• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.167-173
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• 2014

Red yeast rice (RYR) is the product of fermented yeast by Monascus strains on rice, and has recently become a popular dietary supplement as a traditional food in Asia. RYR contains monacolin K substances known to inhibit cholesterol synthesis as efficiently as statin drugs. To determine the optimal rice cultivar for manufacturing RYR, 7 rice cultivars (Goami, Goami2, Sangjuchalbyeo, Seolgaeng, Saegyejinmi, Yeonghojinmi and Chilbo) were fermented using two Monascus strains (M. ruber KCTC6122 and KCCM60141 of M. ruber) in this study. The monacolin K content of Sangjuchalbyeo were 47.24 ppm on KCTC6122 cultures and 117.03 ppm on KCCM60141 cultures, respectively. Other cultivars, especially Goami and Goami2, which had less content of monacolin K could not seem to ferment normally because those didn't show red color. These results imply that Sangjuchalbyeo can be optimal rice cultivar as a commercial RYR which is well fermented rice and has high content of monacolin K.

• Food Science of Animal Resources
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• v.29 no.5
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• pp.619-626
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• 2009

As a trial for the development of a new starter culture for yogurt products, more than two hundred lactic acid bacteria strains were isolated from raw milk and healthy human feces. The strains that showed excellent growth and acid production ability in the 10% skim milk media were selected and identified as Lactobacillus casei through the API carbohydrate fermentation pattern and 16S rDNA sequence analysis. L. casei CU2604 was further investigated for its physiological characteristics as a starter culture compared with a commercial strain. The CU2604 strain showed good acid production and growth characteristics in milk, which were comparable to those of the L. casei Shirota strain. Despite the fact that both these strains displayed the same sugar fermenting pattern and PFGE band pattern, and had similar growth characteristic in milk, L. casei CU2604 exhibited different fatty acid composition in the cell wall, showed more tolerance to bile and to pH, and presented better growth inhibition activity against pathogenic bacteria. Based on these results, the L. casei CU2604 strain holds great promise for use as a novel and efficient starter culture in the production of yogurt. Additional studies on the probiotic characteristics of this strain are currently being conducted.

• Journal of Society of Preventive Korean Medicine
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• v.20 no.2
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• pp.97-109
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• 2016

Objectives : The effects of Jaeumkanghwatang (JEKHT) co-administration on the pharmacokinetics of tamoxifen were observed after oral combination treatment of tamoxifen 50 mg/kg with JEKHT 100 mg/kg on JEKHT 6-day repeated oral pretreated rats with 8-day repeated co-administration to confirm the effects of JEKHT co-administration on the pharmacokinetics of tamoxifen. Methods : Six days after pretreatment of JEKHT 100 mg/kg, tamoxifen 50 mg/kg was co-administered with JEKHT 100 mg/kg, once a day for 8 days within 5 min. The blood were collected at 30 min before administration, 30 min, 1, 2, 3, 4, 6, 8 and 24 hrs after end of first and last 8th tamoxifen treatment, and plasma concentrations of tamoxifen were analyzed using LC-MS/MS methods. PK parameters of tamoxifen ($T_{max}$, $C_{max}$, AUC, $t_{1/2}$ and $MRT_{inf}$) were analysis as compared with tamoxifen single administered. Results : Six-day repeated oral pretreatment of JEKHT and 8-day repeated oral co-administration of tamoxifen within 5 min did not influenced on the plasma concentrations and pharmacokinetic parameters of tamoxifen, oral bioavailability, as compared with tamoxifen single treated rats, except for some negligible effects. Conclusions : It is concluded that JEKHT did not influenced on the plasma concentrations and pharmacokinetic parameters, the oral bioavailability of tamoxifen. Therefore, it is considered that co-administration of JEKHT and tamoxifen will be provide an effective novel treatment regimen on the comprehensive and integrative medicine for breast cancer patients, if they showed favorable synergic effects on the pharmacodynamics or reduce the tamoxifen treatment related toxicity and side effects in future studies.

