• Title/Summary/Keyword: LC3

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Analysis of Photodegradation Products of Organic Photochromes by LC/MS

  • Lim, Young-Hee;Youn, Yeu Young;Kim, Kyung Hoon;Cho, Hye-Sung
    • Mass Spectrometry Letters
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    • v.3 no.4
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    • pp.101-103
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    • 2012
  • The ultraviolet (UV) degradation products of photochromic naphthoxazine and naphthopyran derivatives in acetonitrile were separated and identified using liquid chromatography-mass spectrometry (LC-MS). Photodegradation resulted in oxidation of products.

Pollination Methods for Overcoming Pre-fertilization Incompatibility in Interspecific Crosses between Lilium longiflorum 'Gelria' and L. cernuum Native in Korea (나팔나리와 자생 솔나리와의 종간 교잡 시 수정 전 불화합성 타파를 위한 수분방법)

  • Kim, Young Jin;Park, Sung Min;Kim, Jong Hwa
    • Horticultural Science & Technology
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    • v.19 no.3
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    • pp.373-377
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    • 2001
  • This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

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Formation of Provitamin-$B_5$ Liquid Crystal with Hydrogenated Lecithin and Its Effectiveness of Moisturizing Activity (HL에 의한 프로비타민-$B_5$ 액정의 형성과 보습효과)

  • Kim, In-Young;Zhoh, Choon-Koo;Ryoo, Hee-Chang
    • Journal of the Korean Applied Science and Technology
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    • v.20 no.2
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    • pp.101-109
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    • 2003
  • Provitamin $B_5$ liquid crystal ($PVB_5$-LC) was the new emulsion system to enhance moisturizing activity on the skin. In this study, it should be mentioned that PVB5-LC could be prepared with the main compound of hydrogenated lecithin (HL) in oil-in water (O/W) emulsion. The key ingredient of humectants was contained 2% of provitamin $B_5$ ($PVB_5$) into the $PVB_5$-LC. The best suitable compositions of $PVB_5$-LC were made from 4.0 wt% of HL, 4.0 wt% of cetostearyl alcohol (CSA) as emulsifier and gelling agent, 3.0 wt% of 1,3-butylene glycol (1,3-BG) and 2.0 wt% of glycerin as moisturizers, 3.0 wt% of cyclomethicone (CMC), 3.0 wt% of isononanoate (ININ), 3.0 wt% of capric/caprylic triglyceride (CCTG), 3.0 wt% of macadamia nut oil (MNO) as emollients. As the analytical result of $PVB_5$-LC, it could know that the distribution range of particle size was 0.14 to 12.37 m level (mean size 3.24 m). It was certified the multi lamellar phase around the droplet of liquid crystal when observed the droplet particles through a polarization microscope. And it clinically was tested the effectiveness of moisturizing activity (in-vivo) compared with control sample (O/W emulsion). The effectiveness of moisturizing activity of $PVB_5$-LC with Skincon-200EX after 6 hours went up 49.0% (p<0.05, n=20) whereas the effectiveness of moisturizing activity of generally O/W emulsion cream was 25.7% (p<005, n=20). Also, in case of Comeometer CM-825, the moisturizing activity of $PVB_5$-LC after 6 hours rose 36.6% (p<0.05, n=20) whereas the effectiveness of moisturizing activity of generally O/W emulsion cream was 10.8%. Therefore, it was known that the effectiveness moisturizing activity of $PVB_5$-LC with HL was remarkably superior compared with O/W emulsion cream.

