• Title/Summary/Keyword: L20 Method

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Variation of Indoor Air Temperature by using Hot Water Piping in Greenhouse (온수배관에 의한 온실 내부의 온도변화)

  • Yoon, Yong-Cheol;Shin, Yik-Soo;Bae, Seoung-Beom;Kim, Hyeon-Tae;Choi, Jin-Sik;Suh, Won-Myung
    • Journal of agriculture & life science
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    • v.46 no.2
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    • pp.179-190
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    • 2012
  • This study was performed to obtain a heat saving effect and enhance the efficiency of a greenhouse by using a hot water piping in order to minimize the operating costs of a greenhouse as oil prices continue to rise. This method also reduces the likelihood of accidents caused by snowdrifts in regions with heavy snowfall. In general, the experimental plot was $2.0{\sim}6.0^{\circ}C$ higher than the control plot. When the skylight felt was opened, the minimum temperature was in the range of $3.0{\sim}12.0^{\circ}C$. Therefore, we judged that damage caused by snowdrifts may be prevented partly by active heating. The temperature difference inside of the greenhouse by height was insignificant. The maximum heating load of the greenhouse according to crop was respectively about $37,000kcal{\cdot}h^{-1}$ and $41,700kcal{\cdot}h^{-1}$. During the experiment, the heat value of each designed temperature in the range of the minimum ambient temperature $-11.9{\sim}4.0^{\circ}C$ was about 95,000~322,000 kcal and it was in the range of $6,050{\sim}20,900kcal{\cdot}h^{-1}$. If it is compared with the maximum heating load, it can be shown that about 15~56% of the heating energy can be supplied. The total heat value and the amount of power consumption were 2,629,025 kcal and 677.3 kWh respectively during the experiment. If it is heated with diesel, a fossil fuel, the consumption during the experiment was 291 L and the cost was 331,700won. Total cost of using electric power was about 24,400 won and it is shown that it is about 7.5% of the cost of diesel consumption. Also, if the total amount of power consumption is converted into energy, it is approximately 582,200 kcal and the energy was just about 22% of the total heat value.

A Loop-mediated Isothermal Amplification Method for White-backed Planthopper-specific Detection (고리매개등온증폭법(LAMP)을 이용한 흰등멸구 특이 판별법)

  • Seo, Bo Yoon;Park, Chang Gyu;Jung, Jin Kyo;Cho, Jumrae;Lee, Gwan-Seok;Kim, Kwang-Ho
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.393-399
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    • 2018
  • A loop-mediated isothermal amplification (LAMP) primer set (WBPH-65) was designed for the species-specific detection of white-backed planthopper (WBPH) Sogatella furcifera based on the full-length sequence of the internal transcribed spacer 2 (ITS2) (KC417469.1). The WBPH-65 primer set consists of six primers (total 165 bp), F3 (18 bp), B3 (18 bp), FIP (43 bp), BIP (40 bp), LF (21 bp), and LB (25 bp). After the LAMP reaction of three rice planthoppers, S. furcifera, Nilaparvata lugens, and Laodelphax striatellus, with the WBPH-65 primer set for 60 min at $65^{\circ}C$, the LAMP products were observed in the genomic DNA of S. furcifera only. According to the DNA amount of S. furcifera and incubation duration at $65^{\circ}C$, the difference of fluorescence relative to the negative control (0 ng) was clearly observed in a 40-min incubation with 10 and 100 ng or in case of 60-min incubation with 0.01, 0.1, 1, 10, and 100 ng. There was little difference in fluorescence between the negative control and all the other DNAs tested in 20- and 30-min incubations. On the other hand, the WBPH-65 primer set without LF and LB primers showed little amplification in the genomic DNAs of the three rice planthoppers, S. furcifera, N. lugens, and L. striatellus in a 60-min incubation. These results suggest that all six primers (F3, B3, FIP, BIP, LF, and BF) are necessary for the WBPH-65 primer set to detect S. furcifera within a 60-min incubation, and is able to discriminate S. furcifera from at least N. lugens and L. striatellus.