• Journal of Society of Preventive Korean Medicine
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• v.17 no.2
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• pp.139-155
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• 2013

Purpose : This study was aim to evaluate effects of pharmacodynamics and toxicity in combination therapy of donepezil with Gongjindan. The effects of Gongjindan co-administration on the pharmacokinetics (PK) of donepezil were observed after single and 7-day repeated oral co-administration with 1.5hr-intervals, to evaluate synergic pharmacodynamics and reduce toxicity of combination therapy of donepezil with Gongjindan. Materials and Methods : After 10mg/kg of donepezil treatment, Gongjindan100mg/kg was administered with 1.5hr-intervals. The plasma were collected at 30min before administration, 30min, 1, 2, 3, 4, 6, 8 and 24hrs after end of first and last 7th donepezil treatment, and plasma concentrations of donepezil were analyzed using LC-MS/MS methods. Results : Gongjindan markedly inhibited the absorption of donepezilregardless of sample time, from 30min to 8hrs after end of first 1.5hr-interval co-administration as compared with donepezil single treated rats. Especially the absorption of donepezil was significantly decreased at 2, 4, 6 and 8hrs after co-administration as compared with donepezilsingle treated rats. Accordingly, the Cmax (-26.236%), $AUC_{0-t}$(-26.02%) and $AUC_{0-inf}$(-25.90%) of donepezil in 1.5hr-interval co-administered rats were dramatically decreased as compared with donepezilsingle treated rats, respectively. However, no meaningful changes on the plasma donepezil concentrations and pharmacokinetic parameters were detected after end of last 7th 1.5hr-interval co-administration as compared with donerezil single treated rats, except for non-significant slight increases of Tmax(16.67%) detected in co-administered rats as compared with donepezil single treated rats. Conclusion : These findings are considered as direct evidences that Gongjindan also decreased oral bioavailability of donerezil as inhibited the absorptions, when they were co-administered with 1.5hr-intervals, but they may be adapted after 7 days continuous repeated l.5hr-interval co-administration.

• The Korean Journal of Pesticide Science
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• v.12 no.4
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• pp.315-322
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• 2008

In this study, a simple, effective, and sensitive method has been developed for the quantitative residue analysis of cyhalofop-butyl and its metabolite cyhalofop acid in water and soil when kept under laboratory conditions. The content of cyholofop-butyl and cyhalofop acid in water and soil was analyzed by first purifying the compounds through liquid-liquid extraction and partitioning followed by Silica gel (adsorption) chromatography. Upon the completion of the purification step the residual levels were monitored through high-performance liquid chromatography (HPLC) using a UV absorbance detector. The recoveries of cyhalofop-butyl from three replicates spiked at two different concentrations ranged from 82.5 to 100.0% and from 66.7 to 97.9% in water and soil, respectively. The limit of detection and minimum detection level of cyhalofop-butyl in water and soil was 0.02 ppm and 10 ng, respectively. The recoveries of cyhalofop acid ranged from 80.7 to 104.8% in water and from 76.9 to 98.1 % in soil. The limit of detection of cyhalofop acid was 0.005 ppm in water and 0.01 ppm in soil, while the minimum detection level was 2 ng both in water and soil. The half-live of cyhalofop-butyl was 4.14 and 6.6 days in water and soil, respectively. The method was successfully applied to evaluate cyhalofop-butyl residues in water and soil applied aj. 30% emulsion, oil in water (EW) product.