A Study on Wideband LC Oscillator Using Fat Dipole Antenna (팻 다이폴 안테나를 이용한 광대역 LC 오실레이터에 관한 연구)

  • Lee, Sang-Heun;Yoon, Young-Joong;Heo, Hoon;Nam, Sang-Hoon;Lee, Woo-Sang;Choi, Do-Won
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.11
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    • pp.1185-1192
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    • 2008
  • In this paper, a wideband LC oscillator which is composed of a high voltage switch and a fat dipole antenna was designed and radiation characteristics are analyzed. A Marx generator was used as a high voltage pulse generator of the proposed wideband LC oscillator and $90^{\circ}$ corner reflector was used to obtain high directivity. According to simulated and measured results, 3 dB bandwidth of high voltage switch without fat dipole based on the received power is about 9%(${\lambda}_1=0.7\;m$) and bandwidth Is about 30%(${\lambda}_2=1\;m$) by using the LC oscillator containing high voltage switch and fat dipole. Consequently, fat dipole affects not only radiating power but also operating frequency and bandwidth of the LC oscillator. This study will be useful to determine operating frequency and radiating power when we design LC oscillator which uses a high voltage switch and a fat dipole.

Effects of Ammina on Survival and Growth of the Flounder Larva, Paralichthys olivaceus (넙치, Paralichthys olivaceus 자어의 생존과 성장에 미치는 암모니아의 영향)

  • KIM Hyung-Soo;KIM Heung-Yun;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.488-495
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    • 1997
  • The effects of ammonia on survival and growth of the flounder larva, Paralichthys olivaceus, were examined by a static renewal bioassay method. The $96\;hr-LC_{50}$ with the developmental stages during the period from 1day to 23 day-old larvae ranged 0.273 to 1.023 mg $NH_3/\ell$. Tolerance of the larvae to ammonia toxicity was much sensitive at the early larval stage, and increased with the growth of the larvae. Threshold $96hr-LC_{50}$ in 1, 3 and S day-old larvae after hatching were 0.293, 0.248 and 0.379 mg $NH_3/\ell$, respectively. Survival rate and growth in body weight and body weight of the larva were reduced with increase of ammonia concentration in the range of 0.055 and 0.341 mg $NH_3/\ell$. The no-observable-effect concentration (NOEC) and lowest-observable- effect concentration (LOEC) of the flounder larve were 0.102 and 0.174 mg $NH_3/\ell$ for body length, and 0.151 and 0.198 mg $NH_3/\ell$ for body weight, respectively. Chronic value (ChV), which is the geometric mean of the NOEC and $NH_3/\ell$ to body length of the larvae were 0.124 mg $NH_3/\ell$. The coefficient of variation (CV) for body length was higher at high concentration than at low concentration.

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Stability and Cytotoxicity of Fab-Ricin A Immunotoxins Prepared with Water Soluble Long Chain Heterobifunctional Crosslinking Agents

  • Woo, Byung-Ho;Lee, Jung-Tae;Park, Myung-Ok;Lee, Kang-Ro;Han, Jeung-Whan;Park, Eun-Seok;Yoo, Sun-Dong;Lee, Kang-Choon
    • Archives of Pharmacal Research
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    • v.22 no.5
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    • pp.459-463
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    • 1999
  • The effects of the hindered and non-hindered water soluble long-chain disulfide bonds on the stability and cytotoxicity of the ricin A chain (RTA) immunotoxin were examined. The RTA immunotoxins were prepared with the Fab fragments of anti-common acute lymphoblastic leukemia antigen (CALLA) monoclonal antibody (Fab-RTA) using sulfosuccinimidyl-6-[(-methyl-(-2-pyridyldithio)toluamido]toluamido]hexanoate (S-LC-SMPT) and sulfosuccinimidyl-6-[3-(2-pyridyldithio-propionamido]hexanoate (S-LC-SPDP). The prepared Fab-RTA immunotoxins were evaluated for their conjugation yield, immunoreactivity, thermal and disulfide bond stability and cytotoxicity. The conjugation yield of the Fab-RTA immunotoxin from the water soluble long chain crosslinking agents, S-LC-SMPT and S-LC-SPDP, were comparable. Both Fab-RTA immunotoxins exhibited a similar immunoreactivity and thermal stability in aqueous solution. However, S-LC-SMPT -mediated Fab-RTA, sterically hindered, showed an enhanced disulfide bond stability in vitro over S-LC-SPDP mediated one. In the cytotoxicity against antigenic cell Daudi, the S-LC-SMPT -mediated RTA immunotoxin maintained a comparable cytotoxicity, compared with S-LC-SPDP mediated Fab-RTA immunotoxin.