Effectiveness of Drain Insertion and Irrigation in the Treatment of Septic Arthritis of the Knee under Local Anesthesia (국소 마취하 배액관 삽입 및 세척을 통한 화농성 슬관절염의 치료의 효용성)

  • Yi, Jin Woong;Oh, Byung Hak;Heo, Youn Moo;Jang, Min Gu;Min, Young Ki;Seo, Kyung Deok
    • Journal of the Korean Orthopaedic Association
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    • v.56 no.4
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    • pp.310-316
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    • 2021
  • Purpose: Septic arthritis of the knee is an orthopedic emergency that requires early diagnosis and surgical treatment. This study examined the effectiveness of drain insertion and irrigation in the treatment of septic arthritis of the knee under local anesthesia. Materials and Methods: A retrospective study was conducted on nine cases (eight patients) diagnosed with septic arthritis of the knee from September 2017 to February 2020 and treated with drain insertion and irrigation under local anesthesia. After penetrating through the superolateral portal to the superomedial portal and inserting the drain, daily irrigation of approximately 3 L of normal saline was done. The following were investigated: age, sex, underlying disease, cause, degree of osteoarthritis, time from diagnosis to surgery, duration of hospitalization, duration of normalization of C-reactive protein, and smear and culture. Results: The initial white blood cell count of joint fluid was 71,472±51,667/mm3 (32,400-203,904/mm3), and polymorphic leukocytes were 91.1%±2.6% (86%-95%). The average time from diagnosis to surgery was 8.3±1.3 hours (6-10 hours), and the irrigation period was 8.2±3.2 days (4-15 days). The average length of hospitalization was 20.8±8.7 days (9-37 days). There was no reoperation or recurrence. Smear and culture tests were not identified. Conclusion: In the treatment of septic arthritis of the knee, the insertion of a drain tube and irrigation under local anesthesia is a relatively fast and simple method to reduce pain by repetitive draining of purulent joint fluid and can be used as an alternative treatment for patients with a risk of general or spinal anesthesia.

Evaluation of the Effect of Different Application Ratios of Lime-treated Fertilizer Mixed with Food Waste on Chinese Cabbage (Brassica rapa L.) Yield and Soil Chemical Properties (음식물류폐기물 혼합 석회처리비료 사용량에 따른 배추(Brassica rapa L.) 수량 및 토양 화학성 평가)

  • Young-Jae Jeong;Sang-Geum Lee;Seong-Heon Kim;Sang-Ho Jeon;Youn-Hae Lee;Soon-Ik Kwon;Jae-Hong Shim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.2
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    • pp.81-89
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    • 2023
  • Lime-treated fertilizer (LTF) is manufactured using the lime stabilization method with food waste. LTF is effective in neutralizing acidic soil, improving nutrient and organic matter content in soil, and increasing crop productivity. However, excessive use of LTF in agricultural land can have undesirable effects, such as reduced crop growth and nutrient accumulation in soil. This study was evaluated the effect of different application ratios of LTF on the crop yield index (%), nutrient (N, P2O5, K2O) uptake index (%), and soil chemical properties. The following treatments were applied: untreated (UT), NPK (NPK), NPK+calcium hydroxide (CH), and NPK+1-, 2-, 4-, and 8-times of LTF (LTF1, 2, 4, and 8). The yield index for LTF1 was the highest among different LTF treatments. Moreover the yield index for spring and winter cabbage in LTF1 treatment was 10% and 21% higher, respectively, than that in NPK treatment. The yield and nutrient indices were decreased with the increase in LTF application ratio. The soil pH and EC tended to increase with the increase in LTF ratio, and were the highest at 8.2 and 2.1, respectively, after cultivation for LTF8 (P<0.05). With the increase in soil pH, the soil inorganic nitrogen (NH4-N, NH3-N) and available phosphate (Av. P2O5) levels were decreased (P<0.05). Our results suggest that LTF1 (643 kg 10a-1) is an appropriate ratio for improving soil chemical properties and increasing crop yield.

