• Title/Summary/Keyword: L132

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Effects of Lactic Acid Bacteria Inoculant on Fermentation Quality and in vitro Rumen Fermentation of Total Mixed Ration

  • Choi, Yeon Jae;Lee, Sang Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.3
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    • pp.132-140
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    • 2019
  • Fermented total mixed ration (TMR) is a novel feed for ruminants in South Korea. The purpose of this study was to evaluate the effects of lactic acid bacteria (LAB) on the quality of TMR and in vitro ruminal fermentation. Strains of three LAB spp. (Lactobacillus plantarum, L. brevis, L. mucosae) were used in fermentation of TMR. Inoculations with the three LAB spp. lowered pH and increased concentrations of lactic acid, acetic acid, and total organic acid compared to non-LAB inoculated control (only addition of an equivalent amount of water) (p<0.05). Bacterial composition indicated that aerobic bacteria and LAB were higher. However, E. coli were lower in the fermented TMR than those in the control treatment (p<0.05). Among the treatments, L. brevis treatment had the highest concentration of total organic acid without fungus detection. Gas production, pH, and ammonia-nitrogen during ruminal in vitro incubation did not differ throughout incubation. However, ruminal total VFA concentration was higher (p<0.05) in the LAB spp. treatments than the control treatment at 48 hours. Overall, the use of L. brevis as an inoculant for fermentation of high moisture. TMR could inhibit fungi growth and promote lactic fermentation, and enhance digestion in the rumen.

A Study of the Presence of Carbonic Acid and Other Potentially Hazardous Substances in Cheongsong Mineral Water (청송약수의 탄산과 유해 가능성 물질 존재에 관한 연구)

  • Lee, Sung-Ho
    • The Korean Journal of Food And Nutrition
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    • v.34 no.1
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    • pp.132-136
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    • 2021
  • The purpose of this study is to measure the levels of eluted and dissolved CO2, and CO, volatile organic substances and radiation composition of Cheongsong mineral water which were collected from November 2019 to July 2020 during the autumn, spring, and summer seasons at collection points located in the upper, middle and lower spring waters. Data of the upper, middle and lower spring waters include the following: the amount of eluted water (average value±standard deviation, mL/min) was 30.07±0.52, 15.03±0.16, 23.73±0.42, and the amount of CO2 gas was 1,000 ppm or more. In addition, there was no detection of CO or total volatile organic substances (TVOC) and the radiation dose was 0.08 to 0.13. μSv/h. A blank test value of 0.08 to 0.10 μSv/h, when compared with the median value, showed a high value of 0.02 μSv/h, and the uranium test results provided by the Cheongsong-gun Office were 0.0118 mg/L (date 2019.06.18) and 0.0091 mg/L (date 2020.06.04.) respectively, which was less than the permission limit of 0.03 mg/L. However, it is believed that further research using more precise devices is needed in order to guarantee the safety and health of the water.

Changes of Free Sugars, Lipoxygenase Activity and Effects of Chitosan Treatment during Cultivation of Soybean Sprouts (콩나물 재배중 유리당과 Lipoxygenase Activity의 변화 및 이들에 미치는 키토산 처리의 영향)

  • Lee, You-Seok;Lee, Chong-Ouk
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.115-121
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    • 1999
  • Changes of free sugars, lipoxygenase activity and effects of chitosan treatment were analyzed during cultivation of soybean sprout. Free sugars(sucrose, raffinose and stachyose) were more rapidly decomposed in the cotyledon of soybean sprout. Contents of sucrose and raffinose in control group were decreased more rapidly than those of chitosan treated soybean sprouts (CTSS). But the decreasing rate of stachyose was higher in the CTSS. In the hypocotyl, 82% of L-2/3 activity were decreased, whereas 42% of the activity were decreased in the cotyledon after 132 hours of germination. The effect of chitosan treatment on the lipoxygenase activity was more effective on L-2/3 isozyme than L-1. After the germination period of 132 hours, L-2/3 activity of control group was 82.7unit/mg and that of CTSS was 56unit/mg.

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Age and Growth Characteristic of Pacific Anchovy, Engraulis japonicus, in the Southern Waters of Korea Based on the Year Ring of Otolith and Collection Date (이석 윤문 및 채집시기를 이용해 분석된 남해 멸치(Engraulis japonicus)의 연령과 성장특성)

  • Lee, Hae Won;Hwang, Sun Do;Kim, Heeyong
    • Korean Journal of Ichthyology
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    • v.33 no.1
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    • pp.31-36
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    • 2021
  • In order to understand the age and growth characteristics of Pacific anchovy, Engraulis japonicus, the 506 otoliths of individuals collected in the southern waters of Korea in 2004 were analyzed. Monthly age of anchovies was estimated based on the age determined through the year rings and their collection date. The growth parameters obtained using the monthly folk lengths were a theoretical maximum body length of 132.9 mm, a growth coefficient of 0.1079/month and a theoretical age of 0.0262 month when the fork length is zero. The growth equation of von Bertalanffy presented by the growth parameters was Lt=132.9(1-e-0.1079(t-1.0262)). The estimation of growth parameters based on the monthly age established through year ring and collection date was deemed appropriate for understanding the growth characteristics of anchovies.

