• Research in Plant Disease
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• v.28 no.2
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• pp.92-97
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• 2022

Apple stem grooving virus (ASGV) is a high-risk viral pathogen that infects many types of fruit trees, especially pear and apple, and causes serious economic losses across the globe. Thus, rapid and reliable detection assay is needed to identify ASGV infection and prevent its spread. A reliable reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed, optimize, and evaluated for the coding region of coat protein of ASGV in pear leaf. The developed RT-LAMP facilitated the simple screening of ASGV using visible fluorescence and electrophoresis. The optimized reaction conditions for the RT-LAMP were 63℃ for 50 min, and the results showed high specificity and 100-fold greater sensitivity than the reverse transcription polymerase chain reaction. In addition, the reliability of the RT-LAMP was validated using field-collected pear leaves. Furthermore, the potential application of paper-based RNA isolation, combined with RT-LAMP, was also evaluated for detecting ASGV from field-collected samples. These assays could be widely applied to ASGV detection in field conditions and to virus-free certification programs.

• Journal of Advanced Marine Engineering and Technology
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• v.34 no.4
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• pp.515-521
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• 2010

This paper dealt with the design and fabrication of an energy saving light emitting diode (LED) fishing lamp. Most fishes such as a squid, horse mackerel, mackerel, sardine and scabbard fish have characteristics for phototaxis and fishing lamps have promoted the fishery efficiency using their photo-reaction. In these days, metal halide lamp (MHL) as the fishing lamp, which consumes 1.5 kW and radiates harmful ultraviolet rays are mainly used. To develop the LED-fishing lamp, the penetration depth in sea water and the photo-reaction of a squid as light wavelength were studied. The experimental results showed the both characteristics were existed in blue color around 470 nm. Based on the results, we manufactured a 160 W and blue LED-fishing lamp which is consume about one-nine of 1.5 kW MHL. As energy saving effect, the use of LED-fishing lamp can reduce 128 kWh per an hour which is correspond to $CO_2$ of 86 kg for a 22ton-fishing boat equipped with 80-1.5 kW MHL. Now, the prototype LED fishing lampsare being evaluated on two fishing boats.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.297-303
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• 2013

This study was carried out to examine the effect of Red LED (660 nm) and fluorescent lamp for night break (NB) treatments of each 3 hours (22:30-01:30), 4 hours (22:00-02:00) and 5 hours (21:30-:02:30) per day for 53 days on flower bud initiation and growth in Chrysanthemum cv. 'Baekma' and cv. 'Jinba'. The days to flower budding after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (21.0 days) and 5 hr (20.5 days) NB, and it was shorter at Red LED 3 hr (14.2 days). The days to flowering after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (54.0 days), 5 hr (53.5 days) NB, and Red LED 5 hr (53.3 days), and it was shortest at Red LED 3 hr (50.2 days) NB treatment among all treatments. The days to flower budding after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (20.6 days) and was shorter at Red LED 3 hr (14.1 days) among all treatments. Similarly, the days to flowering after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (55.3 days) and was shortest at Red LED 3 hr (50.2 days) among all treatments. Therefore, inhibition of flower bud initiation was the most effective under fluorescent lamp 4 hr treatment. The length of cut flower of 'Baekma' was increased by fluorescent lamp 4 hr, 5 hr, and Red LED 5 hr, but of 'Jinba' was longer at LED 4 hr and 5 hr treatment. The weight of cut flower of 'Baekma' was heaviest at fluorescent lamp 5 hr treatment and was at Red LED 5hr treatment for 'Jinba' even though there was not statistically significant difference between 'Baekma' and 'Jinba'. Consequently, under fluorescent lamp 4 hr for night break was the most effective on flower bud initiation, flowering inhibition and cut-flower characteristics in 'Baekma' and 'Jinba'.

