• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.4
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• pp.259-266
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• 2021

The present study was aimed to estimate the effect of ensiling period and bacterial inoculants on chemical compositions and fermentation characteristics on rye silage harvested at delayed stage. Rye (Secale cereale L.) was harvested after 20 days of heading stage (29.4% dry matter, DM). The harvested rye forage was applied with different inoculants following: applications of distilled water (CON), Lactobacillus brevis (LBB), Leuconostoc holzapfelii (LCH), or mixture of LBB and LCH at 1:1 ratio (MIX). Each forage was ensiled into 20 L mini bucket silo (5 kg) for 50 (E50D) and 100 (E100D) days in triplicates. The E50D silages had higher in vitro digestibilities of DM (IVDMD, p<0.001) and neutral detergent fiber (IVNDFD, p=0.013), and lactate (p=0.009), and acetate (p=0.011) than those of E100D, but lower pH, lactic acid bacteria (LAB), and yeast. By inoculant application, LCH had highest IVDMD and IVNDFD (p<0.05), while MIX had highest lactate and lowest pH (p<0.05). The CON and LCH in E50D had highest LAB and yeast (p<0.05), whereas LBB in E100D had lowest (p<0.05). Therefore, this study concluded that LCH application improved the nutrient digesbility (IVDMD and IVNDFD) of lignified rye silage, and longer ensiling period for 100 days enhanced the fermentation characteristics of silage compared to ensiling for 50 days.

• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.297-300
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• 1996

LBD-001, a recombinant human interferon $\gamma$ produced by genetically engineered yeast as a host system, was administered intraperitoneally to Sprague-Dawley male rats from premating to mating period at least for 60 days and to female rats from at least for 2 weeks before mating to early gestation period (from day 0 to 7 of gestation) at dose levels of $0.35\times10^6, 0.39\times10^6, and 1.38\times10^6$ I.U./kg/day. In the positive control group, ethynylestradiol ($EE_2$; 40 $\mu\textrm{g}$/kg/day) was subcutaneously administered only to female rats during the early gestation period. Effects of the test agents on reproductive performances of the male or female rats and embryonic development were as followings; (1) No significant changes by the treatment of LBD-001 were observed in general behaviors, body weight, food and water consumption, and necropsy of parent animals. However, significant decreases of body weight, food consumption, and water consumption were observed in ($EE_2$ -treated female rats. (2) Mating performances and fertility of parent animals were not significantly affected by the treatment of LBD-001. In ($EE_2$ -treated females, however, the fertility was completely inhibited. (3) No changes in resorption rate and external abnormality of F1 fetuses were observed by the treatment of LBD-001. The results show that LBD-001 at the dose of $1.38\times10^6$ I.U./kg/day or less does not affect general toxicity and reproductive function of parent animals and embryonic development of F1 fetuses.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.10 s.181
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• pp.2405-2412
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• 2000

A cast stainless steel may experience an embrittlement when it is exposed to approximately 30$0^{\circ}C$ for long period. In the present investigation, The three classes of the thermally aged CF8M specimie n are prepared using an artificially accelerated aging method. Namely, after the specimen are held for 300, 1800 and 3600hrs. at 43$0^{\circ}C$ respectively, the specimens are quenched in water to room temperature. Load versus load line displacement curves and J-R curves are obtained using the unloading compliance method. $J_{IC}$ values are obtained following ASTM E 813-87 and ASTM E 813-81 methods. In addition to these methods, JIC values are obtained using SZW(stretch zone width) method described in JSME S 001-1981. The results of the unloading compliance method are $J_Q$=485.7 kJ/m$^2$ for virgin material, $J_{IC}$ of the degraded materials associated with 300, 1800 and 3600hrs are obtained 369.25 kJ/m$^2$, 311.02 kJ/m$^2$, 276.7 kJ/m$^2$, respectively. The results of SZW method are similar to those of the unloading compliance method. Through the elastic-plastic fracture toughness test, it is found that the value of $J_{IC}$ is decreased with increasing of the aging time. The results obtained through the investigation can provide reference data for a leak before break(LBB) of reactor coolant system of nuclear power plants.