Effect of Drying Type and Addition Level of Sweet Potato 'Tongchaeru' Byproducts on Broiler Productivity, Meat Quality, Blood Parameters, and Immune Response (고구마 '통채루' 부산물의 건조 방법과 첨가 수준에 따른 육계 생산성, 계육 품질, 혈액 성상, 면역 지표에 미치는 영향)

  • Woo-Do Lee;Hyunsoo Kim;Jiseon Son;Eui-Chul Hong;Hee-Jin Kim;Yeon-Seo Yun;Hye Kyung Shin;Hwan-Ku Kang
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.325-336
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    • 2023
  • This study used leaves and stems of 'Tongchaeru', one of the sweet potato varieties, to investigate broiler productivity, meat quality, blood properties, growth hormones, and immune factor levels according to drying method and amount added to feed. For this experiment, a total of 720 1-day-old male Ross 308 broilers were used. Treatments were assigned with 3 replicates per treatment and 20 birds were assigned to each replicate. The treatment group was designed into 12 treatments according to the type of natural product (leaves (L), stems (S)), drying type (natural (N), hot air (H), freeze (F)) and amount added (0.1%, 0.3%). The test was conducted for a total of 5 weeks. In this study, there was no significant difference in productivity depending on the type and amount of additives added (P>0.05). The FS 0.3% group showed high pH and WHC levels, and the shear force was lowest at HL 0.1% group (P<0.05). Blood cell and serum biochemical components were similar in all treatments, and growth hormone IGF-1 was highest in FS 0.1% group (P<0.05). There was no significant difference in IFN-γ, but the highest level of IL-6 was seen in the HS 0.1% group (P<0.05). In conclusion, the meat quality and the level of growth hormone and immune factors in the body were different depending on the type and amount of dried leaves and stems of sweet potato 'Tongchaeru', further study is needed to compare the selected additives and amounts added with those without additions.

A Clinical Evaluation of Splanchnic Nerve Block (내장신경차단에 관한 임상적 연구)