• Restorative Dentistry and Endodontics
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• v.28 no.3
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• pp.195-202
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• 2003

The purpose of this study was to evaluate the color changes of the composite resin resulting from xenon lamp exposure in different environments. Composite resin (Z 250 ; shade Al, A2, A3, A3.5, and A4) were applied in a cylindrical metal mold. Seventy five specimens according to environments of exposure were made as follows; Group I aluminum foiling of the specimens in the air at $37^{\circ}C$ for 1 day and 7 days. Group II : exposure of xenon lamp to the specimens in the air at $37^{\circ}C$ for 1 day and 7 days. Group III: exposure of xenon lamp to the specimens in distilled water at $37^{\circ}C$ for 1 day and 7 days. The color characteristics ($L^{*},a^{*},b^{*}$) of the specimens before and after exposure of xenon lamp were measured by spectrophotometer and the total color differences (${\Delta}E^{*}$) were computed. The results obtained were as follows : 1. In all groups except A1 shade of group III, the ${\Delta}E^{*}$ values presented below 2.0, and group III showed the highest ${\Delta}E^{*}$ values followed by group II and group I in a decreasing order(p<0.05). 2 In all shades and groups, the more the exposure time of xenon lamp and the lighter the shade were, the higher the tendency for discoloration (p<0.05). 3. The composite resins which was exposed to xenon lamp in the distilled water was more discolored than those in the air (p<0.05). 4. The major changes of composite resins which were exposed to xenon lamp in the air were an increase in yellowness through a positive shift of the $b^{*}$ value, and those in the distilled water were an increase in darkness and yellowness through a negative shift of the $L^{*}$ value and a positive shift of the $b^{*}$ value.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.3
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• pp.184-192
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• 1988

Anchovy, Engraulis Japonica were caught by scoop net with fishing lamp in the surrounding water of Cheju and Seogwipo, and their gathering depth, gathering effects, change of catch by the age of the moon and submarine illumination were investigated from May to August 1985. Fish finder (SR-385) and fishing lamp (1 Kw incandescent) were set up at one meter of starboard of scoop net and one meter ahead of the prow together with two meters above the water surface respectively. The submarine illumunation was measured at 2m interval to both vertical direction of 0~18m and horizontal direction of 0~12m form the standard point which is to be 0.1m depth right under the fishing lamp. The catch of anchovy by scoop net was almost $90\%$ of total amount during the early period and the late period in moon age while as low as $10\%$ only was cought during the middle period. The catching depth of anchovy shoals by scoop net with fishing lamp was approximately 2~5m and submarine illuminations were 20~42 Lux, 24~48 Lux in Cheju and Seogwipo respec lively. Submarine illumination which could be cought by scoop net with fishing lamp should be 7~12 times lighter than before gathering since the shoals swiming at 10~15m depth which is 1.7~7 Lux illumination made by 1 Kw. AC 100V incandescent lamp, a surface gathering lamp of 2m high above anchovy scoop net came up to 2~5m depth which is 20~42 Lux illumination. The catching depth of anchovy by scoop net was 2~3m and this could be increased to 4m even though the AC voltage was decreased from 100V to 80V at final fishing stage.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.493-496
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• 2016

Peach rosette mosaic virus (PRMV) is a plant virus that was first reported in 1933 by Peach. It can infect hosts including peach, grape, blueberry, dandelion, plum, cherry tree, and weeds. PRMV is non-reportable in Korea, but it is designated as a controlled virus requiring plant quarantine. In this study, for the rapid and specific detection of PRMV, we developed an assay using loop-mediated isothermal amplification (LAMP). Comparison between conventional polymerase chain reaction (PCR) methods (real time-PCR and nested PCR) and LAMP for the detection of PRMV revealed an equivalent level of sensitivity by all the tested methods. For the LAMP assay, outer primer sets were used to amplify a 264-bp PCR product, which was then digested using the restriction enzyme Pvu II (CAG/CTG), and the visualization of two digestion fragments (207 + 57 bp) indicated a positive reaction. The developed LAMP assay for PRMV is expected to enable the rapid monitoring of PRMV in plants.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.711-720
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• 2009

Characteristics of spectral irradiance based on the distance from the light source, which combined metal halide lamp and high-luminance light-emitting diode (LED) light, were studied to investigate a suitable operating method for fishing lamps of the next generation. A 380-780 nm wavelength radiation was superior when using 1 W electrical power in the order of metal halide lamp, blue LED, white LED, and combined LED lights. The wavelengths at which the irradiance was at a maximum were fixed to 581 nm for the light source, which was combined for each ratio. If the irradiance characteristics at 300-1100 nm wavelengths were set as 100%, the irradiance rates at 400-599 nm were 100%, 72.7%, 88.9%, and 69.5% for the blue, white, combined LED lights, and metal halide lamp, respectively. This indicated that the color rendering of the LED lights was dependent on the metal halide lamp light source. When the horizontal profiles (450-550 nm wavelength) of irradiances were compared to a different type of light source in the ratio white LED: combined LED lights: blue LED: metal halide lamp, the irradiated area of more than $0.01\;{\mu}mol/s/m^2/nm$ was in the ratio 1.0 : 1.3 : 1.7 : 37.3, respectively. Based on the radiation characteristics and irradiance according to the distance from the light source, LED lights have an estimated economic efficiency if used before and after operation of a metal halide lamp.

