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Ensiling of Sweet Potato Leaves (Ipomoea batatas (L.) Lam) and the Nutritive Value of Sweet Potato Leaf Silage for Growing Pigs

  • An, Le Van;Lindberg, Jan Erik
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.4
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    • pp.497-503
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    • 2004
  • The effect of adding carbohydrate-rich feedstuffs to sweet potato leaves (SPL) on silage quality was studied using a total of 180 laboratory silos. Silage quality was assessed by changes of pH, dry matter (DM), crude protein (CP) and ammonia nitrogen ($NH_{3}$-N). Pre-wilted SPL was mixed with cassava root meal (CRM), sweet potato root meal (SPM) or sugar cane molasses (Mo) at levels of 0, 30, 60 and 90 g $kg^{-1}$ (air-dry weight of additives to pre-wilted weight of SPL). Samples for assessing silage quality were collected after mixing the SPL with the additive and thereafter at 7, 14, 28 and 56 days of ensiling. There was a marked decrease in pH after 7 days and the pH remained low and stable until day 56. Addition of 60 and 90 g $kg^{-1}$ resulted in a lower pH (p<0.05) than the other treatments. The DM content of the silage increased (p<0.05) with increasing levels of additive, while there were no differences in DM with time of ensiling. The CP content of the silage decreased (p<0.05) with increasing levels of additive. The CP content did not change up to 28 days, but was lower (p<0.05) after 56 days in all treatments. The $NH_{3}$-N levels were increasing (p<0.05) with time of ensiling, and were lower (p<0.05) with additive levels of 60 g $kg^{-1}$ or higher. Also, the additive source affected the $NH_{3}$-N values, with the lowest values found for Mo. Castrated male pigs (Large White$\times$Mongcai) were used in 4$\times$4 Latin square design to study the total tract digestibility and nitrogen (N) utilisation of diets with inclusion of ensiled SPL. The diets were based on cassava root meal with inclusion of protein from either fish meal (C) or SPL ensiled with CRM (D1), SPL ensiled with SPM (D2) and SPL ensiled with Mo (D3). The digestibility of DM, organic matter (OM) and CP were higher (p<0.05), and the digestibility of crude fibre (CF) was lower (p<0.05), in diet C than in diets D1, D2 and D3. However, there were no differences (p>0.05) in digestibility of dietary components between diets D1, D2 and D3. Also, the excretion of N in faeces was higher (p<0.05) and the N retention was lower (p<0.05) in diets D1, D2 and D3 than in diet C. It can be concluded from the present experiments, that a good quality silage can be produced from pre-wilted SPL by addition of 60 g $kg^{-1}$ of either CRM, SPM or Mo. Diets with inclusion of 450 g ensiled SPL $kg^{-1}$ DM showed a high digestibility of dietary components and thus ensiled SPL should be considered as a potential feed resource for growing pigs.

Effect of Virus-free Plant and Subsoiling Reversion Soil for Reduction of Injury by Continuous Cropping of Sweet Potato (고구마 연작장해 경감을 위한 바이러스 무병묘 재배와 심토반전 효과)

  • Song, Hae-Ahn;Kim, Kab-Cheol;Lee, Seung-Yeob
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.254-261
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    • 2012
  • To reduce the injury by continuous cropping of sweet potato (Ipomoea batatas (L.) Lam.), the farmer's plant and virus-free plant were cultivated with the density of $70{\times}25cm$ (June 10, 2011) in continuous cropping soil (CCS) and subsoiling reversion soil (SRS). Fertilizer was applied at the rates of 55-63-156 $kg\;ha^{-1}$ ($N-P_2O_5-K_2O$) and 10 $ton\;ha^{-1}$ of cattle manure in CCS, and it was applied the 50% increased cattle manure compost and nitrogen in DRS. Symptoms of viral infection were revealed in the farmer's plant at 30 days after planting, but there were no symptoms in virus-free plant. The yield of virus-free plant was more increased 15% and 10.5% than that of farmer's plant in DRS and CCS, respectively. The yield of sweetpotato in SRS was more increased 8.8% and 3.2% in farmer's plant and virus-free plant compared to CCS, respectively. In DRS, the rate of marketable tuber of virus-free plant was increased by 80% compared to the farmer's plant (60.1%). The virus-free plant was produced the tuber with more brilliant peel color and well-formed shape compared to the farmer's plant. The increased yield of virus-free plant and in SRS soil condition showed a positive relationship (p=0.05) with the number of leaf per plant at 30 days and the number of branch per plant at 120 days after planting. The results showed that the early growth after planting was very important for the development of storage root. Therefore, the deep-subsoil reversion and cultivation of virus-free plant could be reduced the injury by continuous cropping of sweet potato, and increased farm income.