  • Kim, Soo-Yeoun;Oh, Hung-Kun;Yoon, Duek-Mi;Shin, Yang-Sik;Lee, Youn-Woo;Kim, Jong-Rae
    • The Korean Journal of Pain
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    • v.1 no.1
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    • pp.34-46
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    • 1988
  • Intractable pain from advanced carcinoma of the upper abdomen is difficult to manage. One method used to control pain associated with these malignancies is to block off the splanchnic nerve. In 1919 Kappis described a technique by which the splanchnic nerve of the upper abdomen could be anesthetized, using a percutaneous injection. This method has been used for the relief of upper abdominal pain due to hematoma and cancer of the pancreas, stomach, gall bladder, bile duct, and colon. During the Period from November 1968 to January 1986, this method was used in 208 cases of malignancy at Severance Hospital and clinically evaluated. Patients were retroactively grouped according to the stage of development of technique used. Twelve patients who received the treatment in the period from November 1968 to March 1977 were designate4i as group 1, 26 patients from April 1977 to April 1979 as group 2, and 170 from May 1979 to January 1986 as group 3. The results are as follows: 1) The number of patients receiving splanchnic nerve block has been increasing since 1977. 2) A total of 208 patients, including 133 males and 75 females, ranging in age from 18 to 84 and averaging 51. 3) The causes of pain were stomach cancer 90, pancreatic cancer 69, and miscellaneous cancer 49 cases respectively. 4) There were 57.7% who had surgery. and 3.7% of whom had chemotherapy before the splanchnic nerve block was done. 5) These blocks were carried out with the patient in the prone position as described by Dr. Moore. For group 2 and 3, C-arm image intensifier was used. In group 1, a 22 gauze loom long needle was inserted at the lower border of the 12th rib on each aide about 7\;cm from the midline. The average distance from the midline was $6.60{\pm}0.61\;cm$ on the left side and $6.60{\pm}0.83\;cm$ on the right side in group 2, and $5.46{\pm}0.76\;cm$ on the left side and $5.49{\pm}0.69\;cm$ on the right side in group 3. The average depth to which the needle was inserted was $8.60{\pm}0.52\;cm$ on the left side and $8.74{\pm}0.60\;cm$ on the right side in group 2, and $8.96{\pm}0.63\;cm$ on the left side and $9.18{\pm}0.57\;cm$ on the right side in group 3. 6) The points of the inserted needles were positioned in the upper quarter anteriorly, 51.8% on the left side and 54.4% n the right side of the L1 vertebra by lateral roentgenogram in group 3. The inserted needle points were located in the upper and anterolateral part, of the L1 vertebra 68.5% on the left side and 60.6won the right side, on the anteroposterior rentgenogram in group 3. The needle tip was not advanced beyond the anterior margin of the vertebral body. 7) In some case of group 3, contrast media was injected before the block was done. It shows, the spread upward along the anterior mal gin of the vertebral body. 8) The concentration and the average amount of drug used in each group was as follows: In group 1, $39.17{\pm}6.69\;ml$ of 0.5% -l% lidocaine or 0.25% bupivacaine were injected for the test block and one to three days after the test block $40.00{\pm}4.26\;ml$ of 50% alcohol was injected for the semipermanent block. In group 2, $13.75{\pm}4.88\;ml$ of 1% lidocaine were used as the test block and followed by $46.17{\pm}4.37\;ml$ of 50% alcohol was injected as the semipermanent block. In group 3, $15.63{\pm}1.19\;ml$ of 1% lidocaine for test block followed by $15.62{\pm}1.20\;ml$ of pure alcohol and $16.05{\pm}2.58\;ml$ of 50% alcohol for semipermanent block were injected. 9) The result of the test block was satisfactory in all cases. However the semipermanent block was 83.3 percent of the patients in group 1 who received relief from pain for at least 2 weeks after the block, 73.1% in group 2, and 91.8% in group 3. In these unsuccessful cases, 2 cases in group 1 were controlled by narcotics but 7 cases in group 2 and 14 cases in group 3 received the same splanchnic nerve block 1 or 2 times again within 2 weeks. But, in some cases it was 3 to i months before the 2nd block and in 1 cases even 7 years. 10) The most common complications of splanchnic nerve block were hypotensino(25.5%) occasional flushing of the face, nausea, vomiting, and chest discomfort. 11) For the patients in group 3, the supplemental block most commonly used was a continuous epidural block; it was used as a diagnostic block and to afford relief from pain before the splanchnic nerve block was done. 12) The interval between the receiving of the alcohol block and discharge was from 5 to 8 days in 61 cases(31.1%) and from 1 to 2 days in 48 cases(24.5%). From the above results, it can be concluded that the splanchnic nerve block done in the prone position with pure and 50% alcohol immediately after an effective test block with 1% lidocaine under C-arm fluoroscopic control is satisfactory and reliable. How to minimize the repeat block is still a problem to be solved.

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Changes of the Prefrontal EEG(Electroencephalogram) Activities according to the Repetition of Audio-Visual Learning (시청각 학습의 반복 수행에 따른 전두부의 뇌파 활성도 변화)

