• Journal of Microbiology and Biotechnology
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• 제5권1호
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• pp.18-21
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• 1995

Endoglucanase gene of Pseudomonas sp. LBC505 was previously cloned in pUCl9 to yield plasmid pLC1. overproduction of endoglucanase was attempted by following ways. First, the endoglucanase gene of Pseudomonas sp. LBC505 cloned in pUCl9(pLC1) was tandemly inserted, step by step, into a expression vector pKK223-3 in a directly repeated form to enhance productivity of endoglucanase. Escherichia coli containing pKCC30 among the resulting plasm ids showed the higher yield of the endoglucanase. Ecoli harboring pKCC30 which had three inserted endoglucanase genes expressed about 12.3 times as much CMCase activity as Ecoli harboring pLCl. Second, the endoglucanase gene was subcloned into Bacillus subtilis expression vector pgnt41 for both overproduction and extracellular secretion of the endoglucanase. A resulting plasmid pgntc15 in Bacillus subtilis expressed 4.3-fold higher levels of CMCase activity than that of E.coli harboring pLCl and the endoglucanase produced was entirely secreted into the culture medium.

• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2008년도 학술대회 1부
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• pp.218-223
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• 2008

본 논문에서는 IP 네트워크에서 동작하는 오디오 협업 시스템의 성능을 향상시키는 방법들을 구현하고 구현된 방법들의 성능을 검증한다. IP 네트워크가 갖는 지연(jitter) 및 패킷 손실 등의 특성은 음성 및 오디오의 품질을 저하시킨다. 이러한 품질 저하를 줄이기 위해, 패킷 손실률은 패킷 크기를 줄임으로써 감소시킬 수 있다는 점에 착안하여 MPEG-2 AAC 오디오 코덱을 이용한 저전송률 오디오 전송 방법을 제안한다. 또한, 음성 전송 시 패킷손실에 따른 음성 품질을 향상시키기 위해 패킷 손실에 견고한 iLBC(Internet Low-Bitrate Codec) 음성 코덱 및 ITU-T G.711 패킷 손실 은닉 알고리즘을 오디오 협업 시스템에 적용한다. 제안한 방법들을 RAT (Robust-Audio Tool)[7]를 기반으로 구성된 오디오 협업 시스템에 구현하였다. 구현한 결과, 256 kbit/s에서 동작하는 MPEG-2 AAC 오디오 코덱은 압축하지 않고 전송된 오디오와 유사한 품질을 제공하는 것을, iLBC와 G.711 패킷 손실 은닉 알고리즘은 2~10% 패킷 손실 환경에서 음질을 개선하는 것을 확인할 수 있었다.

• 한국미생물·생명공학회지
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• 제18권6호
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• pp.633-639
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• 1990

Cellulase 복합체와 xylanase를 동시에 분비하는 Pseudomonas sp. LBC 505와 CYC 10의 cellulase 유전자를 pUC19를 사용하여 E.coli에 클로닝시켰다. Congo red 염색시 노란색 환을 형성하는 대장균 형질전환에서 7.0Kb-와 4.6Kb-HindIII 단편을 함유한 재조합 플라스미드 pLC1과 pLC2를 가각 분리하였다. DNA hybridization 실험에서 pLC1 과 pLC2는 Pseudomonas sp. LBC 505와 CYC 10 유래임이 각각 밝혀졌고, Immunoassay 실험에서도 유사성이 인정되었다. pLC1을 함유하고 있는 대장균은 cellulas의 24를 세포외로 분비하였고, 효소활성은 모균주에 비해 1.4배 증가하였다. pLC1과 pLC2의 효소학적 성질도 모균주와 동일하였으며, 기질특이성과 HPLC로 유리당을 분석한 결과, 클로닝된 유전자는 endo type인 것으로 나타났다.

