• 대한화학회지
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• 제63권2호
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• pp.85-93
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• 2019

A panel of chalcone-sulphonamide hybrids has been designed by tethering appropriate sulphonamide scaffold with substituted chalcones as a multi-target drug for anticancer screening. Chalcones were prepared by Claisen-Schmidt condensation reaction of a substituted aldehyde with para aminoacetophenone. All the synthesized compounds were evaluated against selected five cancer cell lines, MCF-7 (Breast cancer), DU-145 (Human prostate Carcinoma), HCT-15 (Colon cancer), NCIH-522 (stage 2, adenocarcinoma; non-small cell lung cancer) and HT-3 (Human cervical cancer). Most of the synthesized chalcone-sulphonamide hybrids showed amended cytotoxic activity against various cancer cell lines which may be attributed to the linkage of sulphonamide with chalcone skeleton. The synthesized compounds were characterized by FT-IR, $^1H$ NMR, $^{13}C$ NMR and HR-LCMS and spectral study assert the structures of synthesized sulphonamide-chalcone hybrids.

• 대한화학회지
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• 제54권6호
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• pp.687-695
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• 2010

L이 urea, acetamide, semicrabazide, formamide 인 thiocyanato (L) Cobaloxime 착물을 합성하고 특성을 규명하였다. Thiocyanato(L)cobaloximes (SCNCo$(DH)_2$(L))과 benzyl (aquo) cobaloxime $PhCH_2Co(DH)_2(OH_2)$를 반응시켜 일반 분자식 $PhCH_2Co(DH)_2SCNCo(DH_2)(L)$를 갖는 일련의 thiocyanato로 연결된 dicobaloximes 생성물을 얻었다. Thiocyanato 리간드의 다리결합을 포함하고 있는 dicobaloximes의 조성에 대한 증거로 말단의 thiocyanocobaloxime (SCNCo$(DH)_2$(L))으로부터 dicobaloxime이 형성되면서 vCN이 $20-45cm^{-1}$ 증가하게 되는 적외선 데이터를 들 수 있다. 이러한 두 일련의 물질에 대한 더많은 특성을 ($^1H$, $^{13}C$) NMR, LCMS 및 원소분석을 통하여 확인하였다. Thiocyanato (L) cobaloximes 및 thiocyanato로 연결된 dicobaloxime의 항균 활성은 E. Coli에 의해 조사하였다. 두 단량체와 이합체 모두의 DNA-결합 행동은 분광학적 방법 및 점성도 측정을 통하여 조사하였다. 그 결과 이합체 착물은 calf-thymus DNA와 DNA의 염기쌍에 말단의 벤질 고리를 통해 사이에 끼인 형태로 결합되어 있음을 나타내었다. 단량체 착물은 DNA와 상호작용하지 않는 것으로 관찰되었다.

• 분석과학
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• 제37권1호
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• pp.25-38
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• 2024

The study aimed to investigate a novel approach by utilizing liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) to separate, identify and characterize very nominal quantities of degradation products (DPs) of balsalazide along with its process related impurities without isolation from their reaction mixtures. The impurities along with balsalazide were resolved on spherisorb ODS2 (250×4.6 mm, 5.0 ㎛) column at room temperature using 0.2 M sodium acetate solution at pH 4.5 and methanol in the ratio of 55:45 (v/v) as mobile phase pumped isocratically at 1.0 mL/min as mobile phase and UV detection at 255 nm. The method shows sensitive detection limit of 0.003 ㎍/mL, 0.015 ㎍/mL and 0.009 ㎍/mL respectively for impurity 1, 2 and 3 with calibration curve liner in the range of 50-300 ㎍/mL for balsalazide and 0.05-0.30 for its impurities. The balsalazide pure compound was subjected to stress studies and a total of four degradation products (DPs) were formed during the stress study and all the DPs were characterized with the help of their fragmentation pattern and the masses obtained upon LC-MS/MS. The DPs were identified as 3-({4-[(E)-(4-hydroxyphenyl) diazenyl]benzoyl}amino)propanoic acid (DP 1), 4-[(E)-(4-hydroxyphenyl)diazenyl] benzamide (DP 2), 5-[(E)-(4-carbamoylphenyl)diazenyl]-2-hydroxybenzoic acid (DP 3) and 3-({4-[(E)-phenyldiazenyl]benzoyl}amino)propanoic acid (DP 4). Based on findings, it was concluded that, the proposed method was successfully applicable for routine analysis of balsalazide and its process related impurities in pure drug and formulations and also applicable for identification of known and unknown impurities of balsalazide.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.237-245
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• 2013

Canthaxanthin (cx) is a potent antioxidant that is chemically synthesized at the industrial scale and has imperative applications in the cosmetic and feed industries. An orange pigmented mesophilic bacterium, designated as K44, was isolated from soil samples of Kargil, India. Biochemical tests, 16S rRNA gene sequencing, and FAME analysis of the bacterium indicated it to belong in the genus Dietzia and is distinct from human isolates. The strain showed 98% 16S rRNA gene sequence homology with Dietzia maris DSM 43102. High-performance liquid chromatography profile of the pigments isolated from K44 showed two major peaks absorbing at 465.3 and 475 nm. The liquid chromatography-mass spectrometry (LC-MS) analysis of both these peaks revealed their m/z to be 564. The molecular weights, LC-MS/MS fragmentation patterns, and ${\lambda}_{max}$ of these fractions corresponded to all-trans- (475 nm) and 9-cis-(465.3 nm) cx isomers. The antioxidant activities of cis- and trans-cx isomers isolated from this bacterium were found to differ, where the cis-isomer showed higher free radical, superoxide radical, and reactive oxygen species scavenging activities than the alltrans- isomer, suggesting that 9-cis-cx is more effective as an antioxidant than the all-trans-cx.