• Korean Journal of Food Science and Technology
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• v.43 no.5
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• pp.553-557
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• 2011

Garlic specific organic compounds were separated and purified using a recycling preparative high-performance liquid chromatography (HPLC) from blanched garlic cloves. Identification of the compounds involved comparing the previously reported HPLC retention times as well as other identification methods including $^1H$- and $^{13}C$-nuclear magnetic resonance and liquid chromatography-mass spectrometry. The yields of garlic specific organic compounds were 12.2, 42.5, 1.6, 1.2, and 4.8% on wet weight basis of garlic for alliin(S-allyl-L-cysteine sulfoxide), isoalliin(S-1-propenyl-L-cysteine sulfoxide), ${\gamma}$-glutamyl-S-allylcysteine, ${\gamma}$-glutamyl-S-1-propenylcysteine and ${\gamma}$-glutamyl-phenylalanine, respectively. All the compounds, except for ${\gamma}$-glutamylphenylalanine, contained sulfur.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.54-54
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• 2018

숨은물뱅듸 산림유전자원 보호구역은 해발 950 m 이상 지역의 습지로서 오름으로 둘러싸인 넓은 웅덩이 형태로 환경부 멸종위기 2급 야생식물인 자주땅귀개를 비롯한 다양한 습지 식물과 이를 둘러싸고 있는 산림지역을 포함하여 산림청에서는 산림유전자원 보호구역으로 지정 관리되고 있다. 본 조사는 숨은물뱅듸 산림유전자원 보호구역을 대상으로 식물상을 조사하여 식물종 다양성을 보존하기 위한 기초자료를 만들기 위해 실시하였다. 본 연구는 2018년 7월 24일부터 8월 28일까지 총 4회에 걸쳐 현장조사를 통하여 표본을 채집하고 기록하여 정리하였다. 그 결과, 숨은물뱅듸에 자생중인 식물은 양치식물 8과 11속 17종 17분류군, 나자식물 2과 2속 2종 2분류군, 피자식물 56과 121속 167종 5변종 1품종 173분류군 총66과 134속 186종 5변종 1품종의 총 192분류군이 조사되었다. 이 중 환경부 멸종위기야생식물은 자주땅귀개 1종이 확인되었고, 제주특산식물 6분류군, 한국특산식물 2분류군이 확인되었다. 식물구계학적특정식물은 총37분류군이며 V등급 5분류군, IV등급 5분류군, III등급 12분류군, II등급 5분류군, I 등급 10분류군이 확인되었다. 한국의 적색목록 식물은 위기(EN) 1분류군, 취약(VU) 1분류군, 준위협(NT) 1분류군, 관심대상(LC) 6분류군, 미평가(NE) 3분류군으로 나타났다. 조사된 식물들 대상으로 생활형을 분석해보면, 휴면형은 Ch 47분류군으로 가장 많이 나타났고, G(30분류군), MM(24분류군), HH(23분류군) 순으로 나타났다. 번식형은 R5가 101분류군, 산포기관형은 D4가 84분류군, 생육형은 e가 89분류군으로 가장 많이 나타났다. 반면, 외래식물 1분류군이 출현한 것으로 보아 숨은물뱅되는 아직까지 보전이 잘 되어 있고, 식물종다양성이 우수하며 식물학적으로 가치가 매우 높은 것으로 판단되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.61-61
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• 2018