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COMPARATIVE STUDY ON THE FLUORIDE RELEASE AND COMPRESSIVE STRENGTH OF SEVERAL F-CONTAINING RESTORATIVE MATERIALS (수종의 불소 함유 수복재의 불소 유리 및 압축 강도에 관한 연구)

  • Park, Jee-Young;Kim, Jong-Soo;Kim, Seung-Oh
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.3
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    • pp.469-476
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    • 2008
  • This study was performed to compare the fluoride release pattern and compressive strength of recently developed resin-modified glass ionomers($Ketac^{TM}$ N 100 and Fuji Fil LC) with those of conventional glass ionomer restorative material(Fuji II LC). Fifteen sample discs(6 mm diameter and 1 mm height) were prepared for each tested material. The fluoride release was measured by pH/ISE meter(750P, Istek, Korea) for 31 days. For compressive strength experiment, fifteen cylindrical specimens were prepared for each tested material. Each specimen was submitted to compressive strength testing in an universal testing machine(Kyung-sung Testing Machine Co., Korea) at crosshead speed of 5.0mm/min until failure. The results can be summarized as follows; 1. Fuji Fil LC released the highest amount of fluoride, followed by Fuji II LC and $Ketac^{TM}$ N 100(p<0.05). 2. The compressive strength of Fuji Fil LC was the lowest(p<0.05). However, no significant difference was found from Fuji II LC and $Ketac^{TM}$ N 100(p>0.05). By considering the above results, careful case selection and accurate clinical application is recommended when using $Ketac^{TM}$ N 100 and Fuji Fil LC.

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Physiological Characteristics and Production of Vitamin K2 by Lactobacillus fermentum LC272 Isolated from Raw Milk

  • Lim, Sang-Dong;Kim, Kee-Sung;Do, Jeong-Ryong
    • Food Science of Animal Resources
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    • v.31 no.4
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    • pp.513-520
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    • 2011
  • In order to develop a new starter culture for fermented milk, Lactobacillus fermentum LC272 was isolated from raw milk and its physiological characteristics were investigated. The vitamin $K_2$ concentration of L. fermentum LC272 was $184.94{\mu}g/L$ in Rogosa medium and $63.93{\mu}g/L$ in the reconstituted skim milk. The optimum growth temperature for L. fermentum LC272 was determined to be $40^{\circ}C$ and it took 24 h for the pH to reach 5.2 under this condition. L. fermentum LC272 was more sensitive to rifampicin relative of the other 15 different antibiotics tested, and showed most resistance to streptomycin. L. fermentum LC272 showed higher activities to leucine arylamidase and acid phosphatase. It was comparatively tolerant to bile juice and acid and displayed high resistance against Salmonella Typhimurium and Staphylococcus aureus with rates of 82.9 and 86.3% respectively. These results demonstrated that L. fermentum LC272 could be an excellent starter culture for fermented milk with high levels of vitamin $K_2$ production.

Susceptibility of Medically Important Mosquito Larvae and Larvivorous Fishes to Abate and Abate-S in Korea (국내 주요 모기유충과 천적어류의 Abate 와 Abate-S 에 대한 감수성)