Difference of Growth and Root Characteristics of Sweetpotato by Cultivated Region (재배지역에 따른 고구마의 생육 및 괴근 특성 차이)

  • Han, Seon-Kyeong;Song, Yeon-Sang;Ahn, Seung-Hyun;Lee, Hyeong-Un;Lee, Joon-Seol;Chung, Mi-Nam;Park, Kwang-Geun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.3
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    • pp.262-270
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    • 2012
  • This research was performed to find out the root characteristics of sweetpotato (Ipomoea batatas LAM.) cultivars according to the cultivation regions. Total 10 sweetpotato culivars, 6 yellow, 2 purple and 2 orange, were used for experiment. Samples were cultivated in Muan, Iksan, Nonsan, Boryeong and Hamyang. Precipitation and average temperature during the growth period of sweetpotato cultivation were 882~1,682 mm and 16.7~$28.2^{\circ}C$, respectively. Accumulated temperature was 3,122~$3,282^{\circ}C$. Soil texture was found of sandy loam in Muan, Iksan and Boryeong, sandy clay loam in Nonsan, and loam in Hamyang. The yield of root, dry matter content, starch value and soluble solids contents were high in Muan. The length/width ratio was high in Hamyang. The color values of sweetpotatoes were high in Nonsan. The protein content of sweetpotato powder was high in the Iksan, crude fat content and ash content were high in the Hamyang. The results of this study, we could see that root characteristics of sweetpotato in the same cultivars appeared differently depending on the cultivated regions.

Enhanced drought and oxidative stress tolerance in transgenic sweetpotato expressing a codA gene (CodA 고발현 형질전환 고구마의 산화 및 건조 스트레스 내성 증가)

  • Park, Sung-Chul;Kim, Myoung Duck;Kim, Sun Ha;Kim, Yun-Hee;Jeong, Jae Cheol;Lee, Haeng-Soon;Kwak, Sang-Soo
    • Journal of Plant Biotechnology
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    • v.42 no.1
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    • pp.19-24
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    • 2015
  • Glycine betaine (GB) is one of the compatible solutes that accumulate in the chloroplasts of certain halotolerant plants under salt or cold stress. The codA gene for choline oxidase, the enzyme that converts choline into GB, has been cloned from a soil bacterium Arthrobacter globiformis. We generated transgenic sweetpotato plants [Ipomoea batatas (L.) Lam] expressing codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated SC plants under oxidative and drought stresses. SC plants showed enhanced tolerance to methyl viologen (MV)-mediated oxidative stress and drought stress due to induced expression of codA. At $5{\mu}M$ of MV treatment, all SC plants showed enhanced tolerance to MV-mediated oxidative stress through maintaining low ion leakage and increased GB levels compared to wild type plants. When plants were subjected to drought conditions, SC plants showed enhanced tolerance to drought stress through maintaining high relative water contents and increased codA expression compared to wild type plants. These results suggest that the SC plants generated in this study will be useful for enhanced biomass production on global marginal lands.

Effects of Seeding Method on the Winter Survival, Dry Matter Yield and Nutrient Value of Italian Ryegrass in Paddy Field (논에서 이탈리안 라이그라스의 파종방법이 월동, 건물수량 및 사료가치에 미치는 영향)

  • Kim, Meing-Jooung;Choi, Gi-Jun;Yook, Wan-Bang;Lim, Young-Chul;Yoon, Sei-Hyung;Kim, Jong-Geun;Park, Hyung-Soo;Seo, Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.4
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    • pp.269-274
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    • 2007
  • This experiment was conducted to determine the effect of seeding method on winter survival and dry matter yield of Italian ryegrass(Lolium multiflorum Lam. "Kospeed") in paddy field for two years. The experiment was arranged in randomized complete block design with three replications. The treatment consisted of four different seeding methods, R-LS-P(rotary-line sowing-packing), R-BS-P(rotary-broadcast seeding-packing), R-BS(rotary-broadcast seeding), R-BS-TR(rotary-broadcast seeding-trifling rotary), BS-TR(broadcast seeding-trifling rotary). The emergence ratio of broadcast was decreased by 12% and winter survival was decreased by 17% compared with line sowing. The R-BS-TR treatment which was practical seeding method of rye was decreased seeding stand ratio by 65.2% and winter survival by 6.7% compared with others seeding methods. Dry matter yield of line sowing(8,151 kg/ha) was higher than that of broadcast(6,281 kg/ha) and R-BS-P treatment was the highest DM yield as 7,166 kg/ha. There was not found significant difference among seeding method in forage quality. The results of this experiment indicated that R-BS-P(rotary-broadcast seeding-packing) was recommendable the best seeding method after harvested whole crop rice in paddy field.