한경 곶자왈 산림유전자원보호구역은 개가시나무와 백서향 등을 포함한 암괴지대의 상록활엽수림으로서 그 중요성이 인정되어 산림유전자원 생태보전지역으로 지정 관리되고 있다. 따라서 본 지역의 식물상을 조사하여 곶자왈의 분포식물을 파악하고자 실시하였다. 본 연구는 한라산 서쪽 곶자왈지역 중 곶자왈 산림유전자원 보호구역으로 지정된 지역을 2017년 6월부터 2017년 9월까지 20여 차례 현장조사를 통하여 표본을 수집하고 이를 기록 정리하였다. 조사지역의 식물은 총 83과 181속 236종 23변종 2품종 총 261종으로 조사결과 양치식물이 7과 18속 30종 2변종 32분류군, 나자식물이 1과 1속 2분류군, 피자식물은 74과 162속 204종 21변종 2품종 227분류군 이었다. 그 중 쌍자엽식물은 67과 139속 179종 16변종 1품종 196분류군이고, 단자엽식물이 7과 23속 25종 5변종 1품종 31분류군이었다. 조사에서 확인된 환경부 멸종위기야생식물은 개가시나무 1종 이었다. 식물 구계학적 특정식물로는 총 97분류군이 조사되었다. 등급별로는 V등급 검정개관중, 개가시나무로 2분류군, IV등급 백서향, 녹나무 등 12분류군, III등급 아왜나무, 호자나무 등 37분류군, II등급 올벚나무, 개산초 등 13분류군, I 등급 푸조나무, 자금우 등 33분류군으로 확인되었다. 한국의 적색목록 식물을 분석해보면 취약(VU)은 개가시나무, 섬다래 등 3분류군, 준위협(NT)은 백서향, 약난초로 2분류군, 관심대상종(LC)은 골고사리, 새우난초 등 9분류군, 미평가종(NE)은 빌레나무 1분류군으로 나타났다. 이 결과는 청수 저지 곶자왈 지역의 생태학적 자료로 제주도 내 곶자왈과 비교하였을 때 맹아가 발달한 종가시나무가 우점하는 지역으로 향후 상록수림 천이에 의해 상록수 아래에 자라는 식물로 낙엽활엽수가 대체될 것으로 추정되어 지속적인 조사가 요구된다.

• The korean journal of orthodontics
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• v.25 no.5 s.52
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• pp.605-611
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• 1995

The purpose of this study was to evaluate effects of time on shear bond strengths of a light-cured glass ionomer cement and chemically cured resin cement to enamel, and to observe the failure patterns of bracket bondings. Shear bond strength of a light-cured glass ionomer cement were compared with that of a resin cement. Metal brackets were bonded on the extracted human bicuspids. Specimens were subjected to a shear load(in an Instron machine) after storage at room temperature for 5 and 15 minutes; after storage in distilled water at $37^{\circ}C$ for 1 or 35 days. The deboned specimens were measured In respect of adhesive remnant index. The data were evaluated and tested by ANOVA, Duncan's multiple range test, and t-test, and those results were as follows. 1. The shear bond strength of light-cured glass ionomer cement is higher than that of resin cement at 5 and 15 minutes. 2. The shear bond strengths of both light-cured glass ionomer cement and resin cement increase with time. There was no significant difference in those of both 1 day group and 35 day group 3. Light-cured glass ionomer cement is suitable as orthodontic bracket adhesives

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.3023-3027
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• 2015

Purpose: To investigate the effects of epidermal growth factor receptor (EGFR), cytokeratin 19 (CK19), cytokeratin 20 (CK20) and survinin gene expression on local control (LC) and overall survival (OS) in patients with locally advanced head and neck cancer (LAHNC) who were administered radiotherapy (RT). Materials and Methods: Twenty-six patients who were admitted to Uludag University Medical Faculty Department of Radiation Oncology with a diagnosis of LAHNC (GIII-GIV) were included in this study. Gene expression was evaluated in tumor tissues and peripheral blood. RNA isolation was performed on paraffinized tumor tissues and peripheral blood samples obtained before RT (BR). The densities of the obtained RNAs were analyzed at 260/280 nm. cDNA samples obtained from total RNA,EGFR, CK19, CK20 and survinin gene expression levels were assessed via the Sybr Green method and data were analyzed with the ${\Delta}{\Delta}Ct$ method. The same process was repeated for peripheral blood samples taken after RT (AR). Results: The female/male ratio was 3:23 and the mean age was 56.5 years (38-75years). After radiotherapy, CK19 and CK20 levels in the peripheral blood were found to be correlated according to Pearson correlation analysis(p=0.049). This result indicates a possibility of remaining positive for CK19 and CK20 in the peripheral blood even after RT in patients with CK19, CK20, and EGFR positive tumors before RT. There was a statistically significant correlation between survinin levels measured BR and AR (p=0.028). Conclusions: In this study, we found that patients with any EGFR, CK19, CK20 or survinin positivity in their peripheral blood obtain less benefit from radiotherapy. A wider patient population and advanced protein analyses are necessary in order to increase the reliability of our findings.