• Parasites, Hosts and Diseases
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• v.59 no.1
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• pp.15-22
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• 2021

Artemisinin resistance (ART) has been confirmed in Greater Mekong Sub-region countries. Currently, C580Y mutation on Pfkelch13 gene is known as the molecular marker for the detection of ART. Rapid and accurate detection of ART in field study is essential to guide malaria containment and elimination interventions. A simple method for collection of malaria-infected blood is to spot the blood on filter paper and is fast and easy for transportation and storage in the field study. This study aims to evaluate LAMP-SNP assay for C580Y mutation detection by introducing an extra mismatched nucleotide at the 3' end of the FIP primer. The LAMP-SNP assay was performed in a water bath held at a temperature of 56℃ for 45 min. LAMP-SNP products were interpreted by both gel-electrophoresis and HNB-visualized changes in color. The method was then tested with 120 P. falciparum DNA from dried blood spot samples. In comparing the LAMP-SNP assay results with those from DNA sequencing of the clinical samples, the 2 results fully agreed to detect C580Y. The sensitivity and specificity of the LAMP-SNP assay showed 100%. There were no cross-reactions with other Plasmodium species and other Pfkelch13 mutations. The LAMP-SNP assay performed in this study was rapid, reliable, and useful in detecting artemisinin resistance in the field study.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.49 no.4
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• pp.385-394
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• 2013

This study has conducted a comparative analysis on the fishing efficiency of LED fishing lamps by squid jigging vessels, the Yeongrak-ho (16 tons) and Somang-ho (9.77 tons), which operated during September and October 2010 and during October 2011, comparing with MH (Metal Halide) fishing lamp-equipped fishing vessels. This study has also examined vessel's fuel consumption level. The light powers of LED fishing lamps of the Yeongrak-ho and Somang-ho were 25.8kW and 32kW, respectively. Those of the MH fishing vessels, that is, the MH fishing lamp-equipped fishing vessels, were 105kW and 81kW, respectively. The average squid catch in number of an LED vessel, Yeongrak-ho, was 39.2% of the MH fishing lamp-equipped fishing vessels; however, that of the Somang-ho improved to 78.7% of the MH fishing lamp-equipped vessels. Average catch in number by Yeongrak-ho crew was 2.6 times more than catch in number by automatic jigging machines. Average catch in number by MH fishing vessel crew was 1.8 times more than that by automatic jigging machines. An LED vessel, Yeongrak-ho's fishing rate was 17.5%~152.2% of the MH fishing vessels, that is, 61.1% on average, in comparison of combined catch in number per automatic jigging machine and per crewmember. Somang-ho's fishing rate was 6.7%~127.6% of the MH fishing vessels, that is, 73.1% on average. The average fuel consumption level of the Somang-ho, throughout its departure from to arrival at the port, was 475.7l, and that during fishing hours was 109.6l, or 23.0% of the total fuel consumption level. Somang-ho's fuel consumption level per fishing hour was 9.7l on average.

• Journal of the Korea Convergence Society
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• v.8 no.3
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• pp.199-204
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• 2017

The Nowadays, LED head lamp has been used instead of halogen lamp at automotive head lamp. Inside this LED head lamp, there is a radiant heat panel which has the role to have the considerable influence upon LED life. As the radiant heat panel absorbs the heat from medium, the overheat of diode can prevented. in this study, the heat transfer and thermal stress are investigated according to the configuration of radiant heat panel. As the result of this study, the heat at model 1 decreases in comparison with model 2 and model 1 in design has the durability more than model 2. As the convergence technique is grafted on the design of radiant heat panel, the esthetic sense can be shown at the automotive head lamp.