Anti-melanogenic effect of Eruca sativa extract (루꼴라(Eruca sativa) 추출물의 멜라닌 생성 저해 효과)

  • Kim, Hyun-Soo
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.74-79
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    • 2016
  • Eruca sativa (known as rocket plant) is a member of the Brassicaceae, which is considered an important chemo-preventive plant family. Although Eruca sativa has positive biological effects such as antioxidant and renal protective activities, the effect of the Eruca sativa extract as a therapeutic agent for skin whitening has not been reported. In this study, we investigated the applicability of the extract of Eruca sativa as a functional materials by examining the its physiological activities. The Eruca sativa extract showed low cytotoxicity against murine melanoma B16F10 cells. At concentrations (below 100 mg/L) that showed none or little cytotoxicity, the Eruca sativa extract showed high DPPH radical scavenging activity (ID50, 17.60 mg/L). In addition, the Eruca sativa extract inhibited tyrosinase activity ($ID_{50}$, 132.54 mg/L) and decreased melanin content ($ID_{50}$, 158.90 mg/L). Finally, the treatment with the Eruca sativa extract suppressed the protein expression of tyrosinase in a concentration-dependent manner. These findings suggested that the Eruca sativa extract inhibited melanin synthesis by not only suppressing intracellular tyrosinase expression but also directly inhibiting tyrosinase activity. Therefore, these results indicate that the Eruca sativa extract may be an effective material for functional cosmetics such as skin whitening materials.

Isolation, Identification and Characterization of Bacteria Degrading Crude Oil (원유 분해 미생물의 분리, 동정 및 특성)

  • Oh, Kyoung-Taek;Lee, Yong-Woon;Kubo, Motoki;Kim, Seong-Jun;Chung, Seon-Yong
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.10
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    • pp.1851-1859
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    • 2000
  • Crude oil-degrading bacteria were isolated from the sites contaminated by oil products. The isolates were identified as Acinetobacter sp. A132, Pseudomonas putida A422, Pseudomonas aeruginosa F721, F722, and Xanthomonas maltophilia B823. The results of investigation on the degradability of crude oil indicated that the strain A132 had the highest rate of $6.04g/L{\cdot}day$. Also, the strain A132 and F722 almost degraded each of n-alkane compounds between $nC_{10}$ and $nC_{32}$. The strain A422 degraded benzene and xylene but not n-alkane. The strain B823 grew somewhat in crude oil but did not entirely degrade other substrates used in this study. The results of the GC/FID analysis on the degradability of the mixed n-alkane compounds showed that the strain F722 could degrade 100% of the compounds with $nC_7{\sim}nC_{10}$ and more than 80% of those with $nC_{11}{\sim}nC_{24}$.

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The Flora of Higher Fungi in Mt. Jiri Areas (III) (지리산일대(智異山一帶)의 고등균류(高等菌類)(III))

  • Park, Seong-Sick;Cho, Duck-Hyun
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.132-136
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    • 1989
  • Higher Fungi were collected in Mt. Jiri National Park areas from May, 1987 to October, 1988. These higher fungi were identified. As a result of identification, Boletellus linderi Sing., Lactarius corrugis Peck, L. gerardii var. gerardii Hesler & Smith, L. gerardii var. fagicola Hesler & Smith and L. subgerardii Hesler & Smith were newly recorded in Korea.

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Feasibility of fermentative bio-hydrogen production from different organic wastes (다양한 유기성 폐자원에서 바이오 수소 생성 연구)

  • Hwang, Jae-Hoon;Choi, Jeong-A;Abou-Shanab, R.A.I.;Jeon, Byong-Hun
    • 한국신재생에너지학회:학술대회논문집
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    • 2009.11a
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    • pp.506-510
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    • 2009
  • The effects of various organic wastes on anaerobic fermentative hydrogen production were studied using enriched mixed microflora in batch tests. Rotten fruit, corn powder and organic wastewater enriched with sulfate (up to 1,000 mg/L) were used for experiments. Maximum hydrogen production (547.1 mL) was observed from rotten apple with initial substrate concentration of 132.2 g COD/L. The experimental result on sulfate enriched organic wastewater indicated that hydrogen production is not adversely influenced by relatively high sulfate concentration. Residual sulfate content remained at 96-98 % after 75 hours of reaction, which showed that no major sulfate reduction was occurred at pH 5.3-5.5 in the reactor. The volatile fatty acid (VFA) fractions produced during the reaction was in the order of butyrate > acetate > propionate in all experiments. The results of this study would be useful for controlling the conditions on fermentative hydrogen production using different feedstocks.

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