• International Journal of Precision Engineering and Manufacturing
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• 제8권4호
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• pp.10-15
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• 2007

Taguchi methods have been used for a long time to improve the product quality and process performance of a manufacturing system, Few researchers have applied this methodology in laser beam cutting (LBC) of sheet metals and found the considerable improvement in cut qualities. In all experimental investigations of LBC so far, the objective was to optimize the single quality characteristic at a time. In this paper the simultaneous optimization of multiple quality characteristics such as Kerf width and material removal rate (MRR) during pulsed Nd:YAG LBC of thin sheet of magnetic material (high Silicon-steel) has been presented using Taguchi's quality loss function. The results show the considerable improvement in multiple S/N ratio as compared to initial cutting condition. Also, the comparison of results from single and multi-objective optimization have been presented and it was found that the loss in quality is always possible shifting from single quality to multiple quality optimization.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2016년도 추계학술대회 논문집
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• pp.33-34
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• 2016

This paper presents a load sharing method based on the low bandwidth communication (LBC) applied to a DC microgrid in order to balance the state of charge (SOC) of the battery units connected in parallel to the common bus. In this method, SOC of each battery unit is transferred to each other through LBC to calculate average SOC value. After that, droop coefficients of battery units are adjusted according to the difference between SOC of each unit and average SOC value of all batteries in the system. The proposed method can effectively balance the SOC of battery units in charging and discharging duration with a simple low bandwidth communication system.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2010년도 제39회 하계학술대회 초록집
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• pp.325-325
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• 2010

최근 결정질 실리콘 태양전지 분야에서는 태양전지의 Voc와 Isc의 증가를 통한 효율 향상을 목적으로 후면 passivation에 대한 연구가 활발하게 진행되고 있다. Local-Back Contact은 최적화된 후면 passivation 박막을 이용한 태양전지 제조방법이다. 고효율 태양전지 개발을 위해 최적의 laser 가공 조건이 확립되어야 한다. 본 연구에서는 고효율의 LBC 태양전지 개발을 위해 ONO 구조의 후면 passivation 박막에 laser ablation 조건을 가변하여 LBC 태양전지를 제작하고 그 특성을 분석하였다. 본 연구에 사용된 laser는 355nm 파장을 갖는 UV laser를 사용하였다. laser 파워는 5W, 주파수는 30kHz로 하였을 때 폭 20um, 깊이 5um의 홀을 형성시킬 수 있었다. 후면 접촉 면적의 영향을 확인하기 위하여 laser ablation 간격을 300um, 500um, 700um으로 가변하여 공정을 진행하였다. 태양전지 제조 결과 spacing 300um일 경우 효율이 높게 측정되었으며, laser ablation의 데미지를 줄이기 위한 FGA 처리시 웨이퍼 표면의 데미지를 줄여 carrier lifetime 향상에 기여하는 것을 확인할 수 있었다. 본 연구의 결과를 이용하여 향후 후면 passivation 극대화 및 접촉면적 가변을 통한 고효율 LBC 태양전지 개발이 가능할 것으로 판단된다.

• 대한세포병리학회지
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• 제18권1호
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• pp.20-28
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• 2007

This study was performed to compare Surepath$^{TM}$ liquid-based smear and a conventional cervicovaginal smear with reference to a histological diagnosis. A hybrid capture test (HCII) was also performed and analyzed. We collected matched cases for cervicovaginal cytology-histology: 207 cases for conventional cytology (CC) and 199 cases for liquid-based cytology (LBC). HCII was performed in 254 patients. When a cytological diagnosis of ASCUS or above (ASCUS+) is classified as positive and a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 91.7% and 75.9%, respectively and the sensitivity and specificity for CC was 62.6% and 96.1%, respectively. When a cytological and histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for LBC was 77.5 and 96.6%, respectively and the sensitivity and specificity for CC was 49.7% and 100%, respectively. When a histological diagnosis of LSIL+ is classified as positive, the sensitivity and specificity for HCII was 78.9% and 78.1%, respectively. The concordance ratio between the cytological and histological diagnosis was 80.4% (kappa=76.0) for LBC and 56.5% (kappa=55.1) for CC. LBC is more sensitive and less specific then CC, as a cytological cutoff level of ASCUS, but more sensitive and equally specific, as a cytological cutoff level LSIL or HSIL. LBC is more reliable with a high concordance ratio between the cytological and histological diagnosis.

