maintenance system of mESCs.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.117-121
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• 2005

In order to monitor the levels and seasonal variations of EDCs, samples of the discharged effluent from sewage & wastewater treatment plants and river waters were collected. The target EDCs including bisphenol A and alkylphenols were determined by Laser-induced fluorescence(LIF) as in-situ monitoring technique. The category of EDCs showed similar fluorescence spectra and nearly equal decay time. This point makes it hard to distinguish each EBCs from the EDCs mixture by LIF and LIF results were expressed only by the total EDCs. However, LIF monitoring results and GC-MS results was comparable. The correlation coefficient between EDCs concentration acquired from GC-MS and fluorescence intensity from LIF was significant. This study supports the feasibility of the application of LIF into EDCs monitoring In aquatic system.

• Proceedings of the KIEE Conference
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• 1997.07c
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• pp.924-926
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• 1997

A high impedance fault(HIF) in a power system could be due to a downed conductor, and is a dangerous situation because the current may be too small to be detected by conventional means. In this paper, HIF(High impedance fault) and LIF(Low impedance fault) detection methods were reviewed. No single defection method can detect all electrical conditions resulting from downed conductor faults, because high impedance fault have arc phenomena, asymmetry and randomness. Neural network are well-suited for solving difficult signal processing and pattern recognition problem. This paper presents the application of artificial neural network(ANN) to detect the HIF and LIF. Test results show that the neural network was able to identify the high impedance fault by real-time operation. Furthermore, neural network was able to discriminate the HIF from the LIF.

• Journal of Soil and Groundwater Environment
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• v.19 no.5
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• pp.18-25
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• 2014

Traditional investigation procedures of soil and groundwater contamination are followed by soil gas sampling, soil sampling, groundwater sampling, establishment of monitoring wells, and groundwater monitoring. It often takes several weeks to obtain the analysis reports, and sometimes, it needs supplemental sampling and analysis to delineate the polluted area. Laser induced fluorescence (LIF) system is designed for the detection of free-phase petroleum pollutants, and it is suitable for on-site real-time site investigation when coupling with a direct push testing tool. Petroleum products always contain polycyclic aromatic hydrocarbon (PAH) compounds possessing fluorescence characteristics that make them detectable through LIF detection. In this study, LIF spectroscopy of 5 major fuel products was conducted to establish the databank of LIF fluorescence characteristic spectra, including gasoline, diesel, jet fuel, marine fuel and low-sulfur fuel. Multivariate statistical tools were also applied to distinguish LIF fluorescence characteristic spectra among the mixtures of selected fuel products. This study successfully demonstrated the feasibility of identifying fuel species based on LIF characteristic fluorescence spectra, also LIF seemed to be uncovered its powerful ability of tracing underground petroleum leakages.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.192-200
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• 2019

Objective: Leukemia inhibitory factor (LIF) binds to a heterodimeric receptor composed of LIF receptor (LIFR) and glycoprotein 130 (GP130) to transmit signals into the cell. LIF plays an important role in reproduction by regulating immune response, decidualization, and implantation in several species. However, the expression of LIF and LIFR in the endometrium throughout the estrous cycle and pregnancy in pigs is not fully understood. Methods: We analyzed the expression of LIF and LIFR in the endometrium on days 0 (estrus), 3, 6, 9, 12, 15, and 18 of the estrous cycle, and days 12, 15, 30, 60, 90, and 114 of pregnancy, in conceptuses on days 12 and 15, and in chorioallantoic tissues on days 30, 60, 90, and 114 of pregnancy in pigs. We also determined the effects of estrogen and progesterone on the expression of LIF and LIFR in endometrial tissues. Results: The expression of LIF increased in the endometrium during the late diestrus phase of the estrous cycle and during mid- to late- pregnancy, while the expression of LIFR increased during early pregnancy. The expression of LIF was induced by increasing doses of estrogen, whereas the expression of LIFR was induced by increasing doses of progesterone. Conclusion: These results indicate that the expression of LIF and its receptor LIFR in the endometrium is regulated in a stage-specific manner during the estrous cycle and pregnancy, suggesting that LIF and its receptor signaling system may play critical roles in regulating endometrial function in pigs.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.242-246
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• 2010

To determine the trajectories and the impact of rain drops on the facade of a tall building, a particle tracking method is employed form steady state simulation of turbulent flow around the building. The simulation is performed for the upper part of the building comprising a detailed louver system. Rain is trapped at relative high rates on the roof and the penthouse, with Local Intensity Factors (LIF's) of the order of 1. The upper parapets and upper floors get a fair amount of wetting with LIF's of the order of 0.6. The wetting decreases downwards reaching values of 0.2 to 0.25 at the level of the louver system.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.463-467
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• 2004