Leaf Protein Affect the Concentration on Serum Cholesterol and Free Amino Acids in Rats (녹엽단백질 급여가 흰쥐 혈청 콜레스테롤 및 유리 아미노산 농도에 미치는 영향)

  • 조영수;김성규;김도훈;정순재;차재영
    • Journal of Life Science
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    • v.9 no.1
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    • pp.35-43
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    • 1999
  • Leaf protein (LP) was prepared by centrifugation the green juice from Italian ryegrass (Lolium multiflorum Lam.) at acid precipitation. LP was compared with casein and soybean protein isolate for their concentration of serum lipids and free amino acids in Wistar male rats for 2 weeks. Each protein source was incoporated into cholesterol free, low-fat diet to provide a protein level of 20%. A comparison of serum amino acids in leaf protein-fed vs. casein-fed rats showed that, glycine was the only amino acid having a higher concentration whereas concentration of other amino acids were lower in the leaf protein-fed rats compared with the casein-fed rats. Further alanine was significantly lower in the leaf protein-fed rats compared with the casein-fed rats, and the protein-induced differences in glycine and alanine concentration of unfed rats were reproducible. Concentration of several amino acids, rarticularly valine, leucine and tyrosine, also changed when serum cholesterol concentrations varied, but these effects could not be explained by our experiments. The results suggest that a change in serum concentration of glycine and alanine of unfed rats may be related to the change in serum cholesterol concentration. These data also suggest appear to function in a similar way to soybean protein in their hypocholesterolemic action.

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Growth Characters and Productivity of Italian Ryegrass (Lolium multiflorum L.) New Variety 'Hwasan 104' (이탈리안 라이그라스 신품종 '화산 104호'의 생육특성과 수량성)

  • Choi Gi Jun;Rim Yong Woo;Sung Byung Ryul;Lim Young Chul;Kim Meing Jooung;Kim Ki-Yong;Park Geun Je;Park Nam Keon;Hong Youn Ki;Kim Sang Rok
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.275-280
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    • 2005
  • This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 2002. Hwasan 104 as a tetraploid variety was dark green in leaf color and had semi-prostrate and medium growth habit in late autumn and early spring, respectively. Hwasan 104 was 19th May in heading date as a late-heading variety. Especially, Hwasan 104 was broader in flag leaf and longer 7 cm in plant length, and thicker in stem width than those of control variety, Hwasan 101. Cold tolerance of Hwasan 104 was better than that of Hwasan 101 registered as a cold-tolerant variety. Dry matter (DM) yield of Hwasan 104 was $5\%$ more than DM 9,348 kg/ha of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient of Hwasan 104 were 74.3 and $63.1\%$ which are 3.3 and $1.5\%$ lower than those of Hwasan 101, respectively. Acid detergent fiber and neutral detergent fiber of Hwasan 104 were 32.6 and $55.8\%$ which are 1.9 and $2.4\%$ higher than those of Hwasan 101, respectively.

Growth Characteristics and Nutritional Composition of Italian Ryegrass (Lolium multiflorum Lam.) Cultivars Grown in a Paddy Field (논토양에 이탈리안 라이그라스 재배 시 품종별 생육특성 및 영양성분 비교 연구)