• 한국미생물·생명공학회지
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• 제19권4호
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• pp.331-336
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• 1991

섬유성 물질을 발효기질로서 사용하기 위하여, 분리균 Pseudomonas sp. LBC-505의 cellulase를 이용하여 여러 종류의 천연 섬유성 물질에 대한 당화실험을 행하였다. Cellulase 복합체의 생산은 glucose에 의하여 저해되었고 CMC, avicel, 밀기울, 볏짚 등의 섬유성 물질에 의해 유도되었으며, 55(w/v) 밀기울 배지에서 최대 효소활성을 나타내었다. CMCase 와 xylanase의 최적 효소 반응온도는$50^{\circ}C$였으며, $\beta$-glucosidase는 $55^{\circ}C$ 였다. 또한, 이들 효소의 최적 반응 pH 는 모두 6.6이었다. 조효소 단독처리에 의한 천연섬유소의 당화율은 낮게 나타났으나, 5%(v/v) HCl로 실온에서 24시간 전처리한 후 효소반응을 행한 결과 가수분해율이 18.4%(w/w)로 볏집이 가장 양호하였으며, 구성당은 주로 glucose, xylose 및 cellobiose 였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7885-7889
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• 2014

Background: Activating mutations of epidermal growth factor receptor (EGFR) could predict response to tyrosine kinase inhibitor (TKI) treatment in patients with non-small cell lung cancer (NSCLC). However, the detection of EGFR mutation is frequently challenging in clinical practice for the lack of tumor tissue. The aim of this study was to investigate the feasibility of performing EGFR mutation testing on various types of liquid-based cytology (LBC) samples. Materials and Methods: A total of 434 liquid-based cytology samples were collected from March 2010 and November 2013. Among them, 101 with diagnosis of lung adenocarcinoma had paired surgically resected specimens. The ADx Amplification Refractory Mutation System (ADx-ARMS) was used to determine EGFR mutation status both in LBC and resected samples. Results: All liquid-based cytology samples were adequate for EGFR mutation analysis. The mutation rate was 50.5% in the 434 NSCLC patients with LBC samples and the incidence rates of EGFR mutation were consistent among different specimens. We also detected EGFR positives in 52.5% (53/101) patients with paired histologic specimens. The concordance rate of EGFR mutation between LBC samples and paired histologic specimens was 92.1%. Conclusions: Our results suggest that liquid-based cytology samples are highly reliable for EGFR mutation testing in patients with NSCLC.

• 대한치과기공학회지
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• 제46권2호
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• pp.28-35
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• 2024

Purpose: To evaluate the three-dimensional trueness of upper and lower denture bases produced using a digital light processing (DLP) printer and immersed in a constant-temperature water bath. Methods: An edentulous model was prepared and fitted with denture bases and occlusal rims manufactured using base plate wax. After scanning the model, denture bases, and occlusal rims, complete denture designs were created. Using the designs and a DLP printer, 10 upper and 10 lower complete dentures were manufactured. Each denture was scanned before (impression surface of upper denture base before constant temperature water bath [UBC] and impression surface of lower denture base before constant temperature water bath [LBC] groups) and after (impression surface of upper denture base after constant temperature water bath [UAC] and impression surface of lower denture base after constant temperature water bath [LAC] groups) immersion in the constant-temperature water bath. Scanned files were analyzed by comparing reference and scanned data, with statistical analysis conducted using the Kruskal-Wallis test (α=0.05). Results: Statistical analysis revealed no significant differences between the UBC and LBC groups, nor between the UAC and LAC groups (p>0.05). However, significant differences were observed between the UBC and UAC groups and between the LBC and LAC groups, i.e., before and after the constant-temperature water bath for both maxillary and mandibular denture bases (p<0.05). Conclusion: Denture bases not immersed in the constant-temperature water bath (UBC and LBC groups) exhibited error values within 100 ㎛, whereas those immersed in the water bath (UAC and LAC groups) showed error values exceeding 100 ㎛.