Rosette-type diffusers with four-ports per riser are constructed in relatively shallow water in Korea. However, the trajectorial bending phenomena due to lower-pressure inside the surrounded buoyant jets on the riser was not considered in most models and was not observed without any experimental results. The buoyant jet behavior affected by the bending effect where there have been growing interests need to be verified experimentally and need to be preceded in the analysis of the characteristics of the buoyant jets oil a riser. The hydraulic model experiments have been carried out to investigate the characteristics of the behavior of horizontal buoyant jets discharged from a Rosette-type riser with four ports as well as single port over a certain range of the experimental conditions including initial momentum and initial buoyancy using LIF (Laser Induced Fluorescence) system to obtain concentration fields. The intensity of the fluorescent light in each pixel on the images obtained from LIF system with the tracer of Rhodamine H was converted to the local dye concentration with a set of calibration procedures to account for the non-uniform distribution of light intensity and the attenuation of light energy by water medium. The experimental results shows that the trajectories from Your ports tend to bend more and more to the inner side with the increase of the densimetric Froude number while the buoyant jet from a single port rises up without any bending phenomena. The previous models, VISJET and Seo et al. (2002), do not simulate the trajectories well except the region before the bending section. This study will focus on the analysis of the behavior of the buoyant jets for mainly a Rosette-type riser by conducting hydraulic model experiments using LIF system.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2585-2590
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• 2007

In this paper, a technique of simultaneously measuring the velocity and the temperature in micro-scale flow is proposed. This method uses particle tracking velocimetry (PTV) for measuring the velocity and laser induced fluorescence (LIF) for measuring the temperature. To measure the accurate velocity and temperature, images for PTV and for LIF are separated by using two light sources and a shutter which is synchronized with a camera. By using only one camera, measurement system can be simplified and the error from complicate optical system can be minimized. Error analyses regarding the concentrations of fluorescent dye and particle and the light source fluctuation are also conducted. It is found that the error of the temperature and the velocity highly depends on the concentration of fluorescent particles which are used for PTV. This technique is applied to the simultaneous measurement of the velocity and the temperature in the electrokinetic flow. It is found that the velocity and temperature vary with the electric field strength and the concentration of electrolyte.

• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.21-26
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• 2012

Suspension culture is a useful tool for culturing embryonic stem (ES) cells in large-scale, but the stability of pluripotency and karyotype has to be maintained $in$ $vitro$ for clinical application. Therefore, we investigated whether the chromosomal abnormality of ES cells was induced in suspension culture or not. The ES cells were cultured in suspension as a form of aggregate with or without mouse embryonic fibroblasts (MEFs), and 0 or 1,000 U/ml leukemia inhibitory factor (LIF) was treated to suspended ES cells. After culturing ES cells in suspension, their karyotype, DNA content, and properties of pluripotency and differentiation were evaluated. As a result, the formation of tetraploid ES cell population was significantly increased in suspension culture in which ES cells were co-cultured with both MEFs and LIF. Tetraploid ES cell population was also generated when ES cells were cultured alone in suspension regardless of the existence of LIF. On the other hand, the formation of tetraploid ES cell population was not detected in LIF-free condition, in which MEFs were included. The origin of tetraploid ES cell population was turned out to be E14 ES cells and not MEFs by microsatellite analysis and the basic properties of them were still maintained despite ploidy-conversion to tetraploidy. Furthermore, we identified the ploidy shift from tetraploidy to near-triploidy as tetraploid ES cells were differentiated spontaneously. From these results, we demonstrated that suspension culture system could induce ploidy-conversion generating tetraploid ES cell population. Moreover, optimization of suspension culture system may make possible mass-production of ES cells.

• Journal of Mechanical Science and Technology
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• v.19 no.2
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• pp.716-727
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• 2005

Comprehensive measurements for velocity and temperature fields have been conducted. A Micro PIV 2-color LIF system have been setup to measure the buoyancy driven fields in a 1-mm heated channel with low Grashof-Prandtl numbers [$86]. Fluorescence microscopy is combined with an MPIV system to obtain enough intensity images and clear pictures from nano-scale fluorescence particles. The spatial resolution of the Micro PIV system is $75{\mu}m\;by\;67{\mu}m$ and error due to Brownian motion is estimated $1.05\%$. Temperature measurements have achieved the $4.7\;{\mu}m$ spatial resolution with relatively large data uncertainties the present experiment. The measurement uncertainties have been decreased down to less than ${\pm}1.0^{\circ}C$ when measurement resolution is equivalent to $76\;{\mu}m$. Measured velocity and temperature fields will be compared with numerical results to examine the feasibility of development as a diagnostic technique.