  • Kim, Yong-Jin;Chang, Nam-Kee
    • Journal of The Korean Association For Science Education
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    • v.21 no.3
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    • pp.516-528
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    • 2001
  • In the educational study, the measure of EEG(brain waves) can be useful method to study the functioning state of brain during learning behaviour. This study investigated the changes of neuronal response according to four times repetition of audio-visual learning. EEG data at the prefrontal$(Fp_{1},Fp_{2})$ were obtained from twenty subjects at the 8th grade, and analysed quantitatively using FFT(fast Fourier transform) program. The results were as follows: 1) In the first audio-visual learning, the activities of $\beta_{2}(20-30Hz)$ and $\beta_{1}(14-19Hz)$ waves increased highly, but the activities of $\theta(4-7Hz)$ and $\alpha$ (8-13Hz) waves decreased compared with the base lines. 2). According to the repetitive audio-visual learning, the activities of $\beta_{2}$ and $\beta_{1}$ waves decreased gradually after the 1st repetitive learning. And, the activity of $\beta_{2}$ wave had the higher change than that of $\beta_{1}$ wave. 3). The activity of $\alpha$ wave decreased smoothly according to the repetitive audio-visual learning, and the activity of $\theta$ wave decreased radically after twice repetitive learning. 4). $\beta$ and $\theta$ waves together showed high activities in the 2nd audio-visual learning(once repetition), and the learning achievement increased highly after the 2nd learning. 5). The right prefrontal$(Fp_{2})$ showed higher activation than the left$(Fp_{1})$ in the first audio-visual learning. However, there were not significant differences between the right and the left prefrontal EEG activities in the repetitive audio-visual learning. Based on these findings, we can conclude that the habituation of neuronal response shows up in the repetitive audio-visual learning and brain hemisphericity can be changed by learning experiences. In addition, it is suggested once repetition of audio-visual learning be effective on the improvement of the learning achievement and on the activation of the brain function.

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Increase in Anti-Oxidant Components and Reduction of Off-Flavors on Radish Leaf Extracts by Extrusion Process (압출성형 무청 분말 추출물의 항산화 물질 함량 증가 및 이취 감소)

  • Sung, Nak-Yun;Park, Woo-Young;Kim, Yi-Eun;Cho, Eun-Ji;Song, Hayeon;Jun, Hyeong-Kwang;Park, Jae-Nam;Kim, Mi-Hwan;Ryu, Gi-Hyung;Byun, Eui-Hong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.12
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    • pp.1769-1775
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    • 2016
  • Aerial parts (leaves and stems) of radish are usually discarded due to the distinct undesirable flavors associated with inappropriate preparations, despite their many health benefits. In this study, we examined the role of extrusion process in the removal of off-flavors and elevation of antioxidant activity in radish (Raphanus sativus L.) leaves and stems. To optimize the extrusion conditions, we changed the barrel temperature (110, 120, and $130^{\circ}C$), screw speed (150, 200, 250, and 300 rpm), and moisture content (20, 25, and 30%). The polyphenol and flavonoid contents significantly increased in extruded radish leaves and stems (ER) under optimum extrusion conditions ($130^{\circ}C$, 250 rpm, and 20%). Under extrusion conditions, we compared off-flavors (as amount of sulfur-containing compound) levels between ER and non-extruded radish leaves and stems (NER) by an electronic nose. A total of six peaks (sulfur-containing compound) were similarly detected in both ER and NER, whereas the ER showed reduced off-flavors. Levels of glucosinolate (${\mu}g/g$), which can be hydrolyzed into off-flavors during mastication or processing, were significantly decreased in the ER. From these results, extrusion processing can be an effective method to increase anti-oxidant activity and removal of off-flavors in radish leaves and stems.

Immobilization of Xylose Isomerase and Trial Production of High Fructose Corn Syrup (Xylose 이성화 효소의 고정화 및 이성화당의 생산)