  • Lee, Sang Moo;Kim, Eun Joong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.2
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    • pp.183-188
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    • 2017
  • This study investigated the comparative growth characteristics, yield, chemical compositions, and nutritive yield of Italian ryegrass cultivars harvested in a paddy field. The experimental design was a randomized block design with three replicates. The treatments consisted of seven Italian ryegrass cultivars (Kogreen, Kowinmaster, Hwasan 101, Kowinnearly, Kospeed, Sahalie select, and Strike). The planting date was October 21, 2009 and the sample harvest was occurred on May 21, 2010. Dry matter yield was higher in Kowinnerary and Kogreen than in the other varieties (p<0.05). Crude protein and ether extract contents were highest in Hwasan 101 and lowest in Kospeed. The content of total digestible nutrients did not differ among the Italian ryegrass varieties. The amino acid contents were in the order Hwasan 101 > Strike > Kowinnearly > Kogreen > Sahalie select > Kospeed > Kowinnearly (p<0.05). Fructose content was highest in Kospeed, while glucose and sucrose contents were highest in Hwasan 101. The results of this study indicate that Kowinnearly, in terms of dry matter yield, and Hwasan 101, in terms of feed value (chemical composition and free sugar content), may be recommended for forage production in a paddy field.

Research on Actual Usage and Satisfaction of ROKAF Fighter Pilot's Flight Duty Uniform (공군 전투조종사 비행복 착용특성 및 만족도 조사)

  • Lee, Ah Lam;Nam, Yun Ja;Hong, Yu Hwa;Im, Sojung;Lim, Chae Keun
    • Journal of the Korean Society of Clothing and Textiles
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    • v.40 no.4
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    • pp.669-684
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    • 2016
  • This study investigates Korean fighter pilot's usage and satisfaction of a flight duty uniform (FDU). The survey was conducted from October 2014 to March 2015 using Focus Group Interview (FGI) and questionnaires. FGI collected qualitative data about duty and requirements; subsequently, surveys were performed to collect quantitative data about wearing conditions and satisfaction with FDU. The results of the FGI and the questionnaire were as follows. Type of pilot duty was divided into two parts, flight duty and ground duty. It is important to consider duties as well as factors related to survival when developing FDU. According to anthropometric data and wearing size, the basic size for apparel grading should be changed from actual size, 'M95XL' to 'M100L'. It is also necessary to improve the whole sizing system. Further studies about body form changes in pilot's movement are needed to improve mobility because the respondents perceived some restrictions at several body parts in movement with the coverall uniform. Summer FDU had a low satisfaction level in vent hole function and appearance. Furthermore, protection problems in the vent hole were also an issue. Making a seasonal classification of FDU fabric will be more effective than a vent hole to increase a pilot's thermal satisfaction. Respondents had a passive stance towards FDU reform (including pocket change); therefore, a new FDU design strategy should concentrate on improving current FDU functions like mobility (or comfort) rather than dramatic changes. Pilots complained about the quality stability of FDU; therefore, quality control by military administration as well as concrete and clear design instructions by the developer should be attained together. The results obtained in this study are expected to be used as an important basis for the further development of FDU.

Extract of Moringa Root Inhibits PMA-induced Invasion of Breast Cancer Cells (유방암 세포주에서 PMA로 유도된 암세포 침투에 대한 모링가 뿌리 추출물의 억제효과)

  • Cho, Hyun-Ji;Chang, Young-Chae
    • Journal of Life Science
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    • v.24 no.1
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    • pp.8-13
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    • 2014
  • The moringa (Moringa oleifera Lam.) plant is used as food and as an anti-allergic agent. In this study, we studied the inhibitory effect of moringa root extract on the expression of PMA-induced matrix metalloproteinase-9 (MMP-9), which is the main factor implicated in the invasion and metastasis of cancer cells in MCF-7 cells. At first, various moringa extracts were examined in the MCF-7 cells. Both moringa root extract and leaf extracts inhibited PMA-induced MMP-9 activity, but the root extract suppressed PMA-induced MMP-9 activity to a greater extent than the leaf extract. The moringa root extract also inhibited PMA-induced MMP-9 protein expression and cell invasion. According to RT-PCR, the treatment of the MCF-7 cells with moringa root extract decreased levels of PMA-induced MMP-9 mRNA expression, but not the expression of TIMP-1 and -2, indicating that moringa root extract prevents the transcription of MMP-9 in response to PMA. In addition, moringa root extract specifically suppressed the phosphorylation of ERK/JNK, but not p38. We suggest that moringa root extract abolishes MMP-9 activity/expression through ERK/JNK. In conclusion, moringa root extract suppressed PMA-induced MMP-9 activity/expression by inhibiting the phosphorylation of ERK/JNK in MCF-7 cells. These results indicate that moringa root extract may be a potential antimetastatic and anti-invasive agent. Future clinical research is needed on the anticancer properties of moringa root extract.