  • 이동규;유효석
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.165-169
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    • 1999
  • A study was performed on the susceptibility of Abate@ and Abate-S@ against 3rd instar larvae of 5 species of mosquitoes including Anopheles sinensis, Aedes dorsalis, Culex inatomii, Cx. pipiens pallens, Cx. tritaeniorhynchus and mosquito larvivorous fishes such as Chinese muddy loaches (Misgurnus muzolepis) and crucian carps (Carassius carassi~4s) in September, 1998. Cx. pipiens larvae showed that the LC5os of both Abatea and Abate-Sa were 0.006 ppm. The LC95 values of this mosquito species appeared 0.070 pprn of Abate@ and 0.035 pprn of Abate-S@ which were more susceptible than the dosage (1.0 ppm) recommended from WHO. An. sinensis larvae appeared 0.009 pprn of LC50 and 0.025 pprn of LCgs. The larvae of Cx. inatomii and Ae. dorsalis showed the most highly susceptible to Abate-S@ among the 5 mosquito species with LC50 values of 1.9 x pprn and 1.1 x pprn respectively, and the LC95 values were 5.2 x 10-hppm and 1.4 x ppm, respectively. On the other hand, Cx. tritaeniorhynchus appeared least affected by Abate-Sa. and was brought 0.048 pprn of LC50 and 0.808 pprn of LCg5. These two insecticides were much less toxic to the fishes than mosquito larvae showing the LC, value of Chinese muddy loaches at 24.145 pprn of Abate and 10.750ppm of Abate-S@. This fish species showed that the LCsos of Abate@ and Abate-S@ were 27.567 pprn and 14.775 ppm, respectively. In case of crucian carps, their LC, values were 7.914 pprn of Abate@ and 6.480 pprn of Abate-S@. Therefore, the maximum safe dosages of the insecticides to the fishes were 8 times as high as the values of LCg5 of the mosquito larvae. This study suggests that Abate@ and Abate-S@ demonstrated highly potential insecticides for further larger scale operational integrated control in some proper aquatic places if other aquatic invertebrates are also safe to the insecticides.

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Marine-derived Ca-Mg complex influences lipid and glucose metabolism, serum metabolites, colostrum profile, and stress hormone in sows over four-parity periods

  • Sungbo Cho;Santi Devi Upadhaya;Woo Jeong Seok;Seyoung Mun;Haeun Lee;Rudolf H. van der Veen;Kyudong Han;In Ho Kim
    • Journal of Animal Science and Technology
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    • v.65 no.6
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    • pp.1308-1322
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    • 2023
  • Minerals is required small amounts among various nutrients, but it has a significant impact on sow longevity and reproduction performance. This study was carried out to see the beneficial effects of marine-derived Ca-Mg complex on the reproductive performance of sows during four-parity periods. Seventy-two gilts ([Yorkshire × Landrace] × Duroc), with an average body weight of 181 kg, were randomly allocated to three groups; CON (basal diet), 0.3LC (CON - MgO - 0.3% limestone + 0.4% Ca-Mg complex), and 0.7LC (CON - MgO - 0.7% limestone + 0.4% Ca-Mg complex). During parity 3 and 4, the expression level of SCD gene was lower in the umbilical cord of piglets born to 0.3LC and 0.7LC sows compared with the CON sows. During parity 2, 3 and 4, SLC2A2 and FABP4 gene expressions were higher in the umbilical cord of piglets born to 0.7LC sows and the placenta of sows from 0.3LC groups, respectively. Ca-Mg complex increased (p < 0.05) Ca and Mg concentrations in sows and their piglets' serum as well as in colostrum regardless of parities. The serum vitamin D concentration was higher (p < 0.05) in their first parity, whereas serum prolactin and estrogen concentrations were higher (p < 0.05) during the fourth and third parity, respectively. The growth hormone concentrations were higher (p < 0.05) in the piglets born to sows during the first and second parity. The fat and immunoglobulin A (IgA) concentrations in colostrum were higher (p < 0.05) during the third and fourth parity, respectively. A reduction (p < 0.05) in salivary cortisol, epinephrine, and norepinephrine concentrations was observed in 0.3LC and 0.7LC sow groups compared with CON after farrowing regardless of parity, however before farrowing, a reduction in norepinephrine was observed. Before farrowing, the epinephrine and norepinephrine concentrations were higher (p < 0.05) during the first and second parity. After farrowing, the concentration of these hormones was higher during the second parity. Taken together, sows' parity and dietary Ca-Mg complex supplementation influenced serum metabolites, colostrum nutrients, stress hormones as well as the gene expressions related to lipid and glucose metabolism.