  • Chun, Moon-Jin;Lim, Bun-Sam
    • Applied Biological Chemistry
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    • v.26 no.4
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    • pp.222-230
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    • 1983
  • This study was designed to develop a process for the immobilization of xylose isomerase(D-xylose ketol isomerase, EC 5.3.1.5) from Streptomyces griseolus previously isolated by the authors and its application on a pilot plant scale for the production of high fructose corn syrup. The biomass which has endo-excreted xylose isomerase was homogenized under a pressure of $500kg/cm^2$ and 90.8% of the enzyme recovery of the native activity was obtained as compared to 54.7% recovery by the lysozyme treatment. Ionic bonding method was adopted for the enzyme immobilization due to its many reported merits. It was found that the porous resins such as Diaion HP 20, Duolite A-7, Amberlite IRA 93 and 94 were effective in immobilizing the enzyme. In addition, it was disclosed that the regeneration form of $BO_4--$ is effective for Amberlite IRA 93 and $HCO_3-$ for Diaion HP 20. Optimal immobilization condition for Amberlite IRA 93 was pH 8.0 and $55^{\circ}C$ yielding 80.6% of immobilization. Activity decay test showed half life of the immobilized enzyme with Amberlite IRA 93 was more than 24 days at $65^{\circ}C$. The carrier was evaluated to be resuable and its result showed the relative immobilization yields were 98.2, 93.3, 90.7 and 87.5%, respectively at second, third, forth and fifth rebinding test of the enzyme on Amberlite IRA 93. Optimal temperature of the immobilized enzyme was slightly lowered and the range widened to $60\sim70^{\circ}C$, while optimal pH moved toward $8.0\sim8.3$ in its isomerization reaction. The trial production result of high fructose corn syrup in pilot scale immobilization showed that one liter of immobilized xylose isomerase (350 IXIU/ml-R) is capable producing about 293l high fructose corn syrup(75% dry substance) in 30 days.

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Is Premedication Necessary for Outpatient Fiberoptic Bronchoscopy (외래환자의 기관지내시경검사시 전처치의 필요성)

  • Won, Jun-Hee;Park, Jae-Yong;Cha, Seung-Ick;Kang, Tae-Kyong;Park, Ki-Su;Kim, Yeon-Jee;Kim, Chang-Ho;Jung, Tee-Hoon
    • Tuberculosis and Respiratory Diseases
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    • v.46 no.2
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    • pp.251-259
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    • 1999
  • Background: Even though the necessity for premedication has been questioned, some combinations of antisialogogues, sedatives and analgesics are usually employed by most bronchoscopists. The goal of this study was to determine whether fiberoptic bronchoscopy(FOB) could be performed safely and effectively without premedication while using a standardized topical anesthetic. Method: Eighty outpatients were randomized in a double-blind manner into group I(n=20) with 1 ml normal saline, group II(n=20) with 0.5mg of atropine, group III(n=20) with 0.5mg of atropine plus 5mg of midazolam, and group IV(n=20) with 0.5mg of atropine plus 50mg of meperidine, given intramuscularly 30 minutes before FOB as premedication Topical anesthetics administered were same in each group. Each patient was given 5ml(200mg) of 4% nebulized lidocaine and additional intratracheobronchial 2% lidocaine. Oxygen saturation, pulse rate and electrocardiogram were monitored and recorded before, during, just after and 2 minutes after FOB. Immediately after FOB, the bronchoscopists answered four questions such as ease of procedure, extent of coughing, extent of secretion, and overall impression. Before leaving bronchoscopy suite, patients completed similar questions on discomfort during procedure, and willingness to return for a repeat procedure. Results: Age, gender, baseline pulmonary function, dose of 2% lidocaine used for topical anesthesia, and duration of FOB were not significantly different. There was no statistical difference among group I to IV with regard to extent of coughing answered by bronchoscopist. But extent of secretion was significantly different between group I without atropine and group II-IV with atropine. And there was also significant difference in ease of procedure and overall impression among groups. There was no statistical difference in patient's willingness and level of discomfort among the groups. Thirteen patients(16%) showed hypoxemia(arterial oxygen saturation: <90%), and 3 patients(4%) showed significant tachyarrhythmia(heart rate: > l60/min), but the rate of complication was not statistically different among the groups. Conclusion: These results suggest that the value of premedication is questionable for outpatient FOB, although it may be necessary in excessively anxious patient.

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